김규동의 시세계 연구

김은영(Kim Eun-young) 국어국문학회 2010 국어국문학 Vol.- No.156

Kim, Gyu-Dong is a very unusual poet who has transformed into a dynamic process about his poetry. He was a very well known modernist when he took an active part of the 1950's modernism coterie 'Hubangee . However, in the 1970's, he changed himself as a socialized poet. I took care that realistic attitude he had shown in the 1950's and studied about it. I found the special point where the modernist Kim, Gyu-Dong had met the realist himself is shown in the connection between the poetry and the film. As everyone knows, Kim, Gyu-Dong prefer the automatism of surrealism as a method writing poem. And Kim, Gyu-Dong also had an inclination for the film Avant-Garde. Because the automatism of surrealism and the film Avant-Garde have the common theoretical base with the Freud's psychoanalysis. A lot of poems written by Kim. Gyu-Dong in the Korean war period show us special feature taking surrealism technique and the sense of the film Avant-Garde. He tried out express about the desperation and pain of the Korean people lived in that same period with his poems. But unfortunately, some several poems lost the critical mind and fell to nihilism. Kim, Gyu-Dong wanted to resist to the Korean social contradictions and conflicts continuedly. So he of course chose the more realistic writing method and concerned with the film Neo-Realism. He described some kind of the Nee-Realism character and circumstance in his poems for example 〈acrobat〉, 〈small hand〉. He described them as like using camera of the Neo-Realism film for presentation the problem of Korean 1950's society and community. And That's why he was very interested on the film Neo-Realism. So we can realize the true that his 1950's poetry had obvious inclination of realism already. It explains us about the connection between Kim, Gyu-Dong s 1950's poetry and 1970's poetry.

보건소의 노인정 운동프로그램 실시에 따른 노인의 인지지각요인, 건강증진행위 및 생활만족도 비교

김은영,전경자,Kim, Eun-Young,June, Kyung-Ja 한국지역사회간호학회 2000 지역사회간호학회지 Vol.11 No.1

This study was designed to investigate effects on cognitive perception. health promotion activity and life satisfaction of elderly. The participants for this study were 292 in senior citizen's center in S-city. The participants were categorized by two groups. one have taken exercise regularly. at least more than one a week for 6 months, the other have not. The measurement tools were the Perceived Benefit Scale developed by Walker, et al. and translated by Kim. Hee Ja(1994), the Life Satisfaction Index developed by Neugarten. etc. and translated by Lee. Ga Ok(1994) and Self efficacy Scale developed by Kim. Hee Ja (1994). The data were collected by personal interviews. which were taken from January 11 to 16. 1999, and analyzed by SAS 6.12. The major results of this study were as follows 1) Scores of self-efficacy were higher regular exercise group than irregular group. 2) Regular exercise group have taken exercise such as jogging, climbing and the other hands irregular group have not. 3) When the participants were categorized by smoking, other health promotion behavior (weight control. diet), both scores of life satisfaction index were higher smoking group than non-smoking group, other health promotion behavior than non-health promotion behavior. 4) There was no significant difference between the two groups in terms of perceived benefit, life satisfaction, From these findings, regular exercise program will be able to provide elderly with opportunity to change positively their lives in cognitive perception. health promotion activity.

