총대장균군 측정의 정도관리에 적합한 균주의 조제

김주영,서은영,김미리,전남희,정현미,김명운,안태석,Kim, Ju-Young,Seo, Eun-Young,Kim, Mi-Ree,Jeon, Nam-Hui,Chung, Hyen-Mi,Kim, Myeong-Woon,Ahn, Tea-Seok 한국미생물학회 2008 미생물학회지 Vol.44 No.1

정도관리는 분석 결과의 신뢰성을 높이기 위해 필수적인 과정이며, 이를 위해서는 일정한 농도의 표준시료가 필요하다. 본 연구에서는 정도관리의 정략분석을 위해 실온에서도 일정시간 개체수가 유지되는 미생물 정도관리용 표준시료를 제작하였다. 대장균으로 조제한 미생물 시료에 세포 분열을 억제하기 위해 nalidixic acid ($100\;{\mu}g/ml$)와 cephalexin ($50\;{\mu}g/ml$)을 첨가하였다. 이후 12시간 간격으로 acridine orange로 염색 후 현미경으로 계수하는 방법과 한천 배지에 배양하여 집락을 계수하는 평판집락계수법으로 개체수 변화를 측정하였다. 현미경 계수 결과 초기간이 $3.7{\times}10^5\;cells/ml$이었을 때 대장균의 개체수는 48시간 동안 $3.5{\sim}4.2{\times}10^5\;cells/ml$의 범위를 보였고, 평판집락법 결과 초기값이 $1.2{\times}10^4\;CFU/ml$ 이었을 경우에는 $1.0{\sim}1.2{\times}10^4\;CFU/ml$로 조사되어 냉장 및 냉동의 필요 없이 실온에서도 48시간 동안 개체수가 비교적 안정적으로 유지되는 미생물 표준시료를 제작할 수 있었다. 따라서 본 연구에서 제작된 표준시료는 현재 상업적으로 판매되고 있는 표준균주 제품과 함께 정도관리의 정략분석을 위해 사용될 수 있을 것이다. Standard sample for quality control of total coliform measurement was procured by addition of nalidixic acid and cephalexin as bacteriostatic agents to Escherichia coli cultured broth. After making the standard sample, the number of E. coli was measured by fluorescence microscopic count method and plate count method by 12 hr interval. The numbers of E. coli remained unchanged for at least for 48 hr at room temperature which ranged from 3.5 to $4.2{\times}10^5\;cells/ml$ and from 1.0 to $1.2{\times}10^4\;CFU/ml$ by direct fluorescence microscopic count method and plate count method, respectively. This result suggests that microbial standard sample with bacteriostatic agents of nalidixic acid and cephalexin is usable for quantitative quality control.

Isolation and characterization of cellulolytic yeast belonging to Moesziomyces sp. from the gut of Grasshopper

김주영,정희영,박종석,조성진,이훈복,성기호,가야쓰리 수브라마니,김명겸,Kim, Ju-Young,Jung, Hee-Young,Park, Jong-Seok,Cho, Sung-Jin,Lee, Hoon Bok,Sung, Gi-Ho,Subramani, Gayathri,Kim, Myung Kyum The Microbiological Society of Korea 2019 미생물학회지 Vol.55 No.3

