[Oral Presentation 4] 구강편평세포암종에서의 제1막형 금속함유 단백분해효소의 발현

명훈,홍성두,이재일,임창윤,홍삼표 대한구강악안면병리학회 2001 대한구강악안면병리학회지 Vol.25 No.2

구강편평상피세포암 세포주와 골육종 세포주에서 Taxol?怜? Cisplatin의 항암 효과에 대한 연구

명훈(Hoon Myoung),김명진(Myung Jin Kim) 대한구강악안면외과학회 1999 대한구강악안면외과학회지 Vol.25 No.3

Object Docetaxel (TaxolR), which has been used for breast cancer and ovarian cancer and cisplatin were applied to KB and HOS cell lines. Relative cytotoxicity of each drug to cell lines and its efficiency was evaluated and analyzed for clinical application. Method and Material Groups were defined by drugs and cell lines combination and were evaluated. We cultured cell line under routine environment and determined optimal cell number and drug concentration. Drugs were applied to cell lines and we measured the optical density with ELISA after MTT application. Statistical analysis was made and clinical availability was evaluated. IC50 and assay AUC could be calculated. Clinical availability was evaluated and comparisons of anti-tumor effect of each drug on cell lines were performed. Results 1. Anti-tumor effects of taxol and cisplatin on each cell lines showed time and dose dependent relations with significance statistically. 2. Assay AUC in IC50 was higher than clinically achievable AUC in all groups except cisplatin-KB group. Anti-tumor effect of taxol to KB cell line was higher than that of cisplatin in concentration calculated from the clinically achievable AUC. So, we could suggest that taxol could be also used as a antitumor durg for oral cancer. Further study that is, in vivo study, evaluation of combination therapy with other drug, and method of increasing of AUC would be necessary.

구강편평세포암종 이식 누드마우스에서의 혈액 점도 변화

명훈(Hoon Myoung),이종호(Jong-Ho Lee),정필훈(Pill-Hoon Choung),김명진(Myung-Jin Kim) 대한구강악안면외과학회 2004 대한구강악안면외과학회지 Vol.30 No.2

It is well known that malignant tumor have hypoxic cell fraction, which is radio resistant and is one of the most important cause of local recurrence after radiotherapy. One of the causes of hypoxia in tumor is blood flow decrease due to increase in blood flow resistance and one of the causes of increased blood flow resistance could be attributed to the increase in blood viscosity. For the evaluation of the change of blood viscosity in oral cancer, experiments were carried out to test the change of blood viscosity among the normal control and xenografted oral cancer nude mice. Relative viscosity measured against distilled water was 3.30±0.14 for normal control, and 3.67±0.62 for tumor bearing mice at the first time of blood sampling in experimental period (100 mm3 〈tumor volume〈200 mm3). There was no statistically significant difference between the control group and experimental group (p〉0.05). However, as the tumor grew, significant difference of blood viscosity was detected at the third time of blood sampling (control group:3.37±0.59, and experimental group: 4.31±0.41 300 mm3 〈tumor volume, p〈0.05). Increase in blood viscosity was confirmed with this experimental study and it can be postulated that modification blood viscosity might contribute to decrease of hypoxia fraction in oral cancer, thus improve the effect of radiotherapy.

Genistein을 투여한 햄스터 협낭 구강암 모델에서의 Vascular Endothelial Growth Factor (VEGF) 발현 변화에 대한 면역조직화학적 연구

명훈(Hoon Myoung),김영연(Young-Yun Kim),최상묵(Sang-Mook Choi),정종평(Chong-Pyoung Chung),김명진(Myung-Jin Kim) 대한구강악안면외과학회 2002 대한구강악안면외과학회지 Vol.28 No.2