<안개>(1967)와 모더니즘 영화 논의에 대한 비판적 검토

김은영(Eun-Young Kim) 한국여성커뮤니케이션학회 2017 미디어, 젠더 & 문화 Vol.32 No.2

영화 <안개>(1967)는 한국영화로서는 드물게 모더니즘 경향을 탑재한 영화로 손꼽힌다. <안개>는 김승옥의 「무진기행」(1964)을 원작으로 한 ‘문예영화’로서, 당시의 지식인 사회에서 인정받았을 뿐만 아니라 흥행에도 성공한 영화였다. 김승옥이 직접 각색에 참여했기 때문에 원작의 ‘모던함’도 비교적 잘 보존되었다. 소설의 감수성과 미학적 자의식도 무리 없이 이전되었다. 2000년대 이후 <안개>를 재조명한 연구들은 그 시각적 실험에도 주목하여 이 영화가 네오모던 시기의 영화작가들이 성취한 모더니즘 영화에 속한다고 주장해왔다. 그러나 <안개>는 근대적 주체가 경험한 일상을 통속적인 멜로드라마의 틀 안에 가두고 주체의 불안과 갈등을 남녀의 문제로 축소한 영화이기도 했다. 통속성은 모더니즘 소설이 내재하고 있던 새로움까지도 약화시켰다. 요컨대 <안개>는 1960년대 유럽의 예술영화를 동경한 한국 영화생산자들이 예술적 정당성을 인정받으려한 기획예술영화였지만, ‘문예’와 ‘모던’사이를 넘나들면서 관객의 기호와 타협한 대중영화이기도 했다. The Mist, which adapted Seung-Ok Kim’s A Trip to Mujin(1964), has been recognized as one of the most remarkable Korean Modernist films as well as literary films in Korea. It was an ambitious film that tried to obtain the legitimacy as an art. The story has contained the invisible anxiety of an unstable middle-class man who came to his hometown from Seoul. It also made some achievements in terms of the aspects for visualizing feelings of uneasiness and contradictory situations of the modern life. These Modernist elements have originated from A Trip to Mujin and the script written by Seung-Ok Kim himself. However, throughout the filmmaking the Mist has transformed into a typical popular melodrama that dealt with an short affair between man and woman. Therefore the Modernist characteristics has weaken and distorted. It was far from the cinematic heritage of the Neo-modern period in the West. As a matter of fact, Modernist filmmakers have always attempted to create their own new pieces that had never been produced before. In conclusion, even though The Mist had tried innovative visual experiments, it was a film that had strived for popular success rather than artistic achievement.

태음인 청폐사간탕이 lipase 활성저해 및 db/db mouse의 체중감량, 혈장지질, UCP 1, 2 발현에 미치는 영향

김은영,설유경,최재중,전수형,김훈,김종원,Kim, Eun-Young,Sul, Yu-Kyung,Choi, Jae-Jung,Jeon, Soo-Hyung,Kim, Hoon,Kim, Jong-Won 사상체질의학회 2007 사상체질의학회지 Vol.19 No.1

1. Objectvies This experimental study was designed to investigate the effect of cheongpesagan-tang extract on the obstruction of the lipase activity and weight, plasma, UCP1, 2 mRNA in db/db mouse. Material and Methods: The body weight loss, food intake, feeding efficiency ratio, weight of the internal organs (liver, kidney, epididymal fat, brown adipose tissue), plasma glucose, triglyceride, total cholesterol, white adipose tissue, adipocyte size distribution, expression of UCP1, 2 mRNA were measured in db/db mouse administered Cheongpesagan-tang extract for 6 weeks. These were then compared with those of control groups administered the diet. 2. Results 1) Inhibitory effect against lipase activity was Kilgyung(81.7%), Nabokja (73.1%), Seungma(73.0%), Daewhang (68.4%), Kalgeun (55.3%), Kobon(34.5%), Hwanggeum(4.2%). 2) In the sample group, the body weight was significantly decrease than that of control group. 3) In the sample group, the weight of epididymal fat showed significantly decrease than that of control group. 4) In the sample group, triglyceride showed significantly decrease than that of control group. 5) In the sample group, distribution of adipose tissue showed significantly larger than that of control group. 6) In the sample group, UCP1, 2 mRNA in BAT showed significantly increase than that of control group. 3. Conclusions These results show that cheongpesagan-tang has an effect on the treatment of obesity.

