자유곡면으로부터 단면정보를 이용한 황삭계획에 관한 연구

안대건,최홍태,이석희 한국경영과학회 1994 한국경영과학회 학술대회논문집 Vol.- No.1

This study deals with roughing planning by cross sectional information generated from sculptured surfaces. Bicubic Bezier surface is adopted as sculptured surfaces in this paper. The system consists of 3 parts : (1) modeling sculptured surface, (2) reconstruction of cross-section in 2D coordinates, (3) determination of roughing tool path with structural data. The system is developed by using IBM-PC in the environment of Auto CAD R11, AutoLISP and MetaWare High C. The proposed system shows an efficient algorithm for roughing planning with cross sectional informaiton.

그람양성구균에 대한 Teicoplanin과 Vancomycin의 시험관내 항균력

최태열,김경숙,전용관,서일혜,김정욱,이웅수,안정열,김홍석,정재용,최효선,김덕언,유진우 대한감염학회 1994 감염 Vol.26 No.1

An increasing frequency of methicillin resistant S. aureus(MRSA), methicillin resistant coagulase negative staphylococci(MRCNS) and Enterococcal infection have been observed in recent years. Teicoplanin is a new glycopeptide antibiotic obstained from the Actinoplanes teicomycetius. The molecular structure and spectrum of antimicrobial activity of teicoplanin is simillar to those of vancomycin, and has been reported to have an excellent in vitro and in vivo effect against various gram-positive infections. Therefore, we evaluated the in vitor susceptibility of gram positive cocci, such as, S. aureus, coagulase negative Staphylococci(CNS), and Enterococci to teicoplanin and vancomycin. The total 253 strains consisted of MSSA(40), MRSA(53), MSCNS(47), MRCNS(48), and Enterococci(65). They were assayed by disc diffusion and agar dilution. During the study, 57% of S. aureus and 49% of CNS showed resistance to methicillin. The inhibitory diameter of teicoplanin was 15-20mm in MSSA, 12-19mm in MRSA, 13-24mm in MSCNS, 11-23mm in MRCNS, and 15-22mm in Enterococci respectively, and showed sensitivity in all but 8 strains(3.2%). The range of the minimum inhibitory concentration (MIC) of teicoplanin to MSSA, MRSA, MSCNS, MRCNS and Enterococci were 9.12-2.0㎍/ml, 0.25-2.0㎍/ml, & 0.25-32㎍/ml, 0.12-1.0㎍/ml respectively. One case of S. haemolyticus was resistant to teicoplanin (32㎍/ml) by the agar dilution method. Eight minor (3.2%) and one major(0.4%) error was observed when the MIC and disk diffusion data were correlated with teicoplanin. As for vancomycin the inhibitory diameter was 17-21mm in MSSA, 15-21mm in MRSA, 18-26mm in MSCNS, 18-25mm in MRCNS, and 16-22mm in Enterococci respectively. The range of the MIC of vancomycin to MSSA, MRSA, MSCNS, MRCNS, and Enterococci were 0.25-1.0㎍/ml, 0.25-4.0㎍/ml, 0.5-2.0㎍/ml and 0.5-2.0㎍/ml respectively. One minor error (0.4%) was seen with the vancomycin disk. The MIC90 of MSSA and MRSA exhibited the same results in teicoplanin (1.0㎍/ml, 1.0㎍/ml), and vancomycin(2.0㎍/ml, 2.0㎍/ml). MSCNS and MRCNS exhibited greater MIC90 with teicoplanin(4.0㎍/ml, 8.0㎍/ml) than vancomycin(2.0㎍/ml, 2.0㎍/ml). Incontrase Enterococci were more susceptible to teicoplanin(0.5㎍/ml) than to vancomucin (2.0㎍/ml). Results from this analysis indicated that both teicoplanin and vancomycin were very excellent for gram positive infections, especially those resistant to methicillin.

