생약재 첨가 사료를 투여한 넙치(Paralichthys olivaceus)의 비특이적 면역반응, 혈액성분 및 항병력 효과

정승희,이주석,한형균,전창영,이해영 한국어병학회 2002 한국어병학회지 Vol.15 No.1

본 연구는 식물성 생약재의 열수추출이 농도별로 첨가된 사료를 넙치(Paralichthys olivaceus)에 경구 투여한 다음 비특이적 면역반응 및 질병 저항성의 향상여부를 알아보고자 하였다. 실험에는 약쑥과 삼지구엽초를 7:3(w/w)의 비율로 열추출한 후, 0%, 0.1%, 0.5% 및 1%의 농도로 첨가하여 만든 2종류 혼합생약재(2-혼합구)와 약쑥, 삼지구엽초, 연교, 구절초, 박하, 지유, 삼백초, 상백피, 팔각의 9종류 생약재가 동일한 농도로 열수추출한 후 제작한 9종류 혼합생약재(9-혼합구)를 사용하였다. 이들 농도별 첨가사료를 넙치(평균 10.3±2.5g)에 12주 동안 경구 투여하면서 혈청내 라이소자임 활성, 혈청의 Escherichia coli에 ??살균능력 및 혈액학적 변화를 조사하였다. 그리고 최종 투여 12주 후에 넙치 에드와드병의 원인균 Edwardsiella tarda의 생균 공격실험을 실시하여 상대생존율(RPS)로써 저항성 향상효과를 살펴보았다. 2-혼합구 0.1%는 비특이적 면역활성을 크게 증가시켰으며, 상대생존율이 67%로 높게 나타나 가장 효과적인 질병 저항성 향상효과를 보였다. 따라서 2-혼합구 0.1%는 넙치 체내 비특이적인 면역기구를 자극함으로써 어체의 자연저향성을 증강시켜 세균성 질병의 감염에 대한 방어력을 상당히 향상시킬 수 있을 것으로 사료된다. 9-혼합구 0.1% 및 0.5%는 비록 비특이적 면역활성은 증가시키지 못하였으나, 상대생존율은 각각 53%를 나타내어 질병 저항성을 일부 향상시키는 효과가 있었다. Effects of medicinal herb extract on nonspecific immune responses, hematology and disease resistance against Edwardsiella tarda in olive flounder, Paralichthys olivaceus were evaluated. Wormwood, Artemisia asiatica NAKAI and barrenwort, Epimedium koreanum NAKAI were mixed at a ratio of 7 : 3 (w/w) for 2-herbs extract and wormwood, barrenwort, Korean forsythia, Forsythia koreana NAKAI, chrysanthemum, Chrysanthemum zawadskii var. latilobum KITAMURA, peppermint, Mentha arvensis L. var, piperascens MALINV., great burnet, Snaguisorba afficinalis L., Lizard tail. Saururus chinensis BAILL., mulberry, Morus alba L., and star anise, Illicium varum HOOK, f, at the same weight for 9-herbs extract. Two-herbs of 9-herbs extract were prepared by heating after adding 10㎖ of distilled water per g of the herb mixtures. Fish (10.3±2.5g) were fed the experimental diets supplemented with the 2-herbs or 9-herbs extract at the different concentrations of 0%, 0.1%, 0.5% and 1% per kg diet for 12 weeks. Lysozyme and bactericidal activities of serum, and hematological characteristics were examined during experimental period. After feeding test period, all experimental groups were challenged with E. tarda. Lysozyme activity from the fish fed the diet supplemented with 0.1% or 0.5% of 2-herbs extract was significantly higher than the control. But there was no difference both in bactericidal activity and hematology among each group. Sixty seven % of relative percent survival values (RPS) in the group fed the diet supplemented with 0.1% of 2-herbs was higher than the other group and the control. These results suggest that supplenmentation of 0.1% of 2-herbs extract to a commercial diet may enhance disease resistance in olive flounder. Although both 0.1% and 0.5% 9-herbs extract did not improve non-specific immune reponses, they could enhance disease resistance of 53% RPS, respectively.

