의학 논문 작성법(영어)

박윤곤 대한정맥마취학회 2001 정맥마취 Vol.5 No.1

Mepivacaine의 심근수축 억제기전에 대한 기계적 및 전기생리학적인 연구

박윤곤,서창국,이행철 대한대장항문학회 2003 Annals of Coloproctolgy Vol.32 No.4

흡입마취제인 Sevoflurane 의 심근수축 억제기전에 대한 기계적 및 전기생리학적인 연구:Isoflurane 과의 비교

박윤곤,서창국 대한마취과학회 1995 Korean Journal of Anesthesiology Vol.28 No.3

Dose-related depression of left ventricular function or cardiac output has been reported in humans and in vivo animal studies with sevoflurane (SEVO) anesthesia and myocardial depressant effect of SEVO appeared to be comparable to that produced by isoflurane (ISO). This study was designed to determine the mechanical and electrophysiologic mechanism of the direct negative inotropic effects of SEVO. The effects of SEVO were comprared to those produced by equipotent concentration of ISO in the same isolated myocardial preparations. Isometric force of isolated guinea pig ventricular papillary muscle was studied in normal and 26 mM K^+Tyrode's solution. Rat papillary muscle was also used to evaluate the effect on Ca^(2+) release from the sarcoplasmic reticulum (SR) at low stimulation rates. Muscles were bathed at 36-37℃ in normal K Tyrode's solution bubbled with 95% O₂/ 5% CO₂ (pH 7.4) and were electrically stimulated following rest and at rates up to 3 Hz. Normal and slow action potentials were evaluated by using cnventional microelectrodes. Muscles were also subjected to rapid cooling (from 37℃ to 2℃) in order to elicit a transient rapid cooling contracture (RCC) known to be activatel by Ca^(2+) content released from the SR. RCCs were elicited after 2 Hz stimulation, which produced an RCC tension similar to that of the preceding contraction in control. SEVO and ISO were administered by dial setting in each vaporizer at 1.7 (1 MAC) and 3.4% (2 MAC), and 1.15 (1 MAC) and 2.3% (2 MAC), respectively. ∼20% and 40% depression of contractility was shown at 1.7 and 3.4% concentration of SEVO and the extent of depression was similar to equipotent concentration of ISO from rested state up to 3Hz stimulation rates. 1 and 2 MAC concentrations of SEVO (1.7 and 3.4%) or ISO (1.15% and 2.3%) in normal K^+ Tyrode's solution caused dose-related depression of peak force at low stimulation rates (RS, 0.1, and 0.5 Hz). Although the normal action potential (AP) amplitude or Vmax were not changed, APD_(50) and APD_(90) were prolonged characteristicly at 2 MAC of both anesthetics. Whereas no contractile depression was shown at RS and 0.1 Hz stimulation rates in rat papillary muscles, significant depression was noted from 0.5 to 3 Hz in 3.4% SEVO or 2.3% ISO. In the partially depolarized (26 mM K^+Tyrode's solution) β-adrenergically stimulated myocardium, 2 MAC concentration of both anesthetics caused selective depression of late peak in the biphasic contraction without changing early peak. In slow AP, 3.4% SEVO or 2.3% ISO did not cause any change in AP amplitude and Vmax whereas APD_(50) and APD_(90) were prolonged as in Normal APs. Rapid cooling preceded by 15 min rest showed little contractile force and marked prolongation of the time to peak contracture with almost complete absence of contracture after 2Hz stimulation rates following 3.4% SEVO or 2.3% ISO. Although complete recovery of peak force could be observed, little restoration of RCC was shown after washout for 15 minutes at 2 MAC concentration of both anesthetics characteristically. The effect ofSEVO on isolated myocardial contraction was similar to that of ISO. While neither anesthetic depressed the rapid initial Ca+ release from the SR, the depression of RCC and late tension suggest an alteration in some SR pathway. The direct myocardial depressant effects of SEVO and ISO are likely to be related to depressed Ca^(2+) influx through the cardiac memebrane, while AP prolongation may be due to actions on K^+currents.

Protamine 의 심근 독성에 대한 연구

박윤곤,홍용우,방서욱,신호선 대한마취과학회 1994 Korean Journal of Anesthesiology Vol.27 No.4

The cellular cardiac effects of protamine, the cationic polypeptide employed to reverse heparin anticoagulation, were examined in vitro to define its mechanisms of action. Isometric contractile force and action potential (AP) characteristics after rest (RS) and at frequencies up to 3 Hz were recorded in guinea pig ventricular papillary muscle. The actions of protamine (10-300 ㎍/ ml) were compared to those of heparin (10, 30 units/ml), and to heparin (10 units/ml) neutralized with equivalent (100 ㎍/ml) or excess (200 ㎍/ml) protamine. The effects of protamine were also examined using muscle rapid cooling contractures (RCCs to assess intracellular Ca^(2+) stores). Protamine (100-300 ㎍/ml) depressed contractions by 35-65% at 3 Hz, whereas contractions were enhanced 150-500% at lower rates (RS-0.5 Hz), with a concommitant rise in resting force. Protamine caused a resting depolarization from -84 to -72 mV and depressed AP amplitude. In contrast, heparin minimally altered contractile or AP characteristics. In 26 mM K^+-solution with 0.1 uM isoproterenol, 30-300 ug/ml protamine caused dose-dependent depression of late peaking force development and slow AP prolongation. After 15 minutes rest, when RCCs were not normally elicited, rest RCCs became prominent in 100-300 ㎍/ml protamine. Effects of heparin with 100 ㎍/ml excess protamine were similar to those of 100 ㎍/ml protamine alone. In conclusion the loss of normal force-frequency relation, partial depolarization, rise in resting tension, and appearance of rested state RCCs suggest that unbound protamine can lead to excess intracellular Ca^(2+), mediated by an alteration in memebrane ionic conductances.

