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LG AdvanSure TB/NTM Real-Time PCR Kit의 항산균 진단 수행 능력 평가
황상선,어영,오기진,장인호,윤갑준,김효열,김영근 대한임상미생물학회 2011 Annals of clinical microbiology Vol.14 No.2
Background: The AdvanSure TB/NTM real-time PCR kit (AdvanSure) was newly developed in Korea to detect Mycobacterium tuberculosis and nontuberculous mycobacteria (NTM) utilizing a specific primer and TaqMan probe targeting the IS6110 and rpoB genes which are unique to these species. The purpose of the present study was to evaluate the clinical utility of AdvanSure by comparing the results of acid-fast staining, mycobacteria culture, COBAS Amplicor MTB PCR (Amplicor), and AdvanSure. Methods: A total of 182 specimens (105 respiratory and 77 nonrespiratory specimens) were obtained from 165 patients, and acid fast bacilli (AFB) staining, mycobacteria culture, and Amplicor were performed on all specimens. AdvanSure was also performed on the above specimens using the SLAN real-time PCR detection system. The sensitivity and specificity of AdvanSure were analyzed using AFB staining and culture. Results: Of the 182 specimens, M. tuberculosis was detected in 43 specimens and NTM was detected in 12 specimens according to PCR and/or culture. The sensitivity and specificity of the AdvanSure based on AFB culture were 97.3% (36/37) and 95.5% (127/133) in M. tuberculosis and 75.0% (9/12) and 100% (0/133) in NTM, respectively. Conclusion: AdvanSure could be useful for detecting M. tuberculosis and NTM in the clinical laboratory with high sensitivity and specificity.
어영,홍주희,오기진,조현미,박순덕,김주원,이환종,최은화,윤갑준,이진아 대한진단검사의학회 2013 Annals of Laboratory Medicine Vol.33 No.6
Background: This study aimed to evaluate the prevalence of Mycoplasma pneumoniae in primary and tertiary care hospitals and its macrolide resistance rate. Methods: Nasopharyngeal swabs were collected from 195 pediatric patients in primary and tertiary care hospitals from October to November 2010. The AccuPower MP real-time PCR kit (Bioneer, Korea) was used for the detection of M. pneumoniae. Direct amplicon sequencing was performed to detect point mutations conferring resistance to macrolides in the 23S rRNA gene. Results: Among the 195 specimens, 17 (8.7%) were M. pneumoniae positive, and 3 of the strains (17.6%) obtained from these 17 specimens displayed the A2063G mutation in 23S rRNA. Three macrolide-resistant M. pneumoniae isolates were isolated from patients hospitalized at the primary care hospital. The positive rates of M. pneumoniae for the primary and tertiary care hospitals were 12.1% (15/124) and 2.8% (2/71), respectively (P=0.033). Conclusions: The positive rate of M. pneumoniae in the primary care hospital was higher than that in the tertiary care hospital. Simultaneous detection of M. pneumoniae and macrolide-resistant mutation genes in the 23S rRNA by real-time PCR is needed for rapid diagnosis and therapy of M. pneumoniae infections.
Two Cases of Mycoplasma pneumoniae Pneumonia with A2063G Mutation in the 23S rRNA Gene in Siblings
홍주희,천진경,어영,오기진,김주원,윤갑준 대한진단검사의학회 2013 Annals of Laboratory Medicine Vol.33 No.1
We describe 2 cases of pneumonia caused by the same macrolide-resistant Mycoplasma pneumoniae in siblings. M. pneumoniae was identified using real-time PCR. Direct sequence analysis of the 23S rRNA gene revealed a point mutation in V domain (A2063G) of the 23S rRNA gene.
주권선 전류검출에 의한 단상유도전동기의 구동시스템 구현
최낙일(N.I Choi),박수강(S.K Park),오금곤(K. G Oh),백형래(H.L Baek),임양수(Y.S Lim),오기진(K.J Oh) 전력전자학회 1997 전력전자학술대회 논문집 Vol.1997 No.-
A Single phase induction motor is the predominant fractional horsepower power rated, extensively used in domestic and industrial applications. For example, these motors provide the motive power to washing machines, fans, refrigerators, etc. In this paper, single phase induction motor input current was detected by a small current transformer and determined TRIAC gate signal by using op-amp analog circuit. The soft-start strategy is based on limited auxiliary current angle during starting period in the closed-loop control. The Simulation and experimental result show the motor's starting and are compared with centrifugal switch starting motor.