Antiviral Properties of Probiotic Mixtures against Rotavirus in the Rat

박재은,이도경,김민지,김경태,최경순,서재구,하남주,Park, Jae Eun,Lee, Do Kyung,Kim, Min Ji,Kim, Kyung Tae,Choi, Kyung Soon,Seo, Jae Goo,Ha, Nam Joo The Microbiological Society of Korea 2014 미생물학회지 Vol.50 No.4

로타바이러스는 선진국과 개발도상국의 영 유아에게 급성위장염을 일으키는 주요원인이다. 위장질환의 치료를 위한 유산균의 사용은 안전하며 간단하게 이용할 수 있다. 본 연구는 Sprague-Dawley 랫드에서 유산균혼합물의 로타바이러스에 대한 항 바이러스 효능을 조사하였다. 24마리의 새끼와 그들의 어미를 무작위로 네 그룹으로 나누었다; placebo, phosphate buffered saline (PBS)와 유산균 혼합물-1, 유산균 혼합물-2 그룹. 5일령인 모든 랫드에게 8 log plaque forming units의 농도로 로타바이러스를 접종하고 유산균혼합물-1, 유산균 혼합물-2 그룹은 4일 동안 하루에 한번 각각 8 log colony forming units의 농도로 유산균 혼합물을 경구 투여하였다. 대조군인 placebo와 PBS 그룹은 4일 동안 하루에 한번 각각 동일한 양의 placebo (말토오스, 폴리덱스트로스 포함)와 PBS를 경구 투여하였다. 항 바이러스 효능분석을 위해 Real-time quantitative PCR (RT-qPCR)과 소장융모관찰을 수행하였으며, 그 결과 유산균 혼합물-1, 유산균 혼합물-2 그룹의 소장무게는 대조군 보다 무거웠다. 대조군의 융모는 길이가 짧아지고 융모상피세포의 괴사가 일어났지만 유산균 혼합물-1과 유산균 혼합물-2 그룹에서는 이러한 형태학적 변화를 관찰할 수 없었다. RT-qPCR 분석에서는 유산균 혼합물-1과 유산균 혼합물-2 그룹의 분변샘플과 소장상피세포에서 로타바이러스의 VP7 유전자 레벨이 낮았다. 이러한 연구결과는 유산균혼합물이 로타바이러스 위장염에 대한 대체요법이나 치료에 유용하게 사용될 수 있음을 시사한다.

충북산 토종고추의 계통별 품질 분석

박재은,박혜진,강혜정,김성겸,신현만,김흥태,엄현주,Jae Eun Park,Hye Jin Park,Hye Jeong Kang,Seong Kyeom Kim,Hyun-Man Shin,Heung Tae Kim,Hyun-Ju Eom 한국식품영양학회 2024 韓國食品營養學會誌 Vol.37 No.2

This study examined the quality characteristics of 10 different lines of native peppers collected from organic farms in Chungbuk province. The study found a strong correlation between the redness (a*) and ASTA values, which both contribute to the perceived quality of peppers. The highest values were observed in the Iyugsa line. The content of capsaicinoid and beta-carotene also showed a positive correlation, with Chilsungcho having the highest statistically significant value. While the total polyphenol content did not correlate with the other indicators, Chilsungcho again had the highest levels. The Yuwolcho line exhibited the highest ABTS radical scavenging ability, while the Eumseongcho line showed the highest DPPH radical scavenging ability. Taking into account the overall bioactivity quality, Chilsungcho had the highest values in terms of total polyphenol, beta-carotene, capsaicinoid, and redness. It also had the second highest total flavonoid content, ABTS, and DPPH radical scavenging activity, all statistically significant. Therefore, Chilsungcho can be considered an excellent choice when considering physiological activity. Furthermore, this study provides valuable information about the unique characteristics of these 10 native pepper lines, which can assist in selecting the appropriate pepper for food manufacturing and serve as a helpful resource for future research.

