『여창가요록』 양승민본의 문헌적 특징과 자료적 가치

양승민 ( Seung Min Yang ) 한국시가학회 2012 韓國 詩歌硏究 Vol.33 No.-

이 글은 새로 발굴한 필자 소장본 『여창가요록』을 소개, 고찰한 논문이다. 『여창가요록』 양승민본은 1853년도에 雲谷先生이라는 사람이 鶴仙娘子라는 京妓에게 필사해준 가집이다. 현전 『여창가요록』 중 最古本에 해당하는 이본이다. 늦어도 1853년 이전에 편찬되었기에, 『가곡원류』의 초창기 형성 과정을 보여주는 자료이다. 양승민본 『여창가요록』은 후대 『가곡원류』가 갖춘 기본적 체재를 두루 구비한 이본이다. 따라서 여창 중심으로 편찬된 책이긴 하나 『가곡원류』의 한 이본으로 평가할 수 있다. 적어도 박효관 주변에서 필사된 책이며, 박효관이 직접 엮고 필사한 책일 가능성도 높다. 편찬 시기가 늦어도 1853년이므로, 『가곡원류』와 같은 가집의 체재가 이미 19세기 전반기에 완성되었음을 알 수 있다. 또한 『여창가요록』은 이후의 집대성 완성본 『가곡원류』에서 별도로 파생된 것이 아니라 오히려 먼저 이루어졌다. 여창 중심의 기방 유행본 『가곡원류』가 이미 초기본의 형태로 존재했다. 이후 안민영과의 공동 작업으로 국악원본 『가곡원류』와 같은 증보 완성본이 편찬된 것으로 파악할 수 있다. 양승민본은 『가곡원류』의 형성 시기가 사실상 19세기 전반기임을 보여준다. 이 초기본의 발견으로 『가곡원류』의 형성기 모습 자체에 대한 연구가 구체적으로 가능해졌다. 『가 곡원류』라는 가집은 일시에 완성된 것이 아니라 19세기 가단의 움직임 속에서 다양하고 점진적인 형성 과정이 있었고, 여러 형태의 이본들이 당대의 시대적, 상황적 수요에 따라 유통되었다. This paper presents an introduction and examination of "Yeochanggayorok" of the Yang Seung-min version that had been newly excavated. The Yang Seung-min version of "Yeochanggayorok" was an anthology transcribed and provided by Master Woongok to a Gyeonggi gisaeng called Maiden Hakseon in 1853. It is an early version that is the oldest of the different remaining versions of "Yeochanggayorok." Since it was published before 1853 at the latest, it offers important materials to show the early formation process of "Gagokwonryu." First, the Yang Seung-min version of "Yeochanggayorok" is widely equipped with the formal framework of "Gagokwonryu" and accordingly another version of its in spite of the fact that it was published with a focus on Yeochang. Second, it is estimated that Park Hyo-gwan himself compiled and transcribed the Yang Seung-min version and published it in 1853. Such Yeochang-centric anthologies as the version must have been published before the completion of "Gagokwonryu" and in vogue around gisaengs`` saloons. Third, the version claims its worth as a material to advance the formation time of "Gagokwonryu" to the former half of the 19th century at the latest. The discovery of this early version of "Yeochanggayorok" made it possible to conduct concrete research on the aspects of "Gagokwonryu" themselves during its formation period. Finally, it is estimated that the anthology of "Gagokwonryu" was not completed at a time but went through various gradual formation processes in the middle of the movements of Gadan in the 19th century. As a matter of fact, there was no certain complete version of "Gagokwonryu"; instead, its versions of many different forms were widely distributed according to the demands of the times and situations those days.

The role of CD14 and Toll-like receptors on the release of MMP-B in the LPS recognition pathway

양승민,김태일,설양조,이용무,구영,정종평,한수부,류인철,Yang, Seung-Min,Kim, Tae-li,Seol, Yang-Jo,Lee, Yang-Moo,Ku, Young,Chung, Chong-Pyoung,Han, Soo-Boo,Rhyu, In-Chul The Korean Academy of Periodontoloy 2006 Journal of Periodontal & Implant Science Vol.36 No.3

