백서에서 동종 신경이식시 항 T임파구 단일클론항체 분포에 관한 조직화학적 연구

홍창섭,김경욱,임창준,정대현 단국대학교 치의학연구소 1991 논문집 Vol.3 No.-

Nerve allografts as a bridge through which lost nerve can regenerate is useful in the repair of peripheral nerve defect resulting from trauma, neoplasm and leprosy. But immunological rejection and complicated scar formation is an unaboidable problem in the application of allogenic nerves. This article is intended to study histopathological and immunohistochemical response after fresh allogenic sciatic nerve grafts in rats. 12 male Sprague- Dawley rats were used as the experimental animals. A 2cm skin incision was made on the lateral aspects of limb, parallel to the femur. 1cm-long sciatic nerve trunk taken from the one rat was resected at the middle of the to high, and it was transplanted to the same area of the other rat with perineural suture method. Observation was made at the postoperative 1, 3, 8, 16 day. The results were as follows. 1. Histopathologically, destruction of the perineural sheath was observed only in 1-day group. 2. Mild degenerative changes of the nucleus and cytoplasm of Schwann cells were ovserved in the 1-day and 3-day groups. These changes gradually resolved. 3. Hollow space phenomena of the nucleus and cytoplasm of schwann cells were observed. The response was mild in the 1 day and 3 day groups, and moderate in the 8 day group. The phenomena were not identified in the 16 day group. 4. Immunohistochemical studies revealed pan T cells and cytotoxic T cells in the 1 day group. Increased numbers of cytotoxic T Cells and helper T cells were observed in the 3 and 8 day groups. 5. According to these results, after transplantation of fresh allogenic sciatic nerve an inflammatory response was observed up to 8 days and then gradually resolved. Degenerative changes were more severe up to 8 days, but an the 16th day they had resolved histopathologically and immunopathologically.

평활근 증식과 점액성 기질 변화를 보인 사구맥관근종 1 예

김창덕(Chang Duk Kim),박재홍(Jae Hong Park),류영욱(Young Wook Ryoo),김병천(Byung Chun Kim),이규석(Kyu Suk Lee) 대한피부과학회 2001 대한피부과학회지 Vol.39 No.10

Glomus tumors are benign neoplasms that are derived from modified smooth muscle cells known as glomus cells. Histologically, it can be subdivided as glomus tumor proper, glomangioma, and glomangiomyoma according to relative proportions of components. Glomangiomyomas are the least frequent type and their overall architectural pattern may resemble glomus tumor proper or glomangioma, but there is a gradual transition from glomus cells to elongated mature smooth muscle cells. This transition is most obvious in the region surrounding large vessels. We present a case of glomangiomyoma of the left upper arm and the left fourth finger, in which ten-year history of two painful, bluish-colored subcutaneous nodules. On histologic examination, this case showed marked smooth muscle cell proliferation around large vessles and mucinous stromal change. (Korean J Dermatol 2001;39(10) 1147-1151)

전면문제중심학습이 치의학교육의 자기주도적 학습, 의사소통, 문제해결 능력에 미치는 영향

이강욱 ( Kang-wook Lee ),홍진실 ( Jin-sil Hong ),장기완 ( Kee-wan Chang ) 대한구강보건학회 2016 大韓口腔保健學會誌 Vol.40 No.4

