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      • 혈액보존제 ACD-B,CPDA-1 및 SAG-M에 보존된 혈액의 혈액학적,생화학적 변화에 관한 연구

        나동진,문희주,최범열 서울보건대학 1997 서울보건대학 부설 한국보건과학연구소 논문집 Vol.4 No.1

        Backgrounds ; Study as to blood preservative solution is continuousely performed to prolong period of donor blood preservation which will cope with demand increase and autologous transfusion. In Korea, CPDA-1 solution, preserved 35 days, substituded ACD-B(21days), is currently used for anticoagulant preservative solution of blood. But SAG-M solution(42days) were widely used in other contries for longer preservation as RBCs additive solution. Authors compared the survival of RBCs preserved with ACD-B, CPDA-1 anticoagulant preservative solution, SAG-M RBCs additive solution to study changes of hematological and biochemical. Materials and Methods ; Blood were collected from 15 healthy male donors aged 22 to 23years, for three sorts of preservative solution bag made in home(Company Green Cross, Korea) at the same time. 5 of them were drawn into the blood bags containing ACD-B and another 5 into the CPDA-1 bags, the other 5 donors were collected into the blood bags with CPDA-1, plasma was removed by centrifugation serparation at 4,000rpm in 5minutes and replaced by 90 ml of SAG-M additive solution. All of blood bags were stored in blood bank refrigerator at 1 to 60℃. Samples were drawn at 1, 5, 10, 15, 20, 25, 30, 35 and 42days in each through blood bags tubing under sterile condition used SCD(Sterile Connection Device) so as to measure changes of hematological and biochemical. Results ; 1) A TP level is very important preservation index of hematological test. On 1 days, ATP levels of ACD-B anticoagulant preservative solution were measured by 4.85μmol/gHb, CPDA-1 5.05μmol/gHb, SAG-M additive solution 8.91μmol/gHb. After storage for 42days ATP levels ACD-B were measured by 1.54μmol/gHb, CPDA-11.72μmol/gHb, SAG-M 2.36μmol/gHb. 2) Plasma hemoglobin in bloods stored in ACD-B, CPDA-1 and SAG-M were 24.6mg/dl, 24.3mg/dl and 16.7mg /dl, respectively, on 1days, and increased to 221mg/dl, 154.5mg/dl and 146.4mg/dl, respectively, On 42 days. 3) Another hematological study cannot be seen remarkable discrimination between three sorts of preservative solution and preservation days. 4) pH, sodium, chloride and glucose level in bloods stored in ACD-B, CPDA-1 and SAG-M were slightly descresed according to storage days, whereas pottasium and phosphorus concentration increased according to storage days, However, there was no significant difference between bloods stored in three anticoagulants. Conculusion ; In 42 days, SAG-M RBCs additive solution is more excellent than other solution(ACD-B, CPDA-1 anticoagulant preservative sol) and has merit to separate plasma from the unit blood. Therefore we concluded that SAG-M RBCs additive solution should be used to overcome the state of b, SAG-M additive solution 8.91μmol/gHb. After storage for 42days ATP levels ACD-B were measured by 1.54μmol/gHb, CPDA-11.72μmol/gHb, SAG-M 2.36μmol/gHb. 2) plasma hemoglobin in bloods stored in ACD-B. CPDA-1 and SAG-M were24.6mg/dl, 24.3mg/dl and 16.7mg/dl, respectively, on 1 days, and increased to 221mg/dl, 154.5mg/dl and 146.4mg/dl, respectively, On 42 days. 3) Another hematological study cannot be seen remarkable discrimination between three sorts of preservative solution and preservation days. 4) pH, sodium, chloride and glucose level in bloods stored in ACD-B, CPDA-1 and SAG-M were slightly descresed according to storage days, whereas potasium and phosphorus concentration increased according to storage days, However, there was no significant difference between bloods stored in three anticoagulants. Conculusion ; In 42 days, SAG-M RBCs additive solution is more excellent than other solution(ACD-B, CPDA-1 anticoagulant preservative sol) and has merit to separate plasma from the unit blood. Therefore we concluded that SAG-M RBCs additive solution should be used to overcome the state of imported plasma in our country.