서울 서남부지역 노인 인구에서 비만증 유병률과 고지혈증과의 상관성

최경묵,조영직,김경오,김동림,김난희,백세현,안정천,서홍석,오동주,최동섭 대한노인병학회 2000 Annals of geriatric medicine and research Vol.4 No.3

연령증가에 따른 생쥐 혈액-가슴샘 관문의 미세구조

안의태,최석봉,고정식,경홍기,한영복 대한해부학회 1994 Anatomy & Cell Biology Vol.27 No.4

종양치료제가 Ehrlich 암세포를 이식한 생쥐 가슴샘겉질에 미치는 영향 - 전자현미경적 연구

안의태,고정식,박경호,최석봉,한영복,경홍기,문정조 대한해부학회 1997 Anatomy & Cell Biology Vol.30 No.3

Lipopolysaccharide로 유발된 생쥐 무릎 관절염 완화에 미치는 진통산의 효과 : 윤활관절막과 섬유관절막의 형태 변화를 중심으로 Based on the Morphological Change of Synovial Membrane and Fibrous Membrane

김진택,최홍식,안상현,박인식,강윤호,김호현,이해풍 동국대학교 경주대학 1999 東國論集 Vol.18 No.-

Knee joint BALB/C mice were administered with JINTONGSAN(JTS) extract after Lipopolysaccaride(LPS) injection were observed to investigate the anti-inflammatiory effect of JTS for arthritis. The JTS extract(3.3 ㎖/㎏/day) were daily administered to mice suffered from arthritis of knee joint induced by LPS injection, as dose of 300㎖/㎏. The specimen were fixed in 4% paraformaldehyde and were decalcificate in EDTA solution for 4 Weeks. In synovial membrane, the hyperplasia of synovial lining cell(fibroblast like synoviocytes) on JTS group were diminished then LPS group. The number of synovial lining cell(synovial phagocytic cells) with filopodia and the infiltrated of lymphocyte on JTS group were decreased. In fibrous membrane, The region of fibrosis on JTS group were mitigated than LPS group and the appearance of fibroblast and migrated cell, as neutrophil leukocytes and lymphocyte, were decreased. As results indicated that JTS was effective in anti-inflammatory reaction for LPS-induced arthritis.

