만성신부전환자에서 혈청 Adenosine Deaminase 활성도에 관한 연구

박용선(Yong Sun Park),김광선(Kwang Sun Kim),박용현(Yong Hyun Park),음선홍(Sun Hong Eum),조규웅(Kyoo Woong Cho),최기철(Ki Chul Choi),강영준(Young Joon Kang) 대한내과학회 1989 대한내과학회지 Vol.37 No.4

N/A To evaluate the clinical availability of serum adenosine deaminase (ADA) activity as an immunological parameter in chronic renal failure patients undergoing hemodialysis, serum ADA activities and other immunologica1 parameters, such as total lymphocyte count and its subpopulations and concentrations of immunoglobulin G, A and M, were measured simultaneously and analyzed for their correlations in 20 normal adults and 30 chronic renal failure patients. The results were as follows: 1) In normal adults, the mean value of serum ADA activities was 18.22±1.58U/L with no age and sex differences. 2) In two grous of long-term hemodialysis (over 12months) and short-term hemodialysis (under 1 month), mean values of serum ADA activities were 31.4±10.1U/ L and 33.6±12.3U/L respectively. There were significant differences between normal adults and the two hemodialysis groups, but there was no significant difference between the two hemodialysis groups. 3) There was no significant difference among various renal diseases. 4) Mean values of serum ADA activities decreased after hemodialysis in each group. 5) There were no significant correlations between serum ADA activity and BUN, creatinine, total lymphocyte count and subpopulations, and concentrations of immunoglobulin G, A and M. As mentioned above, serum ADA activities in chronic renal failure patients undergoing hemodialysis were higher than in normal adults, and there was a decreasing tendency of serum ADA activity immediately after hemodialysis, but serum ADA activity seems of little use as an immunological parameter in chronic renal failure patients.

단위가축영양 : 혼합생약제(고뿔(R))의 첨가가 육성돈의 성장, 면역관련 혈액학적 지표, 분내 휘발성 지방산과 암모니아태 질소 함량에 미치는 영향

조진호 ( J. H. Cho ),진영걸 ( Y. J. Chen ),민병준 ( B. J. Min ),김해진 ( H. J. Kim ),유종상 ( J. S. Yoo ),고태구 ( T. G. Ko ),현영 ( Y. Hyun ),김인호 ( I. H. Kim ) 한국동물자원과학회 ( 구 한국축산학회 ) 2006 한국축산학회지 Vol.48 No.3

Conjugated Linoleic Acid (CLA) 전구체 및 정제 부산물의 첨가가 산란계의 산란율, 계란 품질 및 면역 관련 혈액학적 지표에 미치는 영향

유종상,신승오,조진호,진영걸,김효진,황염,김영준,황광연,김정우,김인호,Yoo, J.S.,Shin, S.O.,Cho, J.H.,Chen, Y.J.,Kim, H.J.,Huang, Y.,Kim, Y.J.,Hwang, K.Y.,Kim, J.W.,Kim, I.H. 한국가금학회 2007 韓國家禽學會誌 Vol.34 No.3

