잔디의 약배양에 있어서 살정제 처리에 의한 Larger-sized Pollen의 발생빈도 증가 및 캘러스 유도

조문수,정의동,예병쾌,안병준,최준수,Cho, Moon-Soo,Juang, Ue-Dong,Ye, Byong-Kwea,Ahn, Byung-Joon,Choi, Joon-Soo 한국식물생명공학회 2003 식물생명공학회지 Vol.30 No.1

잔디에 있어서 larger-sized pollen의 발생빈도에 대한 살정제 처리효과 및 캘러스 유기 조건을 조사하기 위하여 실험을 수행하였다. 약 안의 화분을 절취하여 화분의 생존력 및 dimorphism현상을 조사한 결과, 2가지 화분의 형태가 관찰되었다. 하나는 크기에 있어서 상대적으로 크며 (직경 30~36 $\mu\textrm{m}$) 연하게 염색되었고 크기가 작은 다른 화분 (직경 15~20$\mu\textrm{m}$)은 진하게 염색되었으며 amyloplast가 많이 산재해 있는 것으로 나타났다. 잔디의 약에서 배발생화분으로 추정되는 크기가 큰 화분 (larger-sized pollen)은 존재하고 있으나 그 수에 있어서는 약 1% 이하로 극히 적은 것으로 나타났다. 크기가 큰 화분의 발생빈도수를 높이기 위하여 약 4$0^{\circ}C$의 주간과 15$^{\circ}C$이상의 야간 온도 조건에서 재배된 잔디의 mid booting stage에서 GA$_3$50mg/L+0.1 mL/L Tween 20을 충분하게 엽면에 살포하였다. GA$_3$의 경우 mid booting stage에서 50mg/L의 농도로 처리하였을 때 크기가 큰 화분의 빈도가 25.4%로 가장 높게 나타났다. 위의 조건에서 처리된 약에서만 AA기본고체배지+8.0 mg/L 2,4-D+0.2 mg/L kinetin 배지에서 캘러스가 형성되었으나 캘러스 형성률은 약 1.0%로 매우 저조한 것으로 나타났다. In this study we investigated the effect of gametocides on the number of larger-sized pollen in anther, and also induced callus from the anther culture of Zoysia japonica Steud. Before culturing, we have observed pollens in anther through fluorescence and electron microscopes to know pollen dimorphism. There were two types of pollens observed. One type (30-36 ${\mu}{\textrm}{m}$ in diameter) consisted of vacuolated, larger-sized pollens and the other (15-20 ${\mu}{\textrm}{m}$ in diameter) smaller-sized ones with dense cytoplasm and plenty of amyloplasts. Within few hours, all the smaller-sized pollens were dead, while larger-sized ones were viable for one or two days. To induct larger-sized pollens, various gametocides were leaf-sprayed on three booting stages cultured under 4$0^{\circ}C$ /15$^{\circ}C$ (day/night) before anther culturing. Number of these larger pollens were few (less than 1%) in anther without spraying gametocides. GA$_3$increased the number of larger-sized pollens when applied at mid-booting stage. GA$_3$ with 50 mg/L treatment caused the highest percentage (25.4%) of the larger-sized pollen. Anthers with GA$_3$ treatment were only produced calli on AA medium (modified B$_{5}$+8.0 mg/L 2,4-D +0.2 mg/L kinetin), but callus formation was quite low (less than 1%).).

A heuristic approach solving the bottleneck machine problem in group technology manufacturing systems

조문수,정병희,윤창원,Jo, Mun-Su,Jeong, Byeong-Hui,Yun, Chang-Won 제어·로봇·시스템학회 1997 제어·로봇·시스템학회 논문지 Vol.3 No.5

기존에 있는 그룹테크놀로지의 방법들은 기계와 부품간의 공정유사성이나 그밖의 다른 정의의 유사성을 갖고 한 가지 정도의 알고리즘이나 수학적인 모델로써 해를 구했으나 그 해들은 실질적으로 현대 산업의 생산시스템이나 자동화에서 풀어나가야 하는 기계들의 병목현상을 풀어나가는 직접적인 접근방법을 제시하지 못했다. 따라서 본 연구에서는 새로운 기계간 유사성의 개발과 그래프이론을 응용한 알고리즘 그리고 수학적인 모델을 동시에 응용해서 병목현상을 초래하는 예외적인 원소의 재배치를 효과적이고 쉬운방법으로 제시하였다.

