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이혁,윤문철,김종도 한국기계가공학회 2020 한국기계가공학회지 Vol.19 No.11
The forced vibration characteristics for two impulse forces with time lag was discussed in the vehicle dullprogress model. Detailed numerical analyses of the time domain were performed systematically. By the twoexciting impulse forces, the responses of displacement, the velocity, and the acceleration were investigated indetail for the vehicle’s vibration. Notably, the forced vibration responses in the time domain can be used toidentify and monitor several vehicle vibration models. In order to define the responses of displacement, thevelocity, and the acceleration, we applied a numerical technique (i.e., the Runge–Kutta–Gill method[1,2]). These variables were subsequently used to analyze the vehicle’s vibration according to the time lapse andwhile it passed over a bump stock; moreover, the characteristics of the variables were analyzed in detailaccording to their force conditions. Finally, the intrinsic characteristics of the forced vibration were discussedin the context of the automobile model. Overall, our results indicate that the tested method can besuccessfully applied under different damped conditions.
이혁,박정호,박동일,김홍주,조용균,손정일,전우규,김병익,채승완 대한소화기 기능성질환∙운동학회 2013 Journal of Neurogastroenterology and Motility (JNM Vol.19 No.2
Background/Aims Although mucosal mast cell tryptase is known to significantly increase intestinal permeability, the relationship between mucosal mast cells and intestinal permeability remains unclear. The objective of this study was to evaluate the correlation among intestinal permeability, tryptase activity and mucosal mast cell count. Methods Rectal biopsies from 16 patients with diarrhea-predominant irritable bowel syndrome (IBS-D) and 7 normal subjects were assessed for tryptase activity and macromolecular permeability using horseradish peroxidase in Ussing chambers. In addition, mucosal mast cell levels were immunohistochemically quantified via image analysis. Results Rectal biopsy of tissues from IBS-D patients showed significantly increased permeability compared with those from normal controls (0.644 ± 0.08 and 0.06 ± 0.00 ng/2 hr/mm2, P < 0.01). Tryptase activity was also substantially higher in rectal biopsy samples from IBS-D patients than those from normal controls (0.86 ± 0.18 and 0.28 ± 0.04 mU/mg protein, P < 0.05). Mucosal mast cell counts were not significantly different between the 2 groups (P > 0.05). However, correlation analysis revealed that only mucosal mast cell count was significantly correlated with intestinal permeability in IBS-D patients (r = 0.558, P < 0.05). Conclusions This study demonstrated a positive correlation between the number of mucosal mast cells and intestinal permeability, suggesting that mucosal mast cells play an important role for increased intestinal permeability in patients with IBS-D.
Immunocapture RT-PCR을 이용한 박과작물 종자전염 바이러스의 검출
이혁인,김정희,예미지 한국식물병리학회 2010 식물병연구 Vol.16 No.2
Immunocapture reverse transcription polymerase chain reaction (IC-RT-PCR) was applied to the detection of Cucumber green mottle mosaic virus (CGMMV), Kyuri green mottle mosaic virus (KGMMV), and Zucchini green mottle mosaic virus (ZGMMV) on Cucurbitaceae crops. These seed-borne tobamoviruses were accurately detected from the infected leaves and seeds by IC-RT-PCR. This method was estimated to be about 100 times more sensitive than ELISA, and also it allowed the direct confirmation of ELISA results by using the captured antigens from a completed ELISA microwell. This convenient and reliable method could be used routinely for large-scale field surveys or seed tests of Cucurbitaceae crops. 박과작물에 발생하는 종자전염 바이러스 3종(CGMMV, KGMMV, ZGMMV)에 대한 검출을 위해 IC-RT-PCR의 적용 가능성을 시험하였다. IC-RT-PCR을 이용하여 감염 종자와 잎으로 부터 바이러스의 특이적인 검출이 가능하였으며, 검출 민감도는 ELISA 보다 100배 이상 높았다. 또한 반응을 마친 ELISA 마이크로플레이트에 남아있는 항원을 IC-RT-PCR의 template로 사용할 경우 ELISA 결과를 곧바로 확인할 수 있었다. 따라서 IC-RT-PCR에 의한 본 검정방법은 박과작물의 대규모 포장검사 및 종자검사에 편리하게 사용될 수 있을 것으로 기대된다.