김치유래 젖산균의 균체지방산 분석을 이용한 분류학적 연구

이정숙,정민철,김우식,이근철,김홍중,박찬선,이헌주,주윤정,이근종,안종석,박완,박용하,민태익 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.2

표준균주를 포함한 230여개의 김치유래 젖산균에 대한 균체지방산(FAMEs)을 분석하였다. FAMEs profiles는 Euclidian Distance 17.5에 의해 7개의 Major Cluster와 1개의 Single Cluster로 나뉘어졌다. 이중 A, B, C 및 Cluster는 Leuconostoc속으로 분석되어졌고, F는 Lactobacillus속으로 분석되어졌다. 그리고 E와 G cluster는 두개의 Genus가 혼재되어 나타났으며 보충적인 연구가 필요하다. 앞으로 김치유래 젖산균의 균체지방산 분석결과를 기반으로 한 데이타베이스에 95가지 탄소원을 이용하는 수치분류학적 접근방법 및 Pyrolysis Mass Spectrometry 등의 화학적 분석 방법과 분자친화적 연구를 통한 종합적 분류정보 체계가 갖추어지면 젖산균의 신속, 정확한 동정 및 연구에 활발히 이용되어질 것이다. Two hundreds and thirty lactic acid bacteria, mostly isolated from Kimchi, including type strains were sued for analysis of cellular fatty acids. The 230 test strains were recoverd in 7 major and 1 single clusters defined a Euclidian distance of 17.5. These aggregate taxa were equivalent to the genus Leuconostoc (aggregate group A, B, C and D), and the genera Leuconostoc and Lactobacillus (aggregate group G). It is concluded as evident that FAMEs (Fatty Acid Methyl Esters) profile of cell can be used as a criterion in classification of lactic acid bacterial from kimchi. Additional comparative taxonomic studies need to be carried out on well chosen representative strains to determine the most appropriate methods of value.

비만 개선 효과를 지닌 탄수화물 및 지방 흡수 억제 기능성 식이조성물 개발

윤유식,최선미,홍순복,홍정미,김정원,이홍석,홍성길 한국조리과학회 2002 한국식품조리과학회지 Vol.18 No.3

In a previous study, we developed a new food additive as an egg yolk antibody (IgY) against carbohydrate digestion enzymes for the regulation of blood glucose level and weight control. The IgY delayed and decreased the increment of blood glucose level after administration of sucrose in human being by 30% in 20∼30 min. We also developed a lipase inhibitor as a water extract of two kinds of herb, Platycodon grandiflorum and Solanum melongena. Twenty three volunteers were subjected to the intake of the egg yolk IgY plus the herbal extracts for 50 days. In average, the treated subjects appeared to lose 1.96 ㎏ of body weight and 3.4 ㎏ of body fat mass during the treated period. Furthermore, Panniculus adiposus and breech size were significantly decreased during the experimental period. Above results suggested that the administration of the dietary additives composed of egg yolk IgY and natural herbal extract improve the obesity by the decrement of body weight and body fat mass.

야뇨증 환아들의 심리사회적 특성에 대한 다기관 연구 : 행동 및 정서 문제를 중심으로

조수철,김재원,신민섭,황준원,한상원,박관현,이상돈,김경도,김건석,서홍진,이유식,정재용,김영균,문두건,남궁미경,한창희,조원열,김영식,배기수,이종국,정우영,신의진 大韓神經精神醫學會 2005 신경정신의학 Vol.44 No.6

Objectives : The aim of this study was to examine the behavioral and emotional problems associated with nocturnal enuresis in Korean children. Methods : Three hundred eighteen children with nocturnal enuresis, together with their parents, completed the Child Behavior Checklist (CBCL), Disruptive Behavior Disorder Scale according to DSM-IV (DBDS), Children's Depression Inventory (CDI), State-Trait Anxiety Inventory for Children (STAIC), and Piers-Harris Children's Self-Concept Scale (PHCSC). Ninety-three normal students were selected as the control group. Results : Compared to the normal control group, the mean scores with regard to the withdrawn, social problems, attention problems, delinquent behavior, aggressive behavior, externalizing problems and total problems profiles were significantly higher in the nocturnal enuresis group according to the CBCL results. The nocturnal enuresis group also scored significantly higher in the ADHD and ODD profiles of the DBDS. The nocturnal enuresis group was more depressed and anxious than the control group according to the results of the CDI and STAI. The mean score of the PHCSC was significantly lower in the nocturnal enuresis group when compared to the normal control group. Conclusion : The results of this study suggest that children with nocturnal enuresis in Korea have clinically relevant behavioral and emotional problems. The findings support the link between nocturnal enuresis and psychopathology in Korean children.