土壞 및 植物體 중 窒酸態 窒素 簡易測定 方法 探索

鄭英祥,梁在義,韓連奎,崔文憲 江原大學校 附設 環境硏究所 1996 環境硏究 Vol.13 No.-

Feasibility of simple rapid methods to determine nitrate concentration in soil and plant sap in siltu was assessed using Merckoquant strip method and Cardy NO_(3)- sensor. The saturated extract of a soil was sampled readily in field by a simple apparatus which consists of a syringe, a vial and a hand vacuum pump with a vacuum valve. The simple measurement methods for saturated extract were tested for soil samples obtained from the thirty three farm vinyl houses around Chuncheon, Kang-weon-Do and Kuri, Kyeonggi-Do. The methods were tested for soil and plant sap samples grown under different fertilization in a vinyl house at Kangweon National University. The main treatments were with or without organic fertilizer, and subtreatments were four nitrogen levels ; 0, 100, 200 and 400kg/ha for lettuce and 0, 150, 300 and 500k9/ha for cucumber. The Cardy sensor method showed a high confidence for soil and plant sap nitrate measurement. The Merckoquant strip method was also reliable, but checking electrical conductivity was recommended for better reliability. The soil nitrate concentration above 10mmo1/L was not desirable for lettuce yield, and 15mmo1/L for cucumber. The respective electrical conductivities of saturated extracts were 3 to 4ds/m at these concentrations. The soil nitrate concentration could be monitored by Amberlite IRN-150 Resin-bag. A good correlation was obtained between the soil nitrate concentration and the nitrate absorbed by the resin bag. Therefore, use of the resin bag could be recommended to monitor nitrate in soil, minimizing soil destruction.

축구선수의 Detraining과 Retraining이 혈청지질 및 호르몬농도에 미치는 영향

정정화,박재현,채종훈,성혜련,황지인,윤미숙,노금선,윤종관,윤영학,노순덕,정경숙,박일규,김은희,박현태,박상갑 대한스포츠의학회 1999 대한스포츠의학회지 Vol.17 No.1

The purpose of this study was to investigate the effects of detraining and retraining on serum lipid and hormones in soccer players. Subjects were seven male high-school soccer players. V˙O_2max was determined for each subjects by administering a treadmill test(initial speed: 90m/min, grade: 5%, increasing speed per 3 min: 30m/min). Serum lipid(T-C, TG, HDL-C LDL-C) and hormones(epinephrine, norepinephrine, growth hormones, cortisol) were assayed pre and post detraining in 10, 20, 30 days after retraining. The repeated ANOVA was used to determine significant differences. The 0.05 level of significance was as critical level for the study. The results of the study were as follows: 1. V˙O_2max(ml/min) were 3576.3±204.2ml/min pre detraining, 3234.1±198.9 ml/min post detraining. There are significant(p<.05) difference between pre and post detraining. In 10, 20, 30 days after retraining, V˙O_2max(ml/min) were 3601.4±170.9 ml/min. There were significantly(p<.05) increased in retraining periods. 2. V˙O_2max(ml/kg/min) were significantly(p<.05) decreased from 62.3±2.9 ml/kg/min to 55.9±4.7 ml/kg/min in detraining. In 10, 20, 30 days after retraining, V˙O_2max(ml/kg/min) were 62.4±3.4ml/kg/min, 62.7±2.3ml.kg/min, 67.3±7.2ml/kg/min respectively. There were significantly(p<.05) increased in retraining periods. 3. T-C were significantly (p<.05) increased from 166.6±8.5mg/dl to 175.3±10.3 mg/dl in detraining. In 10, 20, 30 days after retraining, T-C were 160.1± 3.2mg/dl, 156.7±3.7mg/dl, 140.3±9.0mg/dl. There were significantly(p<.05) decreased in retraining periods. 4. HDL-C were 61.4±6.6mg/di pre detraining, 5.3±6.6mg/dl post detraining. There are significant(p<.05) difference between pre and post detraining. In 10, 20, 30 days after retraining, HDL-C were 56.9±7.1mg/dl, 56.4±9.2mg/dl, 57.7±9.1mg/dl respectively. There were no significant difference in retraining periods. 5. The hormones(epinephrine. norepinephrine, growth hormone, cortisol) were changed as same patterns. Epinephrine were 26.0±7.0[g/ml pre detraining, 24.6±3.2pg/ml post detraining. In 10, 20, 30 days after retraining, epinephrine were 26.9±5.6pg/ml, 30.6±6.2pg/ml, 29.4±5.6pg/ml respectively. There were no significant difference in retraining periods. In conclusion, HDL-C, epinephrine, norepinephrine, growth hormone and cortisol were decreased, T-C, LDL-C and TG were increased in detraining. But HDL-C, epinephrine, norepinephrine, growth hormone and cortisol were increased, T-C, LDL-C and TG were decreased in retraining.