세포외 Protease를 생산하는 내염성 Bacillus sp. SJ-10 균주의 분리 동정 및 효소 특성

김은영,김동균,김유리,최선영,공인수,Kim, Eun-Young,Kim, Dong-Gyun,Kim, Yu-Ri,Choi, Sun-Young,Kong, In-Soo 한국미생물학회 2009 미생물학회지 Vol.45 No.2

오징어 젓갈로부터 호염성 균주를 분리하여 형태적, 생리 생화학적 특성 및 분자계통학적 분석을 통하여 동정하였고, 이 균주가 세포외로 생산하는 호염성 protease를 정제하여 그 특성을 분석하였다. 본 균주는 NaCl 0~14%, $20\sim55^{\circ}C$ 및 pH 5~8에서 성장하였으며, $35{\pm}5^{\circ}C$, pH 7 및 5% NaCl에서 최적으로 성장하였다. 주요 지방산 조성은 anteiso-$C_{15:0}$ (44.70%), anteiso-$C_{17:0}$ (15.76%) 및 $C_{16:0}$ (13.91%)이었고, menaquinone은 MK-7이었으며, DNA G+C 함량은 50.58 mol%였다. 16S rRNA 유전자 염기서열을 통한 계통분석 결과 Bacillus이었으며 Bacillus sp. SJ-10으로 명명하였다. SJ-10 균주 배양액으로부터 40% 황산암모늄 침전과 Mono Q 컬럼크로마토그래피 과정을 거쳐 collagen과 gelatin을 특이적으로 분해하는 약 40 kDa의 세포외 protease를 정제하였다. 이 효소는 $35{\pm}5^{\circ}C$, NaCl $5{\pm}1%$ 및 pH 8에서 최적 활성을 나타냈으며, pH 5~10 범위에서 안정하였다. A bacterium producing the halotolerant extracellular protease was isolated from squid jeotgal, and was identified as Bacillus sp. SJ-10 based on morphological, physiological and biochemical characteristics, as well as phylogenetic analysis using 16S rRNA gene sequence. The strain grew at $20^{\circ}C\sim55^{\circ}C$, pH 5~8, and 0%~14% NaCl and optimal growth conditions were $35{\pm}5^{\circ}C$, pH 7, and 5% NaCl. The major cellular fatty acids were anteiso-$C_{15:0}$, anteiso-$C_{17:0}$, and $C_{16:0}$ DNA G+C content was 50.58 mol% and menaquinone consisted of MK-7 Phylogenic analysis based on the 16S rRNA gene sequence indicated that SJ-10T belongs to the genus Bacillus. About 40 kDa of the salt-tolerant protease was purified by 40% ammonium sulfate saturation and Mono Q column chromatography. The optimal activity of the protease was pH 8 and stable at pH 5~10. The optimum temperature and NaCl concentration were $35{\pm}5^{\circ}C$ and $5{\pm}1%$, respectively.

동결-융해된 인간 배반포기 배 유래의 배아 간(幹) 세포 배양

김은영,남화경,이금실,박세영,박은미,윤지연,허영태,조현정,박세필,정길생,임진호,Kim, Eun-Young,Nam, Hwa-Kyung,Lee, Keum-Sil,Park, Sae-Young,Park, Eun-Mi,Yoon, Ji-Yeon,Heo, Young-Tae,Cho, Hyun-Jung,Park, Se-Pill,Chung, Kil-Saeng,Lim, Jin 대한생식의학회 2001 Clinical and Experimental Reproductive Medicine Vol.28 No.1

Objective: This study was to establish the human embryonic stem (ES) cells derived from frozen-thawed blastocyst stage embryo that were destined to be discarded after five years in routine human IVF-ET program. Methods: Frozen-thawed and survived human blastocysts were treated by immunosurgery, and recovered ICM cells were cultured onto STO feeder cell layer and ICM colony was subcultured by mechanical dissociation into clumps. To identify ES cell, alkaline phosphatase staining and expression of Oct4 in replated ICM colonies were examined. Also, to examine the possibility of ES cell differentiation, retinoic acid (RA), basic fibroblast growth factor (b-FGF), nerve growth factor (NGF) were added in culture medium. In addition, to classify the specific cell type, differentiated cells were stained by indirect immunocytochemistry. Results: One ICM colony recovered from frozen-thawed six blastocysts was subcultured, continuously replated during 40 passage culture duration without differentiation. Subcultured colonies were strong positively stained by alkaline phophatase. When the expression of Oct4 in cultured ES colony was examined, Oct4b type is more clearly indicated than Oct4a one although there was not detected in embryoid body or differentiated cells. In differentiated cardiomyocytes from ES colony, cells were beaten regularly (60 times/min). In differentiated neural cells from ES colony, neurofilament (NF) 200 kDa protein, microtubule associated protein (MAP) 2 and ${\beta}$-tubulin of specific marker in neurons, glial fibrillary acidic protein (GFAP) of specific marker in astrocytes and galactocelebrocide (GalC) of specific marker in oligodendrocytes were confirmed by indirect immunocytochemistry. Also, muscle cells were detected by indirect immunocytochemistry. In addition, ES colonies can be successfully cryopreserved. Conclusion: This study suggested that establishment of human ES cells can be successfully derived from frozen-thawed blastocysts that were destined to be discarded, and obtained specific cell types (cardiomyocytes, neurons and muscle cells) through the in vitro differentiation procedures of ES cells.