Objectives: The purpose of this study was to examine the effect of problem-based learning (PBL) on dental education to dental students. The following were investigated in this study: 1. Does PBL improve the ability of self-directed learning? 2. Does PBL change communication ability? 3. Does PBL change the strategy of problem solving? Methods: The participants of this study were 39 students in the experimental group and 68 students in the control group. The measurement tools of this study were self-directed learning, communication, and problem solving abilities tests designed by the Korean Educational Development Institute. The data was analyzed by the two-way ANOVA and ANOVA with repeated measures. Results: In self-directed learning ability during 3 semesters, the experimental group showed a U-shape change but the control group showed a reverse U-shape change. In the experimental group, the selfdirected learning ability was decreased after one year (after the first and second semester). The level of communication ability decreased every day during the three semesters monitored in both the control and experimental groups. The level of communication ability in the experimental group continuously decreased during the three semesters, with the exception of one semester. Finally, for the problem solving ability during the three semesters, the experimental group showed a reverse U-shape change while the control group showed a U-shape change. In the experimental group, there was no change after two semesters. Conclusions: On the basis of the findings in this study, the following conclusions can be made. First, problem-based learning has a positive educational effect compared to didactic-based learning within one year. Second, the appropriate length of PBL is two semesters, or one year. It could be recommended that dental education in Korea should be combined or hybridized with PBL; for example, PBL could be used in combination with brief lectures or block lectures by teachers.

MODIS NDVI 시계열 패턴 변화를 이용한 산림식생변화 모니터링 방법론

정명희,이상훈,장은미,홍성욱,Jung, Myung-Hee,Lee, Sang-Hoon,Chang, Eun-Mi,Hong, Sung-Wook 한국공간정보학회 2012 한국공간정보학회지 Vol.20 No.4

정규식생지수(NDVI)는 식생자원을 모니터링할 수 있도록 설계된 식생지수(VI-Vegetation Index) 중 하나로 여러 응용 분야에서 가장 많이 사용되고 있는 지수이다. 산림 분야에서도 NDVI가 많이 활용되고 있는데 본 논문에서는 산림 변화 모니터링을 위해 MODIS NDVI를 활용하는 방법론이 연구되었다. 특정 시점을 기준으로 NDVI 값을 비교 및 분류하여 변화를 탐지하는 방법은 기계나 기상상태의 영향으로 자료의 정확성이 떨어질 수 있고 장기적인 변화를 탐지하는데도 어려움이 있다. 이러한 점을 고려하여 본 논문에서는 하모닉 모형을 이용하여 NDVI 시계열 자료를 통해 NDVI 패턴을 고려하는 방법론을 제시하였다. 먼저 하모닉 모형을 적용하여 미관측 자료나 자료의 오류를 보정한 NDVI 시계열 자료를 재구축하고 추정된 하모닉 요소의 모수를 기준으로 장기적 패턴을 통해 식생의 변화를 모니터링할 수 있다. 제안된 방법은 한반도 지역의 2009년 8월 21일부터 2011년 9월 6일까지 총 49개의 MODIS NDVI 시계열 자료에 적용하여 모형의 유용성을 입증하였다. Normalized Difference Vegetation Index (NDVI) has been used to measure and monitor plant growth, vegetation cover, and biomass from multispectral satellite data. It is also a valuable index in forest applications, providing forest resource information. In this research, an approach for monitoring forest change using MODIS NDVI time series data is explored. NDVI difference-based approaches for a specific point in time have possible accuracy problems and are lacking in monitoring long-term forest cover change. It means that a multi-time NDVI pattern change needs to be considered. In this study, an efficient methodology to consider long-term NDVI pattern is suggested using a harmonic model. The suggested method reconstructs MODIS NDVI time series data through application of the harmonic model, which corrects missing and erroneous data. Then NDVI pattern is analyzed based on estimated values of the harmonic model. The suggested method was applied to 49 NDVI time series data from Aug. 21, 2009 to Sep. 6, 2011 and its usefulness was shown through an experiment.