1. 연구배경 교원질 분해작용을 하는 호중구의 세포질 효소인 기질금속단백분해효소-8은 치주질환, 류마티스 관절염, 그리고 궤양결장염과 같은 염증성 질환에서 농도가 증가한다고 알려져 있다. 최근에는 A. actinomycetemcomitans의 leukotoxin이 사람호중구에서 기질금속단백분해효소-8의 분비를 유도하는 것이 보고되었다. 이 연구의 목적은 선천면역 체계에서 세포표면 항원무리14, Toll-like 수용기, 그리고 $NF-{\kappa}$ B경로를 통하여 A. actinomycetemcomitans의 지질다당질로 유도된 기질금속단백분해효소-8의 분비 여부와 세포기전을 알아보고자 하였다. 2. 연구재료 및 방법 건강한 개인 제공자(남자 13명, 여자 3명)로부터 얻은 개개인의 20ml 말초혈액을 제조사의 지침에 따라 호중구를 추출한 후 항세포표면 항원무리14와 함께 $4^{\circ}C$에서 30분간 전배양 한 후, $37^{\circ}C$에서 9시간 동안 배양시켰다. 추출한 호중구에 Toll-like 수용기 억제제 또는 $NF-{\kappa}$ B억제제인 TPCK를 첨가한 후 $37^{\circ}C$에서 1시간 동안 전배양하고 $37^{\circ}C$에서 9시간 동안 배양시켰다. 호중구에 세포뼈대 억제제인 cholchicine, nocodazole, demecolcine, 그리고 cytochalasin B를 A. actinomycetemcomitans의 지질다당질과 함께 $37^{\circ}C$에서 9시간 동안 배양시켰다. 기질금속단백분해효소-8 분비량은 효소면역측정법을 통해 결정하였다. 통계처리는 일원배치 분산분석법을 이용하였다(p<0.05). 3. 결과 A. actinomycetemcomitans 지질다당질은 기질금속단백분해효소-8의 분비를 증가시켰다. 기질금속단백분해효소-8의 분비는 항세포표면 항원무리14에 의해서 억제되었지만, 항 Toll-like 수용기2, 항 Toll-like 수용기4 항체는 억제시키지 못했다. $NF-{\kappa}$ B 억제제는 A. actinomycetemcomitans의 지질다당질로 유도된 $NF-{\kappa}$ B 결합 활성도와 기질금속단백분해효소-8 분비를 억제하였다. 미세섬유 중합반응 억제제는 A. actinomycetemcomitans의 지질다당질로 유도된 기질금속단백분해효소-8의 분비를 억제시켰으나, 미세관 중합반응억제제는 억제시키지 못했다. 4. 결론 위의 연구결과를 종합하여 볼 때, 기질금속단백분해효소-8은 A. actinomycetemcomitans의 지질다당질로 유도되며, 세포표면 항원무리-$NF-{\kappa}$ B 경로를 통하여 분비되고, 이 분비 과정은 미세섬유 계통이 관여하는 것으로 보인다.

다양한 형태의 다공질 Calcium Polyphosphate의 생분해성에 관한 장기적인 연구

양승민,설양조,계승범,이인경,이철우,김석영,이용무,구영,한수부,정종평,최상묵,류인철,Yang, S.M.,Seol, Y.J.,Kye, S.B.,Lee, I.K.,Lee, C.W.,Kim, S.Y.,Lee, Yong-Mu,Ku, Y.,Han, S.B.,Chung, C.P.,Choi, S.M.,Rhyu, I.C. 대한치주과학회 2003 Journal of Periodontal & Implant Science Vol.33 No.2

The purpose of this study is to evaluate the biocompatibility and the biorsorbability of several types of calcium polyphosphate made through change of manufacturing process for 12 month. To solve limitation of calcium phosphate, we developed a new ceramic, Calcium Polyphosphate(CPP), and report the biologic response to CPP in extraction sites of beagle dog. Porous CPP blocks were prepared by condensation of anhydrous $Ca(H_2PO_4)_2$ to form non-crystalline $Ca(PO_3)_2$ and then milled to produce CPP powder. CPP powder, CPP block, and CPP granules added with $Na_2O$ were implanted in extraction sockets and histologic observation were performed at 12 months later. Like 3 months results, histologic observation at 12 months revealed that CPP matrix were mingled with and directly apposed to new bone without any adverse tissue reaction, CPP powder show direct bony contact, but new bone formation and fibrous tissue encapsulation showed in CPP block. 10% $Na_2O$ CPP granules show more inflammatory cells infiltration around graft materials compared at 3 month, but 15% $Na_2O$ CPP granules show less. This result revealed that regardless of addition of $Na_2O$, CPP had a high affinity for bone and had been resorbed slowly. From this results, it was suggested that CPP is promising ceramic as a bone substitute and addition of $Na_2O$ help biodegradation but optimal concentration of $Na_2O$ and other additive component to increase degradation rate should be determined in further study.

