Isolation and Characterization of an Extremely Thermophilic Sulfur - metabolizing Bacterium from a Deep - sea Hydrothermal Vent System

KWAK,YI - SEONG,TETSUO KOBAYASHI,TERUHIKO AKIBA KOKI HORIKOSHI,YOUNG - BAE KIM 한국미생물생명공학회 1994 Journal of Microbiology and Biotechnology Vol.4 No.1

중복 원발암의 증례 1예: 위선암 및 방사선조사후 소장에서 발생한 혈관육종

김강 ( Kang Kim1 ),김선희 ( Sun Hee Kim ),문진창 ( Jin Chang Moon ),박윤지 ( Yoon Ji Park ),박진경 ( Jin Kyung Park ),서승영 ( Seung Young Seo ),정래영 ( Lae Young Jung ),임호영 ( Ho Young Yhim ),곽재용 ( Jae Yong Kwak ) 전북대학교 의과학연구소 2011 全北醫大論文集 Vol.35 No.1

Angiosarcomas are rare malignant neoplasms, which mainly occur on the skin and superficial tissue. Angiosarcoma in the small intestine after radiation therapy is very rare and there are less than 20 cases of small-intestine angiosarcoma after pelvic irradiation. We report a case of double cancer with gastric adenocarcinoma and postradiation angiosarcoma of the small intestine which has been diagnosed 20 years after pelvic irradiation therapy for uterine cervical cancer.

전자빔 물리기상 증착 코팅의 수학적 모델링에 관한 연구

곽은성(Eunsung Kwak),김용하(Yongha Kim),김기근(Keekeun Kim2) 한국추진공학회 2024 한국추진공학회 학술대회논문집 Vol.2024 No.11

유산소 운동능력 향상을 위한 중량물 부가 신발의 기능성 평가

곽창수 ( Chang Soo Kwak ),김희석 ( Hee Suk Kim ) 한국운동역학회 2004 한국운동역학회지 Vol.14 No.3

C. S. KWAK, H. S. KIM. The Evaluation of an additional Weight Shoe`s Function developed for the Improvement of Aerobic Capacity, Korean Journal of Sport Biomechanics, Vol. 14, No. 3, pp. 67-82, 2004. The purpose of this study was to evaluate the function and the safety of an additional weight shoe developed for the improvement of aerobic capacity, and to improve some problems found by subject`s test for an additional weight shoe. The subjects employed for this study were 10 college students. 4 video cameras, AMTI force platform and Pedar insole pressure distribution measurement device were used to analyze foot motions. The results of the study were as follows: 1. The initial achilles tendon angle and initial rearfoot pronation angle of an additional weight shoe during walking were 183.7 deg and 2.33 deg, respectively, and smaller than a barefoot condition. Maximum achilles tendon angle and the angular displacement of achilles tendon angle were 185.35 deg and 4.21 deg, respectively, and smaller than barefoot condition. Thus rearfoot stability variables were within the permission value for safety. 2. Maximal anterior posterior ground reaction force of additional weight shoe was appeared to be 1.01-1.2 B.W., and was bigger than a barefoot condition. The time to MAPGRF of an additional weight shoe was longer than a barefoot condition. Maximal vertical ground reaction force of additional weight shoe was appeared to be 2.3-2.7 B.W., and was bigger than a barefoot condition in propulsive force region. But A barefoot condition was bigger in braking force region. The time to MVGRF of an additional weight shoe was longer than a barefoot condition. 3. Regional peak pressure was bigger in medial region than in lateral region in contrast to conventional running shoes. The instant of regional peak pressure was M1-M2-M7-M4-M6-M5 -M3, and differed form conventional running shoes. Regional Impulse was shown to be abnormal patterns. There were no evidences that an additional weight shoe would have function and safety problems through the analysis of rearfoot control and ground reaction force during walking. However, There appeared to have small problem in pressure distribution. It was considered that it would be possible to redesign the inner geometry. This study could not find out safety on human body and exercise effects because of short term research period. Therefore long term study on subject`s test would be necessary in the future study.

Effects of β-Lapachone on Gastric Secretion

Yun-Bae Kim, Dongsun Park, In Geun Cho, Yun-Hui Yang, Jangbeen Kyung, Dajeong Kim, Ehn-Kyoung Choi, Tae Hwan Kwak3, Sang Ku Yoo 충북대학교 동물의학연구소 2011 Journal of Biomedical and Translational Research Vol.12 No.3

The effects of β-lapachone on gastric secretion were investigated. The pylorus of male Sprague-Dawley rats was ligated and intraduodenally injected with β-lapachone, and the volume, pH, free HCl, and total acidity of gastric fluid were measured 6 hours after the operation. Treatment with β-lapachone resulted in dose-dependent inhibition of gastric secretion Gastric fluid was reduced to 42.9% of control level by 100 mg/kg of β-lapachone, leading to an increase of pH to 6.70 from 1.85 in the control group. In parallel with the increase of pH, at this dosage, free HCl and total acidity decreased to 16.7% and 12.0%, respectively, of control levels. β-Lapachone exhibited ED50 values of 72, 46, and 47 mg/kg for inhibition of gastric volume, free HCl, and total acidity, respectively, implying a superior efficacy on gastric acid to volume. In comparison, pantoprazole (30 mg/kg) reduced the volume, free HCl and total acidity of gastric fluid to 53.0%, 26.0%, and 25.0%, respectively, of control levels, resulting in an increase in pH to 6.36. In the current study, it was confirmed that β-lapachone at an appropriate dose (100 mg/kg) exerted a higher inhibitory effect on gastric secretion than pantoprazole (30 mg/kg), a well-known proton-pump inhibitor. Therefore, it is suggested that β-lapachone could be a candidate compound for prevention or treatment of gastric ulcers induced by diverse psychological and physical stimuli.