객체 지향 데이타베이스 시스템의 질의 및 객체 관리기 인터페이스

이재봉,배석찬,이홍로,이연식 群山大學校 1993 論文集 Vol.20 No.-

객체 지향 데이타 모델을 지원하는 데이타베이스 시스템은 기존의 데이타베이스의 단점을 보완하며, 다양하고 복잡한 실세계의 정보를 처리할 수 있다. 객체 지향 데이타베이스 시스템은 사용자의 접근을 위해 질의 및 객체 관리 인터페이스를 필요로 한다. 본 논문에서는 사용자를 위한 질의 인터페이스와 질의 계획을 수행하기 위한 객체 관리 인터페이스를 설계함으로써 질의 목적을 달성토록 하였다. 설계된 객체 관리 인터페이스는 응용을 위해 "C"언어 함수로 구성하였고, 성능 항상을 위한 확장성을 제공할 수 있도록 설계 하였다. The database system that supports object oriented data model can complements conventional DBMS's drawbacks and processes complex and intensive applications. In object oriented database system, the access and management of all objects are manipulated by object manager. Therefore, a query interface as well as an Object Manager Interface(OMI) is required for interfacing between a user and an object store. In this paper, we propose the query interface which can support object oriented paradigm and design rules of OMI, and implement OMI based on the rules. The OMI have been implemented by using C language on UNIX OS machine.

敎師로서의 自覺이 精神病 經過에 미치는 影響

李詩炯,申泓湜,尹錫夏 大韓神經精神醫學會 1973 신경정신의학 Vol.12 No.3

This study is aimed at finding out the possible correlation on the influences of professional identity to the clinical symptomatology and progress among the schizophrenic patients. Eight of teacher patients, who were diagnosed as paranoid schizophrenia, over 40 years old, and have had more than 15 years of teacher carrier, were studied in the comparison with other schizophrenics with the same age range and dianosis. Result ; Teacher patients showed; 1) Regressive and aggressive behaviors were much lesser 2) Ward adjustment is better 3) Socialization and recover is faster 4) Precipitating event was identified in more cases In summary, teacher identity was well reflected on their symtomatology and progress, thus it had given a good outcome to their clinical course.

어항입구 부근에서 Pit식 방파제를 이용한 쇄파 발생억제 연구

이홍식,이보형 中央大學校 建設環境硏究所 1998 環境科學硏究 Vol.9 No.1

The effect of wave decay due to single pit breakwater has been studied for restraining from occurring breakers in the vicinity of a fishing harbor entrance. Firstly, by utilizing Green's theorem and boundary element approach, wave diffraction due to a pit breakwater is performed to investigate wave interaction with linear wave theory. Diffraction patterns in wave fields have been shown by the different various pit geometries. Secondly, by using an appropriate numerical model based upon the time-dependent analysis including wave refraction, diffraction, and partial reflection is adopted to study the effect of various pit geometries near to a harbor entrance. Application of various pit breakwaters for a fishing harbor been illustrated to present the effect of wave decay. Finally, in accordance with the condition of each pit breakwater, wave heights in the direction of leeward can be found reduced to 10~20% for the incident wave height.

항파상풍 톡소이드 주사후 항파상풍 항체 역가

이한식,하영록,구홍두,장석준,심호식,김승환,이정운 대한응급의학회 1996 대한응급의학회지 Vol.7 No.2

Objective : Tetanus decreased in prevalence since the systemic vaccination began in 1940s. Despite of the improvement in treatment like critical care with ventilatior or antibiotics, the mortality rate resides around 45%. Currently the tetanus prevention protocol recommends immunization in infancy and boosters every tenth years. Thereafter the immunization with toxoid only or toxoid plus immunoglobulin injections is recommended according to the type of injury. In most of ED in Korea, only tetanus immunoglobulin is given without any basis. Previously we proved the effect that passive immunization with the immunoglubulin 250 IU last long for only a month. At this time we measured the effect of the active immunization with toxoid and its effect was compared to the effect of the tetanus immunoglobulin to find out the logical tetanus prevention after an injury. Method: 20 healthy adult volunteers were injected with tetanus toxoid and their anti-tetanus antibody titers measured before the injection, 1 week and 4 weeks after the inection. No volunteers have taken any medication for chronic illness(e.g. hepatits, tuberculosis) or had an tetanus immunization or booster in the last 6 months. Antibody titers were measured by IMMUNOZYM??-TETANUS and t-test was performed on the results, Results: 1. Total 20 volunteers(12 males and 8 females) were participated. 2. Subjects` ages were 21 to 44 years old and there were no relevance to the antibody titer. 3. The antibody titers of before the anti-tetanus toxoid injection and 1 week, 4 weeks after the injection revealed significant difference and the antibody titers of the first and the forth week after the injection also showed a significant difference. Conclusion: Currently in Korea, the tetenus immunoglobulin 250 IU given alone as tetanus prevention was injected, but it did not elevate the anti-tetanus antibody titer for 4 weeks where as tetanus toxoid injected intramuscularly did significantly. Therefore, tetanus toxoid should be given on the dirty injuries with additional tetanus immunoglobulin injection to accomplish the correct method of tetanus, tetanus toxoid prevention.