This study was conducted to evaluate conjugated linoleic acid (CLA) precursor and produce supplementation on egg production, egg quality and blood immunological parameters in laying hens. The total of 252 (32-wk) Hy-line brown commercial hens were used for 5 weeks. Dietary treatments included 1) CON (basal diet + oat 1% + soybean oil 1%), 2) WOS (basal diet + whey 0.5% + oat 0.5% + soybean oil 1%), 3) WS (basal diet+ whey 1% + soybean oil 1%), 4) WOCLA (basal diet + whey 0.5% + oat 0.5% + CLA produce 1%), 5) WCLA (basal diet + whey 1% + CLA produce 1%) and 6) FOCLA (basal diet + Fish oil 0.5% + oat 1% + CLA produce 0.5%). For 4 weeks egg shell thickness was higher in WOS and FOCLA treatments than WOCLA and WCLA treatments (P<0.05). Egg weight was significantly increased (P<0.05) in WOS and FOCLA treatments compared to WS and WCLA treatments for 4 weeks. From 4 to 5 weeks yolk high, yolk color and haugh unit were significantly higher in FOCLA treatment than CON treatment (P<0.05). IgG concentration of blood was increased in WOS and FOCLA treatments compared to CON, WS and WOCLA treatments(P<0.05). In conclusion, fish oil, CLA produce and oat supplementation in laying hens diet improve egg shell thickness, yolk high, yolk color and haugh unit, also, CLA precursor supplementaion in laying hens diet improve IgG concentration of blood higher than CON treatment. 본 연구는 CLA 전구체 및 정제 부산물의 급여가 산란계의 산란율, 계란 품질 및 면역 관련 혈액학적 지표에 미치는 영향에 대하여 알아보기 위하여 시험을 실시하였다. 사양 시험은 32주령 ISA Brown 252수를 공시하였으며, 5주간 사양 시험을 실시하였다. 실험 설계는 1) CON(basal diet + oat 1% + soybean oil 1%), 2) WOS(basal diet + whey 0.5% + oat 0.5% + soybean oil 1%), 3) WS(basal diet+ whey 1% + soybean oil 1%), 4) WOCLA(basal diet + whey 0.5% + oat 0.5% + CLA produce 1%), 5) WCLA(basal diet + whey 1% + CLA produce 1%) 및 6) FOCLA(basal diet + Fish oil 0.5% + oat 1% + CLA produce 0.5%)로 6개 처리를 하여 처리당 7반복, 반복당 6수씩 완전 임의 배치하였다. 난각 두께는 시험 4주에서 WOS 및 FOCLA 처리구가 WOCLA 및 WCLA 처리구와 비교하여 유의적으로 높게 나타났으며(P<0.05), 난중은 시험 4주에서 WOS 및 FOCLA 처리구가 WS 및 WCLA 처리구와 비교하여 유의적으로 높게 나타났다(P<0.05). 난황고, 난황색 및 호우 유닛에 있어서 FOCLA 첨가구는 대조구와 비교하여 높게 나타났으며, 종료 시 IgG에 있어서 WOS 및 FOCLA 처리구가 대조구, WS 및 WOCLA 처리구와 비교하여 유의적으로 높게 나타났다(P<0.05). 결론적으로 어유, 정제 부산물 및 귀리의 첨가는 난각 두께, 난황고, 난황색 및 호우 유닛을 개선시키는 효과를 보여주었으며, CLA 전구체의 첨가 급여는 혈액 내 IgG의 함량을 대조구와 비교하여 증가시켰다.

수온 상승에 따른 북방전복, Haliotis discus hannai과 둥근전복, H. discus discus 체액의 생리학적 변화

민은영,이정식,김재원,전미애,강주찬,Min, Eun-Young,Lee, Jung Sick,Kim, Jae Won,Jeon, Mi Ae,Kang, Ju-Chan 한국패류학회 2015 The Korean Journal of Malacology Vol.31 No.1

