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韓斗錫,朴承澤,崔玟圭,金丁中,鄭然泰 圓光大學校 醫科學硏究所 1986 圓光醫科學 Vol.1 No.2
Fine structure of perisinusoidal cells of the rat liver were examined by electron microscopy. Four different, cell types of perisinusoidal cells were observed. They are endothelial cell, pit cell, Kupffer cell, ana stellate cell. The cytoplasm of endothelial cells in sinusoids showed numerous fene-strations of different size, and nucleus was various with cell shape. Pit cells showed rod-cored vesicles and numerous electron dense granules, well developed pseudopodia, and nucleus almostly indented. Kupffer cells revealed numerous microvillous projections, and its nucleus was oval in shape. Perisinusoidal stellate cells were observed between endothelial cells and hepatocytes, and their cytoplasm contained numerous lipid droplets, some glycogen granules, and numerous mitochondria and its nucleus was oval in shape.
페놀 화합물의 Hexavalent Chromium독성에 대한 세포 보호효과
한두석,강정일,백승화,Han, Du-Seok,Kang, Jeong-Il,Baek, Seung-Hwa 대한예방한의학회 2009 대한예방한의학회지 Vol.13 No.2
Objectives : In order to evaluate the cytotoxicity of hexavalent chromium, the cytoprotective effect of phenolic compounds against hexavalent chromium-induced cytotoxicity, cell viability, cell adhesion ability, lactate dehydrogenase(LDH) activity, and morphological changes of cells were examined. Methods : We measured the cytotoxicity of hexavalent chromium with 3-[4,5-dimethyl-thiazol-2-yl]-2,5-diphenyltetrazolium bromide(MTT), 2,3-bis-[2-methoxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-caboxanilide (XTT), LDH and DPPH methods. Results : The cytotoxicity of hexavalent chromium($IC_{50}$, $44.0-51.0{\mu}M$) was high according to the toxic criteria. Cytoprotective effect of phenolic compounds against $IC_{50}$ value of hexavalent chromium in cell morphology increased in a concentration-dependent manner. Conclusions : These results suggest that 3,4,5-trihydroxybenzoic acid may be used as a cytoprotective agent against chromium(IV)-mediated cytotoxicity.
웨이트 트레이닝 모델에 의한 운동부하가 흰쥐 전경골근의 핵소체 형성부위 활성과 근섬유 형태에 미치는 효과
한두석 원광대학교 생체재료·매식연구소 1977 원광생체재료·매식 Vol.6 No.1
This study was to investigated the nucleolar organizer region(NOR) associated protein by means of silver chloride staining and muscle fiber types sections for SDH in the tibialis anterior muscle loaded with movement by klitgaard's weight training model. Ag-NOR technique is a indirect measure of the on going transcriptional activity of the rRNA gene or their activity. The number of Ag-NORs sites within nucleus may reflect protein synthetic and proliferation. Thirty spraque-Dawley adult female rat(190-200g) were divided into control and 3 experimental groups were trained for 1.2.3 months. Body weight and muscle weight measured at 1. 2. 3. month. The removed muscle fixed by immersion, and processed for light microscopy. The result were as follow, 1. Body weigh were slowly increased, in experimental group, but muscle weight were step by step increased with the lapse of time of training 2. After movement, The mean of nucleolar organizer regions per nucleus were significantly increased at 3 month in tibialis anterior muscle. 3. The percent of nucleus include 4 Ag-NOR or more was increased with the lapse of time of training. 4. In muscle fiber types, A were decreased and type C were significantly increased with the lapse of time training.