제대혈 분리방법의 차이에 따른 단핵구 및 조혈모세포의 수득 효과에 관한 비교연구

박정숙,이영호,최안홍,노신애,김태겸,한진영 대한조혈모세포이식학회 1998 대한조혈모세포이식학회지 Vol.3 No.1

연구배경 : 최근에 보고된 변형적 Ficoll-Hypaque 방법을 이용한 제대혈 분리방법이 지금까지의 고식적 Ficoll-Hypaque 분리방법에 비하여 조혈모세포의 수득효과면에서 얼마나 차이가 있는지 또한 비교적 수득효과가 크다고 알려져 있는 3% gelatin을 이용한 분리방법에 비하여 얼마나 효과적인지를 알아보고, 제대혈 분리방법의 차이에 따라 단핵구와 조혈모세포 사이에 어떤 상관관계가 있는지 알아보기 위하여 본 연구를 시행하였다. 방법: 21명의 건강한 산모로부터 정상 질식 및 제왕절개 분만 후 제대정맥에서 해파린 처리한 주사기를 사용하여 제대혈을 채취하였다 채취한 제대혈은 동일하게 3개월 시험관에 나누어 실온에서 24시간 이내에 고식적 Ficoll-Hypaque분리법, modified Ficoll-Hypaque 분리법, 3% gelatin 분리법을 이용하여 각각 단핵구 분리를 시도하였으며 이들의 단핵구수 및 생존률, CD34 양성세포수, 집락수를 측정 비교 하였다. 또 상관분석을 이용하여 단핵구수, CD34양성세포수와 CFU-GM집락수 사이의 상관관계를 알아보았다. 결과: 1) 세포 생존률은 고식적 Ficoll-Hypaque 분리법, 변형적 Ficoll-Hypaque 분리법, 3% gelatin 분리법 사이레 통계적 차이가 없었다. 2) 단핵구수는 3% gelatin으로 분리했을 때가 4.56±1.86 (×10^(6)/mL)로 수득효과가 가장 높았으며 (P=0.0001), Ficoll-Hypaque으로 분리했을때는 각각 1.54±0.06(×10^(6)/mL), 1.36±0.67(×10^(6)/mL)로서 고식적 방법과 변형적 방법 차이에 통계적 차이가 없었다. 3) CD34 양성세포수는 3%gelatin으로 분리했을 때가 6.08±3.99(×10⁴/mL)로 수득효과가 가장 높았으며 (P=0.0001), Ficoll-Hypaque으로 분리했을 때는 각각 2.18±2.17(×10⁴/mL), 2.24±1.63(×10⁴mL)로서 고식적 방법과 변형적 방법사이에 통계적 차이가 없었다. 4) CFU-GM 집락수는 3% gelatin으로 분리했을 때가 15.72±8.93(×10³/mL)로 수득효과가 가장 높았으며 (P=0.0002), Ficoll-Hypaque으로 분리했을 때는 각각 3.12±1.45(×10³mL), 2.35±0.82(×10³/mL)로서 고식적 방법과 변형적 방법 사이에 통계적 차이가 없었다, 5) 제대혈 고식적 Ficoll-Hypaque 방법으로 분리하였던 경우는 단핵구수와 CFU-GM집락수 사이에 상관관계가 없었으며CD34 양성세포수만 CFU-GM집락수와 상관관계가 있었다. (r=0.57). 또 변형적 Ficoll-Hypaque 방법에 의하여 분리하였던 경우는 단핵구수나 CD34 양성세포수 모두가 CFU-GM 집락수와 상관관계가 없었다. 그러나 제대혈을 3% gelatin법으로 분리하였던 경우는 CFU-GM집락수 가 단핵구수 (r=0.88) 및 CD34양성세포수(r=0.86)양자 모두와 상관관계가 있었다. 결론 : 3%gelatin을 이용하여 제대혈을 분리하는 것이 단핵구 및 조세모세포의 수득효과면에서 가장 효과적이었다. 한편 변형적 Ficoll-Hypaque 분리법이 고식적 Ficoll-Hypaque 분리법보다 시간과 경비면에서 비효율적이면서 수득효과도 높지 못하였다. 또 제대혈 분리법의 차이에 따라 단핵구나 CD34 양성세포와 VFU-GM사이에 상관관계에 차이가 있었으며, 실제로 제대혈이식 후에 생착 여부를 예측할 수 있는 인자가 제대혈 분리법의 차이에 따라 달라지는지에 관해서 보다 많은 임상적 연구가 필요할 것으로 사료된다. Background : Cord blood banking for stem cell transplantation requires volume reduction and red cell depletion for cost effective storage. To determine which technique could minimize the loss of progenitor cells during cord blood processing, we compared the efficacy of cord blood separating methods which were conventional Ficoll-Hypaque method, modified Ficoll-Hypaque method, and 3% gelatin method. Methods : Twenty-one cord blood samples were obtained with heparinized syringes from the umbilical vein following delivery. Three aliquots of each 20mL cord blood were stored at room temperature and processed by 3 different techniques within 24 hours after collection. We compared mononuclear cells(MNCs) counts and their viability, CD34+ cells counts, and CFU-GMs counts and also observed their correlations among three techniques. Results : 1) The yield of MNCs or CD34+ cells was highest in the cord blood processed with 3% gelatin method than conventional or modified Ficoll-Hypaque method(P=0.0001). 2) There were no significant difference in the yield of MNCs and CD34+ cells between conventional and modified Ficoll-Hypaque method. 3) Both MNCs counts (r=0.800) and CD34+ cells counts (r=0.86) were significantly correlated with CFU-GM counts in the cord blood processed with 3% gelatin method. 4) CD34+ cells counts were significantly correlated with CFU-GM counts in the cord blood processed with conventional Ficoll-Hypaque method (r=0.57), but MNC counts were not. 5) Both MNC counts and CD34+ cell counts were not correlated with CFU-GM counts in the cord blood processed with modified Ficoll-Hypaque method. Conclusion : Cord blood separation with 3% gelatin method could provide better yield of progenitor cells than with FICOLL_Hypaque methods. And between two Ficoll-Hypaque methods, modified method is more laborsome but not better in terms of clonogenic potential than conventional method.

