신경세포성장인자로서 척수운동신경세포의 손상에 미치는 한약재의 약류별 효능 및 기전에 관한 연구

鄭遇悅,朴承澤,成彊慶,李星根 대한동의병리학회 1998 동의생리병리학회지 Vol.12 No.2

산소자유기가 흰쥐의 배양 운동신경세포에 미치는 세포독성에 대한 기전을 규명하기 위하여 여러 농도의 산소자유기를 배양 신경세포에 처리한 후 산소자유기의 신경독성 효과를 분석하였으며 또한 산소자유기에 의하여 유발된 신경독성에 대한 한약재중 보혈약류와 보기약류의 대표적인 처방인 사군자탕과 사물탕의 신경세포독성의 방어효과를 MTT assay법에 의하여 조사하여 다음과 같은 결론을 얻었다. Xanthine oxidase(XO)와 hypoxanthine(HX)은 처리한 농도에 비례하여 배양 신경세포의 생존율을 현저하게 감소시켰으며, 또한 사물탕과 사군자탕이 XO/HX의 독성효과를 유의있게 방어하였다. 이상의 결과로부터 산소자유기는 배양 소의 희소돌기아교세포에 독성을 나타냈으며 사군자탕과 사물탕등의 한약추출물이 산소자유기의 독성을 방어하는데 효과적인 것으로 나타났다. In order to elucidate the neuroprotective effect of Sagunjatang and Samultang on oxidant-induced neurotoxicity in cultured rat spinal motor neurons, the colorimetric assay such as MTT assay was performed and light microscopic study was also carried out after cultured spinal motor neurons from neonatal rat were treated with the medium containing various concentrations of xanthine oxidase(XO) and hypoxanthine(HX) for 3 hours. In addition, protective effect of herb extracts on the neurotoxicity induced by oxygen radicals was examined in these cultures. The results were as follows : Cell viability of cultured rat spinal motor neurons which were exposed to various concentrations of 30mU/ml XO and 0.1mM HX for 3 hours, was decreased in a dose-dependent manner. MTT50 value was 30mU/ml XO and 0.1mM HX after motor neurons were incubated with various concentrations of XO/HX for 3 hours. Cultured spinal motor neurons showed degenerative changes such as the decrease of cell number and loss of cell processes when neurons were cultured in the medium containing 30mU/ml X0 and 0.1mM HX for 3 hours. Oxygen radicals were toxic on cultured rat spinal motor neurons by the decrease of cell viability. Herb extracts, Sagunjatang and Samultang prevented the oxidant-induced neurotoxicity significantly after spinal motor neurons were preincubated with herb extractions for 2 hours before exposure of oxygen radicals. These results suggest that oxygen radicals have neurotoxic effect on cultured spinal motor neurons derived from neonatal rat, and selective herb extracts such as Sagunjatang and Samultang are very effective in protecting the neurotoxicity induced by oxygen radicals.

의료용 조합자극기(HAPPY DREAM HM-206DB) 적용이 동결견 환자의 통증과 관절가동범위에 미치는 영향

홍성찬,김영빈,박병근,김유성,이성진,정동혁 원광대학교 체력과학연구소 2008 體力科學硏究 Vol.30 No.1

The purpose of this study is to find out the effectiveness of the HAPPY DREAM HM-206DB applied to the patients with Frozen Shoulder by measuring, assessing and analyzing the changes in pain and range of motion(ROM). Surveyed from Mar. 2007 to Dec. 2007 were 10 patients suffering from Frozen Shoulder. After HAPPY DREAM HM-206DB, the pain were measured and analyzed by means of MPQWL, VAS-I and VAS-U, while ROM was measured and analyzed by using Goniometer. The major findings from this study are as follows; 1. There was significant decrease in the MPQWL after HAPPY DREAM HM-206DB applied(p<.001). 2. There was significant decrease in the VAS-I(intensity of pain) after HAPPY DREAM HM-206DB applied(p<.001). 3. There was significant decrease in the VAS-U(unpleasantness of pain) after HAPPY DREAM HM-206DB applied(p<.001). 4. There appeared, however, significant increase in ROM(abduction, external and internal rotation) after HAPPY DREAM HM-206DB applied(p<.001). From the above outcomes, it was revealed that HAPPY DREAM HM-206DB application has proven to reduce the pains, exercising huge influence to increase ROM. It is, therefore, suggested to continue and expand the study on the cure of Frozen Shoulder and to motivate patients, rehabilitation specialists, and health management specialists etc. to consider HAPPY DREAM HM-206DB application as safest and most efficient pain remedy.

