도인승기탕에 의한 당뇨병성 혈관장애 개선효과

이윤정,김언국,김혜윰,윤정주,이소민,이용표,이건목,강대길,이호섭,Lee, Yun Jung,Kim, Eon Kuk,Kim, Hye Yoom,Yoon, Jung Joo,Lee, So Min,Lee, Yong-Pyo,Lee, Geon Mok,Kang, Dae Gill,Lee, Ho Sub 대한한의학방제학회 2013 大韓韓醫學方劑學會誌 Vol.21 No.1

Objectives : Diabetes mellitus is the leading cause for vascular complications such as atherosclerosis. The present study is to investigate whether Doinseunggi-tang (DST) improves diabetic vascular dysfunction in type II diabetes. Methods : The db/db mice were treated with high fat/high cholesterol diet and DST (200 mg/kg/day) for 8 weeks. Results : DST significantly lowered blood glucose and systolic blood pressure. In addition, DST also markedly decreased total plasma cholesterol, triglyceride, and LDL-cholesterol, whereas increased the HDL-cholesterol. Vascular relaxation of aortic rings by acetylcholine or SNP was ameliorated by DST in a dose-dependent manner. Damage of vascular intima and hypertrophic of media was improved by DST. Immunohistological study revealed that DST attenuated the increase of ICAM-1, VCAM-1, and ET-1 expression in thoracic aorta. Conclusions : Taken together, DST suppressed hyperglycemia and diabetic vascular dysfunction in type II db/db mice. The present data suggests that Doinseunggi-tang may be prevent a development of diabetic atherosclerosis.

고지혈증 동물모델에서 홍삼과 천마 혼합투여에 의한 혈관 염증 개선연구

이윤정,김혜윰,윤정주,이소민,고정현,이호섭,최경민,강대길,Lee, Yun-Jung,Kim, Hye-Yoom,Yoon, Jung-Joo,Lee, So-Min,Kho, Joung-Hyun,Lee, Ho-Sub,Choi, Kyung-Min,Kang, Dae-Gill 대한한의학방제학회 2012 大韓韓醫學方劑學會誌 Vol.20 No.1

Objectives : This experimental study was designed to investigate the inhibitory effects of combination with Korean red ginseng and Gastrodia rhizoma on vascular dysfunction in high-fat/cholesterol diet-induced hyperlipidemia. Methods : Sprague-Dawley rats were fed with 7.5% cocoa butter and 1.25% cholesterol for 10 weeks, with Panax ginseng (PG), and mixtures of Panax ginseng and Gastrodia rhizoma (PGM), respectively. Results : Chronic treatment with PG and PGM significantly decreased body weight. The aortic expression of cell adhesion molecules such as intracellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and E-selectin were markedly increased in hyperlipidemia rats. Interestingly, PGM significantly decreased cell adhesion molecules expression. However, there was no significant decrease in PG group. In addition, PG and PGM group inhibited high-fat/cholesterol diet-induced cytokine such as monocyte chemoattractant protein (MCP-1) mRNA expression. Furthermore, PG and PGM group significantly decreased c-reactive protein protein (CRP) level. Especially, PGM significantly accentuated the decrease of MCP-1 mRNA expression and CRP level. Conclusions : the present study provides an evidence that combination with Panax ginseng and Gastrodia rhizoma enhances anti-vascular protective effects through suppression of vascular inflammation in hyperlipidemic rats.

Salmonella typhimurium 에서 무산소 환경 유도유전자들에 대한 조절유전자들의 특성 및 클로닝

이윤정,방일수,이인수,이영록,박용근 ( Yun Joung Lee,Iel Soo Bang,In Soo Lee,Yung Nok Lee,Yong Keun Park ) 한국환경생물학회 1993 환경생물 : 환경생물학회지 Vol.11 No.2

New regulatory loci which participate in the anaerobic environment inducible gene(ani) expression in Salmonella typhimurium were identified. These regulators (oxr) showed negative regulatory effect on the anaerobiosis inducible operon fusions of this organism in aerobic environment (oxr 107 and oxr 108 to ani 2001, oxr 109 to ani 2007). They were identified as the trans-acting regulators, and increased β-galactosidase activity of ani-lacZ fusion strains up to about 10-240 fold in aerobic environment. By using the library of S. typhimurium chromosomal DNA, two negative regulators (oxr 101 and oxr 109) were cloned. The insert fragment of each clone, pPK32 for oxr 101 and pPK33 to oxr 109 were 7.8Kb and 9.3Kb, respectively. The recombinant plasmid pPK32 didn`t complement the mutation effect of oxr 101 on minimal media but rich media, pPK33 complemented the mutation effect of oxr 109 on both minimal and rich media.

