韓國産 뱀허물쌍살벌屬 2種의 營巢 習性과 個體群 發達에 關하여

鄭癸俊,李碩雨 慶尙大學校 기초과학연구소 1985 基礎科學硏究所報 Vol.1 No.-

1. 韓國産 Parapolybia屬은 P. varia에 P. indica가 追加되어 2種이 된다. 2. 두 種은 모두 直射光線이 잘 비치지 않는 山地의 숲속에 집을 짓는데, P. indica는 主로 地上 10 ㎝ 內外의 낮은 나뭇잎의 裏面에 짓고 P. varia는 보다 높은 곳의 나뭇가지에 짓는다. 3. 巢의 모양은 P. indica의 境遇는 圖形에 가까운 不正形이고, P. varia는 輻이 좁고 길다. 巢柄은 두 種 모두 巢의 土端에 位置하지만 P. indica는 板狀이고 P. varia는 棒狀이다. 4. 巢의 平均的인 방 수는 P. indica가 213個, P. varia가 298個로 P. varia의 巢가 더 컷다. 5. 두 種 모두 한마리 씩의 여왕벌에 依해 路群이 始作되며 일벌은 6月 下旬 내지 7月 上旬 傾에 처음 出現하며 P. indica는 8月 10日 傾 40마리 程度로, P. varia는 8月 中旬傾에 50마리 程度로 各各 最高에 이르고 그후 減少하게 된다. 수펄과 새 여왕벌은 두 種 모두 8月 10日 傾에 出現하기 始作하며 P. indica는 8月 下旬 傾에 50마리 程度로, P.varia는 9月 下旬 傾에 80마리 程度로 各各 最高에 達하게 된다. Nesting habits and colony development of the two paper wasps, Parapolybia indica Saussure and P. varia Fabricius were observed in the vicinity of Jinju, Kyeongsang nam do, Korea. In both 2 species, most nests were found in the forest of mountainous areas. The nests of P. indica were usually hung beneath the underside of leaves of low trees. On the other hand, nests of P. varia were discovered mostly on the higher branches of trees. The nests of P. indica were pseudomorphous or round but the nests of P. varia were narrow and long shaped. Both of the two species were haplometrotic. The first worker emergence in the two species were about the end of June or the beginning of July. The nest and population of P. varia were larger than those of P. indica. Population of worker was usually peaked in the middle August. The reproductives were peaked about the end of August and the beginning of September in P. indica and P. varia respectively.

