Curcumin ameliorates cadmium-induced nephrotoxicity in Sprague-Dawley rats

Kim, Kyeong Seok,Lim, Hyun-Jung,Lim, Jong Seung,Son, Ji Yeon,Lee, Jaewon,Lee, Byung Mu,Chang, Seung-Cheol,Kim, Hyung Sik Elsevier 2018 Food and chemical toxicology Vol.114 No.-

<P><B>Abstract</B></P> <P>Chronic exposure to cadmium (Cd) causes remarkable damage to the kidneys, a target organ of accumulated Cd after oral administration. The aim of the present study was to investigate the protective effect of curcumin against Cd-induced nephrotoxicity. Sprague–Dawley male rats were divided into the following four treatment groups: control, curcumin (50 mg/kg, oral), CdCl<SUB>2</SUB>, (25 mg/kg, oral), and pre-treatment with curcumin (50 mg/kg) 1 h prior to the administration of CdCl<SUB>2</SUB> (25 mg/kg, oral) for 7 days. At 24 h after the final treatment, the animals were killed, and the biomarkers associated with nephrotoxicity were measured. Our data indicated that blood urea nitrogen (BUN) and serum creatinine (sCr) levels were significantly reduced by curcumin pre-treatment in CdCl<SUB>2</SUB>-treated animals. Histopathological studies showed hydropic swelling and hypertrophy of the proximal tubular cells in the renal cortex after Cd treatment. Pretreatment with curcumin ameliorated the histological alterations induced by Cd. The urinary excretion of kidney injury molecule-1 (Kim-1), osteopontin (OPN), tissue inhibitor of metalloproteinases 1 (TIMP-1), neutrophil gelatinase-associated lipocalin (NGAL), and netrin-1 significantly reduced by curcumin treatment compared to that in the CdCl<SUB>2</SUB>-treated group. The administration of curcumin provided a significant protective effect against Cd-induced nephrotoxicity.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Curcumin protects against cadmium-induced renal injury. </LI> <LI> Curcumin reduces urinary excretion of AKI biomarkers. </LI> <LI> Curcumin protects against cadmium-induced apoptosis in the kidney. </LI> </UL> </P>

한국인 알코올 의존 환자에서 알코올 대사 효소 유전자형 빈도의 남녀 차이

김성곤,김철민,이덕기,황인복,이현숙,김성연,전은숙,송영상,박제민,최병무,김명정 大韓神經精神醫學會 2005 신경정신의학 Vol.44 No.2

Objectives : There are a number of preceding epidemiological studies reporting gender differences in the genetic etiology of alcohol dependence. The author investigated gender difference in the frequencies of ADH2 and ALDH2 genoypes between the patients with alcohol dependence and normal control. Methods : The subjects were 141 alcohol dependent patients (104 males, 37 females) and 138 normal control (79 males, 59 females). The frequencies of 1/1 and 1/2+2/2 (2+ afterward) genotypes for ADH2 and ALDH2 were investigated in male and female between alcohol dependence and normal control group. DNA was extracted from WBC in peripheral venous blood and PCR-RFLP method was used out for genotyping. Results : First, the frequency of ADH2 1/1 genotype was significantly higher in alcohol dependent patients than normal control in both genders. Second, while there was no gender difference in the frequency of ADH2 1/1 genotype in normal controls, in the patient group however, the frequency was significantly higher in females than males. Third, in male subjects with alcohol dependence, the frequency of ALDH2 1/1 genotype was significantly higher than in male normal control subjects. On the other hand, in female subjects with alcohol dependence, the frequency of ALDH2 2+ genotype was significantly higher than in female normal control subjects. Conclusion : These results suggest that while the risk of alcohol dependence is predominantly affected by ALDH2 1/1 geno-type in male, the female ADH2 1/1 genotype is mainly associated with the risk of alcohol dependence. This means that there are gender differences in the genetic etiology of alcohol dependence.

