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한국산 생약으로부터 항암물질의 개발(제4보) 소엽 부탄올 가용분획의 항암활성
최규은,곽정숙,김영옥,백승화,한두석 한국독성학회 1997 Toxicological Research Vol.13 No.4
This study was carried out to develop antitumor effect of the n-butanol soluble fraction of Perilla frutescens on (KB cells) human oral epitheloid carcinoma cells. The cytotoxictty of methanollc extract of Perilla frutescens on KB cells was evaluated by 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide(MTT) assay. The antitumor activity of various fractions obtained from n-butanol soluble fraction of Perilla frutescens was evaluated in human oral epithelold carcinoma cells. The antitumor acavity of the n-butanol soluble fraction on human oral epitheloid carcinoma cells was evaluated by MTT assay of colorimetric method. The light microscopic study was carried out to observe morphological changes of cultured human oral epitheloid carcinoma cells. These results were obtained as follows; 1. The fractions 1,2 and 3 of the n-butanol soluble fraction of Perilla frutescens were shown significant antitumor activities. 2. The number of human oral epitheloid carcinoma cells were decreased and tend to form cell cluster by treatment with fractions 1,2,3 and 4 of the n-butanol soluble fraction of Perilla frutescens. 3. The fraction 1 of the n-butanol soluble fraction of Perllla frutescens showed the highest antitumor activity on Perilla frutescens. It has been selected as a lead fraction for further examinations.
한국산 생약으로 부터 항암물질의 개발 (제7보), 소엽의 Chloroform 가용성 분획이 인체 구강유상피암종세포에 미치는 세포독성작용
한두석,김영일,최규은,곽정숙,백승화,Han, Du-Seok,Kim, Young-Il,Choi, Kyw-Eun,Kwag, Jung-Suk,Baek, Seung-Hwa 한국환경성돌연변이발암원학회 1998 한국환경성돌연변이·발암원학회지 Vol.18 No.1
In the present study, we have evaluated cytotoxic effects of the chloroform soluble fraction of the methanolic extract of Perilla frutescens in human oral epitheloid carcinoma cells. The light microscopic study showed morphological changes of the treated cells. Cell membrane damaging activity was measured by the lactate dehydrogenase (LDH) assay and disruptions in cell organelles were determined by 3-(4,5-dime-thylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT), neutral red (NR) and sulforhodamine protein B (SRB) of colorimetric assay. These results suggest that Perilla frutescens retains a potential antitumor activity.
한국산 생약으로부터 항암물질의 개발(제6보). -금은화 Ethyl Acetate 가용성 분획의 인체 구강유상피암종세포에 미치는 세포독성작용-
한두석,백경현,김영옥,최규은,곽정숙,백승화,Han, Du-Seok,Baek, Kyong-Hyun,Kim, Young-Ok,Choi, Kyu-Eun,Kwag, Jung-Suk,Baek, Seung-Hwa 한국생약학회 1998 생약학회지 Vol.29 No.1
This study was carried out to develop antitumor agents based on effects of the ethyl acetate soluble fraction of the methanolic extract of Lonicerae flos on human oral epitheloid carcinoma cells. Human oral epitheloid carcinoma cells were cultured in RPMI-1640 media containing 10% fetal bovine serum, antibiotic, and fungizone. After incubation for 24 hrs, the cells were treated with A, B, C, D, and E fractions for 48hrs under the same condition. MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazoliumbromide), NR (Neutral red) and SRB (Sulforhodamine B protein) assay were performed. The light microscopic findings were observed by inverted microscope. In MTT assay, fraction B was shown significant antitumor activity (P<0.001), fraction E was shown significant antitumor activities (P<0.05), but the other fractions were not shown. In NR assay, fraction B was shown significant antitumor activity (P<0.001). In SRB assay, fractions B was shown significant antitumor activities (P<0.01). fractions A and D were shown significant antitumor activities (P<0.05). but the other fractions were not shown. In light microscopy. the fraction B of the ethyl acetate soluble fraction of the methanolic extract of Lonicerae flos showed the highest antitumor activity. These finding suggested that fraction B possessed the most antitumorous agent.
