국내에서 생장한 Kenaf의 해부학적 특성

권영만,황원중,권성민,조준형,이명구,김남훈 江原大學校 森林科學硏究所 2005 Journal of Forest Science Vol.21 No.-

국내에서 재배한 kenaf의 해부학적 특성을 광학현미경법으로 조사하였다. Kenaf의 수피부에는 인피섬유, 사부방사조직, 피층유세포 등이 존해하였고, 목질부는 도관, 목섬유, 방사조직 등으로 구성되어 있었다. 도관은 고립관공과 2~3개가 방사방향으로 복합한 복합관공이 많이 존재하였고, 방사조직은 평복, 직립, 방형세포가 모두 분포하고 있었다. 접선단면에서 방사조직은 단열 및 다열방사조직으로 구성되었다. 수피비의 인피섬유층은 생장기간과 함께 증가하였다. Anatomical properties of Kenaf cultivated in Korea was investigated using light microscopy. Bast fiber, phloem ray and cortex parencyma cell were observed in bast, and vessel, wood fiber and rat in core. A lot of solitary and multiple radial pores in core existed. The cell type of ray parenchyma in radial section was procumbent, upright and square sell. Uniseriate and multiseriate rays existed in tangential section. The layer of bast fiber in bast increased with in creasing the growth period.

만성 심부전환자의 혈액에서 측정한 Tumor necrosis factor-α 및 Interleukin-6의 임상적 의의

김명구,김성구,박상호,한대희,강진환,변정득,심규혁,최병조,방덕원,온영근,현민수,권영주 순천향의학연구소 2001 Journal of Soonchunhyang Medical Science Vol.7 No.2

Background and objectives: Many conditions are responsible for the pathophysiology and progressive mechanisms of congestive heart failure. More recently, it has also become evidence that another class of biologycally activated molecules generically reffered to as cytokine these are also over expressed in congestive heart failure. Therefore, the purpose of this study was to measure concentrations of tumor necrosis factor-α(TNF-α), interleukin-6(IL-6) in mild to severe symptoms of heart failure and compare their values with those found in normal control and analysed correlation relationship between cytokine level, clinical findings and hemodynamic indicies. Subjects and Methodology: Levels of TNF-α and IL-6 were measured on pulmonary artery during cardiac catheterization in heart failure patients(n=32) and normal subjects(n=8) as well as physical examination and echocardiogram. Cytokines assay were performed on plasma using commercially available ELISA(Enazyme-Linked Immunosorbent Assay) kits. Results: Although the levels of TNF-α and IL-6 tend to increase in congestive heart failure group, the cytokines level was not made significantly statistical difference between congestive heart failure group and controls. When analyzing the correlation between the levels of PCWP(pulmonary capillary wedge pressure) and cytokines(TNF-α, IL-6), respectively, there were statistically significant correlation coefficient 0.32,(p<0.05), 0.39(p<0.01). The cytokine IL-6 and pressure of pulmonary artery were significant correlation.(correlation coefficient 0.36, p<0.02) More significantly, there was correlated with TNF-α and IL-6.(correlation coefficient 0.57, p<0.001) Conclusions: There was tended toward high concentration of TNF-α & IL-6 in congestive heart failure and significant difference for PCWP between TNF-α & IL-6, thus may be correlated with development and progression in congestive heart failure.