지역축제 이미지가 방문객 만족에 미치는 영향에 관한 연구

조문수,오상운 제주대학교 관광과경영경제연구소 2003 産經論集 Vol.18 No.-

세계자연유산 등재에 대한 제주 지역주민의 인식

조문수,박시사,노경국,오상운 한국관광학회 2008 관광학연구 Vol.32 No.3

It is widely recognized that the heritage tourism is one of the emerging markets. Recently scholars and marketers in the travel and tourism areas started to show their interest in this new sector. However the researchs on heritage tourism have rarely been conducted overseas, either have in Korea been carried out. "Jeju Volcanic Island and Lava Tubes" was inscribed as a World Natural Heritage by UNESCO at the 31st World Heritage Convention on June 27, 2007. This is the first World Natural Heritage listed in Korea. The purpose of this research is to identify the perceptions of Jeju Islanders to World Heritage enrolled. For this study, spatial proximity, birthplace, attachment and involvement as tourism players were employed to find perception variances among Jeju locals. The first findings show that residents far from heritage demonstrate positive perception compared to residents who live close to heritage. The second results indicate that natives(born in Jeju) have less negative perception. The third findings represent that locals who have more attachment display positive perception on world heritage. The last reveals that Islanders who have higher degree of involvement in tourism sectors hold more favourable perception.

제주도 항공수용력과 서비스에 관한 연구

조문수 제주대학교 관광산업연구소 2001 産經論集 Vol.15 No.2

당근 약배양체로부터 식물체 재분화

조문수,박용 大邱大學校 科學技術硏究所 1999 科學技術硏究 Vol.5 No.5

Experiments were conducted to develope the method of anther culture in carrot. Anthers were cultured in the semi-solid B5 basal media with various combinations of 2,4-D and NAA. And the cultures were kept in complete dark condition. Anthers of the most lines on the B5 basal media with 2,4-D shoed higher percentages of callus formation than those with NAA. Particularly, in line 45477, highest percentages of callus formation( 50'%) were observed on A2 medium(B5 - img/L 2,4-D 0.2mg/L BAP). Callus has regenerated into plantlets. The response of anther cultures shows a difference between lines investigated.

남한산성도립공원의 이용실태와 개발방안에 관한 연구

조문수 서울保健大學 1995 論文集 Vol.15 No.1

The purposes of this study are to analyze the actual visiting condition and to suggest a future development plan in Namhansansung Provincial Park, which is the biggest natural park in the Seoul Metropolitan area. To accomplish these purposes, a field survey and a questionaire survey were conducted. This study is composed of the following five parts; I. Introduction, II. The present condition and the actual visiting condition in the Namhansansung Provincial Park, Ⅲ. The analysis of visitor's inclinations and behavier in the Namhansansung Provincial Park, Ⅳ. The development plan for the Namhansansung Provincial Park, V. Conclusion. 1. Public facilities in the park are satisfactory compared to other tourist destinations. 2. The length of the vistor's stay is 2 hours & 54 minutes an average, which is much lower than the national average of 5 hours & 12 minutes, therefore, a counter-plan is urgently needed. 3. Complaint factors are transportation, price, and lack of recreational and public facilities. 4. The degree of satisfaction for the facilities and the service is 2.751 in general, which is slightly unsatisfactory. hypothesis 1 : The motivation for a visit and the number of a visits will have a meaningful relationship ; accepted. hypothesis 2 : The need for the development of facilities and the degree of satisfaction from the facilities and services will have a meaningful correlation; rejected. hypothesis 3 : A favorable system for the Park's development and inhabitation will have a meaningful correlation; accepted. hypothesis 4 : A favorable system for Park's the development will vary according to the degree of satisfaction; rejected. hypothesis 5 : The direction of the Park's development and a need for the development of facilities will have a meaningful correlation: accepted. Therefore, Suggestions for the development plan for the Narnhansansung Provincial Park are presented as follows ; First, A direction of the development considers regional habitants primarily as a recreation and rest area. Second, sustainable tourism development is desirable to minimize a destruction of nature and priorities for the Park's development should be set up. A mountain path, a forest bath place, an information bureau, service system, transportation in the park is the proper order for its development. Third, The third sector development system is the proper way for development, in which regional government is the main body and the central government and a private sector participates.