Genistein that is a component of soy has been reported to have a protective effect on the carcinogenesis of various tumors and to inhibit the growth of a wide variety of tumor cell in vitro. Angiogenesis is an essential process for the carcinogenesis, growth, invasion and metastasis of cancer and genistein has been suggested to act as natural anti-angiogenic agent. The purpose of this study was to evaluate the effects of genistein on the vascular endothelial growth factor (VEGF) expression in hamster buccal pouch oral carcinogenesis model induced by 9, 10-dimethyl 1,2-benzanthracene (DMBA). Experimental group that were supplied with 0.1mg/day genistein were sacrificed by time schedules and routinely processed for immunohistochemical examination of VEGF. In genistein treated group, carcinogenesis was retarded with respect to the acanthosis, hyperkeratosis, and epithelial dysplasia. Immunohistochemical study showed that the VEGF protein of genistein group was less expressed than that of the control group. (p<0.05) Thus, it is postulated that genistein has chemopreventive effect on the oral carcinogenesis, and this chemopreventive effect, at least partly, is originated from the anti-angiogenic effect of genistein

화학 발광 검사법을 이용한 구강 편평 태선의 진단

명훈(Hoon Myoung),정해석(Hae-Seok Jeong),이현선(Hyun-Sun Lee),홍순민(Soon-Min Hong),김명진(Myung-Jin Kim) 대한구강악안면외과학회 2007 대한구강악안면외과학회지 Vol.33 No.4

Purpose: This study was conducted in order to evaluate how chemiluminescent lighting (ViziLite) could increase the sharpness of margin and contrast to normal mucosa in the diagnosis of oral lichen planus (OLP), the most frequent oral premalignant lesion, compared with direct visual inspection under incandescent light. Methods: 41 consecutive patients, diagnosed to have OLP with visual inspection under incandescent light, were further examined with chemiluminescent light. The degrees of margin sharpness and lesion contrast were ranked on a scale from 1 to 3 for all patients under visual inspection and chemiluminescent light. The presence of additional lesion only detected by chemiluminescent light, complication, and discomfort were checked for each patient. After both screening tests, biopsy specimens were harvested from all patients with scalpels and histopathologic assessments were done. Results: All 41 patients were diagnosed to have OLP by both visual inspection and chemiluminescent light examination. This result was definitively diagnosed by histopathology. Degrees of margin sharpness and lesion contrast were increased by chemiluminescent light compared with visual inspection, but only the difference of lesion contrast was statistically significant. In 22.0% of patients, additional lesions were detected and 88.9% of them were diagnosed to have OLP histopathologically. 17.1% of patients noted discomfort and 9.8% of patients showed complications after chemiluminescent test. Conclusion: Chemiluminescent light may not be proper for the screen test of oral cancer or premalignant lesion but showed some possibility for additional diagnostic tool for definitively diagnosed patients in determination of lesion margin and scope