국립공원 계룡산 이용자의 행태와 만족도에 관한 연구

오두영,이권구 公州大學校 資源科學硏究所 1996 資源科學硏究論文集 Vol.4 No.-

The purpose of this theses is to examine the function of the National Park as a recreation place and the degree of visitors' satisfaction at the environment including the facilities in National Park by analyzing the important factors having an effect on them, and to show the plan for developing and managing the National Park. In the course of this study, the area of survey was selected Mt. Kyeryong National Parks Kongju shi Chung Chong Nam Do. The main method of data collection was interviews and questions and to collect data, a total of respondents was 322, sampled as final subjects of survey. The number of question in the questionnaire summed to 58, including 23 general questions and 35question on evalution after visiting park. The independent variables were divided into two;individual attributes(sex, age, job, schcooling, etc. 4questions) and a conductive variables (frequency of visiting,way of transport, from of company, length of in park, etc. 19 questions0 and the dependent variables were the satisfaction of landscape, the road and the facilities and inclusive satisfaction. The cronbach's alphas program was used to measure the reliability of Likert Scales. The cronbach;coefficient alphas all the categories of competences were 0.91. The analysis of data was used SPSS PC+ statistical analysis program in IBM 486 computer and was applicate such as a statistical methods. Also, a statistical level of sampling connective factor was frequency. T-test, multiple-regression analysis with a 01 significance level. The result od this study were as follows; 1.As for the respondents' individual attributes, the sex of respondents was mainly female, their age was mainly twenties, their schooling was mainly higher than that of secondary school graduates, their job was mainly student and housewife. 2.As for the respondents conduct a place of residence was mainly Tae-Jeon (69.9%), the means of transportation was mainly taking a regular bus (51.6%), a form of company was mainly friend or lover (49.4%), a visiting season was autumn(51.9%), and method of solving meal was to uss restaurants(48.4%). As for the number of using park for a year, visiting at any time were in large numbers(31.1%), but judging from the time staying in park (less than five hours user (59.6%) one day user(26.1%)), we could know that it was one day visiting type. 3.As for the feature of user's visiting purpose, the male was likery to take a walk or to climb, the female was likely to take a rest, to look at the sight and to date. As for the age, those more than forties were to sihtseeing tour, thirties∼forties were to take a walk or to climb, twenties∼thirties who want to date with friend or lover were mainly in large numbers. According to the job, anong the visiting purpose, those who has the purpose of taking a walk or climbing were office worker, service worker, student. Those who take the purpose of looking at the sight, going on a picnic with one's family were housewives, those who has the purpose of dating were mainly students. Compared with one's schooling, regardless of schooling, taking a walk, climbing and sightseeing are comparatively in large numbers, and most of students were for dating with their friends or lovers. 4.The primary factors that had on effect on satisfaction after using the natyre are the beauty of nature, mountain, valley, the harmony of tree, and change of season. But a variety of tree and a feature of sight present a less satisfaction, compared with others factor. 5.The primary factors that decide the satisfaction of using the road are a system of guidence, the width of road and paking materials. And according to being or not being the guidance at the place where many peole visit, it was mainly affected. And an ill-matched packing with natyre (pavement, concrete, block of a footpath) was not good. 6.The primary factors of satisfaction of using the feature were mainly the location and number of waste basket and dumping ground, the number of convenient facilities. Especially a confusion of use has an great effect on it. 7.The primary factors of satisfaction of aim of visiting park are mainly taking a walk, climbing, sightseeing, and dating. But compared with other factor, both viewing cultural inheritance and observation natyre pressent low satisfaction.

마이크웨이브 추출조건에 따른 참취 추출물의 총 폴리페놀 함량 및 항산화작용의 변화

김현구,권영주,김영언,남궁배 한국식품저장유통학회 ( 구 한국농산물저장유통학회 ) 2004 한국식품저장유통학회지 Vol.11 No.1

마이크로웨이브 추출방법과 환류 냉각 추출방법을 비교한 결과, 물과 에탄올의 혼합용매로 추출한 경우 마이크로웨이브 추출 방법에 의하여 추출시간을 단축시키면서 환류 냉각 추출 방법에서와 같은 수준의 가용성 고형분 및 총 폴리페놀 함량을 갖는 참취 추출물을 얻을 수 있었다. 마이크로웨이브 추출시 최적 마이크로웨이브 에너지는 120∼150 W 였고 추출시간은 4∼8분이 적당하였다. 추출에 사용한 용매들 가운데 에탄올, 메탄올 보다 물 그리고 물과 에탄올 또는 메탄올 혼합용매를 사용한 추출물의 가용성 고형분, 총 폴리페놀 함량 및 항산화 효과가 높은 것으로 나타났다. This study was conducted in order to compare reflux extraction(RE) and microwave-assisted extraction(MAE) in extraction efficiency and establish optimum microwave extraction condition in obtaining Aster scaber Thunb extracts. Extraction time was reduced considerably in MAD. When 70% methanol, 50% methanol, 70% ethanol, or 50% ethanol was used, no difference was found in the amount of soluble solid and total phenol between MAE and RE. The optimum microwave-assisted extraction conditions for Aster scaber Thunb were achieved by 120~150 watts of microwave energy and 4~8 minutes of extraction time. No signigicant changes were found in antioxidant activity using DPPH scavenging method over the variation of microwave power or extraction time. The use of diluted methanol or ethanol improved soluble solid content(30.8%), total polyphenol content(2.9%) and antioxidant activity(69%).