A New Efficient Cryopreservation of Human Embryonic Stem Cells by a Minimum Volume Cooling Method

김은영,박세영,윤지연,길광수,이창현,이건섭,태진철,김남형,이원돈,정길생,박세필,임진호,Kim, Eun-Young,Park, Sae-Young,Yoon, Ji-Yeon,Ghil, Gwang-Su,Lee, Chang-Hyun,Lee, Gun-Soup,Tae, Jin-Cheol,Kim, Nam-Hyung,Lee, Won-Don,Chung, Kil-Saeng,Pa The Korean Society for Reproductive Medicine 2004 Clinical and Experimental Reproductive Medicine Vol.31 No.1

연구 목적: 본 연구는 인간배아줄기세포 동결에 minimum volume cooling (MVC) 초자화 동결방법이 유용하게 이용될 수 있는지의 여부를 조사하고자 실시하였다. 연구재료 및 방법: 인간배아줄기세포 콜로니는 0.05% collagenase 처리와 기계적 처리에 의해 작은 clumps로 자른 다음 동결 방법에 따른 효율을 비교 검토하고자, i) 대략 40-50개의 clumps를 10% DMSO가 들어있는 동결액에 $5{\sim}10$분간 처리하여 1ml cryo-vial에 넣고 slow-cooling용 cryo-module에 장착하고 -80C에서 overnight한 후 $LN_2$에 침지하여 완만동결을 실시하거나 ii) 10% ethylene glycol (EG)이 들어 있는 동결액에서 5$\sim$10분 처리하고 30% EG과 0.5 mol sucrose가 들어 있는 동결액에서 30초간 처리하여 본 연구를 위해 개발된 MVC straw에 10 clumps씩 적재한 다음 $4{\sim}5$ MVC straw를 $LN_2$가 들어있는 cryo-vial에 넣어 MVC 초자화동결을 실시하였다. 융해 후 생존율을 조사하였고 배아줄기세포의 특성을 유지하고 있는지의 여부를 조사하였다. 결 과: 융해 후, 인간배아줄기세포의 생존율은 완만동결을 실시했던 군 (20.0%) 보다 MVC 초자화동결을 실시했던 군에서 (76.0%) 유의하게 높게 나타났다. 또한 회복과정에서 완만동결을 실시했던 군에서는 세포성장이 매우 더디고 안 좋은 반면, MVC 초자화동결을 실시했던 군은 배아줄기세포의 증식이 동결을 실시하지 않은 세포와 같은 상태로 2주 이후부터 빠르게 전환되고 회복되는 것을 확인할 수 있었다. 이와 더불어 MVC 초자화동결-융해 후 회복된 배아줄기세포에서 정상 핵형, alkaline phosphatase acitivity, SSEA-4와 TRA-1-60 염색 및 Oct-4 발현을 확인하였으며 체외분화의 특성도 확인하였다. 결 론: 새로이 개발된 MVC 초자화동결을 이용하면 인간배아줄기세포는 고유의 특성을 잃지 않고 성공적으로 동결될 수 있다.

