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구강내 점막과 유리피판에 사용되는 피부의 rete ridge에 관한 2차원 및 3차원적 구조 연구
안강민(Kang-Min Ahn),정헌종(Hun-Jong Chung),김윤태(Yoon-Tae Kim),팽준영(Jun-Young Paeng),신영민(Young-Min Shin),성미애(Mi-Ae Sung),박희정(Hee-Jung Park),명훈(Hoon Myoung),황순정(Soon-Jung Hwang),최진영(Jin-Young Choi),정필훈(Pill-Ho 대한구강악안면외과학회 2005 대한구강악안면외과학회지 Vol.31 No.2
Objects : With the advancement of tissue engineering techniques, the effort to develop bioartificial mucosa have been actively delivered. The problem we met with this technique is the lack of mechanical strength between kerationocyte layer and dermal layer, where in the normal skin and mucosa, they are tightly bound with rete ridge structure. The purpose of this study is to understand the 2D and 3D structure of rete ridge of mucosa and skin paddle for rendering more biomimetic structure to the artificial mucosa. Materials and Methods : Oral mucosa and skin from the patients who received the oral surgery and maxillofacial reconstruction were harvested. The epidermis was separated from the dermis after treating with dispase for 12-16 hours. H & E staining was performed for 2D(dimensional) structure study and confocal LASER and SEM study were performed for 3D structure. Mean height(Sc) and arithmetic mean deviation(Sa) of all surface height were calculated. Results : The average height of rete ridge of skin flap was between 67.14㎛ and 194.55㎛. That of oral mucosa was between 146.26㎛ and 167.51㎛. Pressure bearing area and attached gingiva of oral mucosa showed deeper rete ridges. Conclusion : To obtain the adequate strength of artificially cultured keratinocyte skin and mucosa flap, it is necessary to imitate the original skin and mucosa structure, especially rete ridge. Through this study, 2D and 3D rete ridge structure of normal mucosa and skin was obtained. These results can be used as basis for substrate morphology for keratinocytes culture.
가토모델에서 배양 구강상피를 이용한 근-점막 피판의 형성에 관한 연구
신영민,정헌종,안강민,박희정,성미애,김성민,황순정,김명진,장정원,김성포,양은경,송계영,이종호,Shin, Young-Min,Chung, Hun-Jong,Ahn, Kang-Min,Park, Hee-Jung,Sung, Mi-Ae,Kim, Soung-Min,Hwang, Soon-Jung,Kim, Myung-Jin,Jahng, Jeong-Won,Kim, Sung-P 대한악안면성형재건외과학회 2005 Maxillofacial Plastic Reconstructive Surgery Vol.27 No.3
Purpose : Extensive defect of oral and maxillofacial area is usually reconstructed with composite flap including skin paddle. However, if the defects are lined with only skin components, the mucosa's role in mastication and texture are not restored. Furthermore, stiffness and hair-growing prevent denture rehabilitation and good oral hygiene. This study was performed to overcome the disadvantages of composite soft tissue flaps including the skin and to make a model for myo-mucosal flaps. Materials and methods : Buccal mucosa sized $0.5\times1.0\;cm^2$ from New Zealand rabbit (around 1.5kg) was harvested and cultivated by the modification of Rheinwald and Green's keratinocyte culture method. Cultured mucosa was grafted on the fascia of latismus dorsi as form of mucosal sheet. After 7, 10, 14 days, the myomucosal flap was excised and evaluated under light microscope with H & E and immunohistochemical staining. As control group, harvested buccal mucosa from rabbit was transplanted to gracilis muscle(n=6). Results : From 7 days after prelamination, the basal layer of the grafted mucosa resembled that of normal mucosa. As control group, transplanted mucosa had original shape but there's slight inflammatory reaction. Prelaminated mucosa has 19.8$\pm$4.59 cell layers and some samples have more than 20 layers. The expression rate of PCNA was relatively strong (42.9%$\pm$14.1) at the basal layer of grafted mucosa and the laminin was found at the basal layer. On the contrary, prelaminated mucosa at 10 days showed moderate expression rate of PCNA(32.4%$\pm$4.62). We found the mucosal layer was somehow disappeared and there is strong inflammatory reaction. After 14 days prelamination, the grafted oral keratinocytes were almost disappeared and expression of PCNA was not observed. Conclusion : We can make 75 fold large mucosal($3850mm^2$) sheet from small samples of mucosa $(50mm^2)$. Epithelial sheet that grafted on the fascia of muscle underwent differentiation and proliferation. But after 10, 14 days, there was strong inflammatory reaction and the grafted mucosa was destroyed from surface layer. In rabbit model, transfer of fascio-mucosal flap should be done from 7 to 10 days after prelamination.