허혈성 심질환에서 염증지표에 관한 연구

번정득,권영주,박상호,한대희,이상철,강진환,김명구,심규혁,최병조,온영근,현민수,김성구 순천향의학연구소 2001 Journal of Soonchunhyang Medical Science Vol.7 No.2

Background : Atherosclerosis is the chief underlying cause of ischemic heart disease and there is increasing evidence that inflammation is an important determinant of the development of atherosclerosis. We assessed the levels of inflammatory markers in patients with ischemic heart disease and normal group who has normal coronary angiograms. Materials and Methods : Coronary angiography was performed in 142 patients. 107 patients of ischemic heart disease(stable angina pectoris 58, unstable angina pectoris 30, Acute myocardial infarction 19) and 38 normal control subjects. We assessed the level of inflammatory markers, such as CRP, ESR, fibrinogen and leukocyte. Results : CRP, ESR and fibrinogen values of the patients with stable angina pectoris and unstable angina pectoris were higher than that of normal control group, but there were no statistical significance. Leukocyte value of the patients with unstable angina pectoris(9003.3±701.5/mm^(3)) was significantly higher than that of the patients with stable angina pectoris(6685.5±245.8/mm^(3)) and normal control subjects(6394.3±235.1/mm^(3)). CRP, ESR and fibrinogen values of the patients with acute myocardial infarction were also higher than that of normal control subjects. CRP was 3.88±2.05 mg/dL in acute mocardial infarction group, and 0.29±0.15 mg/dL in normal control subject group(p<0.05). Fibrinogen was 541.6±45.1 mg/dL in acute myocardial infarction group, 321.4±25.6 mg/dL in normal control subject group(p<0.05). Leukocyte was 10942.1±737.6/mm^(3) in acute myocardial infarction group, 6394.3±235.1/mm^(3) in normal control subject group(p<0.05). Conclusions : This study demonstrate that CRP, fibrinogen and leukocyte values of acute myocardial infarction group were significantly higher than that of control group and stable, unstable argina pectoris group. Leucokyte values were significantly elevated in unstable angina group, but CRP values were not in unstable angina group.

관상동맥질환 환자에서 스텐트 시술 후 재협착에 관한 연구

강진환,권영주,박상호,한대희,이상철,김명구,변정득,최병조,심규혁,온영근,현민수,김성구 순천향의학연구소 2001 Journal of Soonchunhyang Medical Science Vol.7 No.2

Background and aims : In the coronary artery disease, a rate of restenosis was much decreased by placement of coronary stent than when percutaneous transluminal coronary angioplasty has been applied, but there are still important clinical problems of restenosis. There has been many progressive studies about various factors contributed to this restenosis. So, we studied a relation between restenosis after placement of coronary stent in the coronary artery disease and many clinical factors, characteristics of stenotic lesion and procedural factors, and also assessed the restenosis rate of various coronary stents. Methods : Total 58 lesions(46 cases of patients ; man 30, woman 16) were evaluated, which has been performed a follow-up coronary angiography after 6 months (mean 188 days) since coronary stent had been placed. Various stents were implanted and assessed a relation between restenosis and many factors. Results : Of 58 lesions were target stenotic studies, there were 22 of restenotic lesions(37.9%), and the restenosis rate wes statistically significant difference in the relation with diabetes mellitus(p<0.05), group of acute myocardial infarction(p<0.05) among clinical diagnosis of ischemic heart disease(stable angina, unstable angina and acute myocardial infarction) in clinical factors and with high dilation pressure of stent(p<0.05) in procedural factors. When the rate of restenosis was evaluated among implanted stents, it was lowest in the Multilink^(®) stent and highest in the Nir Royal^(®) stent(60%). Conclusion : In this study of restenosis and various factors after placement of stents in the coronary artety disease, factors such as diabetes mellitus, group of acute myocardial infarction and a dilation pressure of stent were significant related with restenosis and the restenosis rate of Multilink^(®) stent was lowest and the highest restenosis rate was of Nir Royal^(®) stent.