대학생의 ADHD 경향이 우울에 미치는 영향: 대인관계 문제의 매개효과를 중심으로

김은영,김은주,Kim, Eun Young,Kim, Eun Joo 한국디지털정책학회 2021 디지털융복합연구 Vol.19 No.10

본 연구의 목적은 대학생의 ADHD 경향과 우울의 관계에서 대인관계 문제의 매개효과를 살펴보는 데 있다. 자료수집 기간은 2020년 10월부터 11월까지로 1개월 소요되었다. 대학생을 대상으로 ADHD 경향, 우울, 대인관계문제 척도로 이루어진 설문조사를 실시하여 자료를 수집하였다. 총 201부의 통계분석을 위해 SPSS와 AMOS 프로그램을 적용하여 구조방정식 분석을 실시하였다. 연구 결과, 대학생의 ADHD 경향, 우울, 대인관계 문제 변인 간에는 유의미한 정적 상관을 보였다. 그리고 대학생의 ADHD 경향이 우울에 미치는 영향에서 대인관계 문제는 완전 매개를 나타냈다. 이러한 연구 결과는 대학생의 ADHD 경향이 우울에 직접적인 영향을 미치는 것이 아니라 ADHD 경향이 대인관계 문제를 매개로 우울이 나타나는 것을 시사했다. 본 연구 결과를 통해 상담 및 임상 현장에서 대인관계 문제를 감소시키는 전략이 ADHD 경향을 가진 대학생의 우울의 예방 및 정신건강을 증진시키는데 도움이 될 것으로 사료된다. The purpose of this study is to examine the mediating effect of interpersonal problem on the relationship between ADHD tendency and depression in college students. Data collection started from October to November in 2020 and the duration of data collection was about 1 month. College students were administered questionnaires of ADHD tendency, depression, and interpersonal problem scales. Data from 201 college students were analyzed with structural equation model using SPSS and AMOS program. The results indicated that there were significant positive correlations among ADHD tendency, depression, and interpersonal problem in college students. And there was a full mediation effect of interpersonal problem on the relationship between ADHD tendency and depression in college students. The result suggests ADHD tendency do not directly influence on depression but ADHD tendency influence on depression through interpersonal problem in college students. Current findings can contribute to the counseling and clinical field by providing the strategy of reducing interpersonal problem to prevent the depression and enhance the psychological health of those who have ADHD tendency in college students.

마우스 림프구증식과 GM-CSF생성에 미치는 Berberine의 효과

김은영,노민희,정양숙,김형수,김광혁,Kim, Eun-Young,Rho, Min-Hee,Chung, Yang-Sook,Kim, Hyoung-Su,Kim, Kwang-Hyuk 한국생명과학회 2007 생명과학회지 Vol.17 No.5

본 연구에서는 berberine이 세포성 면역에 미치는 효과를 보기위하여 림프구증식과 GM-CSF생성을 보고자 하였다. 마우스 비장세포에 berberine을 작용시켰을 때 저농도에서 세포성장을 촉진함으로서 림프구증식에 자극제로서의 역할을 담당할 것으로 보인다. GM-CSF의 생성은 berberine 단독처리에 의해서 증가됨으로서 GM-CSF생성의 증가로 인한 세포성 면역반응의 상승이 예상되고 LPS나 Con A에 의한 GM-CSF의 생성을 저해함으로서 과도한 GM-CSF생성을 중간에서 차단할 것으로 보인다. 따라서 이러한 결과들은 앞으로의 추가적인 연구결과들이 있게 되면 임상에서의 면역요법과 항염증작용에 berberine이 이용될 가능성을 시사한다 하겠다. Berberine, an alkaloid initially isolated from chinese herbal medicine, has antibiotic activities against a variety of organisms including bacteria, viruses, fungi, protozoans, and chlamydia. Furthermore, berberine has shown a number of beneficial effects, including anti-tumor, anti-inflammation, and vasodilatory effects. In this work we have investigated the effects of berberine on lymphocyte proliferation and GM-CSF production in mice. Mouse splenocytes were incubated with berberine and concanavalin A(Con A) to observe the effects on cell proliferation. The culture supernatants of splenocytes exposed to berberine, berberine plus LPS, and berberine plus Con A were harvested to assay GM-CSF. The cell proliferation of nice splenocytes exposed to berberine only($1{\mu}g/ml$) was increased significantly more than PBS(control) group. But the Con A-induced cell growth was inhibited by berberine. The GM-CSF production from mice splenocyte culture exposed to berberine only was increased in comparison with PBS(control) group, but the production of it with LPS or Con A was inhibited by berbeline. The present findings may explain lympocyte proliferating and regulating effects of berberine.