본 연구에서는 수온 증가에 따른 북방전복 (Haliotis discus hannai) 과 둥근전복 (H. discus discus) hemolymph의 생리 및 면역학적 변화를 관찰하기 위하여 위 두 종을 20, 22, 24, 26 및 $28^{\circ}C$ 수온에 각각 4 일간 노출시켰다. 노출 결과, hemolymph의 total-protein (TP), glucose, 칼슘 (Ca) 은 둥근전복이 북방전복보다 높은 값을 보였으나, 마그네슘 (Mg), alkaline phosphatase (ALP) 및 lysozyme은 두 종에서 유사한 값을 보였다. 수온 증가에 따른 hemolymph의 TP, glucose 및 마그네슘은 두 전복 모두에서 유의한 변화가 관찰되지 않았다. 그러나 칼슘과 면역관련 인자인 ALP 및 lysozyme은 둥근전복에서 유의한 차이가 없었으나, 북방전복에서는 $26^{\circ}C$ 또는 $28^{\circ}C$의 고수온에 노출되었을 때, 유의하게 높은 활성을 보였다. 한편 phenoloxidase (PO)는 북방전복에서 높은 값을 보였으며, 수온이 높을수록 증가하는 경향을 보였다. 결론적으로 북방전복은 둥근전복과 달리 여러 지표에서 유의한 변화가 관찰된 것으로 보아 북방전복이 둥근전복보다 고수온에 보다 민감한 것으로 사료된다. This study was conducted to investigate the effects of elevated water temperature (WT) on biochemical and immunological factors in the hemolymph of the abalones, Haliotis discus hannai and H. discus discus. The abalone were exposed to various WT; 20, 22, 24, 26 and $28^{\circ}C$ for 4 days. In the control and $20^{\circ}C$, total-protein (TP), glucose and calcium (Ca) in hemolymph of H. discus discus were higher than the values in H. discus hannai. The values of magnesium (Mg), alkaline phosphatase (ALP) and lysozyme in H. discus hannai were similar to the H. discus discus in the control. There were no significant alterations in TP, glucose and Mg levels of hemolymph in H. discus hannai and H. discus discus by WT increases. The values of Ca, ALP and lysozyme were increased in H. discus hannai exposed to the high temperature (26 and $28^{\circ}C$) compared to control, while the values in H. discus discus were not significant difference between the WT groups. The phenoloxidase (PO) activity was increased in hemolymph of H. discus hannai exposed to high temperature (${\geq}24^{\circ}C$) compared to the control (P < 0.05). These physiological and immunological parameters were significantly changed in H. discus hannai. However, these parameters in H. discus discus were barely altered at the high WT (P < 0.05). These results suggested that H. discus hannai is considered to be more sensitive than H. discus discus at the high WT.

수온 증가에 따른 말전복, Haliotis gigantea과 둥근전복, Haliotis discus discus (Reeve, 1846) hemolymph의 생리학적 변화

민은영,김신후,황인기,김경욱,박보미,이정식,강주찬,Min, Eun-Young,Kim, Shin-Hu,Hwang, In-Ki,Kim, Kyeong-Wook,Park, Bo-Mi,Lee, Jung Sick,Kang, Ju-Chan 한국패류학회 2015 The Korean Journal of Malacology Vol.31 No.3

This study was conducted to examine the effects of alterations in water temperature (WT) on biochemical and immunological factors in the hemolymph of the abalones, Haliotis gigantea and H. discus discus. The abalone were exposed to various WT; 18, 20, 22, 24, 26 and $28^{\circ}C$ for 96 hours. In biochemical factors, total-protein (TP), glucose, magnesium (Mg), aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were not significant changes in hemolymph of H. gigantea and H. discus discus. But calcium was significantly increased by high WT (${\geq}24^{\circ}C$). In immunological factor, The phenoloxidase (PO) activity was decreased in hemolymph of H. gigantea and H. discus discus exposed to high temperature (${\geq}22^{\circ}C$) compared to the control (P < 0.05). Whereas alkaline phosphatase (ALP) was not significantly changed. These results suggested that high temperature adversely affects the immunity of H. gigantea and H. discus discus. 본 연구에서는 수온 증가에 따른 말전복 (Haliotis gigantea) 과 둥근전복 (H. discus discus) 헤모림프의 생리 및 면역학적 변화를 관찰하기 위하여 위 두 전복을 20, 22, 24, 26 및 $28^{\circ}C$ 수온에 각각 4 일간 노출시켰다. 노출 결과, 헤모림프의 totalo-protein (TP), glucose, calcium (Ca) 은 둥근전복 (H. discus discus)이 말전복 (H. gigantea) 보다 높은 값을 보였으나, magnesium (Mg), alkaline phosphatase (ALP) 두 전복에서 유사한 값을 보였다. 그리고 면역인자인 PO에서는 둥근전복 (H. discus discus)이 더 높게 나타났다. 수온 증가에 따른 헤모림프의 TP, glucose, Mg, AST, ALT 및 ALP는 유의적인 변화가 나타나지 않았다. 하지만 헤모림프의 Ca 농도는 둥근전복 (H. discus discus)에서 $24^{\circ}C$ 이상, 말전복 (H. gigantea)에서는 $26^{\circ}C$ 이상의 수온에서 유의적 증가를 보였다. 그리고 면역인자인 phenoloxidase (PO) 는 둥근전복 (H. discus discus)은 $22^{\circ}C$ 이상 말전복 (H. gigantea) 에서는 $26^{\circ}C$ 이상의 수온에서 유의적 감소를 나타내었다. 결론적으로 말전복 (H. gigantea)은 $24^{\circ}C$ 이상에서 혈액학적 변화를 보였고, $26^{\circ}C$ 이상에서 면역학적 변화를 보였다. 둥근전복 (H. discus discus)은 $26^{\circ}C$ 이상에서 혈액학적 변화를 보였고, $22^{\circ}C$ 이상에서 면역학적 변화를 나타내었다.