우울장애 환자에서 자살 시도와 트립토판 수산화 효소(Tryptophan Hydroxylase) A218C 유전자 다형성과의 관련성

심세훈,황보영,권영준,정희연,이분희,김용구 대한신경정신의학회 2008 신경정신의학 Vol.47 No.2

Objectives : Several lines of evidence suggest the serotonergic dysfunction involved in the biological susceptibility of suicide. Tryptophan hydroxylase (TPH), the rate-limiting enzyme in the biosynthesis of serotonin, Plays a vital role in serotonin metabolism. In a case-control study, we investigated whether the TPH gene was a susceptible factor for suicidal behavior in depressive patients. Methods : The subjects were 218 depressed patients who attempted suicide and visited emergency rooms in multi-medical centers. One hundred thirty hospitalized non-suicidal depressed patients and the 161 normal controls were matched with the suicidal group. Individuals in all 3 groups were evaluated independently by a Structured Clinical Interview for the purpose of establishing a DSM-IV criteria diagnosis (SCID). The severity of depressive symptoms was evaluated using Hamilton depression rating scale (HDRS). Results : There was no significant difference in genotype distributions and allele frequencies of TPH intron 7 A218C polymorphisms among 3 groups. Furthermore, no significant difference in genotype counts and allele frequencies of the polymorphisms was found among lethal suicidal depressed patients, non-suicidal depressed patients and the normal controls. Conclusion : This study suggests that the A218C polymorphism of the TPH gene is unlikely to have a major effect on the susceptibility of suicidal behaviors in depressive patients.

운동이 비만 여성의 심근산소소비량과 심전도 QTc 간격에 미치는 영향

이혁종,박윤정,김용권,한구석,진영수 대한스포츠의학회 1999 대한스포츠의학회지 Vol.17 No.1

The purpose of this study was to investigate the effects of exercise staining on MVO_2 and electrocardiogram QTc interval in obese woman and to propose the desirable method for weight loss. Subjects were fifteen obese women for this study. 8 subjects were prolonged QTc interval group and 7 subjects were normal QTc interval. They participated in exercise program consisted of aerobic exercise and weight training exercise at A Medical Center. The body composition including Boyd Weight, BMI, WHR and % Body Fat was measured. The resting and exercise(Bruce protocol 7th min) rate-pressure product(RPP) could be measured with HR and systolic BP for MVO_2 measurement. The electrocardiogram QTc interval was calculated with Bazzett's formula using automated software program. The measured data of this study were as follows: 1) After participating the exercise program consisted of aerobic exercise and weight training exercise, Body Weight, BMI, WHR and % Body Fat were significantly changed in two groups. (p<.05). 2. After participating in the exercise program consisted of aerobic exercise and weight training exercise, the resting and exercise MVO_2 using RPP were significantly changed in two groups(p<.05). 3) After participating in the exercise program consisted of aerobic exercise and weight training exercise, the electrocardiogram QTc interval were decreased but were not significantly changed in prolonged QTc interval group. (p<.29). However, it was significantly decreased in prolonged QTc interval(p<.00).

Genetic Characterization and Antimicrobial Susceptibility ofCampylobacterSpp. Isolated from Domestic and Imported Chicken Meats and Humans in Korea

Ku, Bok Kyung,Kim, Hae Ji,Lee, Young Ju,Kim, Young Ihl,Choi, Jung Su,Park, Mi Young,Kwon, Jin Wook,Nam, Hyang-Mi,Kim, Yong Hwan,Jung, Suk-Chan,Lee, Sun Jin,Kim, Sang Hyun,Kim, Jong Hyun Mary Ann Liebert 2011 Foodborne pathogens and disease Vol.8 No.3

Cellular Manipulation of Human Embryonic Stem Cells by TAT-PDX1 Protein Transduction

Kwon, Young Do,Oh, Sun Kyung,Kim, Hee Sun,Ku, Seung-Yup,Kim, Seok Hyun,Choi, Young Min,Moon, Shin Yong Elsevier 2005 Molecular therapy Vol.12 No.1

<P><B>Abstract</B></P><P>Human embryonic stem cells (hESCs) are an <I>in vitro</I> model system for the study of human early development and a potential source for cell-based therapies. An efficient strategy for cellular manipulation of hESCs may be highly valuable for the analysis of gene function involved in human embryogenesis and the development of cell-based therapies via induced differentiation into particular cell types. However, plasmid transfection of hESCs has low efficiency and viral transduction may not be the method of choice for cell-based therapies due to genome integration. To overcome these limitations, we applied protein transduction technology that can transfer proteins into cells via direct penetration across the lipid bilayer. Here, we show that the FITC dye fused to the TAT protein transduction domain (PTD) was efficiently transferred into hESCs. In addition, the PDX1 transcription factor, which plays a central role in pancreatic development, was transferred into hESCs as a fusion form of TAT PTD. The transduced TAT-PDX1 activated its downstream target genes and induced insulin protein production in hESCs. These results demonstrate that protein transduction could be used in the cellular manipulation of hESCs and would provide a significant breakthrough for basic and therapeutic research in hESCs.</P>