유동적 결합성을 가지는 다중프로세서의 구조와 성능분석

선우 명훈(Sunwoo Myung Hoon) 한국정보과학회 1994 정보과학회논문지 Vol.21 No.12

일반적으로 다중프로세서에서 프로세서들간의 데이타 공유를 위하여 두가지 방식이 주로 쓰이는데 이는 메시지 전달 방식 및 공유 기억소자 방식이다. 그러나 전자는 통신부담이 결점이며 후자는 공유기억소자 분쟁의 단점이 있다. 또한 비효율적인 데이타 입출력 구조도 성능향상에 있어 제한사항이다. 본 논문에서는 위의 단점들을 경감시킬 수 있는 유동적인 연결구조를 갖는 새로운 다중프로세서 (Flexibly Coupled Multiprocessors)를 제안한다. 이 다중프로세서의 구현을 위하여 가변길이 기억소자 (Variable Address Space Memory)를 제안하는데 이는 이웃한 기억소자 모듈들이 필요에 따라 재분할이 가능한 버스구조에 의하여 하나의 기억소자 모듈이 될 수 있다. 따라서 이 가변길이 기억소자 모듈들은 공유 기억소자 또는 비공유 기억소자로 사용된다. 제안하는 다중프로세서의 정량적 모델을 정립, 분석하였고, 상용의 하이퍼큐브 구조 다중프로세서인 Inter's Personal SuperComputer (iPSC)를 이용하여 시뮬레이션 하였다. 성능평가를 위하여 병렬처리 알고리즘들을 개발하여 실지로 iPSC상에서 구현하였으며 제안하는 다중프로세서의 정량적 모델을 이용, 시뮬레이션을 통한 비교 분석을 수행하였다. 실험결과 제안하는 다중프로세서가 iPSC보다 성능에서 월등함을 입증하였다. Two hardware mechanisms are mainly used for data sharing among processors in existing multiprocessors: message passing in loosely coupled multiprocessors and shared memory in tightly coupled multiprocessors. The former has communication overhead and the latter has shared memory contention. Moreover, inefficient data input and output (I/O) schemes are also limitations on performance. This paper proposes a new architecture, called the Flexibly (Tightly/Loosely) Coupled Multiprocessors (FCM), to alleviate these disadvantages. A variable address space memory scheme, in which a set of adjacent memory modules can be merged by a dynamically partitionable bus, is proposed to realize FCM. Computational models of FCM are established, quantitatively analyzed and simulated on the iPSC (Intel's Personal SuperComputer), a hypercube multiprocessor. Parallel algorithms for region labeling and median filtering are implemented on the iPSC and simulated on FCM. The performance of FCM shows remarkable improvement over that of the iPSC.

『대승기신론광석(大乘起信論廣釋)』의 사위(四位) 고찰 -『능가경(楞伽經)』의 사종선(四種禪) 인용 및 회통의 타당성 검토

이숙영(명훈) 불교학연구회 2024 불교학연구 Vol.78 No.-

담광(曇曠, 생몰년 미상)의 사위(四位) 해석은 7권본『능가경』(이하, 『7권경』)의 사종선을 배대하여 재해석하는 특징을 가지고 있다. 그러나 사위와 사종선의 배대는 다른 『기신론』 주석서들에서는 찾을 수 없어 담광의 독자적 해석이라 할 수 있다. 따라서 본고는 담광의 사위 해석을 검토하고 사종선 인용 및 회통의 타당성에 대해 살펴보고자 하였다. 먼저 사종선의 의미와 활용에 대해 검토하였다. 사종선은『능가경』문헌을 제외하고는 화엄이나 선종 문헌에서 인용되었는데, 화엄에서는 사종선을 보살 십지에 대응시키려 시도하였으며 선종에서는 여래선이라는 용어를 인용한 것이 확인되었다. 다음은『광석』의 사위에 대한 고찰이다. 양 본의 사위 비교 및『광석』의 사위 해석을 살펴본 결과 담광은 양 본이 궁극에 지향하는 것이 같음에도 불구하고 회통을시도하였다고 판단하였다. 그러나 이는 S본의 사위가 유념과 무념으로 설명되고 있기 때문에 담광이 무념을 포괄하여 해석하기 위해 사종선을 인용하여 극복하려 했을 것으로 추정된다. 8세기 담광이 활동하던 시기는 하택신회나 마조도일 등이 활약하며 여러 선종 문헌이 등장하던 시기였다. S본의 경우 사위 해석에 ‘무상’을 강조하거나 초상에 관해 ‘마음도 오히려 없는 것’이라 말하는데 이것은 당시의 사상적 흐름과도 관련이 있다고 생각된다. 따라서 원효나 법장, 초기 선종에서 중시하던 『능가경』은 담광에게도 영향을 미쳤을 것으로 판단된다. 한편 담광이『7권경』과 S본의 역자가 같다고 간주했을 경우,『7권경』에서 회통의 근거를 찾은 것은 당연한 시도였다고 본다. 특히 사종선과 사위는 각 단계에서 동일한 수행 주체와 성취를 보이기 때문에 서로 배대하는 것에 무리가 없었을 것으로 판단된다. 『광석』은 8세기 중반의 주석서이다. 당시 돈황은 당의 영향 아래 있었기 때문에 주석 시 시대적 영향도 함께 받았을 것으로 생각된다. 따라서 『광석』은 8세기의 사상적 흐름 및 시대성을 띤 『기신론』 주석서로서 충분한 연구적 가치가 있다고 생각한다. Tankuang (曇曠)’s interpretation of the four stages is characterized by a comprehensive interpretation citing the four types of Chan (J. Zen) in the Laṅkāvatāra-sūtra in seven volumes. However, this interpretation is only found in Dashengqixinlun guangshi (hereafter, Guangshi). Therefore, this paper examines the validity of citing the four types of Chan and comprehensive interpretation through Tankuang’s the four stages interpretation. After comparing the four stages in the two versions of The Awakening of Faith in the Mahāyāna and examining Guangshi’s interpretation, I determined that Tankuang attempted to reconcile these perspectives, even though the ultimate goal was the same in both versions. However, this may be due to the fact that the four stages in the Śikṣānanda’s version are described as misleading thoughts (妄念) and no-thought (無念); thus Tankuang cites the four types of Chan to interpret “no-thought” inclusively. Tankuang was strongly influenced by Wŏnhyo (元曉) and Fazang (法藏) in writing the Guangshi and Tankuang’s period of activity in the eighth century was a time when many Chan texts were appearing by Shenhui (神會), Mazu (馬祖), and others. Śikṣānanda’s version emphasizes “no-form (無相)” in the interpretation of the four stages and states “there is not mind” in the interpretation of the first form (初相), which is also thought to be related to trends in thought at the time. Thus, the Laṅkāvatāra-sūtra which was emphasized in the early Chan schools of Wŏnhyo (元曉) and Fazang, may have influenced Tankuang. Furthermore, if Tankuang considered the translator of Śikṣānanda’s version to be the same as the translator of the Laṅkāvatāra-sūtra in the seven volumes, then this reflected a natural attempt to find a basis for unity in the Laṅkāvatāra-sūtra translation in the seven volumes. In particular, both sets of four stages have the same disciplinant and accomplishments at each stage, therefore, it is unreasonable to connect them.