효모와 곤충 간의 집중적인 상호 작용은 곤충의 먹이에 대한 유인과 발달 및 행동에 대한 관련성을 보였다. 곤충 내장에서 분리된 효모는 먹이의 소화를 돕는 셀룰라아제(셀룰로오스 분해)를 분비한다. 한국의 경기도에서 수집한 메뚜기의 장에서 셀룰로오스를 분해하는 효모 세 균주를 분리 하였다. 효모 균주의 cellulase 활성을 확인하기 위해, 카르복시 메틸 셀룰로즈(CMC)를 함유하는 배지로 플레이트상의 투명한 영역을 요오드 용액을 사용하여 관찰하였다. 효모 $ON22^T$, $G10^T$ 및 $G15^T$ 균주는 CMC-플레이트 분석에서 양성 셀룰로오스 활성을 나타냈다. Large subunit rDNA 유전자와 Internal transcribed spacer (ITS) 영역의 D1/D2 영역의 서열 분석에 기초한 계통수를 통해 $ON22^T$와 $G10^T$ 균주가 Moesziomyces aphidis JCM 10318 (D1/D2 영역에서 각 100%와 99.8%, ITS 영역에서 각 98.4% 및 99.5% 서열유사성)와 가장 가깝고 G15는 Moesziomyces antarcticus JCM $10317^T$ 종 (D1/D2 영역에서 100%, ITS에서 100% 서열 유사성)에 속한다는 것을 밝혔다. 셀룰라아제는 바이오 에탄올 생산과 같은 바이오 연료와 같은 다양한 산업 공정에서 사용되고 있다. 따라서, 셀룰로오스 분해 미생물의 분리 및 연구는 중요성을 갖게 되었다. 이 논문은 한국의 Moesziomyces 속의 셀룰로오스 분해 효모 균주인 $ON22^T$, $G10^T$, $G15^T$에 대한 첫 번째 보고이다. An intensive interaction between yeasts and insects has highlighted their relevance for attraction to food and for the insect's development and behavior. Yeast associated in the gut of insects secretes cellulase which aided in the food digestion (cellulose degradation). Three strains of cellulose-degrading yeast were isolated from the gut of adult grasshoppers collected in Gyeonggi Province, South Korea. The strains $ON22^T$, $G10^T$, and $G15^T$, showed positive cellulolytic activity in the carboxymethyl cellulose (CMC)-plate assay. The phylogenetic tree based on sequence analysis of D1/D2 domains of the large subunit rRNA gene and the internal transcribed spacer (ITS) regions revealed that the strains $ON22^T$ (100 and 98.4% sequence similarities in D1/D2 domains and ITS) and $G10^T$ (99.8 and 99.5% in D1/D2 domain and ITS region) were most closely related to the species Moesziomyces aphidis JCM $10318^T$; $G15^T$ (100% in D1/D2 domains and ITS) belongs to the species Moesziomyces antarcticus JCM $10317^T$, respectively. Morphology and biochemical test results are provided in the species description. Cellulase with its massive applicability has been used in various industrial processes such as biofuels like bioethanol productions. Therefore, this is the first report of the cellulolytic yeast strains $ON22^T$, $G10^T$, and $G15^T$ related to the genus Moesziomyces in the family Ustilaginaceae (Ustilaginales), in Korea.

만성 복통과 발진을 주소로 내원한 6세 여아에서 진단된 폐흡충증 1예

김주영,박민규,이용주,허선,조기영,Kim, Ju Young,Park, Min Kyu,Lee, Yong Ju,Huh, Sun,Cho, Ky Young 대한소아감염학회 2018 Pediatric Infection and Vaccine Vol.25 No.1

Pleural paragonimiasis is uncommon in the pediatric population and therefore can be challenging to diagnose. This is a case of a 6-year-old girl with pleural effusion who had been having intermittent persistent epigastric pain and erythematous rash on the face, hands, and arms for 6 months. Exudative pleural effusion with prominent eosinophils and serum eosinophilia were observed. As patient showed high immunoglobulin M (IgM) titers against Mycoplasma pneumoniae, she was treated with antibiotics; however, the pleural effusion did not improve during hospitalization. Despite showing negative stool ova and cyst results, patient's serum and pleural effusion were positive for Paragonimus westermani-specific IgGs on enzyme-linked immunosorbent assay. Respiratory symptoms, pleural effusion, and skin symptoms improved after praziquantel treatment.

선박 항해통신장비 원격유지보수를 위한 데이터베이스 설계

김주영,옥경석,김주원,조익순,Kim, Ju-young,Ok, Kyeong-suk,Kim, Ju-won,Cho, Ik-soon 한국정보통신학회 2017 한국정보통신학회논문지 Vol.21 No.11