Apoptotic Effect of Co-treatment with Chios Gum Mastic and HS-1200 on G361 Human Melanoma Cell Line

Young-Joo Hur,Young-Ki Kim,Hyun-Ho Kwak,Gyoo-Cheon Kim,Seung-Eun Lee,In-Ryoung Kim,Chul-Hoon Kim1,Bong-Soo Park 대한해부학회 2009 Anatomy & Cell Biology Vol.42 No.2

Chios gum mastic (CGM) is a resinous exudate obtained from the stem and the main leaves of Pistacia lenticulus tree native to Mediterranean areas. Recently it reported that CGM induce apoptosis in a few cancer cells in vitro. Bile acids and their synthetic derivatives induced apoptosis in various kinds of cancer cells and anticancer effects. It has been reported that the synthetic chenodeoxycholic acid (CDCA) derivatives showed apoptosis-inducing activity on various cancer cells in vitro. This study was undertaken to investigate the synergistic apoptotic effect of cotreatment with a natural product, CGM and a CDCA derivative, HS-1200 on G361 human melanoma cells. To investigate whether the co-treatment of CGM and HS-1200 compared with each single treatment efficiently reduced the viability of G361 cells, MTT assay was conducted. To investigate augmentation of apoptosis in G631 cells co-treated with CGM and HS-1200, DNA electrophoresis, Hoechst staining, proteasome activity assay, flow cytometry, Westen blot analyses, immunofluorescent staining and confocal microscopy were performed. In this study, G361 cells co-treated with CGM and HS-1200 showed several lines of apoptotic manifestation such as nuclear condensations, DNA fragmentation, the reduction of MMP and proteasome activity, the decrease of DNA content, the release of cytochrome c into cytosol, the translocation of AIF and DFF40 (CAD) onto nuclei, activation of caspase-9, caspase-3, PARP and DFF45 (ICAD), and up-regulation of Bax whereas each single treated G361 cells did not. Although the single treatment of 40 μg/mL CGM or 25 μM HS-1200 for 24 hrs did not induce apoptosis, the co-treatment of them induced prominently apoptosis. Therefore, combination therapy of CGM and HS-1200 could be considered, in the future, as an alternative therapeutic strategy for human melanoma.

왜 韓國人에는 冠狀動脈硬化性心臟病이 적은가?

李圭澤,權五鉉,金庚植,南相哲,金東洛,金相煜,郭然植,金聖倍,韓龍燮,金源准 慶北大學校 1960 論文集 Vol.4 No.-

A relationship between the amount of dietary fat, the concentration of serum lipids and the development of coronary heart disease has been one of the most important medical problems in the recent years. Accumulated data indicate that striking differences exist among different populations in the incidence and severity of coronary heart disease and these differences tend to correlate with dietary factors rather than with racial or other factors. Extensive studies on healthy peoples in various parts of the world lead to the conclusion that the incidence of coronary heart disease is directly to related the average level of serum cholesterol and this cholesterol level is directly related to the percentage of calories provided by fats in the diet, particularly common saturated fats of meats and dairy products. Such a study has never been carried out in Korea and studies in other countries of the Far· East have indicated that their diets are extremely low in the fat content and their serum cholesterol and frequency of the coronary heart disease are also remarkably low. In an attempt to correlate the dietary fat intake, blood lipid level and the Incidence of coronary heart disease in Korean people, the following population groups are selected. 319 farmers, 100 Taegu City people, 50 upper class people, 129 Buddhist Monks, and 90 Korean soldiers attached to the United States Army who have been fed American diet for from 1 to 18 months and 60 American soldiers and officers. On all of those people an analysis of dietary constituents, complete blood lipid analysis including total and free cholesterol, cholesterol ester, total esterified fatty acid . lipid phosphorus, neutral fat. C/P ratio and cholesterol ester/total cholesterol ratio and 12 leads electrocardiogram for evidences of the coronary heart disease were performed and the data thus obtained were analyzed and correlated. The most striking difference in diets of various groups is the fat content. The fat intake of Buddhist Monks and farmers is significantly lower than any other groups and their serum lipid level and the incidence of abnormal electrocardiogram are also lowest in the whole groups. Among Koreans upper class people of Taegu city have the highest fat intake and also the highest serum lipid level and the incidence of abnormal electrocardiogram. The dietary fat intake of Korean is significantly lower than that of American and the serum lipid level and the incidence of coronary heart disease are also significantly lower than American. The results of current study indicate that there is direct correlation between the dietary fat intake, the serum lipid level and the incidence of coronary heart disease and it seems to be reasonable to conclude that among many factors which may affect the remarkably low incidence of the coronary heart disease in Koreans the low dietary fat intake is the most important factor.

