소 수정란의 공배양 및 동결이 체외발생에 미치는 영향에 관한 연구

이종진,이명헌,남윤이,이만휘,이봉구,김상근 충남대학교 수의과대학 동물의과학연구소 1996 動物醫科學硏究誌 Vol.4 No.-

The studies on the carried out to investigate the effects of co-culture with cumulus cells and oviduct epithelial cells on the in vitro fertilization and cleavage rate of bovine follicular oocytes, and to determine the optimum thawing temperature and equilibration time of frozen bovine embryos and on survival rate and in vitro developmental rate of bovine embryos. The ovaries were obtained from slaughtered Korean native cows. The follicular oocytes were cultured in TCM-199 medium containing 10 IU/㎖의 PMSG(Sigma, USA), 10 IU/㎖의 hCG, 1 ㎍/㎖의 β-estradiol(Sigma, USA) and 10% FCS(Sigma, USA) for 24-48 hrs in incubator with 5% CO_2 in air at 38.5℃ and then matured oocytes were again cultured for 12-18 hrs with motile capacitated sperm by preincubation of heparin. The bovine embryos following dehydration by cryoprotective agents and a various concentrations of sucrose were directly plunged into liquid nitrogen and thawed in 30℃ water. The results are summarized as followes : 1. The in vitro fertilization and in vitro developmental rates of bovine oocytes co-cultured with cumulus cells in TCM-199 media were 75.0%-76.8% and 17.3%-27.6%, respectively. And in-vitro fertilization rates of cumulus-enclosed oocytes(55.4%) were significantly(p<0.05) higher than cumulus-denuded oocytes(23.1%). 2. The in vitro fertilization and in vitro developmental rates of bovine oocytes co-cultured with 1 ×10^4 cells/㎖, 1 ×10^6 cells/㎖, 1 ×10^8 cells/㎖ and 1 ×10^15 cells/㎖ oviduct epithelial cells in TCM-199 media were 74.5%-77.8% and 15.7%-21.2%, respectively. 3. The in-vitro fertilization and in vitro developmental rates of bovine oocytes co-cultured in TCM-199 media containing PMSG, hCG, PMSG + hCG, PMSG + β-estradiol, hCG + β-estradiol 0 to 40 hrs after insemination were 74.0%-77.4% and 18.9% - 23.1, respectively. 4. The survival rates of bovine embryos thawed after rapid freezing in the media containing various kinds of cryoprotective agents added 0.25M and 0.50M sucrose were 14.7% - 35.1% and 17.6% - 31.6%, respectively. The survival rates of bovine embryos thawed after rapid freezing in the freezing media containing a various concentration of sucrose added 1.5M and 2.0M glycerol, 1.5M and 2.0M DMSO, and 1.5M and 2.0M propanediol were 23.5% -31.4% and 20.6% - 34.1%, respectively. 5. The temperature thawed at 30℃ after rapid freezing of bovine embryos resulted in a significantly higher embryos survival rate than did at 20℃ and 35℃. 6. The equilibration time on the survival rates of bovine embryos was attained after short period of time(2.5-5 min.) in the freezing medium higher than ling period of time(10-20min.).

위암 환자에게 세포성 면역 기능에 관한 연구

이재익,윤일국,이종선,김종완,장준,안정기,송민호,정현용,이헌영,김상용,김영건 忠南大學校 癌共同硏究所 1991 癌共同硏究所 硏究誌 Vol.1991 No.-

위암 환자에서 세포성 면역 기능에 관한 연구

이재익,윤일국,이종선,김종완,장준,안정기,송민호,정현용,이헌영,김삼용,김영건 충남대학교 암연구소 1991 癌共同硏究所 硏究誌 Vol.1 No.1

We performed a variety of lymphocyte stimulation tests, delayed hypersensitivity skin tests, and enumeration of several lymphocyte subpopulations in 21 patients with gastric cancer who did not receive any form of anticancer therapy, and in 20 healthy controls. The gastric cancer patients had significantly decreased number and total score of positive reactions in delayed hypersensitivity skin tests compared with the healthy controls. The percent of CD_(4) positive lymphocytes in the gastric cancer patients was significantly decreased compared to the normal control subjects, but it had no correlation with the total score of delayed cutaneous hypersensitivity reactions. The ratio of helper to suppressor cells was lower in the cancer group. The uptake of 3H-thymidine was markedly depressed in cancer patients when stimulated with various mitogens. There was little correlation between any of the stimulation tests and any of the lymphocyte subpopulation proportions to delayed hypersensitivity cutaneous reactions. Optimal proliferative response was found in lymphocytes stimulated with 10 micrograms of concanavalin-A and 10 microgram of phytohemagglutinin. Advanced stage patients had greatly depressed delayed hypersensitivity skin reactions and proliferative responses to mitogens compared with localized diseases. These results suggest that gastric cancer patients have depressed cellular immune functions, which mainly result from the decreased helper cells and defects in functional proliferative response to mitogens. Interleukin-2 and interferon-gamma restored the in vitro proliferative response of lymphocyte in patients with gastric cancer.