SOLAS 선박은 SFI group 코드 기준으로 적어도 83종의 장비를 탑재해야 하고, 각각의 장비는 다시 수 개에서 수십 개의 컴포넌트로 구성이 된다. 선박 운항 중에는 이러한 장비의 정상적인 운영이 보장되어야 하고, 문제 발생시 즉각적인 수리를 위하여 원격유지보수의 요구사항이 높다. 본 연구는 항해통신장비의 원격유지보수에 적합한 장비의 분류체계를 제시하고, 적절한 데이터베이스 구조를 도출하고자 했다. 연구 결과, 선박 항해통신장비의 원격유지보수를 위하여, 장비의 분류체계는 장비의 종류, 모델, 컴포넌트로 계층화되어야 하고, 고장모드와 영향분석, 질의응답, 수리이력, 상태정보와 예방정비 등의 테이블을 장비 모델 기준으로 구축하는 것이 효과적임을 알게 되었다. 140개의 항해통신장비 모델과 750개의 컴포넌트에 대하여 데이터베이스를 구축하였고, 실질적인 효과를 평가하기 위하여 선박장비 수리전문가가 클라우드 앱을 이용하여 검색 결과의 유용성을 평가하였다. The SOLAS ship should carry at least 83 different types of equipment based on the SFI group codes and each of which consists of several to dozens of components. During ship operation, it is necessary to ensure the normal operation of such equipment, and remote maintenance is highly demanded for immediate repair in the event of a equipment fault. This study proposes to find suitable classification system and to derive database structure for remote maintenance of navigation and communication equipment. As a result of this study, the classification system of equipment should be layered into equipment type, model, and component, and main table in the database consists of FMEA, service history, case data through Q&A, Preventive Maintenance. A database was constructed for 140 navigation and communication equipment models and 750 components. In order to evaluate the practical effects, service engineer evaluated the usefulness using the cloud app.

저강도 초음파 치료기기의 안전성 및 성능평가 가이드라인 수립을 위한 연구

김주영,김재영,노시철,최흥호,Kim, Ju-Young,Kim, Jae-Young,Noh, Si-Cheol,Choi, Heung-Ho 대한의용생체공학회 2015 의공학회지 Vol.36 No.5

In this study, we suggested the performance and safety testing guideline for low intensity pulsed ultrasound (LIPUS) represented by the ultrasound fracture treatment device and cartilage treatment device and low intensity focused ultrasound (LIFU) represented by ultrasonic face lifting device. For these study, the international standards and management regulations of Korea, Japan and United State were analyzed. And the usefulness and applicability were evaluated by testing with commercial equipment and reflecting the views of the industry and experts. As a result of this study, the safety and performance test guidelines for low intensity therapeutic ultrasound device were proposed by presenting the 10 items for LIPUS and 12 items for LIFU. The suggested guidelines are considered a high utilization in the domestic testing and approval authorities. And they are also thought to be useful to new technology development.

Cellulose degrading basidiomycetes yeast isolated from the gut of grasshopper in Korea

김주영,장준휘,박지현,정희영,박종석,조성진,이훈복,성기호,김명겸,Kim, Ju-Young,Jang, Jun Hwee,Park, Ji-Hyun,Jung, Hee-Young,Park, Jong-Seok,Cho, Sung-Jin,Lee, Hoon Bok,Limtong, Savitree,Subramani, Gayathri,Sung, Gi-Ho,Kim, Myung The Microbiological Society of Korea 2018 미생물학회지 Vol.54 No.4

메뚜기는 광합성으로 고정된 탄소의 소화에서 중요한 역할을 한다. 장내 미생물 군의 도움으로, 메뚜기는 셀룰로오스 및 헤미셀룰로오스와 같은 잎의 성분을 분해할 수 있다. 본 연구는 한국의 기도에서 수집한 메뚜기 껍질에서 추출한 셀룰로오스 분해 효모 균주를 조사하기 위해 이루어졌다. 효모 균주 중 ON2와 ON17 (두 균주)과 ON6 (한 균주)는 CMC-플레이트 분석에서 셀룰로오스 활성을 보였다. Large subunit rDNA의 D1/D2영역의 서열과 internal transcribed spacer (ITS) 영역의 분석 결과, ON2와 ON17 균주가 Papiliotrema aspenensis CBS $13867^T$와 가장 밀접하게 관련되어 있었고(D1/D2 영역의 서열 유사성은 100% ITS에서 99.4%의 서열 유사성) ON6 균주는 Saitozyma flava와 관련된(D1/D2영역에서 100%, ITS에서 99.0%) 밀접하게 관련이 있었다. 이 세 가지 효모 균주는 모두 셀룰로오스를 분해할 수 있으므로 공생하는 효모들은 탄수화물 분해를 위한 효소를 자체적으로 생산하고 당 단당체를 휘발성 지방산으로 전환시킬 수 있다. Tremellomycetes에 속하는 공생 효모 균주인 ON2, ON17 (Papilioterma 속)과 ON6 (Saitozyma속)은 한국에는 보고되지 않은 균주이다. Grasshoppers play vital role in the digestion of photosynthetically fixed carbons. With the aid of intestinal microflora, the grasshopper can degrade leaves constituents such as cellulose and hemicellulose. The purpose of this study was to examine cellulolytic yeast isolates from the gut of grasshoppers collected in Gyeonggi Province, South Korea. Among the yeast isolates, ON2, ON17 (two strains), and ON6 (one strain) showed positive cellulolytic activity in the CMC-plate assay. The sequence analyses of D1/D2 domains of the large subunit rDNA gene and the internal transcribed spacer (ITS) regions revealed that the strains ON2 and ON17 were most closely related to Papiliotrema aspenensis CBS $13867^T$ (100%, sequence similarity in D1/D2 domains; 99.4% sequence similarity in ITS) and strain ON6 related to Saitozyma flava (100% in D1/D2 domains; 99.0% in ITS). All these three yeast strains are capable of degrading cellulose; therefore, the members of endosymbiotic yeasts may produce their own enzymes for carbohydrate degradation and convert mobilized sugar monomers to volatile fatty acids. Thus, the endosymbiotic yeast strains ON2, ON17 (represents the genus Papilioterma) and ON6 (Saitozyma) belonging to the family Tremellomycetes, are unreported strains in Korea.