Alpha 2 agonist의 임상적 사용

박윤곤 연세대학교 의과대학 1994 연세의학 Vol.1 No.-

Mepivacaine의 심근수축 억제기전에 대한 기계적 및 전기생리학적인 연구

박윤곤,이행철,서창국 대한마취과학회 1997 Korean Journal of Anesthesiology Vol.32 No.4

Background: The effects of various concentration (20, 50, 100? M) of mepivacaine were studied in isolated guinea pig and rat right ventricular papillary muscles by measuring the effects on myocardial contractility and electrophysiological parameters. Methods: Isometric force of isolated guinea pig ventricular papillary muscle was studied in modified normal and 26 mM K+ Tyrode's solution. Rat papillary muscle was used to evaluate the effect on Ca2+ release from the sarcoplasmic reticulum (SR) at low stimulation rates. Normal and slow action potentials (APs) were evaluated by using conventional microelectrode technique. Rapid cooling contractures (RCCs), an index of SR Ca2+ content, which are known to be activated by Ca2+ released from the SR were performed. Results: Mepivacaine caused dose-dependent depression of peak force from 0.5 to 3 Hz stimulation rates in guinea pig papillary muscles. Conduction block was frequently noted especially at higher stimulation rates (2 and 3 Hz) at all concentration ranges. In rat, ∼20% depression of peak force was shown at rested state contraction. Shortening of AP duration and rate-dependent depression of dV/dt max could be observed at 100 M mepivacaine. In 26 mM K+ Tyrode's solution, 50 and 100 M mepivacaine caused dose-dependent depression of early and late force development. In slow APs, neither shortening of AP duration nor changes of dV/dtmax were not shown at 100 M mepivacaine. ∼30% depression of RCC after 2 Hz stimulation rate was shown at 100 M mepivacaine. Conclusion: It may be concluded that the direct myocardial depressant effects of mepivacaine may partly be related to inhibition of Ca2+ release from the SR. Shortening of AP duration in normal APs seems to be partly related by blockade of TTX-sensitive $quot;window$quot; Na+ current. (Korean J Anesthesiol 1997; 32: 491∼503)

한국의 행정문화

박윤곤 경영행정대학원 1992 최고경영자과정 논문집 Vol.1 No.-

네트워크 취약점 공격의 효율적인 탐지를 위한 Snort 규칙 분석과 개선

박윤곤,조호성,박희진 한국정보과학회 2012 한국정보과학회 학술발표논문집 Vol.39 No.1C

지속적 미골절단에 의한 동통관리 3례

박윤곤(Wyun Kon Park),윤덕미(Duck Mi Yoon),오흥근( 대한통증학회 1989 The Korean Journal of Pain Vol.2 No.1

N/A We experienced 3 cases of continuous caudal block. The first case had suffered from severe pain of the external genitalis after urethral injury from a car accident and this was controlled by continuous caudal block. The other 2 cases were a metastaric malignant tumor of the lumbar vertebra from cancer of the cervix and histiocytoma of the breast, and both had suffered from intractable pain of the lower extremity. But lumbar epidural block was impossible because of radiation fibrosis and previous operation scar of the spine. So a continuous caudal block was performed and the pain was controlled effectively. The longest duration was 50 days and there were no problems related catheter indwelling. Pain in the area of the lumbar and sacral nerve distribution can be controlled by continuous caudal block. Here in we reported 3 cases and reviewed the literature.

제왕절개술 마취시 동맥혈과 호기말 이산호탄소 분압차이의 비교

엄대자,박윤곤,임현교,윤경봉,최령 대한마취과학회 1991 Korean Journal of Anesthesiology Vol.24 No.5

Studies were made to ascertain the relationship between arterial and end-tidal carbon dioxide tension in 19 patients during caesarean section under general anesthesia(Group 2). 27 nonpregnant patients scheduled for abdominal hysterectomy were also performed as a control group(Group I). 15 and 30 minutes after the induction of anesthesia in group I, and just before uterine incision, 15 minutes following induction in group 2, arterial blood was sampled for PaCO analysis and PETCO₂(end-tidal CO₂ tension) determined at the same time. In group 1, there was a statistically significant differences between PaCO₂ and PETCO₂ but in group 2, no differences between them were shown. The highly significant correlations between PaCO₂ and PETCO₂ in both groups were found. On the basis of the above results, we recommend that non-invasive measurement of PETCO₂ can be used reliably in stead of measuring PaO₂ to evaluate the ventilatory status during anesthesia in patients for caesarean section.