농학계 대학 교과과정의 문제점과 해결방안

조문수 한국원예학회 1996 원예과학기술지 Vol.1 No.2

수술장에서의 혈액 폐기율 감소

조문수,이영숙,유일미,Cho, Moon Su,Lee, Yeoung Sook,Yu, Il Me 한국의료질향상학회 1997 한국의료질향상학회지 Vol.3 No.2

Background : 6.1% of red blood cells and whole blood issued to the operating room was not transfused to the patients and discarded in Seoul National University Hospital in 1994. Objectives : We planned to set up an effective management program of blood in the operating room and we investigated whether this program could reduce the disposal rate of blood. Methods : We made a guideline of blood management in the operating room through a workshop. The guideline was revised after a preliminary application. The revised guideline was applied for 5 months from May to September in 1996. The disposal rate was compared before and after the installation of the new program. Results : 5,336 units of blood were issued to the operating room for 5 months. Disposal rate of red blood cells and whole blood was markedly reduced from 6.2% in May to 2.1% in September(p<0.05). The average disposal rate was 3.7% during the five months. Conclusion : We were able to reduce the disposal of unused blood in the operating room through the development and the application of a new blood management program.

한반도와 유라시아 물류거점 분석의 경제적 효과

조문수,유병철,강미연 한국방위산업학회 2014 韓國防衛産業學會誌 Vol.21 No.4

최근 대한민국 정부가 추진하고자 하는 통일에 대한 논의는 활발한 반면, 실질적인 접근방법에 대해서 과연 현실적인가를 고려해야 한다. 통일의 주체는 국민이며, 남북한 주민의 눈높이에 맞추어야 한다. 물류전략은 그 중에 가장 현실가능하고 중요하며, 한국방위산업의 물류거점 확보라는 의미에서 본 연구와 그 관련성이 매우 높다. 최근 북.중.러간의 물류기반산업이 활발한 것은 의식주 중에 무엇보다 먹고 사는 문제에 대해 먼저 해결하고자 하는 것이다. 본 연구는 통일을 대비하고 통일 후에 발생될 물류대란을 준비하기 위한 연구배경을 갖는다. 물동량 뿐 아니라 다양한 사회적인 물류요소를 가중화된 입력데이터로 구성하고 남북한 각 25개 물류거점을 중심으로 공학적 분석을 하고자 한다. 이를 바탕으로 유라시아까지의 물류거점이 결정된다. 이는 철도궤도의 상이함에 따라 통일물류전략의 밑바탕이 될 것으로 보며, 더불어 사회문화적인 차이를 극복하는 계기가 될 것임을 제안하였다. The realistic approaches solving for the reunification problems should have been explored based upon the people in North and South Korea. The logistic system has been one of the important factors for solving these kinds of current problems in Korea. This study examines logistics hub based on the engineering and social factors such as distance, time, cost, population, industrial development, etc., for expanding global market strategies form Korea to Europe. The logistics location and facilities, especially railroad industry in the North Korea has been explored in order to develop the Silk Road Express via Northeast areas. In this research, the coordinates of the each appropriate location have been given in terms of distance. However, the weights of each coordinates have been given by the various social factors. The assignment problem and transportation problem are also applied in the results from the Webber's multiple location problem, which the algorithm has been coded C languages. This results will be another base solving for the North Korean not only logistics systems but also the life style in the near future.