營巢 場所에 따른 등검정쌍살벌(Polistes jadwigae Dalla Torre)의 蜂群의 發達, 寄生蟲 感染率 및 生存率에 關하여

鄭癸俊,李碩雨,金正坤 慶尙大學校 기초과학연구소 1987 基礎科學硏究所報 Vol.3 No.-

1. 등검정쌀살벌은 營巢 場所로 主로 돌 밑, 지붕의 처마 밑 等을 選好하여 植物의 줄기에도 營巢한다. 2. 蜂群의 生存率은 江邊 地域에서 가장 높은 27.3%를 나타냈고, 民家 地域에서는 20.0%, 野山 地域에서는 3.2%로 가장 낮아 棲息 場所에 따라 현격한 차이를 보여주었다. 3. 蜂群의 淘汰 原因은 江邊 地域에서는 설치류로 推定되는 動物에 의한 被害가 가장 많았으며, 民家 地域에서는 여왕벌 失踪과 사람에 의한 破壞가 가장 많았고, 野山 地域에서는 여왕벌 失踪에 의한 것이 가장 높았다. 4. 發達 段階別로 본 蜂群의 淘汰率은 세 地域 모두 幼蟲期, 踊期 順으로 높았고 卵期의 淘汰率이 가장 낮았다. 5. 寄生 昆蟲으로는 幼蟲에 寄生하는 맵시벌과의 검정무늬뾰죽맵시벌屬(Latibulus)의 1種과 巢에 寄生하는 은무늬줄명나방(Pyralis regalis) 및 세모창날개 뿔나방(Anatrachyntis japonica)의 3種이 發見되었다. Latibulus spp. 는 野山 地域의 巢에서만 나타났으며, 蜂群에 致命的 被害를 입혔다. 6. 蜂群은 4월 下旬부터 5월 上旬 傾에 1마리의 여왕벌에 의해 始作되며, 첫 일벌의 出現은 江邊 地域이 6월 16일 傾으로 가장 빨랐고, 民家 地域은 6월 20일 傾, 野山 地域은 6월 28일 傾이었으며, 繁殖蜂의 羽化 時期는 江邊 地域에서는 8월 16일 傾이었고, 野山과 民家 地域에서는 8월 23일 傾이었다. 7. 蜂群과 巢의 크기는 江邊 地域에서 가장 컸고, 野山 地域이 가장 작았다. Nesting habits, colony development, parasite infestation and colony survival rate of the paper wasp, Polistes jadwigae Dalla Torre were observed in the vicinity of Jinju, Southern Korea. Most nests were found under the stones and the eaves of houses, and some nests were found on the stems of small plants. Colony survival rates were 27.3%, 20% and 3.4% in the riverside, residential district and mountainous district, respectively. Unidentified animals, (probably rodents) attack was most serious reason of colony failure in the riverside. But, queen loss and man's destruction were most frequently occured in the residential district. In the mountainous district, queen loss was most serious. The colony failure in the course of colony development was highest in the larval stage, and the pupal stage was the next. Three species of parasite, Latibulus spp. (Hymenoptera, Ichneumonidae), Pyralis regalis Denis et Schiff. (Lepidoptera, Pyralidae) and Anatrachyntis japonica Kuroko (Lepidoptera, Ccsmopterigidae) were found in the nest. The Latibulus spp. appeared exclusively in the nests of mountainous district and seriously affected colony development. Nest Founding was initiated by a queen (haplometrotic) mostly from late April to early May. The first worker emerged in about June 16 in the riverside, June 20 in the residential district and June 28 in the mountainous district. The worker population peaked about August 20. The first reproductive emerged in about August 16 in the riverside, and August 23 in the mountainous and residential district. Colonies and nests were largest in the riverside and smallest in the mountainous district.

Comparisons of Development Potential in Bovine SCNT Embryos using Donor Cells treated with Different Demethylating Inhibitors

전병균,정계준,노규진 사단법인 한국동물생명공학회 2015 한국동물생명공학회지 Vol.30 No.3

To improve the developmental potential of bovine somatic cell nuclear transfer (SCNT) embryos, this study compared the developmental rates to blastocyst stage in the SCNT embryos using donor fibroblasts treated with 5-azacytidine (5AC) and S-adenosylhomocysteine (SAH) at different concentrations. Their reprogramming efficiency level was investigated with level of telomerase activity. Donor fibroblasts isolated from adult ear skin of a cow were exposed to 5AC and SAH at different concentrations during 2 passages. After nuclear transfer into enucleated recipient oocytes, the cleavage and developmental rates were significantly (p<0.05) decreased in the SCNT embryos using 5AC-treated fibroblasts (5AC-SCNT embryos), compared with those of non-treated control (control-SCNT embryos) and SAH-treated fibroblasts (SAH-SCNT embryos). The developmental rates to blastocyst stage tended to be slightly increased in the SAH-SCNT embryos at each of the concentrations, and especially, the developmental rates in the SCNT embryos using 1.0 mM SAH-treated fibroblasts were significantly (p<0.05) higher than that of control SCNT embryos. The mean numbers of total and ICM cell in blastocysts were also significantly (p<0.05) decreased in the 5AC-SCNT embryos, compared with those of other SCNT blastocysts. Further, the level of telomerase activity was also significantly (p< 0.05) decreased in the 5AC-SCNT embryos than those of control and SAH-SCNT embryos. Whereas, a significantly (p<0.05) up-regulated telomerase activity was observed in SAH-SCNT embryos, compare with that of control-SCNT embryos. In conclusion, SCNT embryos using hypomethylated donor cells with SAH, not 5AC, may improve the developmental potential and reprogramming efficiency.