시민참여형 과학기술 거버넌스의 논의와 실험에 내재된 도구적 관점: 영국 공론화 사례 중심

이윤정,Lee, Yunjeong 기술경영경제학회 2015 Journal of Technology Innovation Vol.23 No.2

지난 30여 년간 서구 민주주의 사회를 중심으로 시민참여형 과학기술 거버넌스에 대한 논의와 실험이 다양하게 이루어졌다. 이에 대한 당위성에는 사회적 공감대가 형성되었지만 이를 통해 궁극적으로 얻고자 하는 목표와 방법론은 다양했다. 본 논문에서는 겉으로 주창되는 규범적, 실제적 이유와는 달리 은밀히 내재된 도구적 관점의 시민참여 논리가 갖는 정책적 함의를 고찰한다. 사례로 영국의 시민참여형 과학기술 거버넌스 담론의 형성과정과 실험을 분석한다. 1990년대 후반부터 제도권의 다양한 정책관련 기관들이 주장한 시민참여형 과학기술 거버넌스의 필요 논리에 이용된 쟁점 및 실제로 시행된 두 공론화 과정(유전자 변형 작물의 상업화 관련 GM Dialogue와 방사성 폐기물 처리 방법 관련 CoRWM의 프로그램)에 드러난 도구적 관점을 분석한다. 이를 통해, 시민참여형 과학기술 거버넌스로의 전환 시도에는 겉으로 주창된 민주주의적 의사결정과 창의적 문제해결의 목표 외에, 이해관계자들이 자신의 이해 실현을 위해 도구로서 이용하려는 은밀한 시도들이 있었음을 보여준다. Various concepts and practices of public deliberation have been exercised over the last three decades in western countries for science and technology (ST) governance. There has been a general social consensus for the need of such new governance notwithstanding, ends and means to achieve it varied. Among various rationales, while normative and substantive perspectives are explicitly claimed in public as reasons for public engagement in ST policy making, instrumental perspectives tend to be implicitly arranged by interested parties and still affecting policy significantly. This paper therefore, examines the implications of such instrumental perspectives in public engaged ST governance. To do so, this paper analyses the discourses and practices of public deliberation for ST governance in the UK from the late 1990's until the mid 2000's. It examines the proposals made by various policy institutions and two real public deliberations- the GM Dialogue for commercialization of genetically modified crops and the Committee on Radioactive Waste Management(CoRWM)'s programme. This study finds that policy institutions tried to employ public deliberation as strategic instrument for their policy interest, which contrasted with their outwardly claimed rationales of democratic policy making and better quality in decision for ST governance.

의료계는 지금-간호사의 해외진출

이윤정,Lee, Yun-Jeong 대한간호협회 2006 대한간호 Vol.45 No.4

비중격만곡증 환자의 눈물주머니코안연결술의 임상예후

이윤정,문상원,조희윤,정진혁,Yoon Jung Lee,M,D,Sang-Won Moon,M,D,Hee Yoon Cho,M,D,Jin-Hyeok Jeong,M,D 대한안과학회 2008 대한안과학회지 Vol.49 No.5

Purpose: Septal deviation has few subjective symptoms. However, a difficulty remains in doing DCR (dacryocystorhinostomy) in these patients. We investigate the clinical course of DCR in patients with a septal deviation. Methods: Acquired nasolacrimal duct-obstructed patients who had undergone DCR with septal deviation from 2003 to 2006 in our hospital were retrospectively analyzed. We measured the septal distance using paranasal computed tomography taken preoperatively and compared the sex and age of patients, the kinds of operations, outcome of surgery, and ocular and nasal complications in our analysis. Results: Twenty-two eyes of 22 patients with a mean age of 55.3 years (range 29~74) were analyzed (4 males, 18 females). Mean septal distance was 4.84 mm (range 2.18~6.99 mm). Nineteen eyes had endonasal DCR, and three had external DCR. Two had DCR combined with septoplasty. Three had septoplasty as a secondary operation, Nasal complication was synechiae in 9 eyes. DCR success was seen in 15 eyes, and DCR failure in 7 eyes. Septal distance was not correlated with DCR success but was correlated with nasal complication (Mann-Whitney test, p=0.003) Conclusions: In the case of DCR with septal deviation, the surgical technique used will need to minimize the mucosal injury and ensure appropriate nasal correction to prevent future complications.