골이식재로서의 Calcium Polyphosphate 평가;동물 연구

양승민,이승진,김석영,임윤탁,계승범,이인경,이용무,한수부,정종평,류인철,Yang, S.M.,Lee, S.J.,Kim, S.Y.,Lim, Y.T.,Kye, S.B.,Lee, I.K.,Lee, Y.M.,Han, S.B.,Chung, J.P.,Rhyu, I.C. 대한치주과학회 2003 Journal of Periodontal & Implant Science Vol.33 No.2

치주조직재생을 도모하기 위한 전통적인 시술방법으로는 여러 가지 골이식재를 이용한 골이식술이 오래 전부터 이용되고 있다. 이번 실험의 목적은 장,단기간의 신생골의 형성과 CPP의 흡수를 비교하여 골이식재로서 Calcium Polyphosphate(CPP)를 평가하는 것이다. 이번 실험에 사용된 CPP는 무수 $Ca(H_2PO_4)$를 condensation하여 무결정의 $Ca(PO_3)_2$를 얻고 이를 용융하고 냉각시킨후 분쇄하여 얻은 것으로 키토산이나 $Na_2O$를 첨가한 후 3세된 비글견에 이식하여 관찰하였다. 양성 대조군으로 동결탈회건조골을 이용하였다. 조직학적으로 3개월 소견과 같이 섬유조직의 게재없이 신생골의 형성이 관찰되었다. 12개월 후의 신생골의 형성은 3개월 결과에 비해서 동결탈회건조골이나 키토산, $Na_2O$를 넣은 CPP 과립에서 더 많은 비율로 나타났다. 음성 대조군과 이식재를 넣은 군간에는 유의성이 있는 것으로 나타났고(p<0.05), 또한 키토산을 첨가한 CPP 과립과 $Na_2O$를 첨가한 CPP 과립 사이에는 신생골의 형성에 유의성이 없었다. 이식한 CPP 과립의 경우 흡수소견이 3개월 결과에 비해 크게 증가하지 않았다. 이번 실험에서는 첨가물에 상관없이 CPP 과립은 골유도성과 생체적합성을 보였다. 그러나 흡수속도가 매우 느려 신생골로 대체되는 여부를 알 수 없었다. 향후 연구에서는 흡수속도를 증가시킬 수 있는 적당한 CPP 형태와 첨가물을 밝혀내어야 할 것이다.

과다알부민 생산증 - PCR 을 이용한 유전병 진단

양승민,박선희,김승현,박종상,문형로 ( S . M . Yang,S . H . Park,S . H . Kim,J . S . Park,H . R . Moon ) 생화학분자생물학회 1992 BMB Reports Vol.25 No.1

From the hyperalbuminemia patient who had overexpressed albumin, his genomic DNA was isolated and its albumin gene promoter region was amplified by using PCR and subcloned into pTZ 19U vector. The longer the extension time was the more product was yielded. Specificity was increased when higher annealing temperature and less concentration of two primers were used. Based on RNA slot blotting the patient`s albumin gene should be expressed ten-fold higher than that of the normal. We sequenced 600 base pair of human albumin promotor region and did not find any mutation. Thus the hyperalbuminemia might result from the mutation on the transcription factors of albumin promoter.

IT대학 특성화를 위한 제언

양승민,Yang, Seung-Min 한국대학교육협의회 2008 大學敎育 Vol.152 No.-

치근단 수출 시 형성된 골결손 부위를 가진 치아의 발치 후 즉시 식립 임플란트에서 골 접촉률에 대한 흡수성 차폐막의 효과

양승민,계승범,신승윤,Yang, Seung-Min,Kye, Seung-Beom,Shin, Seung-Yun 대한치주과학회 2008 Journal of Periodontal & Implant Science Vol.38 No.4

Purpose: The guided bone regeneration (GBR) technique is widely used in periradicular surgery. However, there is still some controversy regarding the effectiveness of GBR in promoting bone healing after periradicular surgery. The purpose of this study was to evaluate the resorbable membrane on the osteointegration of immediate implants in sites with periradicular lesion that had been removed by periradicular surgery. Materials and methods: Six roots of lower second premolars and 15 roots of lower third and fourth premolars of dogs were used as control and experimental teeth, respectively. Periradicular lesions were induced only in the experimental teeth. Twelve weeks later, the control and experimental teeth were extracted and implants were placed immediately. Periradicular lesions were removed with osteotomy, curettage and saline irrigation. Resorbable membranes were used in experimental group 1 but not in experimental group 2. After 12 week of healing period, the implants were clinically not mobile and showed no signs of infection. Data obtained by histomorphometric analysis were analyzed by Kruskal-Wallis test. Results: The control group showed a significantly higher bone to implant contact (BIC) ($74.14{\pm}16.18$) than experimental group 1 ($40.28{\pm}15.96$) and 2 ($48.70{\pm}17.75$)(p<0.05). However, there was no significant difference between experimental group 1 and 2. Conclusion: Although BIC in experimental groups were lower than in control group, immediate implant can be successfully placed at extraction socket with periradicular lesion and osseous defect. However, the use of resorbable membrane in bony defect created during periradicular surgery was questioned.