인천지역 TDI 노출 작업자에서 직업성천식 유병율

김형렬,김철우,홍윤철,전형준,김치년,김현수,이지나,신주연,고동희,노재훈 大韓産業醫學會 2004 대한직업환경의학회지 Vol.16 No.2

목적 : TDI는 직업성천식을 일으키는 대표적인 물질로 알려져 있다. 과거 TDI에 노출된 작업자들의 천식 발생률을 5~6%가량 보고하였고, 국내에서도 2-20%에 이르는 유병율을 보고한바 있다. 작업환경이 많이 개선되고, 노출량이 감소된 최근에도 TDI에 의한 직업성천식에 대한 보고가 많이 이루어지고 있어, 이에 대한 적극적인 조사와 진단과정을 통해 유병율을 구하고 질병발생의 변화양상을 파악해 보고자 하였다. 방법 : 인천지역에서 특수건강진단을 수행하는 2개 기관의 TDI 노출 근로자 400여명 중, 사업장의 규모가 50인 이상이며 사업주가 연구진행을 허락한 사업장에서 근무하는 근로자 중 직접적으로 TDI에 노출되는 작업에 종사하는 근로자 170명을 연구대상으로 하였다. 이들에 대해 설문지와 산업의학의사의 면담을 통한 작업관련성 평가를 근거로 정밀대상자를 선정하고 이들에 대해 메타콜린 유발시험, TDI 유발시험 등을 수행하여 직업성천식을 확인하였다. 결과 : 설문조사 결과 11명이 직업과 관련하여 천식증상을 보이는 것으로 나타났고(6.9%), 이중 7명에서 메타콜린 유발검사 양성소견을 보였다. TDI에 의한 특이유발시험 결과 1명에서 양성반응이 나타나, 이 집단에서 TDI에 의한 직업성천식의 유병율은 0.58%였다. 결론 : 과거 국내연구에서 TDI에 의한 직업성천식의 유병율이 2~20%에 이르렀던 데 비해, 본 연구에서는 0.58%로 나타났다. 이는 국외 논문에서도 나타나는 경향으로 작업장 노출량 감소로 점차 발생률이 감소하는 양상을 보이고 있다. 그러나 이미 증상이 나타난 근로자들이 부서를 옮기거나, 직장을 그만둠으로 인해 그 유병율이 과소평가되었을 가능성도 있다고 판단된다. Objecdives: This study was carried out to estimate the prevalence of isocyanate-induced occupational asthma in toluene diisocyanate (TDI) exposed workers. Methods: We examined 170 workers who had been directly exposed to TDI through a medical questionnaire, physical examination, and pulmonary function test. Based on screening examination, workers with suspected occupational asthma were selected for further evaluation such as methacholine and TDI challenge tests. Results: Eleven (6.9%) among 170 workers complained of symptoms of occupational asthma. and 7 among these 11 symptomatic workers showed positive responses to the methacholine challenge test (4.1 %). One spray painter was confirmed as having the TDI induced occupational asthma following a positive response to TDI challenge test. Conclusions: The prevalence of TDI-induced asthma was at 0.58% was lower than that for former studies (2-20%). Improved workplace environment, lower level of TDI exposure compared to the past, and the healthy workers effect may have contributed to this low rate of asthma prevalence in workers with TDI exposure.

2003년 국내 중증급성호흡기증후군 진료 현황 및 문제점 분석

이진수,김은실,정문현,백제중,정선화,안주희,최영화,이선희,고철우,김성범,김민자,박승철,기현균,송재훈,최상호,김양수,이상오,조용균,박영훈,정숙인,김연숙,이흥범,손창희,장성희,정희진,김우주 대한감염학회 2004 감염과 화학요법 Vol.36 No.3

