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Moutan radix 의 mouse sarcoma 180 cell 로 유발한 mouse ascites cancer 에 대한 항암효과
반경녀(Kyeong N . Bahn),이은주(Eun J . Lee),양민석(Min S . Yang),김정옥(Jeong O . Kim),하영래(Yeong L . Ha) 한국응용생명화학회 1995 Applied Biological Chemistry (Appl Biol Chem) Vol.38 No.4
Anticarcinogenic activity of Moutan radix for mouse ascites cancer induced by mouse Sarcoma 180 (S-180) cells was investigated. Methanol extract of Moutan radix including other folk medicinal plants (Taxus cuspidata, Curcuma Tonga, Artemisia capillaris, Ligrstri fructus, and Liriope platyphylla) used to remedy or cure many chronic human diseases like cancer was fractionated into hexane, chloroform (CHCl₃), ethylacetate (EtOAc), and butanol (BuOH) fractions. Anticarcinogenic activity of the fractions, exhibited a strong cytotoxicity for L1210 and S-180 cells, was examined for mouse ascites cancer induced by S-180 cells. Male ICR mice (7 mice/treatment, 5∼6 weeks of age, 23±1 g) were injected i.p. with S-180 cells (1×10^7 cell/1 ㎖ PBS). One day later, each mouse was given 0.1 ㎖ of 10% DMSO containing sample (30 ㎍/g body weight) every day for 10 consecutive days. Control mice were only given 0.1 ㎖ S-180 cells and 0.1 ㎖ 10% DMSO. Mice treated with EtOAc fraction of Moutan radix showed 28.7 days of life, which is 167% of control mice`s life. Based on the dose-dependant experiment, mice treated with 30 ㎍ showed longer life relative to mice treated with other doses (5, 15, 60 ㎍) and mice treated with 60 ㎍ exhibited toxic symptoms. Body weight of mice treated with Moutan radix was significantly reduced relative to that of control mice (p$lt;0.05). GC-MS analysis in conjunction with silica-gel column chromatography revealed that the EtOAc fraction contained 2-methoxylphenol, benzoic acid, 1-(4-hydroxy-3-methoxyphenyl)ethanone, 8-methyl-2,4(1H,3H)pteridinedione and 2,5-furan-dicarboxylic dimethyl ester as regards to the anticarcinogenic property of the EtOAc fraction. These results suggest that Moutan radix might be included as an anticarcinogenic medicinal plant for treatment of ascites cancer.
박철우,반경녀,이영남,김정옥,김민석,하영래,Park, Cherl-Woo,Bahn, Kyeong-Nyeo,Lee, Young-Nam,Kim, Jeong-Ok,Kim, Min-Seok,Ha, Yeong-Lae 한국식품영양과학회 2007 한국식품영양과학회지 Vol.36 No.3
The concentrations of conjugated linoleic acid (CLA) in twelve Korean mothers' milk samples collected in October 2006 and four infant formula samples produced by Korean manufacturers were determined. CLA detected from both mothers' milk and infant formula samples was only cis-9, trans-11 CLA isomer. The amount of cis-9, trans-11 CLA in Korean mothers' milk (100 mL) ranged from 4.32 to 10.12 mg ($6.27{\pm}0.28\;mg$). Infant formula (13 g), which is the manufacturers' recommended amount to make up 100 mL with water, contained cis-9, trans-11 CLA isomer in amounts ranging from 1.61 to 2.97 mg ($2.27{\pm}0.31\;mg$). 한국인 모유에는 cis-9, trans-11 CLA 이성체만 동정되었고, 그 함량은 $4.32{\sim}10.12\;mg/100\;mL$(평균 $6.27{\pm}0.28\;mg/100\;mL$)이었고, 지방함량 중에는 $1.35{\sim}3.13\;mg/g$(평균 $1.96{\pm}0.06\;mg/g$)으로 개인에 따라 큰 차이가 있었다. 한국인 모유에 함유된 CLA의 함량은 외국의 CLA 함량에 비해 낮았고, 그 이유는 식사의 차이 때문인 것으로 추정되었다. 국내에서 제조된 조제분유에서도 cis-9, trans-11 CLA만 동정되었는데, 그 함량은 $2.35{\sim}22.82\;mg/100\;g$으로 평균치는 $17.45{\pm}1.24\;mg/100\;g$이었고, 지방에 함유된 cis-9, trans-11 CLA 함량은 $0.45\;mg/g{\sim}0.85\;mg/g$으로 그 평균치는 $0.65{\pm}0.02\;mg/g$이었다. 영유아가 조제분유 제조사의 지시된 13% 조제분유를 섭취할 경우 cis-9, trans-11 CLA를 2.27 mg 섭취하는 것으로 모유 100 mL에 함유되어 있는 양에 비해 낮다. 따라서 조제분유를 조제할 때 모유와 동일한 cis-9, trans-11 CLA를 섭취할 수 있도록 조제분유를 제조하는 것이 바람직하다.