스페로이드 조합자극치료 적용 후 만성요통의 통증 감소 효과

홍성찬,김영빈,박병근,김유성,이성진,정동혁 원광대학교 체력과학연구소 2008 體力科學硏究 Vol.30 No.1

The aim of the present study is to evaluate effects of Spheroid 9000NS applied to 10 elderly patients with Chronic Low Back Pain. The effects were measured and analyzed by means of MPQWL, VRS, VAS-I and VAS-U after 4, 8 and 12 weeks, respectively, during the 12-week application of the Spheroid 9000NS. From the findings of the measurement and analysis, following conclusion could be drawn: 1. Significant reductions in MPQWL were confirmed at each period of measurement since the use of Spheroid 9000NS(p<.001). 2. Significant reductions were also revealed in VRS at each period of the measurement(p<.001). 3. Significant reductions were also revealed in VAS-I at each period of the measurement(p<.001). 4. The same reductions were found as well in VAS-U 4, 8 and 12 weeks after the application of the Spheroid 9000NS(p<.001). In sum, it may be concluded from the outcomes that the use of Spheroid 9000NS may help reduce drastically Chronic Low Back Pain, mostly effective to remove them speedily and safely. Patients, rehabilitation therapists and health experts are, therefore, recommended to employ the products as safe and effective means to control pains. Continued researches and experiments should further be conducted to collect more data from the larger population of patients with particular diseases in clinical situation, though.

알코올의 진정 효과와 일부 인지 기능에 미치는 영향

호정일,왕성근 대한생물치료정신의학회 2003 생물치료정신의학 Vol.9 No.1

Objectives : The purpose of this study was to evalute the effect of alcohol on sedation and some cognitive function(eg,daytime sleepiness, fatigue, and mood measured by multiple sleep latency test(MSLT) and visual analogue scale(VAS), vigilance, attention and short-term memory measured by Vienna Test System). Methods : 15 healthy, normal young college students participated in this study. Participating subjects were in good physical and psychological health and were asymptomatic as to sleep/wake complaints. Subjects visited our laboratory by 08 : 00 at the first day, and they had baseline MSLT and Test(at 9;00, 11;00, 14:00, and 16:00).Each time, the subjects completed VAS for sleepiness, fatigue and mood before they underwent an Vienna Test to evaluate their vigilance, attention and short-term memory. On the second day, subjects had 0.5g/kg of alcohol, the third day, subject had 1.0g/kg of alcohol, and repeated the same process as those of the first. Results : 0.5g/kg of alcohol reduced sleep latency after 1 hour, and 1.0g/kg of alcohol reduced sleep latency until 5 hours after administration significantly. 0.5g/kg of alcohol induced daytime sleepiness 1 hours after administration, and 1.0g/kg of alcohol induced daytime sleepiness and fatigue until 3 hours after administration, but there were no effects on mood. Vigilance was decreased significantly 1 hour after 0.5g/kg of alcohol administration, and attention and short-term memory were not changed significantly after alcohol administration. After alcohol adminstration(0.5-1.0g/kg), there were significant positive correlation between daytime sleepiness and fatigue, and fatigue and mood. Conclusion : It can be concluded that alcohol decreases sleep latency and induces daytime sleepiness and fatigue, and decreases vigilance in normal subjects.

운동기간에 따른 혈중지단백대사 및 비만호르몬의 변화

홍성찬,함용기,김영빈,박병근,정동혁,이정윤,권성택 圓光大學校 附設 體力科學硏究所 2003 體力科學硏究 Vol.26 No.2

The purpose of this study is to elucidate the effects of regular swimming exercise on blood lipoprotein metabolism and leptin. In this study, swimming(HRmax 50-75%, 60min/day, 5day/week) was performed and 18 were participated for study during 16 weeks. They were divided into two groups, one group as experimental group(n=9) and the other as control group(n=9). A measured factors were TC, TG, HDL-C, LDL-C and leptin.. All data were expressed as mean± standard deviation by using spss package program(win 9.0), one way anova and independent t-test was used to analyze the difference of exercise duration(0week, 8week, 12week, 16week) between groups. For all statistics performed statistical significance was set at p<.05. After exercise intervention, TC, TG, HDL-C, LDL-C and leptin were found to have more significant effects in the experimental group than control group. This results indicated regular swimming exercise training is beneficial to prevent and treat cardiovascular disease and obesity.