목적 : 2002년 말 중국에서 SARS가 발생한 이후 국내에서도 2003년 10월까지 총 3명의 추정환자, 17명의 의심환자가 보고되었다. 향후 추가적인 SARS의 유행이 우려되는 상황에서, 그간의 SARS 환자 진료에 있어서의 실질적인 준비사항, 진료 현황 등에 대한 조사를 통해 문제점을 파악하여, 향후 더 나은 대비가 될 수 있도록 개선점을 제시하고자 하였다. 재료 및 방법 : SARS로 의심되는 환자를 진료 경험이 있는 병원의료진을 대상으로 2003년 10월에 설문조사를 실시하였다. 설문에는 SARS 환자 진료 시의 실질적인 조치, 진료 현황, 병실, 응급실 및 외래에서의 격리 시설과 준비사항, 보건당국의 관리와 지원에 관한 사항을 포함하였다. 결과 : 대상이 되는 22개 병원 중 17개(17/22, 77.2%) 병원이 설문에 응하였다. SARS 환자를 위한 격리실은 응급실, 외래, 일반병실 및 중환자실에서 각각 9개(9/17, 52.9%), 5개(5/17, 29.4%), 15개(15/16, 93.7%), 4개(4/16, 25%) 병원에서 음압처리가 되어있지 않은 일인실 혹은 다인실이 사용되었고, 1개(1/16, 6.3%) 병원에서만 일반병실에서 음압격리실이 운영되었다. 입원환자의 진찰 시 개인보호구의 착용은 거의 모든 의료기관에서 이루어졌다. 보건당국에서 SARS지정병원의 시설 등을 사전에 확인한 곳은 1곳(1/12, 8.3%)이였고, 14개 병원(14/15, 93.3%)에서는 보건당국에 의뢰한 검사결과를 통하 받지 못하였다. 결론 : 의료기관에서 SARS 환자용 격리실뿐만 아니라 기존의 격리실 설비 등이 미흡하였으며, 특히 중환자실 및 외래의 준비가 더욱 부족하였다. 보건당국의 의료기관에 대한 종합적인 지원이 부족하였고, 병원과의 원활한 연계가 잘 이루어지지 않았다. SARS 만이 아닌 격리를 필요로 하는 질환의 적절한 진료를 위해 향후 병원 시설의 정비와 정부차원에서의 보다 구체적이고 실질적인 대책마련이 필요하다. Background : There was an worldwide outbreak of the Severe Acute Respiratory Syndrome (SARS) originated from China in late 2002. During that period three cases of suspected SARS and 17 cases of probable SARS were reported in Korea. With the concerns about the reemergence of SARS-coV transmission, it is important to be prepared for any possibility. So, this study is aimed to analysis the past measures in managing SARS and propose the amendatory plans to improve the preparedness. Materials & Methods : Questionnaires were collected among clinicians with any experience in managing the probable or suspected SARS cases in Oct. 2003. 17 out of 22 hospitals responded to the questionnaire. The contents in the questionnaire were practical activities, personal equipments, response plans, isolation facilities in emergency centers, outpatient clinics, general wards and intensive care units, and relationship with the public health department. Results : The dedicated isolation rooms in emergency centers, outpatient clinics, general wards, and intensive care units were prepared in 9 (9/17, 52.9%), 5 (5/17, 29.4%), 15 (15/16, 93.7%), and 4 (4/16, 25.0%) hospitals, respectively. Except for one hospital that newly made negative pressure room for SARS, single or multi-bed rooms without airborne infection control were used in all the other hospitals. The personal precaution principles were kept quite well in general wards. Before the designation of SARS hospital by the public health department prior evalution to see if the hospital was suitable for managing SARS was conducted in only 1 (1/12, 8.3%) hospital. The results of laboratory diagnosis were reported back in 1 (1/15, 6.6%) hospital. Conclusions : The isolation facilities which can control airborne infection were almost deficient not only for SARS but also for other respiratory transmissible diseases. For the infection control of transmissible diseases including SARS, more investment is needed on medical facilities and comprehensive support from the public health department required.

셀 패킹이 MPEG 비디오의 화질에 미치는 영향

김장형,최연성,송왕철 濟州大學校 産業技術硏究所 1996 산업기술연구소논문집 Vol.7 No.1

In ATM-based B-ISDN, the traffic characteristics for the video signal are quite different from those of the data andor voice. Serious problems of the quality of picture is brought due to a little cell loss. The output bits stream of MPEG encoder consists of macroblocks of different lengths. In this paper, we analyze packing methods of these macroblocks into ATM cell. Two kinds of packing approach, which are loosely and closely packing, to pack the video signal to ATM cell are considered. Loosely packing increases traffic in the networks. But it is shown that while due to cell loss for high speed video service loosely packed cell is robust in quality than closely one. We prove these theoretical approach and simulation results for real MPEG video.