상피성 암세포주에 대한 녹차 Catechin의 효과

박정현(Jeong Hyun Park),김대중(Dae Joong Kim),한장희(Jang Hee Hahn),김홍태(Hong Tae Kim),정용욱(Yong Wook Jung),성훈기(Hoon Ki Sung),김주영(Joo Young Kim),송인환(In Hwan Song),성언기(Eon Gi Sung),이융창(Yung Chang Lee) 대한해부학회 2001 Anatomy & Cell Biology Vol.34 No.5

Catechin은 녹차에서 추출되는 폴리페놀의 주요성분으로 고혈압 및 동맥경화의 예방효과, 당뇨억제효과, 항산화작용, 항암작용 등에 직접 관여하는 물질이다. 본 연구에서는 태평양에서 제공받은 녹차 catechin을 이용하여 상피성 암세포주인 A549 (폐암)와 EATC (복수암)세포에 투여한 후 처리농도와 시간에 따른 변화를 비교, 관찰함으로써 녹차의 효과와 작용기전을 밝혀내는데 목적이 있었다. 본실험은 A549 세포와 EATC 세포를 배양한 후 녹차 catechin을 1, 10, 100, 500 μg/ml의 농도로 48시간 동안 처리하였고, 광학현미경, 공초점현미경, 전자현미경 등을 이용하여 세포의 구조적 변화를 확인하였으며 MTT분석, 전기영동, 유세포분석기 등을 사용하여 세포 손상정도를 파악하였다. A549 세포에서는 catechin 1 μg/ml와 10 μg/ml 농도에서는 대조군에 비하여 큰 변화를 관찰할 수 없었다. 100 μg/ml catechin을 처리하였을 때 세포내의 검은 과립들의 수가 증가하였고 층판소체의 손상이 나타났다. 세포주기의 장애가 나타나 DNA 합성전기에 있는 세포들의 수가 급격히 증가하였다. 500 μg/ml 농도에서는 층판소체와 사립체의 파괴가 심하게 나타났으며 세포생존율이 감소하였고 세포주기의 장애도 관찰되었다. EATC 세포에서는 catechin의 농도가 A549 세포의 경우보다 낮은 농도에서도 세포증식 억제 및 세포손상 효과가 나타났다. 10 μg/ml 농도에서도 세포의 위축과 생존율의 감소가 일어났으며 전기영동상에 괴사되는 세포들이 파악되었다. 100μg/ml catechin을 처리하였을 때 자연사의 형태학적 관찰, 전기영동, 유세포분석 등에서 자연사 과정에 있는 세포들이 많이 나타났다. 결과적으로 녹차 catechin을 배양한 상피성 암세포에 투여함으로서 세포의 생존율과 증식이 억제되었고 그 과정에서 괴사, 자연사, 세포주기의 장애 등이 관여하는 것으로 생각된다. 그러나 일련의 세포손상과정을 유도하는 데 있어 세포의 종류, 처리 시간, 농도에 따라 다소 차이가 있음을 확인하였다. Catechin is main component of polyphenol extracts from green tea, it is associated with prevention of hypertension and atherosclerosis, anti-diabetic effect, antioxidant, antitumor. The purpose of this research is to investigate the effect and its mechanism of green tea catechin on epithelial cancer cell lines in various concentrations and durations. For this study, epithelial cancer cell lines, A549 (lung cancer), EATC (Ehrlich-Lettre ascites tumor cell) were used. Inverted, light, confocal and electron microscopes were applied to find morphological changes. MTT assay, flowcytometric analysis, gel electrophoresis were used to compare severity of cellular damages to control after exposure to 1, 10, 100 and 500 μg/ml catechin for 48 hours. In the A549 cells, after 1 μg/ml and 10 μg/ml catechin treatments, there was no notable changes. However, exposure to 100 μg/ml catechin induced increase of cytoplasmic granules, destruction of lamellar body, inhibition of cell cycle, especially G0/G1. In the early phase of 500 μg/ml catechin administration, decrease of cell population, severe destruction of lamellar bodies and mitochondria, derangement of cell cycle were shown. In the EATC, such as those effects occurred after exposure to lower concentration of catechin than in that of A549 cells. After exposure of 10 μg/ml catechin, rounded-up cells and necrotic cells were found. Whereas, most of cells were under apoptotic changes-cytoplasmic condensation, nuclear fragmentation, cellular shrinkage, ladder pattern in the electrophoresis, when administrated 100 μg/ml catechin. These results suggested that exposure of catechin induced severe cellular damage and growth inhibition in dose- and time-dependent manner. And we confirmed that these effects of catechin were involved with apoptosis, necrosis and cell cycle arrest and were quite different according to cancer type. Therefore, much more research would be demanded before clinical application of catechin to human cancer therapy and this study would be the basic source for further study of green tea.

