발표논문초록 : 포스터 발표 ; GA3 가 작약 (芍藥) ( Paeonia lactiflora Pall . ) 의 종자와 미숙배의 (未熟胚) 발아에 미치는 영향

신종희,손재근,김재철,박소득 한국식물생명공학회 ( 구 한국식물조직배양학회 ) 1996 한국식물생명공학회 춘계학술연구발표회 Vol.1996 No.-

항진균제 감수성 검사의 최신지견: 검사법과 임상적 이용

신종희 대한임상미생물학회 2009 Annals of clinical microbiology Vol.12 No.4

During the past two decades, Clinical and Laboratory Standards Institute (CLSI) antifungal susceptibility testing methods for both yeasts and molds have been developed and established in response to increasing invasive fungal infections and the release of multiple new antifungal agents. In addition, other methods including Etest, the disk diffusion test, and some CLSI modification methods have been intensively studied. Antifungal susceptibility tests are now routinely used for local epidemiological surveys to determine the susceptibility patterns of clinical isolates of fungi, the degree of antifungal activity of newly developed antifungal agents, and to predict the clinical outcomes of antifungal therapy for patients with Candida infections. It is anticipated that in the near future, antifungal susceptibility tests that can detect amphotericin B resistance, that can be used to establish the minimum inhibitory concentration (MIC) breakpoints of molds, and that can provide increased clinical guidance for antifungal therapy, will be developed. This review focuses on the various methods used for antifungal susceptibility testing and the clinical utility of antifungal susceptibility testing.

Candida orthopsilosis에 의한 치명적 진균혈증 1예

신종희,최현정,신명근,서순팔,양동욱,박경화 대한임상미생물학회 2010 Annals of clinical microbiology Vol.13 No.3

Candida orthopsilosis is a recently described Candida species phenotypically indistinguishable from Candida parapsilosis. This new species can be identified only by using molecular methods. We describe here a fatal case of fungemia caused by C. orthopsilosis in a 75-year-old male patient who had panperitonitis after total gastrectomy with Roux-en-Y esophagojejunostomy. All 18 blood cultures obtained from admission day 16 to day 68 yielded the same Candida species. Both Vitek 2 (bioMerieux, Inc., Hazelwood,MO, USA) and API 20C (bioMerieux, Marcy-l'Etoile,France) failed to identify these isolates. However,DNA sequencing analysis of both D1/D2 domain and internal transcribed spacer region of rDNA showed 100% identity with C. orthopsilosis. The fungemia was persistent over 50 days despite of systemic antifungal therapy including fluconazole and caspofungin,and the patient expired on day 73 of his hospital stay. This represents the first reported case of fatal fungemia by C. orthopsilosis in Korea. (Korean J Clin Microbiol 2010;13:140-143)

Acinetobacter baumannii의 Plasmid DNA양상

신종희,서순팔,양동욱 대한임상병리학회 1994 대한임상병리학회지 Vol.14 No.2

Exophiala (Wangiella) dermatitidis에 의한 진균혈증 1예

신종희,정은선,신명근,서순팔,양동욱 대한임상미생물학회 2010 Annals of clinical microbiology Vol.13 No.3

We report a rare case of fungemia due to Exophiala (Wangiella) dermatitidis in a 4-month-old female infant who was admitted to an intensive care unit with sudden infant death syndrome (SIDS). E. dermatitidiswas repeatedly isolated from blood cultures (on the 28th and 32nd day of hospitalization) of the patient,who died on the 44th day of hospitalization. The fungus was identified by its morphological characteristics and DNA sequencing of both the D1/D2domain and the ITS region of rDNA. To our knowledge,this is the first reported case of E. dermatitidis fungemia in Korea. (Korean J Clin Microbiol 2010;13:135-139)