대학생의 기본 심폐소생술 교육 평가 : 간호과와 비간호과를 대상으로

이정은,고봉연,이인모,최근명,박신일,안홍기 한국응급구조학회 2003 한국응급구조학회지 Vol.7 No.1

The purpose of this study was to evaluate the accuracy of CPR skills and the satisfaction of CPR Training targeted for college students. Also, this study made comparative study of nursing students and non-nursing students. The sample consisted of 248 students(132 nursing students, 116 non-nursing students). CPR Training was designed by two components which were a lecture and demonstration by one professor and individual practice 표 instructors. As tool of measurement estimation satisfaction of CPR Training questionnaires were developed based on educated contents. The accuracy of CPR skills were checked by SkillReporter CPR training manikin. Collected data were computerized and analyzed by SPSS-WIN program. The results were as follows : 1. The skills of CPR were cardiac compression 92.79 times/min, correct cardiac compression rate 90.85%, ventilation 9.93 times/min. correct ventilation rate 79.34%. 2. The mean of the accuracy of CPR skills were 2.94(SD .87). 3. When errors were analyzed, the highest item was stomach distension(51.2%) of ventilation skills and too little(70.6%) of cardiac compression skills. 4. The mean of the satisfaction of CPR training were 2.87(SD .17), the highest item of the satisfaction of CPR Training was practice. 5. We found significant statistical differences based on the accuracy and the error, non-nursing students were high in correct cardiac compression per minute(t=3.615, p=0...) and ventilation too much(t=4.292, p=.000), nursing students were high in correct ventilation rate(t=-3.885, p=.000) and cardiac compression too shallow) t=-2.842, p=.005).

기능성 생식, 다이어트식 및 장개선제가 비만개선에 미치는 영향

전태일,전은자,김창성,이주용,안홍석,임병우,최영숙,박웅채,박동기 建國大學校附設農業資源開發硏究所 2001 農資源開發論集 Vol.23 No.-

Obesity is defined as an excess of body fat and is closely related to diabetes, hypertension and heart disease. Dietary cure has been paid attention as a method for preventing from or curing obesity. The effect of some food products developed in this laboratory, such as, functional saengsik (Health & Joy saengsik), diet food (Health & Joy diet meal) and intestine function activator (Cell-pa, commercial name) on curing and protecting from obesity were investigated. Diet program was applied to 31 adults (Males 22, Females 9) with above 120% body mass index (BMI). After diet program was applied, body weight, body fat and fat weight decreased by 1, 4.8 and 4.5% (p<0.01), HDL-cholesterol increased by 3.6% and LDL-cholesterol and atherogenic index decreased by 6.6 and 7.7% (p<0.05). Consequently, the food products developed in this laboratory effectively reduced the obesity.

Induction of apoptosis by ethanol extract of <i>Citrus unshiu</i> Markovich peel in human bladder cancer T24 cells through ROS-mediated inactivation of the PI3K/Akt pathway

Ahn, Kyu Im,Choi, Eun Ok,Kwon, Da He,HwangBo, Hyun,Kim, Min Yeong,Kim, Hong Jae,Ji, Seon Yeong,Hong, Su-Hyun,Jeong, Jin-Woo,Park, Cheol,Kim, Nam Deuk,Kim, Wun Jae,Choi, Yung Hyun International Research and Cooperation Association 2017 Bioscience trends Vol.11 No.5