S-53 위 주위 농양을 모방한 외성장 위장관 간질종양
김동률,안지영,김형근,최규은,임은선,유지연,오정환 대한내과학회 2016 대한내과학회 추계학술대회 Vol.2016 No.1
서론: 위장관 간질종양의 임상양상은 복부 종괴(5~50%), 폐색(5%), 출혈 등 다양하다. 낭성 변화는 크기가 큰 경우 출혈 혹은 괴사에 이어 나타날 수 있고 농양 형성 및 천공은(0.8%) 드물다. 내시경 및 영상학적 검사에서 위 궤양 천공으로 인한 농양 형성 소견을 보였으나 수술 후 조직검사에서 위장관 간질종양이 확인되어 위 간질종양의 누공 형성이 위 주위 농양을 모방한 예를 보고한다. 증례: 77세 남자가 10여일 전 발생한 발열과 전신쇠약감으로 왔다. WBC 26,600/mm3, CRP 14.51 mg/dl 및 좌상복부 압통 있어 복부 컴퓨터촬영을 하였다. 좌상복부에 공기액체층을 보이는 9 cm 크기의 공동성 병변이 위와 연접해 있었으며 위 벽의 국소적인 붕괴가 의심되었다. 상부위장관 내시경상 위 대만곡 상체부에서 0.8 cm 크기의 동그란 구멍과 노란색 삼출물이 보였다. 구멍의 주변 경계부는 깨끗하여 악성을 시사하지 않았다. 위 내강과 농양의 교통이 확인됨에 따라 위 궤양에 의한 위 주위의 농양으로 생각하여 수술을 진행했다. 관련 부위를 쐐기 절제술로 제거하였다. H&E 염색에서 세포 밀도가 높은 spindle 모양의 세포가 관찰됐다. 면역화학염색검사에서 S-100 음성, actin 음성, CD117 양성 이었다. 최종진단은 위장관 간질종양으로 크기는 5~10 cm으로 추정되며 mitosis는 4/50 HPF 이었다. 수술 시 농양 주위 염증으로 Piecemeal resection하여 절제면 침윤은 알 수 없었다. 수술 후 15일째 imatinib 400 mg 복용을 시작하였으며, 이후 부작용 없이 외래 경과관찰 중이다. 고찰: 영상학적 검사에서 좌상복부 공기액체층을 보여 농양이 의심되며 내시경에서 누공이 확인되어 위궤양 천공으로 인한 위 주위 농양을 의심했으나 수술 후 조직검사에서 위장관 간질종양으로 확인되어 위 주위 농양을 모방한 위에 발생한 외성장의 위장관 간질종양 1예를 보고한다.
한국산 생약으로부터 항암물질의 개발 (제 5 보) - 소엽의 부탄올 가용분획이 인체피부흑색종 세포에 미치는 세포독성작용 -
이기남,신혁호,한두석,김영옥,최규은,곽정숙,백승화,Lee, Ki-Nam,Shin, Heuk-Ho,Han, Du-Seok,Kim, Young-Ok,Choi, Kyw-Eun,Kwag, Jung-Suk,Baek, Seung-Hwa 한국생약학회 1997 생약학회지 Vol.28 No.4
This Study was carried out develop antitumor effect of the n-butanol soluble of fraction of Perilla frutescens on human skin melanoma cells. The antitumor activity of various fractions obtained form n-butanol soluble fraction of Perilla frutescens was evaluated in human skin melanoma cells. The antitumor activity of the n-butanol soluble fraction in human skin melanoma cells was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay, neutral red (NR) assay and sulforhordamine B protein (SRB) assay of colorimetic assay methods. The light microscopic study was carried out to observe morphological changes of cultured human skin melanoma cells. These results were obtained follows; The fractions 5 and 6 of the n-butanol soluble fraction of P frutescens were shown significant antitumor activities. The number of human skin melanoma cells were decreased and tend to form cell cluster by treatment with actions 5 and 7 of the n-butanol soluble fraction of P. frutescens. The fraction 6 of the the n-butanol soluble fraction showed the highest antitumor activity on P. frutescens. These results suggest that the fraction 6 of the n-butanol soluble fraction of P. frutescens may be a valuable choice for the studies on the treatment of human skin tumors.
증례 : 내분비-대사 ; 제1형 신경섬유종증 가족에서 발견된 NF1 유전자 변이와 임상양상
정연정 ( Yeon Jeong Jeong ),서유림 ( Yoo Rim Seo ),최규은 ( Kyu Eun Choi ),한유민 ( Yu Min Han ),김은숙 ( Eun Sook Kim ),문성대 ( Sung Dae Moon ),한제호 ( Je Ho Han ) 대한내과학회 2016 대한내과학회지 Vol.90 No.5
With an incidence of 1 per 2,500-3,000 individuals, neurofibromatosis type 1 (NF1) is the most common autosomal dominant disorder in humans. NF1 is caused by germline mutations of the NF1 gene, but to date genotype-phenotype analyses have indicated no clear relationship between specific gene mutations and the clinical features of this disease, even among family members with the same mutation. The present study describes a case of two siblings with NF1 with the same genetic mutation but different clinical manifestations. The first patient was a female with iris Lisch nodules, an adrenal incidentaloma, Graves’ disease, and skin manifestations, while the second patient, the first patient’s younger brother, exhibited only skin neurofibromas and freckling. Further study is needed to reveal the molecular processes underlying gene expression and phenotypes. A better understanding of the genetics associated with NF1 will allow clinicians to detect complications earlier and provide better genetic counseling to NF1 families. (Korean J Med 2016;90:455-459)