국제프랜차이징 연구요소 및 연구방향

김주영,임영균,심재덕,Kim, Ju-Young,Lim, Young-Kyun,Shim, Jae-Duck 한국마케팅과학회 2008 마케팅과학연구 Vol.18 No.4

The purpose of this research is to construct research framework for international franchising based on existing literature and to identify research components in the framework. Franchise can be defined as management styles that allow franchisee use various management assets of franchisor in order to make or sell product or service. It can be divided into product distribution franchise that is designed to sell products and business format franchise that is designed for running it as business whatever its form is. International franchising can be defined as a way of internationalization of franchisor to foreign country by providing its business format or package to franchisee of host country. International franchising is growing fast for last four decades but academic research on this is quite limited. Especially in Korea, research about international franchising is carried out on by case study format with single case or empirical study format with survey based on domestic franchise theory. Therefore, this paper tries to review existing literature on international franchising research, providing research framework, and then stimulating new research on this field. International franchising research components include motives and environmental factors for decision of expanding to international franchising, entrance modes and development plan for international franchising, contracts and management strategy of international franchising, and various performance measures from different perspectives. First, motives of international franchising are fee collection from franchisee. Also it provides easier way to expanding to foreign country. The other motives including increase total sales volume, occupying better strategic position, getting quality resources, and improving efficiency. Environmental factors that facilitating international franchising encompasses economic condition, trend, and legal or political factors in host and/or home countries. In addition, control power and risk management capability of franchisor plays critical role in successful franchising contract. Final decision to enter foreign country via franchising is determined by numerous factors like history, size, growth, competitiveness, management system, bonding capability, industry characteristics of franchisor. After deciding to enter into foreign country, franchisor needs to set entrance modes of international franchising. Within contractual mode, there are master franchising and area developing franchising, licensing, direct franchising, and joint venture. Theories about entrance mode selection contain concepts of efficiency, knowledge-based approach, competence-based approach, agent theory, and governance cost. The next step after entrance decision is operation strategy. Operation strategy starts with selecting a target city and a target country for franchising. In order to finding, screening targets, franchisor needs to collect information about candidates. Critical information includes brand patent, commercial laws, regulations, market conditions, country risk, and industry analysis. After selecting a target city in target country, franchisor needs to select franchisee, in other word, partner. The first important criteria for selecting partners are financial credibility and capability, possession of real estate. And cultural similarity and knowledge about franchisor and/or home country are also recognized as critical criteria. The most important element in operating strategy is legal document between franchisor and franchisee with home and host countries. Terms and conditions in legal documents give objective information about characteristics of franchising agreement for academic research. Legal documents have definitions of terminology, territory and exclusivity, agreement of term, initial fee, continuing fees, clearing currency, and rights about sub-franchising. Also, legal documents could have terms about softer elements like training program and operation

국내 건설 생산성 저해요인 분석 및 향상방안 제시

김주영,최재현,이승현,Kim, Ju-Young,Choi, Jae-Hyun,Lee, Seung-Hyun 대한건축학회 2011 대한건축학회논문집 Vol.27 No.2

