포도 Muscat Bailey A의 安定生産을 위한 Gibberllin 處理試驗 (Ⅱ)

裵基煥 진주산업대학교 1981 論文集 Vol.19 No.-

포도 品種은 開花期의 날씨가 고루지 못할때 無處理栽培를 하면 受精不良으로 송이가 단단하지 못하여 商品價値가 아주 低下되나, Gibberellin處理를 하면 果粒의 肥大와 着粒數의 增加로 果房이 단단하여진다. 그러나 今年은 多幸히 開花期의 好條件으로 無處理區의 송이도 단단하여 處理區間에는 有意差가 없었다. 品性向上을 期하기 위하여, 前年에 이어 Gibberellin의 單回處理의 適期를 모색코져, 100ppm 濃度로서 滿開後 5日(G_1區), 10日(G_2區), 15日(G_3區), 20日(G_4區)의 處理區를 두어, 그 處理가 果房에 미치는 影響을 調査하였던 바, 다음과 같은 結果를 얻었다. 1. 熟期는 處理期가 無處理區에 비하여 有意的으로 促進되나, 不過 1∼2日에 지나지 않는다. 2. 果房長은 無處理區에 비하여 G_1 區만이 길고, 其他의 處理區는 有意差가 없었다. 3. 果房重은 處理區가 모두 無處理區에 비하여 무겁고, 各 處理區間에는 有意差가 없으나, G_4區는 다른 處理區에 비하여 多少 가벼운 傾向이 있다. 4. 着粒數는 無處理區에 비하여 G_1 區만이 많고, 其他의 處理區는 有意差가 없었다. 5. 平均顆粒重은 G_1 區를 除外하고는 各 處理區가 모두 無處理區에 비하여 무거웠고, 이들 各 處理區間에는 有意差가 없었다. 6. 果房內 4g 未滿의 小粒含有率은 無處理區에 비하여 G_1 區는 높으나, 반대로 G_2區, G_3區, G_4區 各 相互間에는 有意差가 없었다. 7. 以上의 今年度의 實驗結果로서는 處理適期는 滿開後 10日부터 20日 사이에 있으나, 收量面으로 볼 때 滿開後 10日부터 15日 사이에 處理하는 것이 더욱 安全하리라 생각된다. In oredr to find the best time of gibberellin application for stable production from climatic danger and for improvement of market merit with Bailey A grapes, this study was designed continuously following previous test. GA_3 at 100 ppm concentration was applied only once after full bloom by dipping the clusters. The designed treatments were the following 4 kinds: G_1 treatment applied on the 5th day, G_2 treatment on the 10th day, G_3 treatment on the 15th day, and G_4 treatment on the 20th day after full bloom. The results were as follows: 1. The maturities in all treatments were forced by one or two days compared with that in untreatment. 2. The clusters’s length in G_1 treatment was long, but those in the other treatments were not different significantly, compared with that in untreatment. 3. The cluster’s weights in all treatments were heavy compared with that in untreatment, but significan diffrences were not shown between those in these treatments. 4. The number of berries per cluster in G_1 treatment was largy, but those in the other treatments were not diffrent significantly, compared with that in untreatment. 5. The mean weihgts of berry in the other treatments except G_1 tretment were heavy compared with that in untreatment, and then signiiftcant diffrences were shown between those in those treatments. 6. The percentage of light berries not exceeding 4 gr. in G_1 treatment was remarkbly high, but, on the contrary, those in G_2, G_3, and G_4 treatments were remarkbly low, compared with that in untreatment, and then significant differences were not shown between those in G_2, G_3, and G_4 treatments. 7. The results of this study showed that the practical application period was from the 10th day to 20th day after full bloom, but it was conceivable that application from 10th day to 15th day was better suitable because of tendency to had heavy yield compared with that in application on the 20th day after full bloom.

은행잎 플라보놀배당체에 대한 셀루라제류의 영향

배기환,민병선,백흠영,안병준 충남대학교 암연구소 1991 癌共同硏究所 硏究誌 Vol.1 No.1

The extractability and stability of ginkgoflavonolglycosides under presence of several cellulase preparations were investigated. The enzymes used were macerosin, cellulase C and cellulase NC. The content variation of the glycosides was measured with HPLC method, using caffeic acid as an internal standard. The methanol extract of ginkgo leaf, containing the total flavonolglyco-sides of 4.46%, was used for the content comparison. By extraction with the enzymes, each or mixed, the peak levels of all the glycosides began to decrease after 1 or 2 hours. After 24 hour extraction, most of the glycosides were degraded to minor components. The flavonolglycosides in ginkgo leaf were also hydrolysed simply by the water extraction. After 24 hour extraction with water at 40°C, the peak levels of major glycosides were distinctly decreased. Rutin was hydrolysed by enzyme treatment or by ginkgo leaf itself. As a result, it was concluded that the commercially available cellulases and the ginkgo leaf itself contain the activities of β-glycosidase and α-rhamnosi-dase. Kaempferol-3-0-(6' -0-p-coumaroylglucosyl)-rhamnoside and four other ginkgo flavonolglycosides were not hydrolysed under the same condition."