      • 세포학적인 검사를 통해 자궁경부암의 조기 발견과 합리적인 병원내 산부인과의 운영 체계에 관한 고찰

        김태전,배형준,김종열 서울보건대학 1996 서울보건대학 부설 병원경영연구소 논문집 Vol.2 No.1

        In this study, the authers have investigated a true appreciation on the importance and the actual conditions of utilization of cytologic examination in gynecologic services with 3 step, that is, in 1 step the importance of cytologic examination in gynecologic services has been investigated from the results of 4,024 female of cytologic examination, in 2 step the actual conditions of utilization of cytologic examination have been investigated from 60 medical institutes through questionaire, and in 3 step the more detailed subjects of cytologic examination in gynecologic services have been investigated from 3 medical institutes selected randomly, such an investigations were done to make the programs of improvement in order to promote the activation of gynecologic medical services that shall be able to decrease occurrence of uterine cervical cancer, which is still the most prevalent cancer in korean female, and to make those on effective diagnosis and treatment activaton that shall be able to increase the income of medical institutes. The programs of improvement according to conclusions obtained from investigations like these were summarized as the following. 1. Program to activate positively cytologic examination It was well known that good correlation was noted between number of cytologic examination and those of histologic examination, those of gynecology procedures, and those of gynecologic surgeries. But it was a fact that a most of gynecology board certified physicians and physicians, and medical institutes have not utilized actively cytologic examination in gynecologic practice. Therefore all of married woman should be had politically cytologic examination at regular intervals in order to prevent and detect early uterine cervical cancer. 2. Program to establish exclusively responsible managed systems Managed systems that are exclusively responsible for cytologic examination should be established in order to have examination at regular intervals exactly(six months). 3. Program to utilize correctly the current medical insurance regulations about medical treatment charges The medical charges of cytologic exmination should be applied correctly to the current medical insurance regulations about medical treatment charges in order to increase the income of medical institutes. Because the medical charges of cytologic examination for the levels of medical check up and preventive medicin exception cancer or clinically suspicious cancer diagnosed by physicians is applicable to general medical treatment charges under the current medical insurance regulations. 4. Program to utilize reasonable diagnostic system in cytologic examination. Since certificated technologist screen first thing many microscopic slides for cytologic examination, lastly gynecologic board should make a final diagnosis on only abnormal slides. It is reasonable diagnostic system that can observe more microscopic slides, decrease misdiagnosis and high personnel expenses, and increase income in cytologic examination. Consequently it is necerssary to establish reasonable diagnostic system. 5. Program in order to promote the efficiency of the following examination and treatment system for patients to whom abnormal cell were detected. The following examination and treatment system that were corresponded with diagnosis, and automated and standardized system from receipt to gynecologic practice should be established to offer patients ease and trust, and also medical institutes the more income through the activation of gynecologic practice. Finally, the authers think that it is very important that cytologic examination should be included in routine gynecologic practice.

      • 돌연변이성에 의한 수중유기 오염물질의 독성평가

        유영식,채수권,권우택,김남천 서울보건대학 1996 서울보건대학 부설 한국보건과학연구소 논문집 Vol.3 No.1

        한강수계 팔당댐 유입 하천수를 대상으로, 다환방향족 탄화수소를 선택적으로 유리하게 흡착하는 blue-rayon을 이용하는 현수법 및 column법으로 유기물을 흡착 추출하여, Salmonella typhimurium TA 98 및 TA 100에서의 돌연변이원성을 검색한 결과 다음과 같았다. 1. 흡착 유기물량은 8월이 11월에 비하여 약 1.7배 높았고, 현수법과 column법에서의 차는 없었다. 2. TA 100 균주에서 돌연변이원성은 나타나지 않았다. 3. TA 98균주에서의 돌연변이원성은, S-9mix 첨가의 계가 S-9mix비첨가의 계보다 높았고, 11월이 8월보다 높게 나타났다. Column법이 현수법에 비하여 돌연변이원성은 예민하게 검출되었다. In order to evaluate the toxic effect of organic pollutants in aquatic environments, organic substances extracted from blue-rayon adsorbates in Kyimgan River with the hanging and column methods were assayed by the Ames Salmonella mutagenicity test. Amounts of organic extracts adsorbed with the blue-rayon hanging method and the blue-rayon column method were similar. The mutagenicity toward TA 100 strain was not shown. The mutagenicity toward TA 98 with S-9mix was higher than that without S-9mix. For the quantitative concentration of polycyclic aromatic hydrocarbons in water, it was shown that the blue-rayon column method was more efficient than the blue-rayon hanging method.