Morphological Oogenesis in the otu1 Mutant of Drosophila melanogaster Meigen (Diptera: Drosophilidae)

김정곤,정계준 한국곤충학회 2002 Entomological Research Vol.32 No.3

The sex-linked, recessive otu1 mutant of Drosophila melanogaster was studied cytologically to reveal the effect of the otu gene mutation on oogenesis. Consequently, the germarium of the otu1 mutant was found to be larger as compared to the normal fertile one and contains many undifferentiated tumor cells resembling cystocytes. Each ovary consists of three types of ovarioles; ovarioles with only a germarium, ovarioles with a germarium and one tumor chamber, and ovarioles with a germarium and two tumor chambers. In the otu1 germarium, the mesodermal follicle cells invade the clusters of different number of tumor cells at the posterior region of the germarium in order to make the tumor chamber and the dividing frequency of the tumor cells in the .rst tumor chamber was six times more than in the second tumor chamber. Follicle cells surrounding the tumor chamber are morphologically normal, but the number of the follicle cells becomes less as the tumor chambers move down contrary to the fertile one. The otu1 mutant female never lays the egg and the formation of the vitelline membrane is incomplete. The otu1 mutant tumor cells also showed characteristics of certain rapidly dividing cells and its nucleus is lobulated.

5,6차 敎育課程에 의한 中學校 科學 1 敎科書 중 生物 領域에 대한 比較 分析

황선원,정계준,김정곤 경상대학교 과학교육연구소 1996 科學敎育硏究所報 Vol.16 No.1

The biology area of the 8 science textbooks 1 of the middle school by the 6th curriculum were analyzed comparatively with those of the 5th curriculum. 1. The old curriculum presented a comprehensive object on each major subject and detailed target goals for each sub-theme. While the new curriculum point on the research activity. The school year objective in the old curriculum was deleted in the new textbooks. 2. The new curriculum emphasizes research activities. It also give emphasis to the biological material in our surroundings, field tour studies, open questions, and various active applications and participation in scientific events. 3. The degree of emphasis for each scientific area varies in the eight new textbooks according to publisher. The number of pages allotted for biological subjects is 28.4% and takes up more pages than other areas (earth science, 25.6%; chemistry and physics, 23%). Experimental observation and research in biology are discussed at 35.7% and 24.6%, respectively. In the old textbooks, 29.1% of the total pages dedicated to biology and experimental observation and research occupied 36.4% and 34.2, respectively. In these points, these are little differences between the new and the old textbooks. 4. The biological content dealing with levels area comprises 68.7% of the new textbooks and 62.8% of the old. Biological structure and function are discussed 55.1% in the new textbook, as opposed to 48.3% in the old. The least emphasis is on cells (4.7% vs 5.5%) as well as the topic areas of level and evolution (0.4% vs 0.5%). Molecules, individual groups, hereditary genetics, controls and stabilizations are not mentioned at all in the new and the old textbooks. 5. Experimental observation in the new textbooks are 19.1 times, which is greater than the average 15.8 in the old. The most common experimental aspects are shape observation and similarities of many organisms in the new and the old textbooks. Common experimental themes are cell observation, the appearances of mold, froggy, grasshoppers, crucial carps and crayfish. The themes of other experimental observations are usually similar even though there are some differences in experimental materials. 6. Fifty-five species of living organisms are indicated in the new textbook, as opposed to 39 in the old. Twenty-five organisms such as crayfish, frogs and forsythe are discussed in common in the new and the old textbooks. The most frequency mentioned material in the new and old textbooks is the peach tree (14 times vs. 7 times, respectively). 7. Eleven species of chemicals are used for experimental observations in the new textbook, and seven species in the old textbook. Common chemicals used in both textbooks are ethanol and ether. Chemicals used more than 5 times in the new textbooks are hot water, anesthetics, Chinese ink and pond water. Scissors and sixty-nine other species of instruments used for experimental observations, in the new textbook and filtering paper and twenty-nine other species are found in the old textbook. 8. Drawings appear on average about 44.1 biological pistures per book in the new textbooks and 66.2 pictures per book, in the old textbooks. There are also about 13,9 photos in the new vs. 17.8 photos in the old. 9. The results of the comparison of the former textbooks with the new in regard to the biological subject matter can be summarized as follows: the understanding of scientific facts, concepts and principles were emphasized in the old textbooks, while the new textbooks encourage interest in scientific study by learning basic research methods, understanding natural phenomena through research activity and development of a research attitude in students. 10. Suggestions for future revisions of the textbook: If we reduce memorized vote learning and focus on individualized self-motivated learning, one can expect many results. This method conjoined with free discussions can further enhance results. Furthermore, when selecting topics and studies, research activities should be emphasized. These methods will raise the students' intellectual curiosity more than the textbooks centered learning systems.