한국인에 있어서 상부식도의 이소성 위점막의 빈도 및 임상적 양상

이동욱(Dong Wook Lee),김은영(Eun Young Kim),박정모(Jung Mo Park),이원석(Won Suk Lee),곽동협(Dong Hyup Kwak),김정희(Jung Hee Kim) 대한내과학회 1998 대한내과학회지 Vol.54 No.1

Objectives: This study was performed to investigate the incidence and the clinical significance of inlet patch of heterotopic gastric mucosa in Koreans. This lesion can be found by close observation around the upper esophageal sphincter during upper gastrointestinal endoscopy. Methods: From March 1, 1996 to July 8, 1996, at Kwak's hospital, randomly selected 271 patients undergoing routine diagnostic upper gastrointestinal endoscopy were included in this study. Patients with the inlet patch were confirmed by histology. Clinical symptoms observed through medical history were recorded. Results: Twenty-six cases(9.6%) of the 271 patients studied had heterotopic gastric mucosa which was found at or just below the upper esophageal sphincter, varing from 3 to 30mm in diameter. It was velvety red in color and distinct from the surrounding normal squamous esophageal mucosa, resembling the Z-line at the esophagogastric junction. Parietal cells were identified in all 18 cases in which biopsy specimen contained deep glands, and chief cells were found in 14 eases. Histologically, fundic gland type was most frequent. The symptoms were relatively mild, 5 out of 6 patients who complained of throat discomfort were relieved by H2 antagonists. Conclusion: In Koreans the incidence of heterotopic gastric mucosa in the upper esophagus was similar to the reports from western countries unlike Barrett's esophagus, The etiology of these patches appeared to be congenital rather than acquired from reflux of gastric acid. This was supported by its location in the proximal esophagus and lack of correlation with reflux esophagitis. It must be considered as one of the differential diagnosis of a patient who complains of throat discomfort during diagnostic upper gastrointestinal endoscopy. Attention needs to be paid to find possible development of complications such as stricture and adenocarcinoma in patients with the inlet patch of heterotopic gastric mucosa.

Peripheral Blood NK Cells Reflect Changes in Decidual NK Cells in Women With Recurrent Miscarriages

Park, Dong Wook,Lee, Hyun Joo,Park, Chan Woo,Hong, Sung Ran,Kwak-Kim, Joanne,Yang, Kwang Moon Blackwell Publishing Ltd 2010 American journal of reproductive immunology Vol.63 No.2

<P><B>Citation</B> Park DW, Lee HJ, Park CW, Hong SR, Kwak-Kim J, Yang KM. Peripheral blood NK cells reflect changes in decidual NK cells in women with recurrent miscarriages. Am J Reprod Immunol 2010; 63: 173–180</P><P>Problem </P><P>We aimed to investigate if peripheral blood natural killer (pNK) cell levels are correlated with decidual NK (dNK) cell levels, and if chemokine expression has any role in dNK cell regulation.</P><P>Method of study </P><P>Decidual tissues of women having two or more miscarriages with normal karyotype were collected after miscarriage and an immuno-histochemisty study was made. pNK cells were evaluated using flow cytometric analysis.</P><P>Results </P><P>The %CD3<SUP>−</SUP>/56<SUP>+</SUP> and %CD3<SUP>−</SUP>/56<SUP>+</SUP>/16<SUP>+</SUP> pNK cells showed a significant correlation with mean number of CD56<SUP>+</SUP> dNK cells. The number of decidual CD16<SUP>+</SUP> cells was significantly higher in women with elevated pNK (≥15%) than that of normal pNK (<15%). The %CD3<SUP>−</SUP>/56<SUP>+</SUP> and %CD3<SUP>−</SUP>/56<SUP>+</SUP>/16<SUP>+</SUP> pNK cells showed an inverse correlation with duration of gestation. The CCL3<SUP>+</SUP> and CXCL12<SUP>+</SUP> cells were present in the decidua; however, staining intensity was not correlated with number of dNK cells.</P><P>Conclusion </P><P>The pNK cell levels reflect changes in dNK cell levels. This implicates that pNK cell level is a clinically useful marker to predict pregnancy outcome. Further study is needed to examine if elevated pNK cells enhance recruitment of dNK cells in the decidua.</P>

Optimal Condition of Fish Collagen Peptide-loaded Liposome Depending on Manufacture Scales

YunJung Lee,Eunji Kwak1,Honggyun Kim,Jingjing Bai,SangYoon Lee,Jung Gyu Lee,Dong Hyeon Park,Hee Jeong Choi,Junhwan Bae,Youngjae Cho,Mi-Jung Choi 한국식품영양과학회 2017 한국식품영양과학회 학술대회발표집 Vol.2016 No.10