호안전면의 이안제 배치조건에 따른 파랑-구조물의 상호작용

이홍식,김성덕,조원철 중앙대학교 건설환경연구소 2000 環境科學硏究 Vol.11 No.2

The structure-wave interactions have been studied to predict the wave fields at the coastal revetment around. The numerical modeling in the present study was based on the linear diffraction theory and boundary element approach. To investigate the interaction, the simulation has been carried out by varying the conditions of distance and length of an offshore breakwater in constant water depth. As result, various aspects of wave fields have been shown by geometric conditions of an offshore breakwater. It was found that the high waves have been significantly shown at coastal revetment around in cases of s/L=0.75 and 1.25.

소파식 케이슨 방파제에 작용하는 반사파의 추정

이홍식,남광훈 중앙대학교 건설환경연구소 2001 環境科學硏究 Vol.12 No.2

유수부를 갖는 소파식 케이슨 방파제에 대한 파랑의 거동을 예측하기 위하여 파랑과 구조물의 상호작용에 관하여 연구하였다. 케이슨 외부의 전면의 유공판은 얇은 판으로 구성하였다. 유수부에 의한 에너지 소산은 Damping function에 근거하여 모형화 하였고, 포텐셜 흐름과 선형파 이론을 사용하여 경계치문제를 공식화하여, 고유함수확장 기법에 의해 해를 구하였다. 단일 유수부를 갖는 소파식 케이슨에 대하여 반사계수 및 Damping계수를 기존의 실험결과와 비교하였다. 본 연구에서의 소파식 구조물에 대한 반사율의 이론적 예측을 기존의 연구결과와 비교한 결과, 유수부내에서 Damping효과의 합리적인 선택에 의하여 이론치 및 실험치와의 결과 차이를 크게 개선할 수 있는 것으로 나타났다. Analytical solution is presented to model the interaction of linear waves with absorbing-type caisson breakwaters, which possess one perforated front in one interior fluid regions (chambers). The perforated surface is idealized as thin porous plates. Energy dissipation in the interior fluid region inside the breakwater is modelled through a damping function. Under the assumption of potential flow and linear wave theory a boundary-value problem may then be formulated to describe wave interaction with the idealized structure. A solution to this simplified problem may be obtained by an eigenfunction expansion techique and an explicit analytical expression may be obtained for the reflected wave height. Using the experimental work of previous authors, damping coefficients are determined for single chamber absorbing-type caisson breakwaters. The theoretical predictions of the reflection coefficients for the one-chamber structures using the present model are compared with those obtained from laboratory experiments by other authors. It is found that the inclusion of the damping in the interior fluid region gives rise to improved agreement between theory and experiment.

생쥐 피부의 창상치유에 관한 형태학적 관찰

이향주,이영호,김홍선,김원식 충남대학교 의과대학 지역사회의학연구소 1989 충남의대잡지 Vol.16 No.1

The back skin of ICR mice were incised. The skin tissues were obtained from the incised wound on the 1st day, 1st weeks, and 2nd week after incision. And these tissue were fixed in 10% neutral buffered formalin and 2.5% glutaraldehyde solution. And, the formalin-fixed tissues were stained by PAS and alcian blue-safranin for the research of morphological changes of epidermis and the mast cells and granulation tissues of dermis. The tissues fixed in 2.5% glutaraldehyde solution were prepared for scanning electron micros-copy, and observed the crusts of scar surface. The results were as follows. 1. The collagen fibers in he granulation tissues at wound region were increased gradually, and became more dense than normal skin on the 2nd week after incision. 2. The newly-formed epidermis was thicker than that of normal skin at the 1st week, and increased in thickness to the 2nd week after incision gradually. 3. The mast cells were decreased in number than those of normal skin on the 1st day after incision, however, increased than those of normal skin on the 1st week after incision. On the 2nd week, the number of the mast cell were similar to those of normal skin. 4. Mixed mast cells containing both histamine and heparin appeared the granulation tissues on the 1st day and 1st week after incision. 5. In scanning electron microscopy, fibrin network on the scar began to appear on the 1st day, formed in a row on the 1st week. But fibrin network changed into irregular rough crusts on the 2nd week after incision.