The Regulatory Role of Erythropoietin : In Vivo and In Vitro Studies

KIM, JUNG KON,KWACK, DAE OH,JEONG, GIE JOON 경상대학교 유전공학연구소 1985 遺傳工學硏究所報 Vol.4 No.-

Friend Murine Leukemia Virus (F-MuLV) is affective against 2-day old newborns in NFS/n Swiss mice. Infected babies suffer from splenomegaly and anemia at the age of 4-5 weeks beyond which the condition worsens and is fatal. However, the spleen size can be reduced by hypertransfusion for 8 weeks. If left alone after hypertransfusion, the spleen enlarges and becomes palpable again. This time the spleen cells are transplantable and inflict the same disease if injected to adults. The transplantable cells are kept alive by sequential passage through healthy NFS/n Swiss mice. In this way 8 transplantable lines were developed. The transformed cells demonstrated to be erythropoietin(EPO) responsive in culture when grown in methyl-cellulose medium. These cells did not lose their transplantability for they were capable of inflicting the same disease in adults after in vitro culture. In vivo eryrhropoietin helps in the proliferation of transformed cells and kills the animals faster while hypertransfusion prolongs the life of an infected mouse. Influence of EPO on the in vivo growth of leukemic cells was indicated that administration of the hormone caused a more severe leukemia and rapid death, while transfusion of red blood cells, which lowers endogenous EPO, lead to decreased spleen size and increased survival of leukemic mice.

Cytological Studies on Yolk Formation of Drosophila melanogaster

Kim, Jung Kon,Kwack, Dae Oh,Jeong, Gie Joon 慶尙大學校 기초과학연구소 1986 基礎科學硏究所報 Vol.2 No.-

난황형성과정을 프라스틱에 포매시킨 난소를 절단하여 관찰한 결과 난소의 여포상피세포는 초파리의 난황형성에 중요한 기능을 가졌다는 것이 밝혀졌고, 난황물질의 합성과 난황구의 형성은 여러단계의 복잡한 과정이다. 난황축적과정의 형태적변화는 난막의 함입과 미세소관이나 미세한 과립이 난세포와 여포상피세포의 접촉면에서 형성되는 것이다. 이들 소과립이 난의 주연부에서 서로 결합하여 점차 큰 난황과립이 되었으며 난의 내부로 가면서 점차 전자밀도가 증가하였고 밀집하여 성숙된 낭황이 형성되었다.

과학교사 재교육의 개선방안

김정곤,김인호,정계준,김봉곤,구인선 한국과학교육학회 1991 한국과학교육학회지 Vol.11 No.1

The purpose of this study is in making a important way for in-service training of science teacthers. So the study was carried out to analyze the text books of in-service training school in Gyeongnam, Kyungpook, Junpook province and Pusan city and to survey teacher's opinion by questonnaires collected from teachers in Gyeongnam province. The result abtained are as follows; 1. The contents of the text books for in-service training course have very critical difference at each province. 2. Most of teachers wanted to get more updating information of the science and more comprehensive knowledge of higher level of science subject. 3. In order to obtain comprehensive basic concept, develop of inquiry ability, and promote of scientific attitude, the thinking experiment which is selected to experimental subjects of secondary school are need to be selected. 4. The new text-book model for in-service training course should be developed, and the finnancial support will be required to develop such a text.