A construction project is accomplished by the organized production with various and complicated activities. Productivity management for each activity is very important for completing the project successfully. In general, this work productivity is utilized as the index which shows the construction capacity of a construction company and affects to its profit ultimately. So, the improvement of work productivity through the effective field management is an important issue that should always be considered. In case of domestic construction industry, the terminology and concept of productivity seems to be very common, but its meaning is still regarded as ambiguous and inclusive. Specifically, most of researches relating to productivity are performed with an up-to-date keyword. However, the foundation studies such as the identification and analysis of productivity impact factors are actually insufficient. Therefore, this research identified and analyzed productivity loss factors which may occur on construction job sites. Also, it tried to suggest both the causes and solutions for productivity loss with the Delphi technique and reliability analysis. Based upon the research result, it is supposed to improve work productivity by acknowledging and making efforts to avoid potential risks associated with those identified factors early ahead of performing construction projects.

추적테이블을 이용한 요구사항 변경관리 및 추적 효과 연구

김주영,류성열,황만수,Kim, Ju-Young,Rhew, Sung-Yul,Hwang, Man-Su 한국정보처리학회 2010 정보처리학회논문지D Vol.17 No.4

소프트웨어 프로젝트의 실패요인 중 54%가 요구사항관리 미흡에서 발생하며, 이중 22%는 요구사항 변경관리에서 기인한다. 따라서 실패요인을 줄이기 위해 요구사항 관리활동이 중요하며 이중 요구사항 변경을 위한 핵심활동으로 추적기법을 활용한다. 추적기법에 사용되는 방법인 추적테이블은 단순링크 방식으로 사용이 간편하고 가독성이 있으며 추적의 정확성이 높은 장점이 있다. 그러나 기존 추적테이블 연구는 변경관리 방법 및 추적의 효과를 구체적으로 제시하고 있지 않으며, 또한 변경영향 추정연구는 추정방법이 복잡하여 실용성에 한계가 있다. 이에 본 연구는 기존에 연구된 추적테이블을 이용하여 변경요구사항을 관리하는 방법을 제시하고 이로 인해 변경율 및 변경영향도를 기존 연구에 비해 용이하게 추정할 수 있는 기법을 제안한다. 또한 15개 프로젝트를 대상으로 추적테이블의 효과를 가설 검정하여 추적테이블이 프로젝트 성공에 영향을 미치며, 요구사항 관리미흡으로 인한 실패요인을 감소시킴을 확인한다. Insufficient requirement management accounts for 54% ofunsuccessful software development projects and 22% of insufficient requirement management comes from requirement change management. Hence, requirement management activities are important to reduce failure rates and a tracing method is suggested as the major factor in requirements change management. A traceability table is easy to use because of its legibility accurate tracing. However, traceability tables of existing studies have failed to concretely suggest method of change management and effect of traceability. Also, studies of methods to estimate change impact is complex. Hence, this study suggests how to use a traceability table to manage changes in requirements. Together, in comparison to existing studies, this study suggests easier methods to estimate change rate and change impact. Also Fifteen projects were sampled to test the hypothesis that traceability table influences the success of projects and that it decreases the failure rate that comes from the insufficient requirements management.

석면노출연구를 위한 공간분석기법

김주영,강동묵,Kim, Ju-Young,Kang, Dong-Mug 한국환경보건학회 2012 한국환경보건학회지 Vol.38 No.5

Objectives: Spatial analysis is useful for understanding complicated causal relationships. This paper focuses trends and appling methods for spatial analysis associated with environmental asbestos exposure. Methods: Literature review and reflection of experience of authors were conducted to know academic background of spatial analysis, appling methods on epidemiology and asbestos exposure. Results: Spatial analysis based on spatial autocorrelation provides a variety of methods through which to conduct mapping, cluster analysis, diffusion, interpolation, and identification. Cause of disease occurrence can be investigated through spatial analysis. Appropriate methods can be applied according to contagiousness and continuity. Spatial analysis for asbestos exposure source is needed to study asbestos related diseases. Although a great amount of research has used spatial analysis to study exposure assessment and distribution of disease occurrence, these studies tend to focus on the construction of a thematic map without different forms of analysis. Recently, spatial analysis has been advanced by merging with web tools, mobile computing, statistical packages, social network analysis, and big data. Conclusions: Because the trend in spatial analysis has evolved from simple marking into a variety of forms of analyses, environmental researchers including asbestos exposure study are required to be aware of recent trends.