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행인 과루인 추출물이 마우스 대식세포주인 RAW264.7 세포주의 iNOS 발현 및 Superoxide 형성에 미치는 영향
박정운,문석재,문구,원진희 대한한방종양학회 1999 대한한방종양학회지 Vol.5 No.1
Macrophage play a major role in host defence against infection and tumor development and this activity is regulated through the production of several mediators. In particular, the production of N0 by macrophages mediates killing or growth inhibition of tumor cells, bacteria, fungi and parasites. However, over-expression of iNOS by various stimuli, resulting in over-production of NO. contributes to the pathogenesis of septic shock and some infmammator and auto-immune disease. Thereforc, it would be valuable to develop potent and selective inhibitors of for potential therapeutic use. Thus the agent that supprees the expression of iNOS mRNA or enzyme protein will be usefull for the prevention of various diseases. We are intersted in identifying selective inhibitiors of iN0S which might be useful intreating inlammatory human diseases. In summary, we have demantrated that scopolein, isolated from Seman Armenicae and Radix Trichosalmis the production of NO induced by IFNy plus LPS in RAW 264.7 macrophages, The mechanism for the inhibition of N0 Production was due to suppression of the expression of iN0S mRNA or enzyme protein.
새로운 저온 내성세균의 동정과 $\beta-Galactosidase$ 생산
박정운,유재수,노동현,Park, Jeong-Woon,Yoo, Jae-Soo,Roh, Dong-Hyun 한국미생물학회 2006 미생물학회지 Vol.42 No.1
우유에 풍부하게 존재하는 유당은 galactose와 포도당의 $\beta(1\rightarrow4)$ glycosidic 결합으로 구성되어 있고, 인간에서 이를 가수분해하는 효소는 lactase, 세균에서는 $\beta-galactosidase$로 알려져 있다. Lactase의 활성이 낮은 사람이 우유를 섭취했을 경우 일시적인 설사를 일으키고 때로는 만성적인 대감의 염증으로 인한 만성설사의 원인이 되기도 한다. 겨울철에 젖소를 사육하는 축사 주변에서 저온에서 생육하는 세균 AS-20을 분리하여 $\beta-galactosidase$ 활성을 갖는 균주를 선별하고 온도별로 분리균의 성장을 조사하였다. 그 결과 대장균이 자라지 못하는 $10^{\circ}C$에서도 분리된 AS-20은 생육이 가능하였고 생육 최적온도는 $30^{\circ}C$이였으며 이 온도에서 세대시간은 60여분 이었다. AS-20의 생화학적 특성을 bioMerieux Vitek Gram negative identification card (GNI+)로 조사한 결과 포도당을 발효, 산화시켰으며 유당, maltose, mannitol, xylose, L-arabinose 등을 이용하여 97% Hafnia alvei, 2% Escherichia coli로 동정되었다. Polymerase chain reaction으로 16S rRNA유전자를 증폭하여 1,426 bp의 염기서열을 결정하여 기존에 보고된 유전자들과의 유사도를 조사한 결과 분리된 균주 AS-20은 Hafnia alvei와 99%의 염기서열상 동성을 보였다. 이러한 결과는 BioMerieux Vitek Gram negative identification card 키트로 동정한 결과와 일치하였다. AS-20을 $10^{\circ}C,\;20^{\circ}C,\;30^{\circ}C$에서 배양하면서 $\beta-galactosidase$ 활성을 조사한 결과 저온인 $10^{\circ}C$와 $20^{\circ}C$에서 배양하였을 때에 배양최적 온도인 $30^{\circ}C$에서 배양했을 때 보다 1.5 배 정도 높은 효소활성을 보여주었으며, $30^{\circ}C$에서 배양된 대장균 보다 6배 이상의 효소활성을 보여 주어 저온조건에서 분리균의 효소생산이 비교적 높은 것으로 판단되었다. Galactose joined to glucose by a $\beta(1\rightarrow4)$ glycosidic bond makes lactose and this disaccharide is rich in milk. It is known that lacotse is hydrolyzed to each monomeric sugar by either lactase in human or $\beta-galactosidase$ in bacteria. Ingestion of milk by lactase-deficient persons causes a temporary diarrhea and subsequent chronic diarrhea results in colitis with chronic inflammation. We isolated a $\beta-galactosidase$ producing psycrotolerant strain AS-20 from near cattle shed and investigated the growth at various temperature conditions. Whereas Escherichia coli strains did not grow at $10^{\circ}C$, the AS-20 strain could grow well at this low temperature and showed optimal growth at $30^{\circ}C$. The isolated strain was identified as 97% Hafnia alvei by biochemical properties. This strain could ferment glucose, lacotse, maltose, mannitol, xylose, ONPG, rhamanose and L-arabinose, and decarboxylate lysin and ornithine. To confirm the identity of isolated strain we amplified 16S rDNA by PCR and searched similarity of the 1426 bp DNA sequcence with Genbank database. The strain AS-20 showed 99% similarity with Hafnia alvei. The activity of $\beta-galactosidase$ was 1.5 times higher when the cell was grown at 10 or $20^{\circ}C$ than at $30^{\circ}C$. The highest enzyme activity of AS-20 was also much higher than that of E. coli, which was grown at $30^{\circ}C$.
Instrumental case in Korean and Lithuanian:a contrastive analysis
박정운 한국외국어대학교 언어연구소 2005 언어와 언어학 Vol.0 No.36
This paper aims to investigate, in a contrastive way, various functions of the instrumental case in Korean and Lithuanian, which are not related to each other genetically and geographically. The functions to be examined are, among others, instrument, means, manner, cause, material, goal, status resulting from change, and various types of adverbials. The Korean and the Lithuanian instrumental case show overall similarities in these functions, though there are some minor differences specific to each language.
박정운 한국통역번역학회 2013 통역과 번역 Vol.15 No.1
This study aims to explore, from the perspective of cognitive linguistics, ways to investigate the translation of frame knowledge and metaphor in translation studies. It first briefly introduces frame semantics and conceptual metaphor theory. It proposes four major patterns and some minor patterns of frame relations between source and target languages, explicates their characteristics, and examines various aspects of translation of frame knowledge. It also proposes twelve patterns of metaphor relations between source and target texts, examines their characteristics, and discusses several aspects of metaphor translation. These patterns could be utilized in analyzing the translation of frame knowledge and metaphor in translation studies.