수온 증가에 따른 말전복, Nordotis gigantea과 둥근전복, Haliotis discus discus (Reeve, 1846) hemolymph의 생리학적 변화

민은영,김신후,황인기,김경욱,박보미,이정식,강주찬 한국패류학회 2015 The Korean Journal of Malacology Vol.31 No.3

This study was conducted to examine the effects of alterations in water temperature (WT) on biochemical and immunological factors in the hemolymph of the abalones, Nordotis gigantea and Haliotis discus discus. The abalone were exposed to various WT; 18, 20, 22, 24, 26 and 28℃ for 96 hours. In biochemical factors, total-protein (TP), glucose, magnesium (Mg), aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were not significant changes in hemolymph of N. gigantea and H. discus. But calcium was significantly increased by high WT (≥ 24℃). In immunological factor, The phenoloxidase (PO) activity was decreased in hemolymph of N. gigantea and H.D. hannai exposed to high temperature (≥ 22℃) compared to the control (P < 0.05). Whereas alkaline phosphatase (ALP) was not significantly changed. These results suggested that high temperature adversely affects the immunity of N. gigantea and H.D. hannai.

수온 증가에 따른 북방전복, Haliotis discus hannai과 둥근전복, Haliotis discus discus의 체액의 생리학적 변화

민은영,이정식,김재원,전미애,강주찬 한국패류학회 2015 The Korean Journal of Malacology Vol.31 No.1

This study was conducted to investigate the effects of elevated water temperature (WT) on biochemical and immunological factors in the hemolymph of the abalones, Haliotis discus hannai and Haliotis discus discus. The abalone were exposed to various WT; 20, 22, 24, 26 and 28℃ for 4 days. In the control, 20℃, total-protein (TP), glucose and calcium (Ca) in hemolymph of H. discus discus were higher than the values in H. discus hannai. The values of manesium (Mg), alkaline phosphatase (ALP) and lysozyme in H. discus hannai were similar to the H. discus discus in the control. There were no significant alterations in TP, glucose, and Mg levels of hemolymph in H. discus hannai and H. discus discus by WT increases. The values of Ca, ALP, and lysozyme were increased in H. discus hannai exposed to the high temperature (26 and 28℃) compared to control, while the values in H. discus discus were not significant difference between the WT groups. The phenoloxidase (PO) activity was increased in hemolymph of H. discus hannai exposed to high temperature (≥ 24℃) compared to the control (P < 0.05). These physiological and immunological parameters were significantly changed in H. discus hannai. However, these parameters in H. discus discus were barely altered at the high WT (P < 0.05). These results suggested that H. discus hannai is considered to be more sensitive than H.D. discus at the high WT.