균형측 교합장애로 인한 하악운동 및 저작근 활성도의 변화에 관한 연구

이윤정,박남수,최부병,Lee, Yun-Jeong,Park, Nam-Soo,Choi, Boo-Byung 대한치과보철학회 1998 대한치과보철학회지 Vol.36 No.4

The purpose of this study was to investigate the influence of balancing interference on the mandibular movement and masticatory muscle activity. 5 subjects(male. average age of 24.3) without dysfuction in masticatory system were selected. The balancing interference was provided by construction of cast metal crown and onlay on the upper and lower first molars. Clinical examination, changes displacement and velocity, and muscle activity were recorded and analyzed by means of BioPak system(Bioresearch Inc., Milwaukee Wisconsin. USA). The results were as follows ; 1. In clinical examination, various symptoms were reported by all subjects after application of interference. Almost symptoms were subsided after elimination of interference. 2. In the border movements in frontal plane, lateral border movement toward non-interference side was changed according to the interference after application of interference. Immediately after removal of interference, border movements' pattern was recovered as same as before experiment. 3. During gum chewing on the non-interference side, horizontal movement was decreased immediately after application of interference(p<0.05). 1 week after application of interference, horizontal movement was more decreased in 3 subjects and showed a chopping type masticatory stroke. But in 2subjects, horizontal movement was increased to avoid interference. 4. In EMG of the mandibular rest position, no significant changes were showed in the experiment period(p>0.05). 5. During gum chewing on the interference side, the activity of opposite temporal muscle was increased immediately after application of interference(p<0.05). 1 week after application of interference. The activity of ipsilateral temporal muscle and left and right masseter muscles was increased (p<0.05). 1 week after elimination of interference, increased muscle activity was recovered about the same level as before experiment. 6. During gum chewing on the non-interference side, 1 week after application of interference, the activity of ipsilateral temporal muscle was increased (p<0.05). 1 week after elimination of interference. increased muscle activity was returned about the same level as before experiment.

경계 차감 클러스터링에 기반한 클러스터 개수 추정 화자식별

이윤정,최민정,서창우,한헌수,Lee, Youn-Jeong,Choi, Min-Jung,Seo, Chang-Woo,Hahn, Hern-Soo 한국음향학회 2007 韓國音響學會誌 Vol.26 No.5

본 논문에서는 화자식별을 위한 특징벡터의 새로운 클러스터링 방법을 제안한다. 제안된 방법은 클러스터 센터에 대한 초기값 설정과 클러스터 개수에 대한 사전 정보 없이 클러스터링이 가능하다. 각 클러스터 센터는 경계 차감 클러스터링 알고리즘으로 한 번에 한 개의 클러스터 센터가 추가됨으로써 순차적으로 구해지며, 클러스터 개수는 클러스터간의 상호관계를 조사하여 결정된다. 인공 생성 데이터 및 TIMIT 음성을 이용하여 실험한 결과로부터 제안된 방법이 기존의 방법보다 우수함을 확인하였다. In this paper we propose a new clustering algorithm that performs clustering the feature vectors for the speaker identification. Unlike typical clustering approaches, the proposed method performs the clustering without the initial guesses of locations of the cluster centers and a priori information about the number of clusters. Cluster centers are obtained incrementally by adding one cluster center at a time through the boundary subtractive clustering algorithm. The number of clusters is obtained from investigating the mutual relationship between clusters. The experimental results for artificial datum and TIMIT DB show the effectiveness of the proposed algorithm as compared with the conventional methods.

홍삼가공식품의 식품유형별 및 제형별 진세노사이드 함량 비교

이윤정,장민수,이인숙,김현정,장현정,황인숙,Yun-Jeong Yi,Min-Su Chang,In-Sook Lee,Hyun-Jeong Kim,Hyun-Jeong Jang,In-Sook Hwang 한국식품위생안전성학회 2024 한국식품위생안전성학회지 Vol.39 No.2