LHR 조건하에서 배양 인체 백혈구가 나타내는 SCE 와 excision DNA repair 능력

이광호,이석우,이승길,지성길,정계준,원태웅,박현숙 한국유전학회 1987 Genes & Genomics Vol.9 No.3

Go human lymphocytes were treated prior to culture initiation with 7 cytostatics (MMC, CLM, MTX, Ara-C, Act-D, HU, 5-FU) in order to determine whether cytostatics having different mode of action on DNA are equally effective in inducing DNA lesions in Go human lymphocytes. Go lymphocytes damaged with cytostatics showing positive results (MMC, CLM) were allowed to repair DNA damages under LHR conditions for 12-36 hr to compare excision DNA repair capacity in exposed lymphocytes. Additionally, we carried out a series of experiments to investigate the relevance of Go exposure duration (1-4 hr), SCE induction and excision repair capacity during limited LHR period (12 hr), the effect of BUdR on excision DNA repair activity in Go lymphocytes under LHR conditions and the characteristics of the unexcision repaired lesions remained in the DNA of Go lymphocytes after LHR that can lead to SCE formation, MMC and CLM induced exposure duration-related increase in SCEs, while MTX, Ara-C, Act-D, HU and 5-FU showed only minor variations in SCE indutions during Go exposure of 1-4 hr. There was a significant difference between SCEs after 2 hr of MMC/CLM treatment. and those after 12-36 hr LHR conditions. The effect of limited LHR period (12 hr) on SCE value was not dependent on the exposure duration or induced SCE frequencies. In cultures exposed to CLM, BUdR induced LHR period-dependent increase of SCEs during LHR period. LHR-allowed lymphocytes showed more rapid decrease of SCE as compare to those of cultures without LHR conditions. MMC-induced SCEs were more slowly decreased than CLM-induced SCEs with or without LHR conditions.

Comparison of mesenchymal stem cells isolated from various tissues of isogenic mini-pig

전병균,Dinesh Bharti,이원재,장시정,박지성,정계준,노규진 한국통합생물학회 2015 Animal cells and systems Vol.19 No.6

The present study compared the cellular properties based on cell surface differentiation markers, telomerase activity, stem cell-specific transcripts and differentiation capacity into adipocytes, osteocytes and neurocytes in multipotent mesenchymal stem cells (MSCs) isolated from bone marrow (BMSCs), adipose (ASCs), ovarian (OSCs), muscle (MuSCs) and skin (SSCs) tissues of the same donor mini-pig. Using flow cytometry, all isolated MSCs expressed stem cell-positive surface markers CD29, CD44 and Vimentin, at a high level. Using reverse transcription-polymerase chain reaction, BMSCs, ASCs and SSCs showed higher expression of stem cell-specific transcripts (NANOG, OCT-4 and SOX-2) as compared to OSCs and MuSCs. Telomerase activity was detected by relative-quantitative telomerase repeats amplification protocol at a similar level in all MSCs. Further, analysis of cytochemical staining and lineage-specific transcripts demonstrated that all MSCs get easily differentiated into adipocytes, except for OSCs, whereas, BMSCs and MuSCs easily differentiated into osteocytes, compared to ASCs, OSCs and SSCs. Furthermore, all MSC groups showed the same level of capacity for neurocytes differentiation. Based on these results, the cellular properties were dominantly expressed in BMSCs, ASCs and SSCs, whereas the differentiation capacity was dominantly expressed in BMSCs and MuSCs, compared to others MSCs. Taken together, BMSCs could potentially be used as a good source of MSCs for clinical application and fundamental stem cell research.