당원, 삼투압 및 무기염 농도가 Panax ginseng 모상근의 생장과 Ginsenosides 생산성에 미치는 영향

한영현,임종훈,백기엽,유기원 충북대학교 한국과학재단 지정 첨단원예기술개발 연구센터 1998 연구보고서 Vol.3 No.-

인삼 형질전환체는 호르몬이 포함되지 않은 3/4SH 배지에 공구유출식 콘형 생물반응기를 이용하여 배양하였다. 초기 접종 량은 30g/3L로 하였으며 4-5일 간격으로 배양액 내의 당원, 전기 전도도와 pH의 변화를 측정하였다. 처음 pH는 5.8이었으나 배양8일 후 4.95까지 떨어졌으나, 이후 5.63까지 증가하였다. 전기전도도와 탄소원으로 제공된 sucrose는 일정하게 감소되었고, 특히 배양 미삼의 탄소원으로 제공된 sucrose는 배양 20일 후에 완전히 소모되었다. 분해산물인 glucose와 frutose는 20일 까지 점진적으로 증가되었고 이후 급격히 감소되었다. 400ml 플라스크 안에 100ml의 배지량을 첨가하고 3g의 배양 미삼을 접종하여 배양하였을 때 배양 미삼의 생장률은 탄소원의 농도에 따라 증가되었다. 이는 배양 미삼의 환원당, 전당, 그리고 전분의 저장함량이 증가되었다. 그러나 ginsenosides의 함량은 반 비례관계로 감소되었다. 배양 주간별 ginsenosides의 함량변화는 배양초기인 1,2 주에는 급격히 감소되었으며 3주에는 약간 증가하고 4,5주 사이에 급격히 증가되었다. 삼투조절제로 사용한 0.1, 0.2, 0.3M의 mannitol과 sobitol은 생장 면에서 0.1M의 실험구가 좋았으나 ginsenosides의 함량은 삼투압농도와 반비례 관계로 급격히 감소되었다. Panax ginseng roots transformed by Agrobacterium rhizogenes were cultured in the air-lifted cone type bubble bioreactor that contained a hormone-free SH medium with 3% sucrose. The inoculating vol. was 30g/3L. The pH, EC, glucose, fructose and sucrose were investigated every 4 or 5 days. The pH was 5.8 initially and become 4.95 after 8 days and then increased after to 5.63. EC and sucrose contents decreased with culture days and then especially sucrose, which is carbon source for bio-ginseng rots, were depleted after 20 days. Fructose and glucose content increased gradually till 20 days with regular intervals, and then decreased quickly. While the roots were culture in 400ml flask, the higher sucrose consents, the higher growth rate. The growth rate and starch, reducing and total sugar contents were increased with the increase of sucrose concenturations but the contents of ginsenoside decrease. Variations of ginsenosides each weeks decreased first 1 or 2 weeks, increase a little after 3 weeks and largely between 4 and 5 weeks of flask culture. The osmotic regulation regents on growth were treated the 0.1, 0.3, 0.5M sobitol and mannitol. Amount of the growth were all increased on 0.1M but largely decreased the ginsenoside.

클렌부테롤의 피부투과에 미치는 경피흡수촉진제의 영향

최한곤,이종달,유봉규,용철순 한국약제학회 2003 Journal of Pharmaceutical Investigation Vol.33 No.1

Clenbuterol, a selective β_2-adrenergic receptor stimulant, has been introduced as a potent bronchodilator for patients with bronchial asthma, chronic obstructive bronchial disease, chronic bronchitis and pulmonary emphysema. The percutaneous permeation of clenbuterol was investigate in hairless mouse skin after application of 50/50 buffer(pH 10)/propylene glycol solvent mixture. The enhancing effects of various penetration enhancers such as terpenes, non-ionic surfactants, pyrrolidones, fatty acids and some other enhancers on the permeation of clenbuterol were evaluated using Franz diffusion cell. Among terpenes studied, 1,8-cineole was the most effective enhancer, which increase the permeability of clenbuterol approximately 39.33-fold compared with the control without penetration enhancer, followed by menthone with enhancement ratio of 23.57. Nonionic surfactants did not have significant enhancing effects. N-Lauryl-2-pyrrolidone increased the permeability of clenbuterol approximately 4.51-fold compared with the control. Lauric acid increased the permeability of clenbuterol approximately 35.57-fold with decreasing the lag time from 2.64 to 0.52 hr. Oleic acid, linoleic acid, the permeability of clenbuterol 9.24-fold compared with that without enhancer.