DESMOPLASTIC AMELOBLASTOMA의 치험 1례

김성국,신홍인,박희경,장현중,김영미,김진수 大韓顎顔面成形再建外科學會 1996 Maxillofacial Plastic Reconstructive Surgery Vol.18 No.1

Ameloblastomas are benign epithelial neoplasm of the jaw comprising approximately 1-3.3% of all odontogenic cysts and tumors. Although most are microscopically benign, they are generally considered to be locally aggressive and destructive, exhibiting a high rate of recurrence. Treatments of them contain the conservative treatments on unilocular types and radical treatments on multilocular types. Classifications based on the histologic features of ameloblastoma generally included the follicular, plexiform, acanthomatous, basal cell, uncystic types. However, recantly a new and unusual variant has been added by Eversole et. al in 1984, the histologic features of which are characterized by marked stromal desmoplastic proliferation. This is a case report of desmoplastic ameloblastoma in the anterior maxilla of 63 year-old female treated by block excision. THis provides a brief review of the literature because of the rarity and unusual radiographic-histologic features of desmoplastic ameloblastoma.

유구치 상아질의 각 부위에 적용된 수종의 복합레진 접착제의 미세인장접착강도에 관한 연구

유정은,최영철,최성철,박재홍 大韓小兒齒科學會 2009 大韓小兒齒科學會誌 Vol.36 No.3

본 연구는 유구치 상아질의 각기 다른 부위(표층부, 심층부, 치경부)에서 복합레진 접착제의 접착강도를 비교, 평가하기 위하여 임상에서 흔히 사용하고 있는 서로 다른 4종의 접착방식(3-step total etch: 1군, 2-step total etch: 2군, 2 step self-etch: 3군, all-in-one: 4군)을 적용하고 복합 레진(Light-Core™ Core Build-Up Composite)을 적층한 후, 미세인장접착강도를 비교하였을 때 다음과 같은 결과를 얻었다. 1. 접착방식간의 비교에서 레진의 접착방식에 따른 상아질 각 부위에서의 미세인장접착강도는 제 1군이 표층부에서 뚜렷이 높았으나(p<0.05) 2, 3, 4군은 부위에 따른 차이가 없었다. 2. 부위별 미세인장접착강도를 비교하여 보았을 때 상아질 표층부에서의 미세인장접착강도는 레진의 접착 방식간에 차이가 없었으나, 심층부에서는 2군, 3군, 4군 및 1군의 순으로 나타났고(p<0.05), 치경부에서는 2군과 3군이 1군과 4군에 비하여 현저히 높았다(p<0.05). The purpose of this study was to evaluate the micro-tensile bond strength (μTBS) of four luting resin to regional dentin of human primary teeth. Dentin from non-carious primary molars were prepared from different regions(s, superficial dentin; d, deep dentin; c, cervical dentin), and divided into groups based on anatomical locations and types of luting resins(Scotchbond Multi-purpose: SB ; One-Step: as ; AdheSE Bond: ASE ; G-Bond : GB) : SB-s, SB-d, SB-c; OS-s, OS-d, OS-c; ASE-s, ASE-d, ASE-c ; GB-s, GB-d, GB-c. Luting resins were used according to the manufacturers' instructions, to bond Light-Core™ Core Build-Up Composite) to the exposed dentin specimens in the light-curing mode. After storage for 1 day, μTBS was tested at a cross-head speed of 1 mm/min. Data were analyzed with T-test and two-way ANOVA. The bonding interface and fractography analyses were performed with SEM. The results were as follows : 1. μTBS to superficial dentin was significantly higher than to deep dentin for SB(p<0.05). But there were no significant differences in regional μTBS among as, ASK GB(p>0.05). 2. There were no significant differences in μTBS to superficial dentin among each groups. But, in deep dentin, μTBS of SB-d was significantly lower than those of OS-d, ASE-d, and GB-d(p<0.05). μTBS of OS-d was significantly higher than those of GB-d(p<0.05), but there were no significant differences in μTBS of ASE-d. There were no significant differences among μTBS of ASE-d, OS-d, and GB-d.