영남내륙 지역 과거 20년간 기후와 벼 조만성별 쌀 수량 변화

신종희,한채민,권중배,김상국 한국작물학회 2019 한국작물학회지 Vol.64 No.3

ABSTRACT The aim of this study was to analyze the relationship between rice yield and climate elements in Daegu (southern plain area) and Andong (inland mountainous area) regions. Over the past 20 years, rice yield has increased in both regions. The rice yield of middle and mid-late maturing cultivars in the recent 5 years increased by about 10% and 18%, respectively, compared to that produced in the early 2000s in the Daegu region. In the Andong region, the rice yield of mid-late maturing rice cultivars in the recent 5 years was higher by about 7% than that of the early 2000s. The number of panicles per hill and grain ripening rate significantly affected rice yield in mid-late maturing cultivars. In addition, the grain weight and grain ripening rate significantly affected rice production in middle maturing cultivars grown in the Daegu region. With regard to the middle maturing cultivars, the relationship between grain weight and rice yield had a positive significant correlation in both regions. To understand the effect of climate factors on rice yield, the milled rice yield of several rice cultivars produced over the past 20 years (1999-2018) at both locations, Daegu and Andong, were evaluated. The rice yields increased owing to long sunshine duration during the grain filling stage in the Daegu region. In Andong, rising maximum temperature during the vegetative stage increased rice yield of early and mid-late maturing cultivars. Long sunshine hours increased yield of mid-late maturing cultivars in both regions. 본 연구는 영남내륙에 속하는 대구 및 안동 지역의 벼 수 량에 영향을 미치는 수량구성요소와 기상요소를 파악하고 자 하였다. 과거 20년간 이들 지역의 기상변화를 분석한 결 과 벼 재배기간 중 평균기온은 대구지역보다 안동지역에서 +0.9°C/10년으로 2배 이상의 상승폭을 보였다. 이는 생식 생장기의 기온상승이 주요 원인으로 분석되었다. 강수량은 감소한 반면 누적 일조시수는 현저하게 증가하였는데 특히 안동지역의 일조시수 증가 폭이 컸다. 대구의 쌀 수량성은 꾸준히 증가하여 2000년 초반 5년 평균 수량보다 최근 5년 간 평균수량이 중만생종의 경우 18% 정도 현저한 수량 증 가를 보였다. 벼 수량과 수량구성요소의 관계를 분석한 결과 대구지역 중생종의 수량 증가는 현미천립중과 등숙율의 증가가 주요 요인으로 작용하였으며, 중만생종의 경우 포기당 이삭수, 등숙율 및 제현율의 증가가 쌀 수량 증가에 영향을 미친 것 으로 분석되었다. 안동의 경우는 중생, 중만생종에서 천립 중의 증가가 수량증가의 요인으로 분석되었다. 벼 수량과 기후요소와의 상관관계를 분석한 결과 대구지역에서 재배 된 중만생종 벼의 쌀 수량은 등숙기의 일조시수와 높은 상 관을 보였다. 안동지역 중만생종은 영양생장기 최고기온과 생육전반 일조시수의 증가가 수량에 유의한 영향을 미치는 것으로 판단되었다.

경상북도 지역별 최고품질 벼 품종의 수량 및 품질 특성

신종희,김상국,김세종 한국작물학회 2017 한국작물학회지 Vol.62 No.4

Rice quality should be considered as a primary consumer requirement. Regarding marketing, characteristics such as appearance, physicochemical properties, and palatability of brand rice are of high economic importance. Therefore, this study was conducted to select the optimal rice cultivars representing the commercial rice brands of Gyeongsangbuk-do province in Korea. Various rice grain quality components, grain appearance, several physicochemical properties of rice grain, and texture or palatability of cooked rice grains of ten cultivars (namely ‘top quality rice’) cultivated at three different locations, such as inland mountainous and southern plain areas of Gyeongsangbuk-do province in 2013~2014, were evaluated, and the obtained data was analyzed. ‘Hiami’ showed slightly higher protein contents and lower palatability of cooked rice than the other rice cultivars. Rice production and head rice yield produced at Gumi were the highest. The protein content of milled rice produced at Andong, an inland mountainous region, was approximately 0.3% point lower than that from other locations, whereas the amylose content of milled rice was approximately 1% point higher than those from the other plain regions, Daegu and Gumi. We evaluated the texture, Glossiness value determined using a Toyo teste meter and palatability of cooked rice of ten cultivars. The hardness of cooked rice produced in Andong was slightly lower than that produced in Daegu and Gumi, and additionally, the palatability of cooked rice produced in Andong was the best, followed by that producted in Gumi and Daegu. Considering rice yield and grain quality in the major rice cultivation areas of Gyeongsangbuk-do province, the rice cultivars that may be suitable for each region could be recommended mid-late maturation: ‘Younghojinmi’ and ‘Mipum’ in Daegu, ‘Daebo’, ‘Samgwang’, Chilbo’ and ‘Younghojinmi’ in Gumi, ‘Samgwang’, ‘Jinsumi’ and ‘Sukwang’ in Andong. These results obtained in this study imply that the selected cultivars with high yield and quality could be recommended with high priority to rice farmers in the regions. 2013~2014년간 벼 등숙기 기상은 평균기온은 평년대비±0.2℃ 로 비슷하고, 등숙 후반기인 10월 상순의 기온이 평년에 비해 지역별로 1.0~1.6℃ 높아 안정적인 등숙이 가능한 조건이었다. 지역별 출수기의 경우 대구, 구미지역보다5일 일찍 이앙한 안동지역의 평균 출수기가 2일 정도 늦어지는 경향이었으며, 영호진미의 경우 안동지역에서 출수기가 타 지역 보다 4~5일 더 늦어지면서 등숙률이 77.4%로현저히 낮았다. 구미지역의 쌀 수량이 평균 671 kg/10a로 가장 많았다. 수광의 경우 수당립수, 현미천립중, 등숙률, 쌀수량 등 전반적인 생육 및 수량특성이 지역별 차이 없이비슷하였다. 도정한 쌀의 완전미 비율은 대구지역에서는 진수미, 영호진미, 미품이 각각 92.0, 92.8, 90.0%로 높고 구미지역에서는 대보, 칠보, 수광이 90% 이상의 완전미율을 보였으며, 안동지역은 진수미와 수광의 완전미율이 높게 나타났다. 호품은 시험지역 모두에서 가장 낮은 완전미율을 나타내었다. 백미에 함유된 단백질 분석결과, 품종별로는 하이아미가 가장 높게 나타났으며 지역별로는 안동지방에서 재배된경우 모든 품종에서 단백질 함량이 낮았다. 특히 영호진미의 경우 대구, 구미, 안동지역 모두에서 6%이하의 단백질 함량을 나타내었다. 아밀로스 함량은 안동지역에서 재배된경우 대구와 구미지역에 비해 높았다. 고품과 대보의 경우대구지역에서 찰기와 부착성이 낮게 나타났고, 진수미와영호진미의 경우 재배 지역에 따른 식감 특성 차이가 적었다. 지역별 최고품질벼 수량 및 품질 특성 분석을 통하여대구지역은 진수미, 영호진미, 미품, 수광을 선발하고, 구미지역은 대보, 삼광, 칠보, 수광, 영호진미, 미품을, 안동지역은 삼광, 대보, 진수미, 수광을 선발 하였다. 이미 개발된 많은 우수한 품종의 특성을 파악하고 각 지역의 환경에 적합한 품종 선택하여 재배함으로써 고품질 쌀을 생산하면 농업인의 소득도 보장이 되고 우리 쌀도 지킬 수 있을 것이다. 따라서 본 시험결과를 활용하여 경북 주요 농업지대별식미가 우수한 품종을 선별하여 농가에서 지역브랜드로 활용토록 함과 동시에 지역별로 다른 환경변이에 맞추어 식미가 우수한 쌀을 생산 할 수 있도록 하는 자료를 제공하고자 한다.