다시마 식이가 흰쥐에서 글리피지드의 체내동태에 미치는 영향

최한곤,장보현,이종달,유봉규,용철순 한국약제학회 2003 Journal of Pharmaceutical Investigation Vol.33 No.2

Drug interactions with food, on occasion, lead to serious nutritional and functional changes in the body as well as alternations of pharmacological effect. It, therefore, should be necessary to take drug interactions with food into consideration for effective and safe therapeutics. Diabetes mellitus is a heterogeneous group of disorders characterized by abnormal glucose homeostasis, resulting in hyperglycemia, and is associated with increase risk of micovascular, macrovascurlar, and neuropathic complications. However, the precise mechanixm of diabetes mellitus remains unclear. Three basic objectives in the care of diabetic patients are maintaining optimal nutrition, avoiding hypo- or hyperglycemia and preventing complications. The purpose of this study was to investigate the effect of Laminaria japonica diet on the absorption, distribution, metabolism and excretion of glipizide which are frequently used in the treatment of diabetes. Diabetic rats induced by streptozotocin were employed in this study. Blood concentrations of oral hypoglycemic agents were measured by HPLC and resultant pharmacokinetic parameters were calculated by RSTRIP. The mechanisms of drug interaction with food were evaluated on the basis of pharmacokinetic parameters such as k_a, t_1/2, C_max, t_max, and AUC. Administration of glipzide in normal rats treated with Laminaria japonica diet showed significant increase in AUC, k_a, t_1/2, t_max and decrease in C_max, compared to those without Laminaria japonica diet. This might result from adsorption of glipizide on components of Laminaria japonica, causing delayed absorption. Administration of glipizide in diabetic rats treated with Laminaria japonica diet showed significant increase in t_1/2 and t_max, and decrease in C_max, compared to those without Laminaria japonica diet. This might also result from adsorption of glipizide on components of Laminaria japonica, causing delayed absorption and flattened blood concentration of glipizide. The oral glucose test showed that Laminaria japonica diet could lower blood glucose level probably through either inhibiting the activity of disaccharidases, intestinal digestive enzymes, or delaying the absorption of glucose. More studies should be followed to fully understand pharmacokinetic changes of glipizide caused by long-term Laminaria japonica diet.

흰쥐에서 다시마 식이가 메트폴민의 체내동태 및 당 흡수에 미치는 영향

최한곤,장보현,이종달,김정애,유봉규,용철순 한국약제학회 2003 Journal of Pharmaceutical Investigation Vol.33 No.3

Drug interactions with food, on occasion, led to serious nutritional and functional changes in the body as well as alterations of pharmacological effect. It, therefore, should be necessary to take drug interactions with food into consideration for effective and safe therapeutics. Diabetes mellitus is a heterogeneous group of disorders characterized by abnormal glucose homeostasis, resulting in hyperglycemia, and is associated with increased risk of microvascular, macrovascular, and neuropathic complications. However, the precise mechanism of diabetes mellitus remins unclear. Three basic objectives in the care of diabetic patients are maintaining optimal nutrition, avoiding hypo-or hyperglycemia and preventing complications. Laminaria japonica is a brown macroalgae which can be used as a functional diet due to high content of diatery fiber. the purpose of this study was to investigate the effect of Laminaria japonica diet on the pharmacokinetics of metformin which are frequently used in the treatment of diabetes. Diabetic rats induced by strep-tozotocin were employed in this study. Blood concentrations of oral hypoglycemic agent, metformin, were measured by HPLC and resultant pharmacokinetic parameters were calculated by RSTRIP. The mechanisms of drug interaction with food were evaluated on the basis of pharmacokinetic parameters such as k_(a), t_(1/2), C_(max), t_(max), and AUC, C_(max), and k_(a), and increase in t_(max), compared to those with normal diet. This oral glucose test showed that Laminaria japonica diet could lower blood glucose level probably through either inhibiting the activity of disaccharidases, intestinal digestive enzymes, or delaying the absorption of glucose. More studies should be followed to fully understand pharmacokinetic changes of metformin caused by ong-term Laminaria japonica diet.