      • 우유 중 Antibiotic Residues 검출과 살균처리에 따른 영향

        양철영,이수한 서울보건대학 1995 서울보건대학 부설 한국보건과학연구소 논문집 Vol.1 No.1

        국내에서 널리 사용되고 있는 세균 발육 억제 물질 검출 시험의 일종인 dye지시약으로 2,3,5-triphenyltetrazolium chloride을 이용한 방법과 속성 검출 시험 방법인 pnzym kit를 이용한 방법으로 원유, 시유의 항생물질 검출성, 시험방법간의 검출능 비교와 살균처리에 의한 영향을 고찰한 내용은 다음과 같다. TTC 시험에 의한 원유 중의 항생물질 검출성은 3개 시험구 60시료에서 평균 25.00%의 양성율을 나타내고, 평균 시험 소요시간은 198.25분이었고, penzym 시험에 의한 양성율은 43.33%이고 소요시간은 31.35분으로 필요로 하였다. 시유 및 가공유 중의 검출성은 15시료의 TTC시험에 의한 경우 13.33%의 양성율과 penzym시험의 경우 46.66%의 양성율을 나타내고 있다. TTC시험방법에서 penicillin G는 0.03IU/ml, cloxacillin은 1.0×10^(-10)g/ml, oxacillin, ampicillin 및 sulfamethazine은 1.0×10^(-15)g/ml까지 검출이 가능하였으며 penzym 시험방법에서는 penicillin G는 0.001IU/ml, oxacillin, cloxacillin, sulfamethazine은 1.0×10^(-15)g/ml, ampicillin은 1.0×10^(-11)g/ml까지 검출성을 보였다. 100℃이내의 살균온도와 유지시간에서는 항생물질의 농도와 검출성의 차이가 발생하지 아니하였다. penzym 검출시험 방법은 특이성, 신속성, 간편성 및 재현성이 있음을 실험결과에서 나타내었다. By 2,3,5-triphenyltetrazolium-2H-chloride test method(TIC) and penzym test method, antibiotic residues in raw milk and city milk was detected, and we compared penzym test with TIC test for antibiotic detection, and we investigated the effect of holding treatment(LTLT, HTST) of various antibiotics added in 12% skim milk. The results obtained are summarized as follows; In case of raw milk, inhibitor positive were 25.00% and 43.33% of 60sample by TIC test and penzym test, and experimental require times was 198.25 minutes and 31.35 minutes, respectively. In case of market milk, inhibitor positive were 13.33% and 46.66% of 15 sample by TIC test and penzym test. The concentration of detected antibiotics by TIC test of penicillinG and cloxacillin were 0.03IU/ml and 1.0×10^(-10)g/ml, oxacillin, ampicillin and sulfamethazine were 1.0×10^(-15)g/ml. The concentration of detected antibiotics by penzym test of pencillin G and ampicillin were 0.00lIU/ml and 1.0×10^(-11)g/ml, oxacillin, cloxoacillin and sulfamethazine were 1.0×10^(-15)g/ml. The antibiotic residues were no changed on the less than l00℃ of heat temperature by TIC reduction test and penzym test. This study showed various merit such as specificity, fastness, simple handling by penzym test.

      • 응급실환자 내원행태 및 중증도에 관한 조사 연구

        유순규,최혜경,김광한 서울보건대학 1999 서울보건대학 부설 병원경영연구소 논문집 Vol.4 No.2

        To solve the problem of tertiary emergency medical center. overcrowding and long hospital stay. it is very important to analyze arrival pattern of the emergency patients who visited tertiary emergency medical center and to reviewed 312 cases of patients who visited the four places emergency medical center which emergency medicine department specialist always stays in. of Seoul and Suwon teritory, from March 1 to May 31. 1997. The results were as follow : 1. Considering sexual distribution, male patients were 60.3% and female patients were 39.7% and the most common age group was 2th decade. 2. The result of symptom severity classification of patients coming emergency medical center was urgent patients 12.8%, emergency patients 29.8%, non-emergency patients 57.4% 3. The peak time of patient's enterance in emergency medical center was between 8: 00PM and 12:00 PM(26.7%), and most patients visited between 4:00 PM and 12:00 PM (out patients coming time) 4. In a week. Monday was the most crowding day (25.0%) 5. The most patients who visited emergency medical center needed surgical care due to injury & poisoning(27.9%) and second disease of them had circulatory system (17.6%) 6. The admission rate was 42.9% and 46.4% of admitted patients were non-emergency patients. 7. The rate of the urgent patients making use of 119 ambulance was 62.5%. emergency patients rate was 47.3% 8. The treatment result of patients in emergency medical center trended improving of urgent patients(improvement rate was 72.5%)

      • 의료보호 대상자의 의료이용 실태에 관한 연구

        김윤미,김병수 서울보건대학 1997 서울보건대학 부설 병원경영연구소 논문집 Vol.3 No.1

        This study was carried out to analyze the demographic characteristics, the amounts of medical utilization and disease pattern of the medical aids. The main resource of the study was Year book of medical aids from 1992 to 1995, which were published by the Ministry of health and welfare and Korea medical insurance corporation. The results of this study were as follows. 1. As to demographic characteristics of the medical aids, 64.7% were class 2 and 35.3% were class 1. The percent of old ages above 60years was 24.9% and higher than in medical insureds. In the sex ratio, female population were much more than male. 2. In regard to medical utilizations, the amount of medical treatment fees in the inpatient by one person during 1995 was 152,740won, which was high as above 3times as in medical insureds. In the outpatient the amount was 151,165won as and high as 1.7times. The annual increasing rate of medical treatment fees was also much higher faster than in medical insureds. Medical aids admitted mainly in the general hospitals and hospitals and many utilized the public health centers in outpatient. 3. As to disease pattern, 48.9% of the inpatient claims were mental disorders and the rate of pregnancy, child birth and the puerperium disease were very low. In the case of outpatient the rate of mental disorders was 3.9% and respiratory system disease was 24.1%. digestive system disease was 17.4% and the disease of musculoskeletal system and connective tissue was 16.5%. The most frequent disease in the inpatient was schizophrenic psychoses but in medical insureds it was normal delivery. In general the rate of chronic degenerative diseases was much higher than in medical insureds.

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