정신분열병에 대한 리스페리돈의 효과 및 안정성

이민수,김용구,김영훈,연병길,오병훈,윤도준,윤진상,이철,정희연,강병조,김광수,김동언,김명정,김상훈,김희철,나철,노승호,민경준,박기창,박두병,백기청,백인호,손봉기,손진욱,양병환,양창국,우행원,이정호,이종범,이홍식,임기영,전태연,정영조,정영철,정인과,정인원,지익성,채정호,한상익,한선호,한진희,서광윤 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.1

연구목적 : 본 시험의 목적은 임상시험 시작전에 연구자들을 대상으로 PANSS Workshop을 통하여 PANSS, ESRS에 대한 국내에서의 표준화 작업을 구축하고 새로운 정신병 치료제인 리스페리돈의 효과와 안정성을 재확인하여 리스페리돈 사용에 대한 적정화를 이루는데 있다. 연구방법 : 1996년 4월부터 1996년 9월까지 국내 39개 대학병원 정신과에 입원중인 혹은 증상이 악화되어 입원하는 정신분열병 환자 377명을 대상으로 다시설 개방 연구를 시행하였다. 1주일간의 약물 배설기간을 가진후, 리스페리돈을 8주간 투여하였고, 기준점, 1주, 2주, 4주, 그리고 8주후에 평가되었다. 용량은 제1일에는 리스페리돈 1mg씩 1일 2회, 제2일에는 2mg씩 1일 2회, 제3∼7일에는 3mg씩 1일 2회 투여하였다. 이후 환자의 임상상태에 따라 임의로 증량할 수 있으며, 최대 일일 16mg을 초과하지 않도록 하였다. 추체외로 증상을 조절하기 위한 투약을 허용하였다. 임상증상 및 부작용의 평가는 PANSS(Positive and Negative Syndrome Scale), CGI(Clinical Global Impression) 그리고 ESRS(Extrapyramidal Symptom Rating Scale)을 사용하였다. 연구결과 : 377명중 343명(91%)이 8주간의 연구를 완결하였다. 치료 종결시점인 8주후 PANSS 총점수가 20% 이상 호전된 경우를 약물 반응군으로 정의할때, 약물반응군은 81.3%였다. 리스페리돈에 반응하는 예측인자로는 발병연령, 이전의 입원 횟수, 유병기간이 관련 있었다. 리스페리돈은 1주후부터 PANSS양성, 음성, 및 일반정신병리 점수상에 유의한 호전을 보여 효과가 빨랐다. CGI의 경우도 기준점에 비해 1주후부터 유의한 감소를 나타내었다. ESRS의 경우, 파킨슨 평가점수는 기준점과 비교해 투여 1주, 2주, 4주후 유의하게 증가되었다가 8주후 기준점과 차이가 없었다. Dystonia 평가점수는 1주후만 유의한 증가를 보였으며, dyskinesia 평가점수는 유의한 차이가 없었다. 혈압, 맥박수의 생명징후 및 일반 혈액학 검사, 생화학적 검사, 심전도 검사에서 유의한 변화는 없었다. 결 론 : 이상의 다시설 개방 임상 연구를 통해 리스페리돈은 정신분열병 환자에서 양성증상뿐만 아니라 음성증상 및 전반적인 증상에도 효과적인 것으로 사료된다. 보다 명확한 평가를 위해서는 다른 항정신병약물과의 이중맹검 연구가 필요할 것으로 생각되며, 또한 장기적 치료에 대한 평가도 함께 이루어져야 하겠다. Objective : The purpose of this study was to investigate the efficacy and safety of risperidone in the treatment of Korean schizophrenic patients. Method : This multicenter open study included 377 schizophrenic patients drawn from 39 university hospitals. After a wash-out period of 1 week, the schizophrenic patients were treated with risperidone for 8 weeks and evaluated at 5 points ; at baseline, and 1, 2, 4 and 8 weeks of treatment. The dose was increased from 2mg/day(1mg twice daily) to 6mg/day(3mg twice daily) during the first week and adjusted to a maximum of 16mg/day over the next 7 weeks according to the patient's clinical response. Medication to control extrapyramidal symptoms was permitted. The psychiatric and neurological status of the patients was assessed by PANSS, CGI, and ESRS scales. Results : 343(91%) of 377 patients completed the 8-week trial period. Clinical improvement, as defined by a 20% or more reduction in total PANSS score at end point, was shown by 81.3% of patients. The predictors of response to risperidone were associated older age, shorter duration of illness, fewer previous hospitalization. Risperidone had rapid onset of action ; a significant decrease of the total PANSS and three PANSS factor(positive, negative, general), and CGI was already noticed at the end of first week. For the ESRS, parkinsonism rating scores were significantly increased until week 4 comparing with baseline. Dystonia rating scores were significantly increased until week 1, and dyskinesia rating scores were not significantly changed during the study. Laboratory parameters including vital sign, EKG, hematological, and biochemical values showed no significant changes during the trial. Conclusions : This study suggests that risperidone is generally safe and effective against both the positive and negative symptoms in our group of patients.