광양만의 저질 중금속 농도 분포 특성

김도희,유한홍,정종성,조현서,신현출 木浦海洋大學校 2002 論文集 Vol.10 No.1

The main goals of the study were to investigate the sediment quality and trace metal distribution in sediment of Kwangyang Bay, south coastal of Korea. Surface sediment sampling were performed in November, 2000 and February and March, 2001. The concentration of H₂S, IL and COD in sediment were measured by a manual of the Department of Marine Environment of Korea. The extracted trace metals Fe, Cu, Cd, Pb, and Zn in sediments mean analyzed by the flame atomic absorption spectrophotometer with an instrument of model Shimadzu AA-6701F. H₂S, IL and COD in the surface sediment were 1.3 mg/g, 5.9 % and 15.0 mg/g, respectively. The mean content of Fe, Cu, Cd, Pb and Zn were 1523 mg/kg, 4.799 mg/kg, 1.321 mg/kg 9.285 mg/kg and 9.353 mg/kg, respectively. All of these measured trace metals level were over the other reported trace metal level in sediment of coastal area. According to histograms, the content of H₂S, IL and COD and trace metals in sediment of this study were affected by artificial activities rather than natural process.

프로구단 웹사이트를 통한 6C 전략 구축방안 연구

이사겸,이성주,유한종 한국스포츠리서치 2003 한국 스포츠 리서치 Vol.14 No.6

This study aims to provide better service to customers and to work out marketing strategies for making 6C(contents, communication, commerce, community, connection, customization), through comparing and analyzing official web site of the domestic professional sports teams. The web sites should develop various good quality of information and to increase the user's satisfaction. The web sites of satisfaction to increase your teams loyalty. The loyalty is very important things for business and operating teams. And then, the develop of web sites for user. However tired you may be, you must it. Sometimes user conflicted of the web site which not enough contents, commerce so on. There are mistaken. Not that sports teams disliked the operated web sites, but that sports teams no cognition for web site. This study to meet the purpose, was made through investigated of books and official web sites. The objective of this research is for you to click the internet order site 'www.rankey.com' for high position the sports web sites. And more, using the other article. As a result, the web sites of the sports teams have a lack of contents and commerce, community, however, construction of shopping mall with low cost and new technological development. Firstly, users very important to communication in quickly response to BBS, Q&A. And then, user to solved of his question and trouble, conflict. Secondly, the web sites develop the contents for user and customers of enjoy. Thirdly, the web sites include of community for customization and communication with marketing targets. Fourthly, in this study, the satisfaction of sports web sites develop on game informations for users. The user wanted to information of player news and game news for enjoyed himself. So many people wanted to different of other web sites and contents. Finally suggestion, connection of the other web sites for sports teams have a lack contents and information.

도시 노숙자의 주요 정신장애에 관한 역학 연구

안준호,홍진표,유제춘,박종익,이철,한오수 大韓神經精神醫學會 2001 신경정신의학 Vol.40 No.2

외환위기 이후 대도시를 중심으로 노숙자들이 급증하여 이들이 심각한 사회문제로 등장하였다. 외국의 연구에 의하면 노숙자 중 상당수가 만성 정신질환을 앓고 있으며, 정신질환이 노숙의 원인 및 예후와 밀접한 관련이 있다고 한다. 그러나 국내에서는 아직 노숙자의 정신장애의 유병률에 관하여 알려진 바 없으므로 이에 대하여 조사하였다. 방법: 1998년 말부터 1999년 초까지 부산지역의 쉼터 두 곳에 거주하는 노숙자 216명을 대상으로 인구학적 자료에 대한 설문조사를 한 뒤 Structured Clinical Interview for DSM-Ⅳ(SCID)을 이용하여 Axis Ⅰ의 주요 정신장애를 진단하였다. 이들의 정신장애의 평생유병률 및 현재유병률을 국내 일반인구 및 외국 노숙자의 정신장애의 유병률과 비교하였다. 결과: DSM-Ⅳ Axis Ⅰ의 정신장애 중 기분장애, 정신병적 장애, 물질사용장애에 대한 진단 평가 결과 상기 정신장애의 전체 평생유병률은 60.1%, 현재유병률은 35.7%이었다. 그 중 주요우울장애의 평생유병률 이 22.5%로서 외국의 노숙자에 대한 역학연구 결과와 비교해 볼 때 높은 수준이었고 알코올 사용 장애의 평생유병률은 47.4%이었다. 정신병적 장애의 평생유병률은 3.8%이었고, 그 중에서도 정신분열병의 평생유병률은 1.9%이어서 외국의 노숙자에 대한 연구결과보다 낮은 편이었다. 결론: 국내 노숙자에서 기분장애와 물질사용장애가 주된 정신장애였으며 정신분열병 등 정신병적 장애의 유병률은 외국에 비해 낮은편이었다. 이러한 정신장애를 가지고 있는 노숙자에 대하여 접근하고 치료하기 위한 대책이 마련되어야 할 것이다. Objective:As homeless people increased in urban areas after the economic crisis in Korea, they became a serious social problem. Foreign research showed that many homeless people had chronic mental illnesses which were closely related with the cause and prognosis of homelessness. But very little was known about the prevalence of mental disorders in Korean homeless people. This study tried to capture the overall picture of mental disorders in homeless people. Method:The study subjects were 216 homeless people who stayed at two Pusan shelters between late 1998 and early 1999. Questionnaires on sociodemographic data were administered to the subjects, and then diagnoses of major DSM-IV Axis I mental disorders were made using Structured Clinical Interview for DSM-IV Axis I Disorders(SCID). The lifetime and current prevalence of mental disorders in this study were compared to those from other studies in Korean general population and in foreign homeless people. Results:The lifetime prevalence of major DSM-IV mental disorders(mood disorders, psy-chotic disorders, and substance use disorders) of 216 homeless people was 60.1%, and current prevalence was 35.7%. The lifetime prevalence of major depressive disorders was 22.5% which was higher than that of foreign studies. The lifetime prevalence of alcohol use disorder was 47. 4%. The lifetime prevalence of psychotic disorders was 3.8%, and among them the lifetime prevalence of schizophrenia was 1.9% which was lower than that of foreign studies. Conclusions: In Korean homeless people, mood disorders and substance use disorders were the main mental disorders and psychotic disorders such as schizophrenia were less than in foreign homeless people. The plans to approach and treat homeless people with mental disorders should be made.