내시경 겸자 생검으로 진단된 위점막 이형성증의 치료 방침

김은영,김진조,김병욱,박승만,Kim, Eun-Young,Kim, Jin-Jo,Kim, Byung-Wuk,Park, Seung-Man 대한위암학회 2010 대한위암학회지 Vol.10 No.1

목적: 위점막 이형성증은 "위선암과 연관이 있거나 침습성 위선암으로 발전할 가능성이있는 명백한 종양성 상피"로정의되고 있으며 만성위염에서의 암 발생기전에서도 전암성 병변으로 여겨지고 있다. 이에 저자들은 내시경 겸자 생검에서 위점막 이형성증으로 진단된 환자에 대한 적절 한 치료방침을 결정하고자 본 연구를 시행하였다. 대상 및 방법: 2006년 1월부터 2008년 12월까지 3년 동안 내시경 겸자 생검으로 위점막 이형성증으로 진단받고 내시경 점막절제 또는 내시경 점막하 박리술을 시행한 148예를 대상으로 하였으며 내시경 겸자 생검 결과와 최종 조직병리 결과를 비교하였다. 결과: 148예의 환자 중 고도 이형성증 63예, 저도 이형성증 85예였으며 최종 병리 결과 위선암 49예(33.8%), 고도이형성증 40예(27.0%), 저도 이형성증 59예(39.9%)였다. 고도 이형성증으로 진단된 63예는 최종 조직병리 결과 위선암34예(54.0%), 고도 이형성증 20예(31.7%), 저도 이형성증 9예(14.3%)였으며 저도 이형성증으로 진단된 85예는 위선암 15예(17.6%), 고도이형성증 20예(23.5%), 저도이형성증 50예(58.8%)였다.결론: 내시경 겸자 생검 결과 위점막 이형성증으로 진단된 경우 고도 이형성증의 경우는 내시경을 이용한 점막절제 또는 점막하 박리술을 통한 절제술을 시행하고 저도이형성증의 경우 주기적인 감시 및 재생검 뿐 아니라 정확한 진단을 위한 내시경 점막절제 등이 고려되어야 할것이다. Purpose: Gastric epithelial dysplasia (GED) was defined as "unequivocally neoplastic epithelium that may be associated with or give rise to invasive adenocarcinoma" and GED also represents a direct precursor of intestinal type adenocarcinoma of the stomach. The recommended treatment guidelines for GED in the medical literature are endoscopic mucosal resection (EMR) or surgery for high grade dysplasia (HGD) and annual endoscopic surveillance with biopsy for low grade dysplasia (LGD) The aim of this study was to determine the treatment plan for GED that is diagnosed by endoscopic biopsy. Materials and Methods: We enrolled 148 patients who were treated by endoscopic mucosal resection (EMR) or endoscopiccsubmucosal dissection (ESD) for GED: there were 63 patients with HGD and 85 patients with LGD and all of them were diagnosed by endoscopic biopsy from January 2006 to December 2008. The results of the final histopathologic reports after EMR or ESD were compared with the results of the endoscopic biopsies. Results: The final histopathologic results of the 148 patients with GED showed 49 (33.1%) patients with adenocarcinoma, 40 (27.0%) patients with HGD and 59 (39.9%) patients with LGD. Among the 63 patients with HGD, 34 (54.0%) patients had adenocarcinoma, 20 (31.7%) patients had HGD and 9 (14.3%) patients had LGD. For the 85 patients with LGD, 15 (17.6%) patients had adenocarcinoma, 20 (23.5%) patients had HGD and 50 (58.8%) patients had LGD Conclusion: Complete resection, including EMR or ESD, is needed for patients with GED diagnosed by endoscopic biopsy and they have HGD. For patients with LGD, EMR or ESD may be needed in addition to endoscopic surveillance with biopsy for making the correct diagnosis and proper treatment because of the possibility of adenocarcinoma.