주파수 응답 함수를 이용한 평면 밀링에서의 채터 예측

홍성욱,박중윤,김현수,박재현,박창용 金烏工科大學校 産業技術開發硏究院 1999 産業技術開發硏究 Vol.15 No.-

The present paper investigates the chatter stability of a milling spindle-workpiece system in face milling process. To this end, a new method using frequendy response function is adopted for predicting the chatter vibration. A compliant workpiece is used to make the spindle-workpiece system easily exposed to chatter vibration. The entire experimental procedure, including measurement of frequency response function and cutting coefficients, is demonstrated. The experimental result shows that the chatter stability prediction method using frequendy response function is of great use in face milling process.

감염 조절용 차단막의 두께가 광중합기의 중합광에 미치는 영향

장훈상,이석련,홍성옥,류현욱,송창규,민경산 大韓齒科保存學會 2010 Restorative Dentistry & Endodontics Vol.35 No.5

연구목적: 본 연구는 감염 조절용 차단막을 여러 겹으로 사용했을 때 광중합기의 광강도와 파장, light diffusion 등에 미치는 영향에 대해 조사하였다. 연구 재료 및 방법: 감염 조절용 차단막은 투명 랩 (크린랩)을 사용하였고 광중합기는 할로겐 광중합기 (Optilux 360)와 LED 광중합기 (Elipar FreeLight 2)를 사용하였다. 차단막을 1겹, 2겹, 4겹, 8겹으로 광중합기의 광섬유말단을 감싸고 휴대용 광강도 측정기 (Cure Rite)로 광중합기의 광강도를 측정하였다. 광중합기를 주문제작한 optical breadboard에 고정시킨 후 휴대용 spectroradiometer (CS-1000)를 이용하여 광중합기의 파장을 측정하였고, DSLR (Nikon D70s)을 이용하여 광중합기의 light diffusion을 사진 촬영하였다. 결과: 광강도 측정 결과는 차단막의 두께가 증가할수록 광강도가 유의하게 감소하였으나 할로겐 광중합기에서 1겹과 2겹 사이에는 유의차가 없었으며, 4겹 이상의 차단막을 투과할 때 광강도가 더 많이 감소하였다. 여러 겹의 차단막을 투과한 광중합기의 전체적인 파장 형태와 peak wavelength의 변화는 관찰되지 않았다. Light diffusion 사진 촬영 시, LED 광중합기에서는 차단막의 두께가 미치는 영향이 없었으나 할로겐 광중합기에서는 차단막을 4겹 사용했을 때부터 중합광이 조사되는 각도가 감소하기 시작하여 8겹 사용했을 때 통계적으로 유의하게 감소하는 것을 볼 수 있었다 (p < 0.05). 결론: 광중합형 복합레진을 광중합할 경우 감염 조절용 차단막이 찢어지는 경우를 대비하여 1겹으로 사용하기 보다는 2겹으로 사용하는 것이 환자간의 교차감염을 예방하는데 유리할 것으로 사료된다. Objectives: This study investigated the effect of infection control barrier thickness on power density, wavelength, and light diffusion of light curing units. Materials and Methods: Infection control barrier (Cleanwrap) in one-fold, two-fold, four-fold, and eightfold, and a halogen light curing unit (Optilux 360) and a light emitting diode (LED) light curing unit (Elipar FreeLight 2) were used in this study. Power density of light curing units with infection control barriers covering the fiberoptic bundle was measured with a hand held dental radiometer (Cure Rite). Wavelength of light curing units fixed on a custom made optical breadboard was measured with a portable spectroradiometer (CS-1000). Light diffusion of light curing units was photographed with DSLR (Nikon D70s) as above. Results: