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최재혁,이용환,김효정 한국식물병리학회 2013 Plant Pathology Journal Vol.29 No.4
Rice blast fungus, Magnaporthe oryzae, inflicts serious damage to global rice production. Due to high variability of this fungal pathogen, resistance of newlyreleased rice cultivars is easily broken down. To understand the population structure of M. oryzae, we analyzed the genetic diversity of the Korean population using multilocus microsatellite typing. Eleven microsatellite markers were applied to the population of 190 rice isolates which had been collected in Korea for two decades since the 1980’s. Average values of gene diversity and allele frequency were 0.412 and 6.5, respectively. Comparative analysis of the digitized allele information revealed that the Korean population exhibited a similar level of allele diversity to the integrated diversity of the world populations, suggesting a particularly high diversity of the Korean population. Therefore, these microsatellite markers and the comprehensive collection of field isolates will be useful genetic resources to identify the genetic diversity of M. oryzae population.
Out-Phased Current Combining 방식과 Complex Combining Load 방식을 이용한 3.7∼4.0 GHz 대역 GaN-HEMT Dohert 전력증폭기
최재혁,진일비,김성형,송재성,이윤정,임서균,양영구 한국전자파학회 2023 한국전자파학회논문지 Vol.34 No.11
This paper proposes a Doherty power amplifier (DPA) with an extended output power back-off (OBO) using out-phased current combining (OCC) and complex combining load (CCL) methods. When OCC or CCL is applied independently, the output impedance of the carrier amplifier is determined to be a single value. However, when OCC and CCL are applied together, the output impedance of the carrier amplifier can be selected, which provides freedom in the circuit design. The implemented DPA achieves an extended back-off region by expanding the load impedance modulation ratio of the carrier amplifier by five times between the low-power and peak-power levels. The implemented DPA achieves 43.0∼44.3 dBm output power and 8.0∼8.5 dB gain using a CW signal at 3.7∼4.0 GHz. It achieves 72.5∼77.9 % DE at peak power and 41.5∼48.4 % DE at 9 dB OBO. Furthermore, it achieves 8.2∼8.7 dB of gain, 45.6∼50.0 % DE at 9 dB OBO, and an ACLR of −22.0∼−26.6 dBc using a 100 MHz 64-QAM 5G NR modulated signal with a peak-to-average power ratio (PAPR) of 7.8 dB.
LonWorks를 이용한 분산 지능 제어를 위한 통신 모듈의 설계 및 구현
최재혁,이태오,Choi Jae-Huyk,Lee Tae-Oh 한국정보통신학회 2004 한국정보통신학회논문지 Vol.8 No.8
본 논문은 애쉴론(Echelon)사의 LonWorks 기술을 이용하여 분산 지능 제어를 위한 LonWorks 통신 모듈 설계 및 구현에 대하여 논한다. LonWorks 통신 모듈은 하드웨어와 펌웨어(firmware)로 나눌 수 있다. 첫 번째, 하드웨어는 센서가 부착된 마이크로컨트롤러 부분과 제어와 데이터 네트워크를 함께 운용할 수 있는 LonWorks 구성요소이다. 즉, 뉴런 칩(neuron chip), 마이크로컨트롤러, 트랜시버(transceiver), LONCard로 구성되어 있다. 두 번째, 운용 펌웨어는 노드빌더(NodeBuilder) 3.0 개발 툴을 이용한 뉴런 C이다. 제작 구현된 LonWorks 통신 모듈은 LTM-10A, Gizmo 4 I/O board, 병렬 I/O 인터페이스를 이용하여 사전 테스트하였다. 그리고 필드 테스트를 위하여 마이크로컨트롤러 부분은 하이퍼터미널(HyperTerminal)을 이용하여 테스트하였고, 데이터 네트워크의 통신 절차는 윈도우즈용 LonMaker 툴을 이용하여 짧은 메시지를 송$.$수신하여 확인하였다. 이로써 LON(Local Operating Network)은 지능 디바이스를 가진 분산형 제어 기술을 사용하여 대형 제어시스템을 소형화할 수 있다. In this paper, we describes the design and implementation of LonWorks communication module for distributed intelligent control using LonWorks technology of Echelon. LonWorks communication module can be divided hardware and firmware. First, hardwares is divided into microcontroller attaching sensors and LonWorks components for working together control network and data network. Hardwares are consisted of neuron chip, microcontroller, transceiver, LONCard. Second, operating firmware is realized with neuron C using NodeBulider 3.0 development tool. Produced and implemented LonWorks communication module is pretested using LTM-10A, Gizmo 4 I/O board, parallel I/O Interface. For field test, microcontroller module part is tested by HyperTerminal, communication procedure in data network is certified by transmitting and receiving short message using LonMaker for Windows tool. Herewith, LON technology is based on network communication technique using LonWorks.