Gene pyramiding effect of two major bakanae disease resistance gene using qBK1 and qBKWD

Sais-Beul Lee,Tae-Heon Kim,Su-Min Jo,Jun-Hyun Cho,Ji-Yoon Lee,Jong-Hee Lee2,Dongjin Shin,You-Chun Song,Myung-Kyu Oh,Dong-Soo Park 한국작물학회 2017 한국작물학회 학술발표대회 논문집 Vol.2017 No.-

10주간의 체계적 운동이 노인들의 골밀도에 미치는 영향

이규문,최종환,김창범,김태헌 忠北大學校 平生體育硏究所 1997 平生體育硏究所 論文集 Vol.9 No.-

An experimental study was designed to investigate the effects of 10-weeks exercise on the bone mineral density (BMD) in women (ages 55 to 74). The subjects were placed into two age groups; middle aged group(n=19, 62.2±2.5 yrs) old group(n=16, 69±2.3 yrs). All subjects completed the bone mineral density test ( Radius and Ulna) by computed tomography both prior to and immediately following 12-weeks program. Both experimental groups participated in an 12-week exercise program consisting of three 80-min supervised exercise bouts a week. A pairs T-test was used to examine differences between pre-and post-test scores in the bone mineral density, depending on each age group. First the significant improvement (1.7%) appeared on BMD in all subjects together after 10-weeks exercise. Specifically, the middle aged group showed statistically significant increase(1.2%) between pre-and post-test scores in he bone mineral density after 10-weeks program. However, even though the improvement (2.2%) of BMD in the older group was not significant in statistics, the improvement(2.2%) of BMD in the older group is bigger than the improvement (1.2%) of BMD in the middle aged group. Therefore, the results suggest that improvements in BMD may be affected by acute exercise and may support the affective beneficence of exercise for older populations.

사춘기 여학생의 初潮와 體格요인간의 相關에 관한 연구

이종헌,전은정 대구효성가톨릭대학교 응용과학연구소 1998 응용과학연구논문집 Vol.6 No.2

The purpose of this study was to find the relationship between menarche and physique factor. The subjects of this study were randomly selected 275 Junior High school students who lived in Taegu and divided into four groups according to menarche. And height, weight, chest grith, relative body weight, relative chest grith, Rohrer's index, Koup's index, Vervaeck's index were used for the physique factor. The results were as follws, 1. It was shown that menarche of adolescent junior students was 12.8 years old. 2. It was shown that the growth condition of physique factor - height, weight, chest grith, relative body weight, Koup's index, Vervaeck's index - from 7 to 15 years old girls who had an earlier menarche group was superior to later menarche group (P<0.05). 3. It was shown that the growth condition of physique factor - relative chest grith, Rohrer's index - from 13 to 15 years old girls who had an earlier menarche group was superior to later menarche group (P<0.05). 4. At the 12 years old, the growth condition difference of physique factor who had an menarche group aged 12 is likely to bigger than menarche group aged 15. And, at the 12~13 years old, growth velocity of physique factor is most fast.