Genistein이 햄스터 협낭 구강암 모델에 미치는 암예방 효과

김영연,명훈,김명진,Kim, Young-Youn,Myoung, Hoon,Kim, Myung-Jin 대한구강악안면외과학회 2001 대한구강악안면외과학회지 Vol.27 No.2

Recently, the consumption of soy products has been associated with low rates of hormone-dependent and hormone-independent cancers. Asians, who consume $20{\sim}50times$ more soy per capita than Americans, have lower incidence and death rates from breast and prostate cancer. Because soy contains the isoflavones genistein and daidzein (present as their glycosidic conjugates) at mg/g concentrations, it has been suggested that isoflavones might be acting as natural chemopreventive agents. During the 1980s several groups of investigators carried out experiments to test the effectiveness of soy in the diet in animal models of cancer. These studies reported a protective effect of soy; none showed that soy increased cancer risk. Genistein was shown to inhibit the growth of a wide variety of tumor cell types in culture. The purpose of this study was to evaluate the effects of genistein on the carcinogenesis induced by topical application of 0.5% 9, 10-dimethyl 1,2-benzanthracene (DMBA) on the hamster buccal pouch. 48 syrian hamsters were employed in this study, divided into experimental group and control. 24 animals (DMBA topical application group) had the right buccal pouch painted 3times weekly with 0.5% DMBA in mineral oil, 24 animals (genistein group) were supplied with 0.1mg genistein with DMBA topical application. 3 animals in the experimental group and control were sacrificed at serially each other week after experiments. Their buccal pouches were removed and routinely processed for microscopic examination. The results were as follows: 1. In DMBA topical application and genistein group, they showed carcinogenesis as time goes by experimental stage. 2. Genistein group was retarded in carcinogenesis related to the acanthosis, hyperkeratosis, epithelial dysplasia. 3. p53 immunohistochemical study showed that the p53 protein of genistein group was less expressed than that of the control group. Thus, it seems that genistein has chemopreventive effect on the carcinogenesis in the oral cavity, but further study is required to elucidate the anticancer mechanism of genistein.