본 히펠 린다우병에서 발생한 신장암의 뇌전이 - 증례보고 -

양승민,박상우,석종식,박종혁,권정택,민병국,Yang, Seung Min,Park, Sang Woo,Suk, Jong Sik,Park, Jong Hyuk,Kwon, Jeong Taik,Min, Byung Kook 대한신경외과학회 2001 Journal of Korean neurosurgical society Vol.30 No.8

Although brain metastasis of renal cell carcinoma is a major cause of death in Von Hippel-Lindau disease(VHL), it is not easy to distinguish local recurrence of hemangioblastoma from distant metastasis. In addition, hemangioblastoma has several characteristics suitable for recipient of "tumor-to-tumor metastasis". Authors report a case of Von Hippel-Lindau disease that had metastases of renal cell carcinomas.

E. coli lipopolysaccharides로 유도된 사람 호중구에서 CD14, Toll-like receptors, cytoskeletal inhibitors 그리고 $NF-{\kappa}B$ inhibitor가 MMP-8 분비에 미치는 영향

양승민,김태일,설양조,이용무,구영,정종평,한수부,류인철,Yang, Seung-Min,Kim, Tae-Il,Seol, Yang-Jo,Lee, Yong-Moo,Ku, Young,Chung, Chong-Pyoung,Han, Soo-Boo,Rhyu, In-Chul 대한치주과학회 2005 Journal of Periodontal & Implant Science Vol.35 No.2

Objective: MMP-8 is a neutrophil enzyme and its level increases in some inflammatory diseases, including periodontal disease. We knew that the lipopolysaccharide of E.coli(E-LPS) induced MMP-8 release from human neutrophils. E-LPS is known to induce the production and release of inflammatory cytokines through CD14, Toll-like receptor(TLR). In the present study, we investigated whether MMP-8 release by E-LPS is induced via CD14-TLR pathway and the cellular mechanism of MMP-8 release in human neutrophils. Material and methods: Human neutrophils were isolated from the peripheral blood of healthy donors and pre-incubated in medium containing antibodies against CD14, anti-TLR2 and anti-TLR4 or several inhibitors of microtubules and microfilaments and then incubated with E-LPS. The cells were treated TPCK and E-LPS simultaneously. The MMP-8amount in the culture medium was determined using ELISA. Results: E-LPS increased MMP-8release from neutrophils and its induction was inhibited by anti-CD14 and anti-TLR4 but not by anti-TLR2 antibodies. The inhibitors of microtubule and microfilament polymerization significantly decreased E-LPS-induced MMP-8release. TPCK inhibited E-LPS-induced MMP-8 release. Conclusion: These results suggest that MMP-8 release is induced by E-LPS via the CD14-TLR4 signal pathway in human neutrophils and may be depedent on microtubule and microfilament systems and $NF-{\kappa}B$ pathway.

다공질 Calcium Polyphosphate의 생분해성에 관한 연구

양승민,이영규,한은영,김석영,계승법,이승진,이용무,구영,한수부,정종평,최상묵,류인철,Yang, Seung-Min,Lee, Young-Kyoo,Han, Eun-Young,Kim, Seok-Young,Kye, Seung-Beom,Lee, Seung-Jin,Lee, Yong-Moo,Ku, Young,Han, Soo-Boo,Chung, Chong-Pyoung,Ch 대한치주과학회 2001 Journal of Periodontal & Implant Science Vol.31 No.3

The purpose of this study is to evaluate the bioresorbability of Calcium Polyphosphate added with $Na_2O$ and chitosan. Though calcium phosphate ceramics meet some of the needs for bone replacement, they have some limitation of unresorbability and fibrous encapsulation without direct bone apposition during bone remodelling. To solve these problem, we developed a new ceramic, calcium polyphosphate(CPP), and report the biologic response to CPP in extraction sites of beagle dog. Porous CPP granules were prepared by condensation of anhydrous $Ca(H_2PO_4)_2$ to form non-crystalline $Ca(PO_3)_2$. CPP granules added with $Na_2O$ and chitosan were implanted in extraction sockets and histologic observation were performed at 12 weeks later. Histologic observation at 12 weeks revealed that CPP matrix were mingled with and directly apposed to new bone without any intervention of fibrous connective tissue. CPP granules added with chitosan were well adatped without any adverse tissue reaction and resorbed slowly and spontaneously. CPP granules added with $Na_2O$ and chitosan show multinucleated giant cells and osteoblast-like cells around grafted material and newly formed bone. This result revealed that CPP, regardless of its additive component, had a high affinity for bone and had been resorbed slowly. From this results, it was suggested that CPP is promising ceramic as a bone substitute and addition of $Na_2O$ and chitosan help biodegradation. In further study , it will be determined which concentration of $Na_2O$ help biodegradation and the other additive components increase the degradation rate.