장례 풍습의 변천과 홍콩인의 의식변화

이헌종 한국인문과학회 2006 人文學論叢 Vol.5 No.2

This paper seeks to observe what alteration process traditional funeral customs of China in the urban Hong Kong. Hong Kong, went through with the economic development after the 1970s, and what form and adaptation process it went through after the 1977 regression in Hong Kong. In field researching, following the ethnographical research method, researches on interviews and various material culture information were executed concentrating on Yonghyang funeral hall one of the important funeral hall in Hong Kong. The funeral service of Hong Kong has changed into a new form gradually unifying various Chinese funeral customs in the process of becoming a modern city. This alteration in funeral service is related to the process of modernization, globalization and pluralistic development since the 1970s. The unification of Chinese and Western costumes, changes of alters and offerings, alterations in thoughts of cremation in the funeral process can be seen as revelations of multi-cultural aspect of Hong Kong stepping into the modern society. The decisive reason for the changes of funeral service in concentrating on such funeral hall can be found in the economic aspect. Even burial customs (cremation, memorial service) sustained by religious perspectives were gradually depending on vast growth of population, urbanization, and economic efficiency. The mental depression situation after and before 1997 in the whole society of Hong Kong brought in and out immense economic problems like reduction in investment, foreign investment, a crash in real estate markets, and vast growth in immigrants. Hong Kongers, who were afraid of the changes in life patterns toward Hong Kong through mainland's political intervention, even chose not only resistance but also a way to adapt. However, the previously made new tradition of package tradition concentrating on funeral service prevailed and settled as the funeral service of Hong Kong. Furthermore, the professionalism, modernism, and standardization of the method, with the economic efficiency, are influencing even the Chinese in mainland china. That is, for the current Hong Kong is a model China sought to make Shanghai into a modern city, if economic structure planned in that direction sustains, modernized forms of cultural pattern of Chinese tradition including funeral services, is one of China's ultimate future situations. These shows an aspect of the possibility of further acceleration in Hong Kong in China, where China in Hong Kong is emphasized. One of the oldest cultural phenomenons in burial methods, and known to sustain its tradition, shows that it possesses the cultural element that changes in the most accelerating speed in the modern society of economical efficiency and comfort. Although, the method and process of handling the dead shows different forms depending on societies and cultures, and has an changeable element according to time, it demonstrated changes can be accelerating depending on the quality of alteration. The shift in burial customs is possibly an adequate study subject when seeking to find the alteration progress of cultural phenomenon. As an aftermath of the 1997 Hong Kong regression, 10% of total population immigrated, an immense incident, yet not great enough stress for the Hong Kong citizens to return to the traditional funeral custom of China. Hence, unlike an incident of inevitably changing its memorial service to adapt to the economic changes, chosen for the necessity of groups of citizens, this was a Hong Kong like active cultural phenomenon. Instead, the various cultural urbanized phenomenon of current Hong Kong including the funeral customs, everyday Taoism, will become the future map enabling the prediction of yet to be modernized cities of mainland China.

SAS를 이용한 흡연요인 분석에 관한 연구

이종헌 대구효성가톨릭대학교 1995 연구논문집 Vol.50 No.1

Most people begin to smoke in their teenage years, emulating parents or celebrities who smoke. Teenagers also smoke to attain acceptance in their peer group. About half of the people who experiment with smoking continue the habit into adulthood. There must be some very compelling reasons that peo;oe continue to smoke despite the unpleasant taste, the initial adverse physiological reactions to smoke and nicotine, and the knowledge-now widespread-that tobacco smoke can cause serious, even life-threatening, illness. The reasnns peopoe give for smoking are many and varied. The motivation of smokers can be group into four general categories: 1. Pleasurable relaxation-X3,X9 2. Reducing negative feelings(crutch)-X4,X10,X16 3. Craving-X5,X17 4. Habit-X6,X12,X8

보드 레벨 플립칩의 4점 굽힘 시험 시 파단 특성 연구

이용성,정종설,이재헌,신기훈,정성균 한국공작기계학회 2008 한국공작기계학회 춘계학술대회논문집 Vol.2008 No.-

In general, circuit board assemblies experience various mechanical loadings during assembly and in actual use. The repeated cyclic bending can cause electrical failures due to circuit board cracks, solder interconnects cracks, and the component cracks. In this paper, we report on the failure characteristics of board level flip chips under the repeated cyclic bending. We first describe a new 4-point bending tester, which is developed according to JEDEC standard No.. 22B113. The performance of the tester is then estimated through actual experiments . Test results reveal that the cracks first occur on the outer balls around 20,000 cycles and gradually propagate to the inner balls where cracks are found around 70,000 cycles.

SOSM 方法에 의한 RC 短柱의 신뢰성 설계

李鍾憲 慶一大學校 1993 論文集 Vol.9 No.1

Reliability based design method of RC column is presneted by using Second Order Second Moment method, and applied to short tied column. The results of SOSM are compared to that of AFOSM. In the design of short column, the differences of the results of two methods are not so significant. The proposed algorithm can easely be used for reliability based design of rectangular RC tied column. So the proposed method is recomended for the design of RC columns, and it is expected that SOSM is prefer to AFOSM in case slender column design.