서울 시내 유통점과 온라인 쇼핑몰에서 구입한 홍삼함유식품 66건과 건강기능식품 홍삼제품 35건의 기능 성분인 진세노사이드 함량을 비교·분석하였다. 홍삼함유식품 66건 중 전통시장에서 구입한 2건은 진세노사이드가 검출되지 않았고 64건의 제품에서는 진세노사이드 함량에 대한 기준은 없지만 일일 섭취량 기준 0.55-71.56 mg을 함유하고 있었다. 건강기능식품의 홍삼제품은 진세노사이드 평균 함량이 분말형 제품을 제외하고 일일 섭취량 기준 18.23 mg으로 홍삼함유식품의 8.80 mg 보다 약 2배 정도가 많았다. 홍삼과 흑삼 비교 시 진세노사이드 함량은 홍삼 제품이 흑삼 제품보다 많거나 비슷하였으나 분말형 제품의 경우 흑삼 제품이 2배 많았다. 제형별 진세노사이드 평균 함량은 일일섭취량 기준 농축액 21.95 mg으로 가장 많고 분말 형태 12.54 mg, 스틱형 7.36 mg, 파우치형이 4.10 mg 순이었다. 흑삼제품은 고기능성으로 알려져 있으나 대부분 일반 식품으로 판매되고 있어 건강기능식품 수준의 관리와 규제가 필요할 것으로 보이며 흑삼의 규격기준 설정 이후에는 흑삼제품의 기능성분 함량 재평가가 필요할 것으로 판단된다. 스틱형이나 농축액 제품의 경우 건강기능식품뿐만 아니라 일반식품인 액상차, 홍삼음료로도 널리 판매되고 있어 식품유형 표시를 명확히 해야 할 것으로 보인다. 최근 면역력 향상을 위한 소비자의 관심 증가로 다양한 건강기능식품의 수요가 늘어나고 있다. 따라서 홍삼을 함유한 식품을 섭취 목적에 맞게 선택할 수 있도록 정확한 함량정보 제공을 위해 다양한 제품에 대한 지속적인 품질평가가 필요하다. Red ginseng is manufactured as a health-functional food and is also present in various food types and in different product forms. However, there is currently no standardized regulation of ginsenoside content in foods containing red ginseng. In the present study, we analyzed the ginsenoside content of 66 red ginseng-containing foods and 35 health-functional foods collected online and directly from the market. The ginsenoside content was assessed using liquid chromatography (LC) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) methods. The ginsenoside content of the various food types ranged 0.0 (not detected)-71.567 mg per daily intake of foods containing red ginseng. Sugar-preserved foods had the highest ginsenoside content, followed by solid teas, liquid teas, and red ginseng beverages. For health-functional foods, the ginsenoside content ranged 3.4-58.5 mg per daily intake, with levels ranging 83-607% of the indicated amounts. All values met the established standards. Upon comparing red ginseng health-functional foods and red ginseng-containing foods, the average ginsenoside content was determined to be 18.21 and 8.79 mg, respectively, thus being nearly twice as high in health-functional foods. However, there was a minimal difference between the ginsenoside content of red and black ginseng, with values of 11.84 and 12.63 mg, respectively. These findings provide insights on the variations in ginsenoside content of red and black ginseng in various food forms. This information is expected to be valuable for future regulations and consumer choice of products containing red ginseng.

근·현대사 이후 한국인의 최다 선호 일반의약품의 추이에 대한 연대별 분석 및 종합적 의의에 대한 평가

이윤정,강태진,임성실,Lee, Yun Jeong,Kang, Tae Jin,Lim, Sung Cil 대한약학회 2016 약학회지 Vol.60 No.3

Over-the-counter (OTC) drugs refer to medicines that are generally safe when used according to the product label. We aimed to assess and reflect upon changes in perception of health and health-related demands by decades in Korea according to the consumption and sales trends of OTC drugs. This study was conducted by literature search on the production and sale rankings of OTC drug market in Korea. Changes in the OTC drug market were analyzed and organized by decades to evaluate changes in drug demands and the influence of national and societal factors. There was a specific trend in the most popular drugs by decades. In the 1950s, drugs of top necessity were antibiotics and helminthics. In the 1960s, the pharmaceutical industry quickly grew and invigorators, such as Bacchus$^{(R)}$, Alps$^{(R)}$, Aronamin$^{(R)}$, were top manufactured drugs. Popularity of these invigorating drinks and vitamin products continued until the 1990s. In 1990s, sales of topical nonsteroidal anti-inflammatory drugs (Ketotop Plaster$^{(R)}$, Trast Patch$^{(R)}$), and in 2000s, gum disease medicine (Insadol$^{(R)}$) and liver and intestine supplement (Ursa$^{(R)}$) were prominent. However, after the separation of prescribing and dispensing in 2000, the sales of OTC drugs decreased dramatically from 58.7% of the total market share in 1990s to 39.6% in 2000 and this trend has continued. In 2012, thirteen OTC drugs were allowed to be sold in convenience stores, and as the sales of health functional foods have been expanding beyond pharmacies, sales of invigorators and nutritional supplements in pharmacies have continued to decrease. As government's drug expenditure will continue to grow, reclassification of OTC drugs based on established safety information and deliberate team efforts on continued development of OTC drugs to meet the health demands of Koreans are required by the healthcare professionals, pharmaceutical industries, and the government.