7,8-Dioxo-A-norerythrinan의 합성

배기환,서원준 충남대학교 약학대학 의약품개발연구소 1994 藥學論文集 Vol.10 No.-

7,8-Dioxo-A-norerythrinan (A) was synthesized from acid catalyzed cyclization of 6a-hydroxy-1-(2-phenylethyl)-octahydrocyclo-penta[b]pyrrole-3a-caboxylic acid ethyl ester (B) with concomitant deethoxycarbonylation. The intermediate (B) was a hydroxylated compound of N-acyliminium (C). The unstable pyrrolinium (C) was prepared from oxalylation of the enamine of phenethylamine and ethyl 2-oxocyclopentanecarboxylate.

4,4'-Biphenol 유도체의 합성 및 충치균 Streptococcus mutans OMZ 176에 대한 항균작용

배기환,서원준,임승희 충남대학교 약학대학 의약품개발연구소 1992 藥學論文集 Vol.8 No.-

In a continuous study for the developing of the anticariogenic agents, 3,3'-diacyl-4,4'-biphenol derivatives (Fig.2, 2 and 3) and 3,3'-bis-(l-hydroxyalkyl)-4,4'-biphenol (Fig.3, 4 and 5) derivatives are synthesized successively from 4,4'-diphenol (Fig.2, l). The synthesized compounds are tested for their antibacterial activity against a cariogenic bacterium, Streptococcus mutans OMZ 176. The acyl derivatives, 2 and 3, do not show antibacterial activity, but the hydroxyalkyl derivatives, 4 and 5, reduced from the acyl group of 2 and 3, show the activity. The antibacterial activity of 2 and 3 may be inhibited due to intramolecular hydrogen bonding between the acyl group and the hydroxyl one (Fig.4).

L1210 세포에 대한 약용 식물의 세포독성 검색

배기환,민병선,도동선,김남수,양기종,안병준 충남대학교 약학대학 의약품개발연구소 1992 藥學論文集 Vol.8 No.-

For the research of cytotoxic natural products, 50 medicinal plants were extracted with benzene and methanol, separately, and screened against L1210 cells. From the results(Table I), 6 samples showed cytotoxicity both in benzene and methanol extracts of 17 samples in benzene extracts and 3 samples in methanol extracts, respectively. Generally, the cytotoxicity exhibited high frequency (34%) in benzene extract but low frequency in methanol extract(6%), it meant that active cytotoxic components had less polarity. ED_50 values less than 10 ㎍/㎖ were observed in 17 medicinal plants.

대추로부터 베튜리닉 산과 알피톨릭 산의 분리 및 정량

배기환,이상명,이은실,이준성,강종성 충남대학교 약학대학 의약품개발연구소 1996 藥學論文集 Vol.12 No.-

Betulinic acid and alphitolic acid, the triterpenoids of Zyziphi Fructus, were isolated with silica gel column chromatography and used as the standard substances for the analysis. The compounds were determined with HPLC and HPTLC. They were separated on reversed phase coumn (Nova-Pak C_18) with 0.05 M Na_2HPO_4-methanol (19:81) in HPLC and detected at 210 ㎚. Separation on HPTLC precoated sillica gel F_254 plates was carried out with chloroform-methanol (6:1) and the separated compounds were reacted with ρ-anisaldehyde and detected at 540 ㎚. The contents of betulinic acid and alphitolic acid in Zyziphi Fructus from four different regions in Korea were in the range of 2.9~3.8% and 3.2~3.9%, respectively.

棉實의 筋收縮, 血壓降下 및 過血糖作用

배기환 충남대학교 자연과학연구소 1984 忠南科學硏究誌 Vol.11 No.1

The purpose of this study was to investigate the action of water extract of Gossypium Semen(GWE). It was clarified that GWE caused the contraction of an isolated frog's rectus abdominis muscle, the fall of the blood pressure in a rabbit, and the hyperglycemia in a hen.