Genistein이 사람 섬유육종 세포주 증식 및 Membrane Type 1-Matrix Metalloproteinase (MT1-MMP) mRNA 발현에 미치는 영향

강진한,명훈,김명진,Kang, Jin-Han,Myoung, Hoon,Kim, Myung-Jin 대한구강악안면외과학회 2001 대한구강악안면외과학회지 Vol.27 No.4

Matrix metalloproteinases have long been viewed as ideal candidates for proteinases that enables tumor cells to permeated basement membrane defenses and invade surrounding tissue. There is growing evidence that the MMPs have an expanded role, as they are important for the creation and maintenance of a microenvironment that facilitates growth and angiogenesis of tumors at primary and metastatic sites. MT-MMPs are not secreted but instead remaining attached to cell surfaces. Although not all of the MT-MMPs are fully characterized, MT-MMPs have important role in localizing and activating secreted MMPs. The MMP genes are transcriptionally responsive to a wide variety of oncogene, growth factors, cytokine, and hormones. Currently, a number of MMP inhibitors are being developed and some have reached clinical trials as anti-metastatic or anti-cancer therapies. MT1-MMP is involved in the activation of proMMP-2. MT1-MMP is significant not only as a tumor marker but as a new target for chemotherapy against cancer. The purpose of this study was to evaluate the effects of protein kinase C inhibitor(genistein) on the proliferation of HT1080 and expression of MT1-MMP mRNA. Human fibrosarcoma cell line HT1080 was cultured and divided 2 groups. The experimental group was treated with $100{\mu}M$ genistein and incubated 12h, 24h for $[3^H]-thymidine$ uptake assay and northern hybridization individually. And the control group was treated with same amount of PBS for the above procedures. $[3^H]-thymidine$ incorporation was measured with ${\beta}$ ray detector. And RT-PCR and northern blotting for MT1-MMP mRNA was performed. The results were as follows 1. $[3^H]-thymidine$ uptake was reduced in experimental group with statistical significance. 2. MT1-MMP mRNA expression was significantly reduced in experimental group. These results showed that protein kinase C inhibitor (genistein) inhibited proliferation of HT1080 and almost completely blocked transcription of MT1-MMP mRNA. So, it is possible to use the protein kinase inhibitor (genistein) as anti-metastatic and anti-proliferative agent.

임플란트 식립을 위한 치조골 증강술의 임상적 분석

팽준영,명훈,황순정,서병무,최진영,이종호,정필훈,김명진,Paeng, Jun-Young,Myoung, Hoon,Hwang, Soon-Jung,Seo, Byoung-Moo,Choi, Jin-Young,Lee, Jong-Ho,Choung, Pill-Hoon,Kim, Myung-Jin 대한악안면성형재건외과학회 2006 Maxillofacial Plastic Reconstructive Surgery Vol.28 No.4

Objectives: Distraction osteogenesis has recently evolved a challenging technique to overcome the limitations of conventional augmentation procedures. The aim of this report was to evaluate the clinical result of alveolar distraction osteogenesis for implant installation. Methods: Twenty five patients with alveolar ridge deficiencies were treated with vertical alveolar distraction osteogenesis by intraoral device (total 27 devices: 25 extraosseous and 2 intraosseous devices). After the latency periods of 5-7 days, activation of the device was started. The distraction rhythm and rate was 0.75-1.0 mm a day with 2 or 3 times a day. After 3-4 months, dental implants were placed with removing the distractor simultaneously. Results: On average, a vertical gain of $9.8{\pm}3.4\;mm$ was obtained by distraction osteogenesis. Total 84 implants were installed. Average follow up period was $13.5{\pm}7.5$ months. No implant was removed during the follow up period. Three patients showed infection during the distraction osteogenesis. Three devices were broken and 2 devices among them were replaced with new one. Conclusion: Relatively larger amount of alveolar bone augmentation could be obtained with distraction osteogenesis. For the ideal anatomically and functionally ideal regeneration of alveolar bone to install dental implant, the complication of distraction should be controlled.