최재혁,Oleg Yarishkin,김은주,배연주,김아정,김승찬,유강현,조장훈,황은미,박재용 생화학분자생물학회 2018 Experimental and molecular medicine Vol.50 No.-
Two-pore domain K+ (K2P) channels have been shown to modulate neuronal excitability. The physiological role of TWIK-1, the first identified K2P channel, in neuronal cells is largely unknown, and we reported previously that TWIK-1 contributes to the intrinsic excitability of dentate gyrus granule cells (DGGCs) in mice. In the present study, we investigated the coexpression of TWIK-1 and TASK-3, another K2P member, in DGGCs. Immunohistochemical staining data showed that TASK-3 proteins were highly localized in the proximal dendrites and soma of DGGCs, and this localization is similar to the expression pattern of TWIK-1. TWIK-1 was shown to associate with TASK-3 in DGGCs of mouse hippocampus and when both genes were overexpressed in COS-7 cells. shRNA-mediated gene silencing demonstrated that TWIK-1/TASK-3 heterodimeric channels displayed outwardly rectifying currents and contributed to the intrinsic excitability of DGGCs. Neurotensin–neurotensin receptor 1 (NT–NTSR1) signaling triggered the depolarization of DGGCs by inhibiting TWIK-1/TASK-3 heterodimeric channels, causing facilitated excitation of DGGCs. Taken together, our study clearly showed that TWIK-1/TASK-3 heterodimeric channels contribute to the intrinsic excitability of DGGCs and that their activities are regulated by NT–NTSR1 signaling.
최재혁,문은표,백광현 한국식물병리학회 2014 Plant Pathology Journal Vol.30 No.3
Antimicrobial peptides (AMPs) are small but effectivecationic peptides with variable length. In previous study, four hexapeptides were identified that showed antimicrobial activities against various phytopathogenicbacteria. KCM21, the most effective antimicrobial peptide, was selected for further analysis to understand its modes of action by monitoring inhibitory effects of various cations, time-dependent antimicrobial kinetics, and observing cell disruption by electron microscopy. The effects of KCM21 on Gram-negative strain, Pseudomonassyringae pv. tomato DC3000 and Gram-positive strain, Clavibacter michiganensis subsp. michiganensis were compared. Treatment with divalent cations such as Ca2+ and Mg2+ inhibited the bactericidal activities of KCM21 significantly against P. syringae pv. tomato DC3000. The bactericidal kinetic study showed that KCM21 killed both bacteria rapidly and the process was faster against C. michiganensis subsp. michiganensis. The electron microscopic analysis revealed that KCM21 induced the formation of micelles and blebs on the surface of P. syringae pv. tomato DC3000 cells, while it caused cell rupture against C. michiganensis subsp. michiganensis cells. The outer membrane alterationand higher sensitivity to Ca2+ suggest that KCM21 interact with the outer membrane of P. syringae pv. tomatoDC3000 cells during the process of killing, but not with C. michiganensis subsp. michiganensis cells that lack outer membrane. Considering that both strains had similar sensitivity to KCM21 in LB medium, outer membrane could not be the main target of KCM21, instead common compartments such as cytoplasmic membrane or internal macromolecules might be a possibletarget(s) of KCM21.
최재혁,박의호,이세원,현재욱,백광현 한국식물병리학회 2017 Plant Pathology Journal Vol.33 No.1
Citrus canker disease decreases the fruit quality andyield significantly, furthermore, emerging of streptomycin-resistant pathogens threatens the citrus industryseriously because of a lack of proper controlagents. Small synthetic antimicrobial peptides (AMPs)could be a promising alternative. Fourteen hexapeptideswere selected by using positional scanningof synthetic peptide combinatorial libraries. Eachhexapeptide showed different antimicrobialspectrumagainst Bacillus, Pseudomonas, Xanthomonas, andCandida species. Intriguingly, BHC10 showed bactericidalactivity exclusively on Xanthomonas citri subsp. citri (Xcc), while BHC7 was none-active exclusivelyagainst two Pseudomonas spp. at concentration of 100μg/ml suggesting potential selectivity constrained inhexapeptide frame. Three hexapeptides, BHC02, 06and 11, showed bactericidal activities against variousXcc strains at concentration of 10 μg/ml. When theywere co-infiltrated with pathogens into citrus leavesthe disease progress was suppressed significantly. Further study would be needed to confirm the actualdisease control capacity of the selected hexapeptides.