Streptomyces sp. Y9343이 生産하는 齒面細菌膜 分解酵素의 精製와 特性

김성주,한홍근,윤정원 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.1

치면세균막을 제거하거나 형성을 억제하여 치아우식증 예방제를 개발할 목적으로 α-1,3 glucanase를 분비하는 새로운 균주로 부터 분리 정제하여 그 특성을 조사하였다. 불용성 glucan을 유일탄소원으로 하는 agar plate를 제조하여 토양으로 부터 α-1,3 glucanase 분비균주를 탐색한 결과 Streptomyces sp. Y9343을 얻었다. 액체배양시 최적 효소생산조건은 탄소원으로 1% soluble starch와 indcer로 0.5% insoluble glucan을 첨가하였을 때 가장 효율적이었다. α-1,3 glucanase는 황산 암모늄 염석, DEAE-CEllulose 이온교환 크로마토그래피, Sephadex G-75 겔 여과 등에 의하여 32.1배까지 정제되었고 수율은 0.53%이었으며, 이 때의 활성도는 7840.9 U/mg protein이었다. 정제된 α-1,3 glucanase를 SDS-PAGE로 분석한 결과, 단일체임을 확인하였으며, 이 때 분자량은 22,500이었다. 효소의 최적 pH는 6.5이었다. 효소의 최저온도는 37℃이었고, 열에 대한 안정성은 70℃ 이상에서 40%~60%의 효소활성이 상실함을 보였다. Detergent의 영향은 SDS에 의해 83%, Tween 20에 의해서는 약 27% 정도의 활성저해를 받았다. 효소활성의 금속이온에 의한 영향은 Co^2+, Mn^2_+에 의해 각각 81.8, 69.7%의 활성의 증가를 보였고 이들의 최적농도는 10mM이었으며, 반면에 Hg^2+에 의해서는 93.9%의 효소활성의 저해를 나타내었다. 또한 초기속도(30분 이내)에 금속이온에 의한 영향이 큰 것으로 나타났다. α-1,3 glucanase의 불용성 glucan에 대한 K_m 값은 2.50mM이었고, V_max는 0.0431 mM/min이었다. α-1,3 glucanase의 기질특이성을 조사한 결과, 반응 30분 후 IG와 soluble starch에는 각각73, 100%의 높은 분해력을 보였으며, raw starch, dextran T-10에 대해서는 낮은 분해력을 보였다. 한편, 인조치면세균막을 S. mutans로부터 시험관 벽에 제조한 후, α-1,3 glucanase를 처리한 결과 2시간 이내에 완전히 분해 제거되는 것을 알 수 있어 강력한 치아우식예방제로 개발될 수 있음을 보여 주었다. Streptococcus mutans has been implicated as primary causative agents of dental caries by insoluble glucan (IG) in human and experimental animals. An attempt was made to search for the α-1,3 glucanase that degrades IG produced by S. mutans. α-1,3 glucanase was detected in the culture supernatant of microorganisms, which are isolated from soils on agar medium containing IG as a sole carbon source. This Streptomyces sp. hydrolysed IG produced by immobilized S. mutans and was named as Y9343. This enzyme required α-1,3 glucan (IG) as an inducer. The optimum conditions for enzyme production were studied. The enzyme was purified by 30~70% (NH_4)_2SO_4 precipitation, anion exchange chromatography on DEAE-cellulose and gel filration on Sepadex G-75. The purified enzyme has a specific activity of 7840.0 U/mg protein giving 32.1-fold purification and final yield of 0.53%. The molecular weight was estimated to be about 22.5 kDa by SDS-PAGE. The optimum pH and temperature for enzyme reactiorr were 6.5 and 37℃, respectively and the enzyme was relatively stable at the temperature below 60℃. The activity of purified enzyme was enhanced by adding Co^2+, Mn^2+, and Mg^2+ into the medium, whereas inhiited by adding Hg^2+, Zn^2+ and SDS. The K_m and V_max value of α-1,3 glucanase for IG were estimated to be 2.50 mM and 0.0431 mM/min, respectively. The thin layer chromatographic analysis of hydrolysates from IG with α-1,3 glucanase showed that glucose was the main product of reaction. This enzyme activity was about 14 times higher than marketing dextranase as preventive agent against artificial dental caries by S. mutans in TH medium including 5% sucrose after 30 minutes.