석면폐증 1례

이영수,장태원,유호대,정만홍,이용환,서지영,허 방,이재성 大韓産業醫學會 1999 대한직업환경의학회지 Vol.11 No.1

Asbestosis is the disease of pulmonary fibrosis caused by the inhaled asbestos fibers, and could be diagnosed clinically, in the case of exposure history to asbestos is proved, by clinical symptoms of dyspnea or dry cough, physical examonation findings, and the radiographic features. But many othder inorganic dusts would show similar findings in the chest radiogram and sometimes the exposure history is obscure, so for the exact diagnosis of asbestosis lung biopsy is needed. In Korea, there have been some reports of survey in the workplace where asbestos is handled or of asbestos related diseases. This is a case report of asbestosis with accompanying pleural plaques, who had the occupational exposure to asbestos for 30 years and the consistent clinical, radiographic and pathological findings in the lung tissue obtained by the videoscope assisted thoracoscopic biopsy(VATS).

하악전돌증환자의 악교정수술후 하악각변화에 관한 임상적 분석

권영호,장현중,이상한 대한악안면성형재건외과학회 2000 Maxillofacial Plastic Reconstructive Surgery Vol.22 No.2

Predictional study for lateral change between pre- and post-orthognathic surgery has been emphasized mainly on anterior area of lateral profile ; upper lip, lower lip and chin et al. So interest for lateral profile change has been less in posterior area of lateral profile and literature analyzing gonial angle change is rare. The purpose of this study is to make prediction for gonial angle change possible and to offer somewhat treatment guidance for gonial angle to be improved by investigating overall gonial angle change between pre- and post-orthognathic surgery and inquiring into factors influencing on pattern of genial angle change. For this study 35 patients were selected retrospectively. Lateral cephalometric radiographs were taken in just pre-op time, pod 1 day, pod 1 year. They were analyzed and genial angles were measured. The results were as follows : 1.Gonial angle at pod 1 day was decreased about 9.3˚than pre-op and gonial angle at pod 1 year was increased about 4.0˚than pod 1 day. So genial angle at pod 1 year was decreased about 5.3˚than pre-op genial angle(p<0.01). 2.Mean pre-op gonial angle was 129.4˚, showing significantly high value than normal and mean gonial angle at pod 1 year was 124.1˚, showing value near to normal. 3.Mean gonial angle change between pre-op and pod 1 year was decreased about 5.4˚ in female and 5.3˚ in male. There was no statistically significant difference between male and female(p>0.05). 4.Principal factor influencing on decreased gonial angle in gonial angle change between pre-op and pod 1 year was amount of mandibular setback. 5.Principal factor influencing on increased gonial angle in gonial angle change between pod 1 day and pod 1 year was % horizontal relapse, and it was thought that resorption and bone remodelling on posterior area in mandibular distal segment also were related to increased gonial angle. 6.It is thought that sagittal split ramus osteotomy in mandibular prognathic patients with high value of gonial angle is effective to improvement of gonial angle, and In patients who have normal range of gonial angle and are required with excessive mandibular setback, short lingual cut method, additional resection of posterior margin of distal segment, Obwegeser II method will be considerd. 7.More prudent operation and careful post-op management will be responsible for maintenance of post-operactive stable gonial angle.