응급실에서 시행된 기관내 삽관의 현황

김병철,강보승,송형곤,이정훈,송근정,정연권 대한응급의학회 1999 대한응급의학회지 Vol.10 No.4

Background : The purpose of this study was to analyze the endotracheal intubation cases performed in the emergency department. Methods : We investigated retrospectively 326 cases of endotracheal intubation performed in the emergency department of a tertiary care center form April 1, 1998 to March 31, 1999. We focused on operators, medications used, its success rate and immediate complications, and the relationship between its success rate and medications. Results : Of 326 consecutive intubations, 193 patients(59.2%) were done by emergency medicine residents or attending physician. While 320 patients(98.2%) were successfully intubated, 6 patients could not be intubated and 2 patients underwent tracheostomy. Of 50 cases of intubations(15.3%) attempted with paralyzing agents, 48 cases were done with succinylcholine and 46 cases underwent by emergency physicians. Intubations with neuromuscular paralysis resulted in high success rates at the first attempt. Of 55 immediate adverse events were encountered in 47 patients(desaturation=17, bronchial intubation=15, hypotension=8, bradycardia=4, cardiac arrest=2, others=5). Conclusion : At this institution, paralyzing agents were used infrequently, but almost all of them were used by emergency physicians.

철원산 현무암을 이용한 폴리머 콘크리트의 물리·역학적 특성

연규석,이윤수,김철영 강원대학교 부설 석재복합신소재 제품연구센터 1999 석재연 논문집 Vol.4 No.-

이 연구는 철원지역에 분포하는 현무암을 대상으로 부가가치를 향상시킬 수 있는 상품을 개발하기 위한 기초자료를 얻기 위하여 현무암에 대한 암석학적 및 물리적. 화학적 특성을 규명하고자 하였다. 철원지역의 현무암은 크게 대다공상 조직과 소다공상 조직을 갖는 2가지 암상으로 구분할 수 있으며, 주로 사장석과 휘석류, 감람석, 불투명 광물 등으로 구성되어 있고, 일부 기공속에서는 석영과 방해석이 발견된다. 현무암의 비중(참비중)은 2.89∼2.94, 공극률과 흡수율은 각각 9.96∼13.7%, 4.01∼6.53% 값의 범위를 보였고, 마모감량은 24.7∼30.5wt.%, 일축 압축강도와 인장강도는 각각 536∼875㎏f/㎠, 53.7∼69.1㎏f/㎠의 값이므로, 강도의 경우 기공이 적은 현무암이 많은 현무암보다 약간 더 강한 것으로 나타났다. 현무암의 미량원소 함량중 Co, Cr, Ni 등의 함량은 KIoke(1979)가 제시한 농작물에 독성을 줄 수 있는 토양중의 최대 허용한계값 (Co 50 ppm, Cr 100 ppm, Ni 100 ppm) 보다 더 높은 값을 보여, 철원현무암을 석제품으로 가공할 때 생기는 미석분을 처리할 때에는 이러한 점을 충분히 고려하는 것이 바람직할 것이며, 현무암의 분말시료에 대한 양이온 교환 용량 값에 의하면 대다공상 현무암 (5.81meq/100g)의 경우 중금속 흡착제로 활용할 수 있는 가능성이 충분히 있어, 이에 대한 차후의 면밀한 연구가 필요할 것으로 판단된다. Feasibility of chulwon basalt as a coarse aggregate for polymer concrete was investigate to solve the environmental problems associated with the waste. Basalt aggregates were prepared and used to produce basalt polymer concrete. The concrete was tested for physical and mechanical properties. The tested properties of basalt polymer concrete were not different from those of normal polymer concrete. It was concluded that chulwon basalt could be used as an aggregate for polymer concrete production. However, the long-term behavior, thermal extension and durability of basalt polymer concrete were suggested to be further explored.