한두석,정우영,박명오,신민교,오현주,백승화,Han Du-Seok,Chung Woo-Young,Park Myung-Oh,Shin Min-Kyo,Oh Hyun-Ju,Baek Seung-Hwa 대한예방한의학회 2001 대한예방한의학회지 Vol.5 No.1
The cytotoxic activity of Cratalariae sessiliflorae on cultured NIH 3T3 fibroblasts and human oral epithelioid carcinoma cells (KB) were evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazoliumbromide (MTT) colorimetric method These fractions of methanol extract of Cratalariae sessiliflorae showed inhibitory effect in vitro in the milligram range against KB cell lines. In general, the antitumor activities of these fractions were does-dependent over the milligram range. The comparison of IC50 values of these fractions in tumor cell lines showed that their susceptibility to these fractions decrease in the following order: Fr. 4> Fr. 6> Fr. 10> Fr. 2> Fr. 11> Fr. 3> Fr. 8> Fr. 7> Fr. 9> Fr. 1> Fr. 5 by the MTT assay. These fractions were tested for their cytotoxic effects on NIH 3T3 fibroblasts using MTT assay. They exhibited potent cytotoxic activities in vitro in the milligram range against NIH 3T3 fibroblasts. In general, the cytotoxic activities of these fractions were does-dependent over the milligram range. The comparison of CD50 values of these fractions in NIH 313 fibroblasts shows that their susceptibility to these fractions in decrease the following order: Fr. 10> Fr. 9> Fr. 2 = Fr. 4> Fr. 8> Fr. 11> Fr. 1 = Fr. 7> Fr. 3> Fr. 5 = Fr. 6 by the MTT assay. These results suggests that fraction 5 has the most growth - inhibitory activity against KB cell lines.
한국산 생약으로부터 해독물질의 개발(제3보) 카드뮴으로 처리한 3T3섬유모세포에서 소엽 메탄올 분획의 수복효과
한두석,유현경,곽정숙,백승화 한국독성학회 1995 Toxicological Research Vol.11 No.2
This study was carried out to evaluate the cytotoxicity of cadmium on 3T3 fibroblast and to develop the antidote on 3T3 fibroblast which was injuried by $IC_{50}$ of cadmium. The groups for repaired effects were divided into 7 groups such as medium alone treated group. Cadmium treated $IC_{50}$ groups and 5 experimental groups $(IC_{50}$ cadmium plus $10^{-4}$ concentration of each methanol fraction). After incubation for 48 hrs in the same conditions, MTT (tetrazolium MTT), NR (neutral red) and SRB (sulforhodamine B protein) assay were measured. Light microscopic observations were also investigated. The ethyl acetate fraction of Perilla frutescens showed significantly repaired effect against cadmium cytotoxiclty and this fraction inhibited critical cell regeneration in light microscopy.
Carbofuran이 NIH 3T3섬유모세포와 흰쥐의 신장에 미치는 영향과 Phenobarbital sodium의 보상효과에 관한 연구
한두석,임요섭,한성수 한국독성학회 1997 Toxicological Research Vol.13 No.1
This study was carried out to investigate toxicity of insecticide carbofuran and compensatory effects of phenobarbital sodium (PB) in vivo and in vitro. Sprague Dawley male rats were used as experimental animals and divided into carbofuran only administered group and simultaneous application group of carbofuran and PB. At 30 rain and 1, 3, 6, 12, 24, 48 and 96 hrs after each treatment, the animals were sacrificed by decapitation. Kidney were immediately removed, immersed in fixatives, and processed with routine method for light microscopic study. Paraffin sections were stained with H-E, PAM and PAS. $5.0\times 10^4$ cell/ml of NIH 3T3 fibroblast in each well of 24 multidish were cultured: After 24 hours, the cells were treated with solution of six groups; control group cultured in media only, carbofuran $MTT_50$ or $NR_50$ group cultured in the media containing carbofuran $MTT_50$ or $NR_50$ and four experimental groups cultured in the media containing carbofuran $NR_50$ plus various concentratins of PB. After the NIH 3T3 fibroblast of all groups were cultured in same condition for 48 hours, Tetrazolium MTT (MTT) and NR (neutral red) assay were performed to evaluate the cytotoxicity of cell organelles. Under the light microscope, atrophic change of renal corpuscles were frequently observed in 1 and 2 days after carbofuran treatment. The increase of the mesangium was apparent in 1 and 2 days after carbofuran treatment. Necrotic changes of the epithelium and loss of brush border of proximal tubules were most severe at 2 and 3 days after carbofuran treatment, respectively. In contrast, there were no evidences of the toxic effects on renal tissues at 48hrs in carbofuran-PB treated groups. Carbofuran $MTT_50$ and $NR_50$ were 78$\mu M$, 82.5$\mu M$ respectively. MTT and NR quantities were significantly increased in carbofuran-PB 100$\mu M$ treatment group and carbofuran-PB 100$\mu M$ treatment group. On the basis of these results, it is obvious that PB has compensatory effects against carbofuran toxicity.