생약으로부터 충치예방 및 치료제 개발

배기환 영남대학교 약품개발연구소 1993 영남대학교 약품개발연구소 연구업적집 Vol.3 No.-

One hundred and fifty kinds of crude drugs were tested for their antibacterial against a main cariogenic bacterium. Streptococcus mutants OMZ176. Among the crude drugs, the methanolic extracts of Magnolia ovovata, Liriodendron tulipifera, Reynoutria elliptica, and Commelina communis showed strong inhibitory activaty on the growh of S. mutans. The fractonation of the extracts, monitored with the bioassay, resulted in the isolation of the active components. Magnolol and honokiol were isolated from harman and nonharman from C. communis. The chemical structure and activity relationships are also discussed.

비선형 근사필터에 강구조시험체의 지진응답추정 및 동특성식별

배기환,김두영 한국지진공학회 2001 한국지진공학회논문집 Vol.5 No.2

대상으로 하는 시스템의 입출력신호에 근거하여, 시스템의 수학적 모델을 결정하는 것을 총칭하여 시스템식별이라 한다. 본 논문에서는 지진응답 관측치를 입출력신호로 하여 조건부대치를 최적치로 판단하는 비선형근사필터법을 사용한 건축구조물의 지진응답추정 및 파라미터식별에 관하여 논한다. 비선형근사필터법에 의한 건축구조물식별의 유효성의 적용성을 판단하기 위해, 진동대를 사용하여 강구조시험체의 진동실험을 행하고 결과적으로 얻어진 시험체의 수학적 모델에 대한 지진응답 수치해석결과와 진동실험에서의 관측기록을 비교하여 본 식별법의 타당성을 보인다.

포도 Muscat Bailey A의 安定生産을 위한 Gibberellin 處理試驗 (1)

裵基煥 진주산업대학교 1980 論文集 Vol.18 No.-

포도 Muscat Bailey A는 品種의 生産安定을 期하기 위하여, 이 地方에 알맞는 Gibberellin의 單回處理의 適期를 모색코저 100ppm. 濃度로써 滿開 直後(G_1區)와 滿開後 4日(G_2區), 7日(G^3區), 10日(G_4區), 13日의 處理區(G_5區)를 두어 그 處理가 果房에 미치는 影響을 調査하였던 바 다음과 같은 結果를 얻었다. 1. 熱期는 處理區가 無處理區에 비하여 1∼2日 程度 이르고, G_1區는 다른 處理區에 비하여 1日 정도 遲延되는 傾向이 있었다. 2. 果房長은 無處理區에 비하여 G_1, G_2區만이 길고, 其他의 處理區는 有意差가 없었다. 3. 果房重은 處理區가 모두 無處理區에 비하여 무겁고, 各 處理區間에는 有意差가 없었다. 4. 着粒數는 無處理區에 비하여 G_1區만이 많고, 其他의 處理區는 有意差가 없었다. 5. 平均果粒重은 G_1區를 除外하고는 各 處理區가 모두 無處理區에 비하여 무거웠고, 이들 各 處理區間에는 有意差가 없었다. 6. 果房內 4g 未滿의 小粒含有率은 無處理區에 비하여 G_1區는 현저히 높으나, 반대로 G^3, G_4, G_5區는 현저히 낮으며, G_2區는 有意差가 없었다. 7. 以上의 今年度의 試驗結果로 미루어서는 本品種에 대한 處理適期는 滿開後 7日부터 13日 사이에 있는 것으로 思料된다. In order to find the best time of gibberellin application for safe production of Muscat Bailey A grapes, this study was designed. GA_3 at 100 ppm concentration was applied by dipping the clusters in accordance with the designed treatments. The designed treatment were the following 5 kinds :G_1 treatment applied on the first day, G_2 treatment on the 4th day, G_3 treatment on the 7th day, G_4 treatment on the 10th day, G_5 treatment on the 13th day after full bloom. The results were as follows: 1. The maturities in all treatments were forced by one or two days compared with that in untreatment. 2. The cluster's lengths in G_1 and G_2 treatments were long, but those in the otder treatments were not different significantly, compared with that in untreatment. 3. The cluster's weights in all treatments were heavy compared with that in untreatment, but significant differences were not shown between those in these treatments. 4. The number of berries per cluster in G_1 treatment was largy, but those in the other treatments were not different significantly, compared with that in untreatment. 5. The mean weights of berry in the other treatmendts except G_1 treatment were heavy compared with that in untreatment, but significant differences were not shown between those in those treatments. 6. The percentage of light berries not exceeding 4gr. in G_1 treatment was remarkbly high, but on the contrary those in G_3, G_4 and G_5 treatments were remarkbly low, and that in G_2 treatment was not different significantly, compared with that in untreatment. 7. From the results of this study,I judged that the best time for gibberellin application was from the 7th day to 13th day after full bloom.