고수온 스트레스에 대한 둥근전복, Haliotis discus discus (Reeve, 1846), 말전복, Haliotis gigantea의 항산화 및 면역학적 반응

민은영(EunYoung Min),김신후(Shin Hu Kim),이정식(Jung Sick Lee),강주찬(Ju-Chan Kang) 한국패류학회 2017 The Korean Journal of Malacology Vol.33 No.2

A study was conducted to evaluate the effects of elevated water temperature on antioxidant and immunological response in the abalones, Haliotis discus discus and Haliotis gigantea. The abalones were exposed to various water temperature; 20, 21, 22, 24, 26 and 28℃ for 96 hours. After thermal stress for 96 hours, in the control, superoxide dismutase (SOD) activities in hepatopancreas of H. gigantea were higher (1.3 times) than the values in H. discus discus. Although SOD activities showed no significant differences in H. discus discus, the values in H. gigantea were significantly increased in 24℃ or higher compared to the control after 24 hours exposure. Catalase (CAT) activities in hepatopancreas of H. discus discus were higher (1.4 times) than the values of H. gigantea in the control. After thermal stress (≥ 26℃), CAT activities significantly increased in both species compared to the control. The phenoloxidase (PO) activity of hemolymph was decreased in both species exposed to high temperature (≥ 26℃) compared to the control (P < 0.05). The values of lysozyme were significantly decreased in H. discus discus exposed to 28℃, and in H. gigantea to 26℃. In conclusion, in both species, there was a significant increase on antioxidant abilities (CAT) and a decrease on immunological parameters (PO and lysozyme) after thermal stress of 26℃ or higher. Also, antioxidant enzymes, SOD activities significantly increased in H. gigantea, but there was no significance in H. discus discus after thermal stress. These results suggested that H. gigantea is considered to be more sensitive than H. discus discus on thermal stress even though the similar warm current type.

Innate immune response and disease resistance in Carassius auratus by triherbal solvent extracts

Harikrishnan, R.,Balasundaram, C.,Kim, M.C.,Kim, J.S.,Han, Y.J.,Heo, M.S. Academic Press 2009 FISH AND SHELLFISH IMMUNOLOGY Vol.27 No.3

This study reports the effect of aqueous, ethanol and methanol triherbal solvent extract from Azadirachta indica, Ocimum sanctum and Curcuma longa on innate immune mechanisms such as phagocytosis activity, respiratory burst activity, alternative complement activity and lysozyme activity and disease resistance in goldfish (Carassius auratus) against Aeromonas hydrophila. Fish were intraperitoneally injected with different doses of 0, 5, 50 and 100 mg kg<SUP>-1</SUP> body weight of each triherbal solvent extracts. The functional immunity in terms of percentage mortality and Relative Percent Survival (RPS) and innate immune response was assessed on week 1, 2 and 4 by challenging with live A. hydrophila (1 x 10<SUP>7</SUP> cells ml<SUP>-1</SUP>). All the chosen innate immune parameters were enhanced in the ethanol and methanol triherbal solvent extract treatment after week 2. However, the aqueous triherbal extract was enhanced only after week 4. The ethanol and methanol triherbal solvent extracts administration preceding the challenge with live A. hydrophila decreased the percentage mortality in the experimental groups with the consequence increase in RPS values. The study indicates that all the doses of ethanol or methanol triberbal solvent extracts could be positively influence the immune response and protect the heath status of goldfish against A. hydrophila infection.

유기산 복합물 급여가 육계 생산성, 장기무게, 혈액내 면역적 성상 및 장내 융모 형태에 미치는 영향

장해동(H. D. Jang),유종상(J. S. Yoo),김효진(H. J. Kim),신승오(S. O. Shin),황염(Y. Hwang),주천상(T. X. Zhou),진영걸(Y. J. Chen),조진호(J. H. Cho),김인호(I. H. Kim) 한국가금학회 2008 韓國家禽學會誌 Vol.35 No.1