히드록시프로필인산이전분의 제조조건에 따른 부산물 생성에 과한 연구
조태용,이명자,반경녀,오세동,홍기형,김선봉,최태진,홍무기 식품의약품안전청 2001 식품의약품안전청 연보 Vol.5 No.-
히드록시프로필인산이전분의 안전한 사용과 품질관리를 위하여 propylene chlorohydrin(PCH)의 분석법을 개선하고 생성 정도를 제조조건별로 조사하였다. PCH의 가속용매추출장치(린르도)에 의한 최적 분석조건을 확립하꾸 위하여 추출용매4초산에틸, 메탄올, 아세톤, 헥산 및 에테르), 추출압력(1000~린Oopsi) 및 추출온도(40'E~150'E)를 변화시켜 PCH의 회수율을 검토한 갈과 초산에틸을 추출똥매로 산웅하여 추출압력 2300psi 및 추출온도 120'c로 한였을 때 회수율은 뽄.1 %를 나타내어 PCH 최적 분석 조건으로 하였다. 히드록시프로필인산이전분의 제조조건에 따른 PCH 잔류량을 조사하기 위하여 찰옥수수전분과 옥수 순전분을 사용하여 Propylene oxidefppo) 첨가량(2, 5, 7 및 10%)에 따른 변성정도 및 PC닌함량의 변화들을 조사하였을 때 PPO 첨가량이 증가함에 따라 PC러의 잔류량과 nS(Degree of substituent)가 증가하였는데 찰옥수수전분을 사용한 경우가 옥수수전분에 비해 DS도 높았고 PC보 잔류량도 적었다.히드록시프로필화(fP) 및 가교화fXL)반응의 순저에 따른 DS 및 PCH 잔류량을 조사한 결과 DS는 큰 차이를 나타내지 않았으나 X교-HP의 경우가 PCH 잔류량이 다소 많은 경향을 보였다. 중쑤시약으로 염산, 황산 및 인산을 각각 사용하였글 때 PC또 잔류량은 염산을 사용하였을 경우 가장 높았으나 황산과 인산을 자용한 경우에돈 세척하지 않은 것에서 3.60ppm 및 4.07ppm이 각각 검출되었다. 제조과정 중 잔류하는 PPO을 제거하기 위하여 HP(PPO 10% 첨가)화한 후 은도 45'c를 유지하면서 반응조 뚜껑을 열어 PPO를 휘발시킴과 동시에 완전한 제거를 위하여 질소가스를 200myftn으로 30분간 흘린 후 제조하였을 때 제척하지 않은 경우에도 PCH 잔류량이 Ippm 이하였고 세척한 경우에는 완전히 제거되었다. This study was carried out to improve the analytical method of propylene chlorohydrin(PCH) and to examine the PCH residue by various reaction conditions in the preparation of hydroxypropyl distarch phosphate. By use of ASE(Accelerated Solvent Extractor), the effect of temperature(40∼150℃), pressure(1000∼2500psi) and solvent(ethyl acetate, methanol, aceton, hexane, ether) were investigated to find the optimum conditions which was at 120℃, 2500psi with ethyl acetate. The recovery was above 95% We also examined the PCH residues during the preparation of hydroxypropyl derivatives of waxy corn and corn strch. Degree of substituent(DS) and PCH content were increased with propylene oxide(PPO) level. Modified waxy corn starch had higher DS, lower PCH content than modified corn starch. Effect of DS was no difference between hydroxypropylation(HP) and crosslinking(XL) modification sequence, but the formation of PCH was more affective by XL-HP. When hydrochloric acid as neutralizing reagent was added, PCH residue was the highest. Even though using sulfuric acid and phoshoric acid which have no chloride ion, PCH content each reached to 3.60ppm and 4.07ppm in no washed corn starch. To remove PPO residue after hydroxypropylation, at the same time the reaction temperature(45℃) was maintained, the reaction vessel cover was opened and incorproated nitrogen into starch slurry at the rate of 200ml/min for 30min. In that results, PCH residue was reduced to less than 1ppm which was acceptable, based on Code of Food Additives codex, in no washed modified starches, and after being washed, it was completely removed.
임호수,김소희,반경녀,김준현,장귀현,문귀임,양효진,박성관,박혜경 한국식품위생안전성학회 2010 한국식품위생안전성학회지 Vol.25 No.2
This study was conducted to establish a method to analyze azodicarbonamide (ADA) in wheat flour. A new method using high performance liquid chromatography (HPLC) was developed for the determination of ADA in wheat flour. The recovery rate was 91.93~97.54%. The limit of detection for ADA was 0.02 mg/kg and the limit of quantification was 0.05 mg/kg. The monitoring results for ADA contents using the established methods showed that it was detected as the low value of 0.95 mg/kg in one of 51 flour samples (detection rate : 2%), but not detected in 59 bakery samples. The detected ADA level was suitable to its usage standard, compared to the standard (45 mg/kg). Although the detection rate was very low, the established analytical method of ADA will contribute to the management of ADA in processed foods such as wheat flour and bakery.