폴리머 모르터의 난연성 개선에 관한 연구

연규석,大濱嘉彦,김남길,김철영,지경용 강원대학교 부설 석재복합신소재 제품연구센터 2000 석재연 논문집 Vol.5 No.-

불포화 에스트테르 수지의 큰 이용분야의 하나로 건축재료가 있으나 여기서 가장 문제로 되어 있는 것이 바로 난연성의 향상이다 불포화 폴리에스터수지는 탄소를 구성성분으로 하는 유기물이므로 무기재료와 같은 불연성을 기대하기는 어려우나 어느 정도까지는 연소를 억제시킬 수는 있다. 본 연구에서는 UP를 결합재로 하고 탄산칼슘을 충진재로, 규사를 잔골재로 하는 폴리머 모르터를 제조하고 이에 첨가되는 난연제량과 UP량을 각각, 5~12.5%, 12~24%로 변화시켜 난수별 난연특성을 고찰하였던 바, UP사용량에 따른 특정 난연제의 최적 사용량 범위를 얻을 수 있었다. One of the fields using the unsaturated polyester resin is the constructional material, but the most serious problem, in this field, is the incombustibility improvement. it is hard to expect the noncombustibility as in the inorganic materials for the unsaturated polyester resin since whose structural material is carbon, but the combustion can be controlled up to some degree. In this study the polymer mortars were made in which the unsaturated polyester resin as binder, the calcium carbonate as filler and the silica sand as fine aggregate. The incombustible characteristics were then investigated as varying the amount of the flame retarder and the unsaturated polyester resin from 5 to 12.5% and 12 to 24%, respectively. Based on the results, the optimized range of amount of flame retarder used with various amount of unsaturated polyester resin could be obtained.

SD Rats를 이용 1-hexene의 흡입독성 연구

김현영,임철홍,정용현,이권섭,이성배,이준연,한정희,전윤석,이용묵 한국환경독성학회 2001 환경독성보건학회지 Vol.16 No.4

The purpose of this study was to investigate the acute (4 hours) and repeated-dose (6 hours a day, 5 days a week, 4 weeks) toxic effects of I -hexene on Sprague- Dawley (SD) rats which were treated by inhalation. The results were as follows; I. The median lethal concentration (LC_(50)) was estimated 52,694 ppm (confidence limit 95%; 49,494~55,447 ppm) in acute inhalation. Abnormal clinical signs related to the l-Hexene were not observed with the acute inhalation dose. Gross findings of necropsy revealed on evidence of specific toxicity related to the 1-hexene, II. By repeated inhalation exposure the body weight of male were more or less reduced by the dose of 2,500 ppm and 5,000 ppm compared with control group. However there were no significant variation hematology and blood biochemistry for the exposed rats compared with the control rats. Abnormal clinical signs and gross findings of necropsy related to the 1-hexene were not shown. In conclusion when we exposed I-hexene to SD rats for 4 weeks, 5 days per week, 6 hours per day, the Lowest observed effect level (LOEL) was over 2,500 ppm and Non observed effect level (NOEL) was below 500 ppm.

광릉수목원 혼합림에서 복사 에너지의 계절 변화 특성

김연희,조경숙,김현탁,엄향희,최병철 한국대기환경학회 2003 한국대기환경학회지 Vol.19 No.3

The measurement of the radiation energy, trunk temperature, leaf area index(LAI), air temperature,vapor pressure, and precipitation has been conducted under a mixed forest at Kwangneung Arboretum during the period of 2001. Characteristics of the diurnal and seasonal variation of the radiative energy were investigated. The aerodynamic roughness length was determined as about 1.6m and the mean albedo was about 0.1. The downward shortwave radiation was linearly correlated with the net radiation and its correlation coefficient was about 0.96. From this linear relation, the heating coefficient was calculated and its annual mean value was about 0.21. The albedo and heating coefficient was varied with season, surface characteristics, and meteorogical conditions. The diurnal and seasonal variations of radiation energy were discussed in terms of the surface characteristics and meteorological conditions. In the daytime, during clear skies, net raditaion was cominated by the shortwave radiation. In presence of clouds and fog, the radiation energy was diminished. At night, the net radiation was entirely dominated due to the net longwave radiation. There was no distinct diurnal variation in net radiation flux during the overcast or rainy days. The net radiation was strongest in spring and weakest in winter. The seasonal development in leaf area was also reflected in a strong seasonal pattern of the radiation energy balance. The timing, duration, and maximum leaf area and trunk temperature were found to be an important control on radiation energy budget. The trunk temperature was either equal or warmer than air temperature during most of the growing season because the canopy could absorb a substantial amount of sunlight. After autumn(after the middle of October), the trunk temperature was consistently cooler than air temperature.