우리나라 청소년의 구취증상 경험과 스트레스 및 심리상태와의 관계
박희정(Park, Hee-Jung),한성민(Han, Seong Min) 한국산학기술학회 2016 한국산학기술학회논문지 Vol.17 No.1
본 연구는 우리나라 청소년이 경험하는 구취증상과 스트레스 및 심리상태간의 구조적 관계를 검증하는데 목적을 두었다. 이를 위해 2010 한국청소년건강실태조사 원자료를 활용하였고 연구대상자는 중․고등학생 3,689명(남학생 1,805명, 여학생 1,884명)이었다. 단변량 분석에서 확인된 구취와 관련된 요인은 경로모형에서 통제변수로 설정하여 투입하였고, 구취 증상과 스트레스, 그리고 우울감, 불안, 긴장, 적대감으로 측정된 심리상태와의 관계와 그 경로를 검증하였다. 분석결과, 구취 증상 경험이 있는 청소년은 27.4% 이었고, 경로모형을 검증한 결과에서는 구취증상→스트레스(β=0.083, P<0.001), 구취증상 →우울(β=0.067, P<0.001), 구취증상→불안(β=0.072, P<0.001), 구취증상→긴장(β=0.072, P<0.001), 구취증상→적대감(β =01.074, P<0.001)로 가는 경로가 유의하게 나타났다. 또한 구취증상은 스트레스를 매개로 우울, 불안, 긴장, 적대감에 부정적 인 영향을 미치는 간접효과를 확인할 수 있었다. 결론적으로 구취를 예방하는데 있어 스트레스와 다양한 심리적인 요소도 고려되어야 할 것이며 구취가 있는 청소년에게서 심리적 지지를 위한 지원과 강화방안이 논의되어야 할 것이다. This study examined the relationships among halitosis, stress and psychological status using a cross-sectional survey of 3,689 adolescents (1,805 male 1,884 female) from the 2010 Korean Survey on the Health of Youth and Children. The related factors of halitosis were checked and the path model was also examined using AMOS 19.0 programs. The prevalence of halitosis was 27.4%. Based on path analysis, the significant coefficients paths were as follows: halitosis→stress, halitosis→depression, halitosis→anxiety, halitosis→tension, halitosis→hostility. Halitosis had an indirect effect on the psychological status linked to depression, anxiety, tension, and hostility via stress. Overall, the results provide stronger support for the halitosis impact of stress and psychological health. Moreover, the effective prevention of halitosis may be important for understanding the specific psychological factors.
박희정(Hee-Jung Park),신혜정(Hye-Jung Shin),박성종(Seong-Jong Park),정년기(Yeon-Gi Jeong),박은정(Eun-Jeong Park),허수민(Su-Min Heo),정옥란(Ok-Ran Jeong),석동일(Dong-Il Seok) 한국언어치료학회 2006 言語治療硏究 Vol.15 No.4
The purpose of this study was designed for the specific purpose of identifying and quantifying preserved abilities that might form the beginning steps of rehabilitation programs for severely aphasic patients. The Korean version of BASA was developed. The stimulus materials and tasks were to develop and to modify on the basis of Boston Assessment of Severe Aphasia(BASA). The K-BASA provided information that these patients may retain the ability to process certain of linguistic and paralinguistic information seldom probed in order aphasic measures. This instrument was 61 items, these were grouped according to task requirements and item content in terms of auditory comprehension, praxia, oral-gestural expression, reading comprehension, gesture recognition, writing, and visuo-spatial skills. Accordingly, the K-BASA may be used to design an individual treatment program the encourages further development of the patients strongest response modality, using stimuli that elicit the most communicative response.
구강건조증이 구강건강관련 삶의 질에 미치는 관계에서 심리적 요인의 매개효과연구
박희정 ( Hee Jung Park ),손민성 ( Min Sung Sohn ),이준협 ( Jun Hyup Lee ),진보형 ( Bo Hyoung Jin ),이하영 ( Sop Hia Lee ),김태일 ( Tae Il Kim ) 대한구강보건학회 2014 大韓口腔保健學會誌 Vol.38 No.1
Objectives: The aim of this study was to investigate the role of psychological factors as mediators in the casual relationship between mouth dryness and oral health-related quality of life of adults and to explore gender differences in what psychological factors play an important role in mediating the path of mouth dryness to quality of life. Methods: Self-administered questionnaires were distributed to 321 adults who are above 20 and under65 years of age. Collected data were analyzed via statistical packages using a multi-group analysis through the SEM (Structural Equation Model). Results: Based on the statistical analysis, stress was a major psychological factor in male subjects. While depression served as a main mediator in female subjects. We discovered that the path coefficients of the following paths showed significant gender differences: mouth dryness → depression, depression→ quality of life, stress → quality of life. Conclusions: A gender-specific approach regarding effective prevention of stress and depression maybe important for understanding the relationship between mouth dryness and quality of life.
박희정(Hee-Jeoung Park),박재혁(Jae-Hyeok Park),김규민(Gyu-Min Kim),이재응(Jae-Eung Lee) 대한기계학회 2017 대한기계학회 춘추학술대회 Vol.2017 No.11
The multi-function dot printer is printing equipment that wires of a head strike an ink ribbon on a paper. The dot printer always produces noise problems. The printer`s main source of noise is the head, carriage motor, and platen, which are located adjacent to the printer`s small interior space. Therefore, in the measurement of each noise source, there are difficulties due to narrow space and adjacent noise sources. In this paper, we analyze the contribution of the noise generated by the each noise source through the spectrum measurement and propose the noise reduction method based on these analysis.