폴록사머 및 프로필렌글리콜을 이용한 클로트리마졸 고형 좌제의 물리화학적 특성

현경희,오유경,김정애,공경환,김지현,양준호,배명수,김호동,이종달,장현욱,용철순,최한곤 한국약제학회 2005 Journal of Pharmaceutical Investigation Vol.35 No.2

To develop a clotrimazole-loaded solid suppository with poloxamer and propylene glycol, the melting points of various formulations composed of poloxamer 188 (P 188) and propylene glycol were investigated. The dissolution study of clotrimazole delivered by the suppository composed of P 188 and propylene glycol was performed. The mixtures composed of P 188 and propylene glycol were homogeneous. Propylene glycol affected the melting points of poloxamer mixtures. In particular, the mixture [P 188/propylene glycol (70/30%)] with the melting point of about 32°C was a solid form at room temperature and instantly melted at physiological temperature. Furthermore, propylene glycol affected greatly the dissolution rates of clotrimazole from the suppository. Dissolution mechanism analysis showed the dissolution of clotrimazole was proportional to the time. Our results indicated that the solid suppository with P 188 and propylene glycol would be a candidate of rectal dosage form for clotrimazole.

폴록사머를 이용한 디클로페낙 고형 좌제의 개발

용철순,오유경,김정애,김용일,박상만,양준호,이종달,최한곤 한국약제학회 2004 Journal of Pharmaceutical Investigation Vol.34 No.2

To develop a poloxamer-based solid suppository with poloxamer mixtures, the melting points of various formulations composed of P 124 and P 188 were investigated. To investigate the effect of poloxamer to the dissolution and dissolution mechanism of diclofenac sodium from the suppository the dissolution of diclofenac sodium delivered by the poloxamer-based suppository was performed. Furthermore, to investigate the mucoadhesive property of the poloxamer-based solid suppository, the identification test in the rectum was carried out after its rectal administration in rats. The poloxamer mixtures composed of P 124 and P 188 were homogeneous. Very small amounts of P 188 affected the melting points of poloxamer mixtures. In particular, the poloxamer mixture [P 124/P 188 (97/3%)] with the melting point of about 32℃ was a solid form at room temperature and instantly melted at physiological temperature. Furthermore, very small amounts of P 188 in the poloxamer-based suppository hardly affected the dissolution rates of diclofenac sodium from the suppository. Dissolution mechanism analysis showed the dissolution of diclofenac sodium was proportional to the time. At 4 h after administration, the blue color of poloxamer-based suppository [diclofenac sodium/poloxamer mixture (2.5/97.5%)] with the P 124/P 188 ratio of (97/3%) and blue lake in the rectum was faded. However, the position of suppository in the rectum did not significantly change with time. Thus, it retained in the rectum for at least 4 h. Our results indicated that the poloxamer-based solid suppository with P 124 and P 188 would be a candidate of rectal dosage from for diclofenac sodium.

엄지발가락에 발생한 건초 섬유종 1예

이경호,조유경,한예원,김진아,김형민,박철종 대한피부과학회 2006 大韓皮膚科學會誌 Vol.44 No.4