정상 성인에서 수면박탈이 수면구조에 미치는 영향

왕성근,이정규,최하석 충남대학교 의과대학 지역사회의학연구소 1991 충남의대잡지 Vol.18 No.2

To identify the effect of total sleep deprivation on sleep structures, polysomnography was performed for 8 healthy young adults. The subjects were young adults, ranging in age from 21 years to 26 years, with no past or present histories of significant medical, neurological, or psychiatric illness as well as no current major sleep disturbances or parasomnias, After one adaptation night, each subject was recorded polysomnography in the sleep laboratory. Sleep records were analyzed according to the criteria of Rechtschaffen and Kales every 20 seconds, and tried paired-t-test. The results were summarized as follows. 1. After on night of total sleep deprivation, compared with the baseline WASO was significantly decreased in the first recovery night, and SWS and REM sleep were significantly increased in the first recovery night. 2. Compared with the baseline, the relative value(%) of REM sleep was significantly increased in the first recovery night. 3. Compared with the baseline, TST was significantly increased in the first recovery night, and sleep latency and REM latency were significantly decreased in the first recovery night. According to the above results we could identify the effects of total sleep deprivation on sleep structure in normal young adults.

국내에서 분리된 Extended-Spectrum β-Lactamase 생성 Escherichia coli와 Klebsiella pneumoniae의 빈도 및 유형

홍성근,김선주,정성훈,장철훈,조성란,안지영,신종희,이혜수,송원근,어영,염종화,용동은,이경원,정윤섭 대한임상미샐물학회 2003 Annals of clinical microbiology Vol.6 No.2

손상정도계수와 외상치를 이용한 외상환자의 분석

송근정,장문준,김성중,조광현,김인병,최성욱,이한식 대한응급의학회 1993 대한응급의학회지 Vol.4 No.2

Trauma is one of the most common causes of death. Recently, injury severity score(ISS) is widely used to evaluate and classify the patients with multiple injury. Trauma score(TS) was developed as a means of providing rapid accurate assessment of the injured patient. Authors conducted a review of trauma patients who visited the emergency department of YongDong Severance Hospital from September 1992 to August 1993. ISS and TS were collected from comprehensive initial evaluation. The results were as follows : 1) The subjects were 10.2%(2,666 patients) of total 26,202 emergency patients. 2) Male to female ratio was 2.1 : 1. The age distribution was highest under 10 year(24.9%). The mean ISS was 3.5, and the mean ISS increased with ago. 3. Analysis of injury mechanism showed high incidence of traffic accident(35.3%), blunt trauma 25.7%, fall 24.2%, stabbing 10.7%, and others 4.3% in order. 4. The 8.0% of patients were admitted after initial management and their mean Iss was 12.6 mean TS was 15.5. 5. Among admitted traffic accident patients, the mean ISS of passenger T.A., pedestrian T.A. and driver T.A. were 19.0 ±9.9, 14.4 ±10.6, and 11.3 ±7.7 respectively. 6. Among 42 mortality cases, 25 patients were D.O.A. and 17 were expired. The mean ISS of D.O.A. Patients was 30.0; the mean Iss and mean Iss and mean TS of expired patients were 28.3 and 11.8 respectively. 7. The most common cause of death was head trauma(66.7%). 8. By comparing the ISS and TS of admitted and expired patients, mortality occurred above 16 point of ISS. The mortality increased with increasing ISS, but it was influenced by the trauma site and the severity of the particular injury site. Mortality also occurred below 15 point of TS, the mortality increased inversely with TS, and the mortality with TS, and the mortality was high below 13 point (above 40%).

Spheroid 조합자극치료 적용이 신체구성에 미치는 영향

홍성찬,김영빈,정동혁,박병근,김유성,이성진 원광대학교 체력과학연구소 2008 體力科學硏究 Vol.30 No.1

The present study aims to explore the effects of applying spheroid-combined stimulating therapy on body composition. For this aim. 20 persons were selected as subjects and 10 of them each were assigned to the experimental group and control group, respectively. The experimental group was applied a 12-week combined stimulating therapy and changes in physical constitution of the members were measured before application and 4, 8 and 12 weeks after application. From the study, following conclusions could be drawn: First, body weights of the experimental group decreased from 59.9kgs before application of the spheroid-combined stimulating therapy to 57.2kgs 12 weeks after application, no significant but 4.51% change. Second, body fats of the experimental group changed from 18.8kgs before application to 16.4kgs 12 weeks after application, no significant but 12.77% reduction. Third, % fats of the experimental group decreased from 31.2% before application to 28.6% 12 weeks after application, no significant change but 8.33% reduction. Fourth, body mass index(BMI) of the experimental group decreased from 23.23 kg/㎡ before application of the therapy to 21.43kg/㎡ after application, no significant but 8.03% reduction. As seen above, the 12-week application of the spheroid-combined stimulating therapy produced no significant changes in physical constitution as physical exercise did, but improved physical constitution by reducing certain amount of boy weight, body fat, % fat and BMI. In order to detect more positive and significant changes in physical constitutions, continued studies and clinical experiments should be performed to more diverse subjects using technically reinforced equipment for spheroid-combined stimulating therapy.