Power density decreased significantly as the layer thickness of the infection control barrier increased, except the one-fold and two-fold in halogen light curing unit. Especially, when the barrier was four-fold and more in the halogen light curing unit, the decrease of power density was more prominent. The wavelength of light curing units was not affected by the barriers and almost no change was detected in the peak wavelength. Light diffusion of LED light curing unit was not affected by barriers, however, halogen light curing unit showed decrease in light diffusion angle when the barrier was four-fold and statistically different decrease when the barrier was eight-fold (p < 0.05). Conclusions: It could be assumed that the infection control barriers should be used as two-fold rather than one-fold to prevent tearing of the barriers and subsequent cross contamination between the patients.

전자상거래 사이트 구축 : 강원우드산업의 사례

이창수,홍민욱,임정희 강릉대학교 동해안지역연구소 2000 東海岸硏究 Vol.11 No.2

The purpose of this case is to construct the electronic commerce site of Kangwon Wood Industry Inc. in Sockcho City at Kangwondo. The major products of Kangwon Wood Industry Inc. are furniture, for example, bed, desk, table, and so on. The company want to enlarge its business opportunity to e-business, so it needs to establish the shopping mall. The shopping mall site has the menu of membership, order, view the products, cart, dispatching, administration mode, classification of products, registration of products, order list for administrator, bulletin board. One can see this site on http://tudori.kangnung.ac.kr/source/.

서울의 Penicillinase Producing Neisseria gonorrhoeae 발생빈도(1998)

김재홍,김준호,반재용,이정우,황성주,정준규,정성태,강진문,조흔정,홍창의,정혜신,이한승,김이선,이봉길,이종호,선영우,한기덕,윤성필,이성훈,안종성,박석범,문승현,조항래,김형섭,류지호,황재영,박준홍,손상욱 한양대학교 의과대학 2001 한양의대 학술지 Vol.21 No.1

In recent years, gonorrhea has been pandemic and remains one of the most common STDs in the world, especially in developing countries. For the detection of a more effective therapeutic regimen and assessing the prevalence of Penicillinase Producing Neisseria gonorrhoeae(PPNG), we have been trying to study the patients who have visited the Venereal Disease Clinic of Choong-Ku Public Health Center in Seoul since 1980 by menas of the chromogenic cephalosporin method. In 1998, 93 strians of N. genorrhoeae were isolated, among which 60(64.5%) were PPNG. The prevalence of PPNG in Seoul, which had been decreased to 39% in 1996 after a peak of 74.3% in 1993, is increased to 64.5% in 1998.