황 첨착 활성탄에 의한 구리제거

김영환,박정훈,김정섭,유현철,이봉섭,곽명화,우성훈,박승조 東亞大學校 環境問題硏究所 2005 硏究報告 Vol.27 No.1

This study was carried out to remove copper ion in case of activated carbon impregnated sulphur(ACS) dose and different pH using activated carbon impregnated sulphur that prepared from activated carbon at sulphur dioxide atmosphere. Removal copper ion concentration was increased as increasing the ACS dose when copper ion concentration was 10 mg/L. Optimum dose of ACS 13.3 g ACS/L and maximum removal efficiency was 93%. The results obtained from adsorption experiment in the condition of ACS optimum dose when the pH was 3, 4, 5 respectively was pH 5 was excellent.

치과용 자성재의 안정성 개선에 관한 연구 : I. 치과용 자성재의 전기화학적 부식에 대한 스퍼터링 도금의 효과 I. Effects of Sputtered Film Deposition on the Electrochemical Corrosion of Dental Magnetic Materials

고영무,최한철,정재헌,정효수,최성욱 대한치과기재학회 1999 대한치과재료학회지 Vol.26 No.4

In order to investigate the various properties of dental magnetic materials studies on corrosion behavior, decreasing rate of magnetic flux density, amount of elements released, mean average surface roughness values and the changing of mean surface microhardness values for dental application were done. To increasing the corrosion resistance of dental magnetic materials, surfaces of Sm-Co and Nd-Fe-B based magnetic materials were plated with gold(Au), platinum(Pt), titanium(Ti), chromium(Cr) and cobalt(Co), and then electrochemical corrosion test were performed in 4 kinds of electrolyte solution(0.9% NaCl, 1% lactic acid, 0.05% HCl and modified Fusayama's artificial saliva). The results were as follows: 1. In Sm-Co and Nd-Fe-B based magnetic materials, the pitting corrosion of experimental group was higher than that of the control group, the experimental group was less corrosive than control hroup. 2. The amount of elements released from experimental group was more significantly decreased than that of control group. 3. Decreasing rate of magnetic flux density of experimental groups was significantly lower than that of control group after corrosion test. 4. The mean average surface roughness value of one(Sm-Co based magnetic materials not plated) of the control group was much increased in modified Fusayama's artificial saliva after first corrosion but that of the other(Nd-Fe-B based magnetic materials not plated) was much increased in 0.05% HCl solution. 5. The decreasing rate of surface microhardness values of experimental groups were lower than that of control group after corrosion, 6. Intergranular corrosion started first from the grain boundary of the control group and they advanced gradually to general corrosion, and the pitting corrosion occurred locally at area of existing pits in experimental group.

원발성 조기 위 소세포암

송근영,강길,박정희,김경종,민영돈,박치영,임성철 朝鮮大學校 附設 醫學硏究所 2002 The Medical Journal of Chosun University Vol.27 No.2

Gastric small cell carcinoma(SCC) is defined as poorly-differentiated neuroendocrine tumor of the stomach, and their morphologic and biologic features arc similar to those of the lung. It is known that this tumor has poor prognosis even in early pathologic stage, It acounts for only 3% of all gastrointestinal SCC. that has been reported only 33 cases outside and 6 cases inside of the comitiy. Most of these reports are related to advanced gastric SCC. as pathologic stage IIIb or IV. This report describes a case of early gastric SCC. pathologic stage Ib.