한국인의 임상분리 아스페르길루스 균주에 대한 보리코나졸의 생체외 감수성

신종희,박미라,송정원,신동현,정숙인,박영규,신명근,조덕,기승정,서순팔,양동욱 대한의진균학회 2004 대한의진균학회지 Vol.9 No.3

Background: Voriconazole is a potent new triazole antifungal agent expected to be particularly useful for the treatment of invasive aspergillosis. However, in vitro susceptibility of voriconazole for clinical strains of Aspergillus species isolated in Korea has not been fully surveyed. Objective: We determined minimum inhibitory concentrations (MICs) of voriconazole for clinical Aspergillus isolates. Methods: A total of 100 clinical isolates of Aspergillus species (40 A. fumigatus, 24 A. flavus, 17 A. niger, 17 A. terreus and 2 A. nidulans) was tested. In vitro voriconazole susceptibility testing was accomplished utilizing the National Committee for Clinical Laboratory Standards (NCCLS) broth microdilution method M38-A. MIC of voriconazole was determined using RPMI medium at 48 h of incubation. Results: Among the 100 isolates of Aspergillus species tested, 98% were inhibited by ≤ 1 μg/mL of voriconazole. The MICs of voriconazole ranged from 0.125 to 2 μg/mL (geometric mean MIC, 0.52 μg/mL). The MIC50 (MIC at which 50% of the isolates tested were inhibited) and MIC90 were 0.5 and 1.0 μg/mL for all Aspergillus species, respectively. The strains showing MIC≥2 μg/mL were 0/40 (0%) in A. fumigatus, 1/24 (4%) in A. flavus, 1/17 (6%) in A. niger, 0/17 (0%) in A. terreus, and 0/2 (0%) in A. nidulans. Conclusion: These data demonstrate promising in-vitro activity of voriconazole against clinical strains of Aspergillus species isolated from Korean patients.

임상미생물학의 진전

신종희 주식회사 녹십자 2003 녹십자의보 Vol.31 No.5

주요 효모균과 사상형 진균의 항진균제 내성현황

신종희 대한감염학회 2009 Infection and Chemotherapy Vol.41 No.2

The incidence of invasive fungal infection has increased worldwide along with the increasing population of high risk patients. Recently developed antifungal agents, such as second-generation triazoles and echinocandins, provide the potential to improve therapeutic options against invasive fungal infections. However, treatment of invasive fungal infections has been hampered by both intrinsic and acquired resistance to antifungal agents among many fungal pathogens. A better understanding of the clinical impact of antifungal resistance is important for selecting the proper antifungal agent in patients with systemic fungal infections. This article reviews the current situation with regard to intrinsic and acquired resistance of clinically significant yeasts and filamentous fungi against currently available antifungal agents.