들깨 대실/잎들깨1호 재조합 자식계통(RILs)의 농업적 특성 및 품질 분석

박재은,이명희,오기원,김성업,오은영,하태정,조광수,정찬식,김정인,Park, Jae Eun,Lee, Myoung Hee,Oh, Ki Won,Kim, Sungup,Oh, Eunyoung,Ha, Tae Joung,Cho, Kwang-Soo,Jung, Chan Sik,Kim, Jung In 한국작물학회 2021 한국작물학회지 Vol.66 No.3

This study was conducted to obtain basal information for the development of perilla cultivars with improved quality. The F₇ population of recombinant inbred lines (RILs) from a cross between the parents of Daesil for seeds and Ipdeulkkae1 for vegetables was used as the material. We evaluated several agricultural characteristics and seed quality. Variations were observed in most of the measurements; for example, stem length (ranging from 66.0 to 150.0 cm), number of branches (from 5 to 23), flower cluster length (ranging from 5.1 to 10.5 cm), number of flower clusters (from 17 to 131), a-linolenic acid content (from 54.2 to 64.1%), and functional compound content (rosmarinic acid 869.5~3,508.1 ㎍/g; luteolin 47.4~864.3 ㎍/g; apigenin 57.1~296.7 ㎍/g) all showed variation. Significant correlations between stem length and the number of branches (0.561) and number of branches versus number of flower clusters (0.638) were detected in the RIL F₇ population. Most agricultural characteristics and seed qualities showed a normal distribution with large variation, and transgressive segregation was observed in many descendants with characteristics to those of their parents. Daesil/Ipdeulkkae1 RIL F₇ populations could be useful for future QTL analysis as well as for intermediate breeding lines for high-quality perilla cultivars.

다양한 약물과 영양제가 장내세균에 미치는 영향

박재은,이도경,하남주,송영천,Park, Jae Eun,Lee, Do Kyung,Ha, Nam Joo,Song, Young Cheon 한국미생물학회 2015 미생물학회지 Vol.51 No.1

Recently, change of Western pattern diet and lifestyle is caused by various metabolic disorders and chronic diseases. These diseases need to take medicine regularly. Also, many people take health functional food, various vitamins and nutritional supplements in order to maintain a healthy life. But, there was no study about affects taking medicines against bacteria with gastrointestinal relevance. This study was performed by antibacterial activity test to evaluate the influence of a long time or commonly used medication. As a result, medicines of Vitamins & Minerals or Central nervous system show antibacterial activity against beneficial enteric bacteria and harmful enteric bacteria. Dexibuprofen of the Anti-inflammatory Drugs that acts on the central nervous system has shown high antibacterial activity at beneficial enteric bacteria strains (Lactobacillus casei, Lactobacillus rhamnosus) and harmful enteric bacteria (Staphylococcus aureus). Also, fenofibric acid of the antilipemic agents that acts on the Cardiovascular & Hematopoietic system has shown high antibacterial activity at beneficial enteric bacteria strains (Lactobacillus casei). Vitamins & Minerals appeared antibacterial activity against most intestinal bacteria. Vitamin B-Complex/with C and vitamin C were especially high with beneficial enteric bacteria strains (Bifidobacterium infantis) and harmful enteric bacteria (E. coli, E. aerogenes, S. flexneri, S. Typhimurium, S. aureus). Therefore, these results indicate that variously taking medicines have generally antibacterial activity against harmful enteric bacteria strains and beneficial enteric bacteria strains.

해양 정합장처리에서 매개변수 오정합과 바이어스

박재은,김재수,신기철,Park Jae-Eun,Kim Jea-Soo,Shin Kee-Cheol 한국음향학회 2005 韓國音響學會誌 Vol.24 No.2

해양환경에서 정합장처리를 통해 음원위치를 추정할 경우 실측신호의 음장과 복제음장 사이에 각종 매개변수의 오정합이 발생하여 상관값의 저하와 함께 음원 위치추정 결과에 바이어스를 발생시킨다. 본 논문에서는 정합장처리를 이용한 음원 위치추정에서 해양환경 및 시스템 오정합 매개변수로 인한 바이어스와 상관값의 변화 그리고 민감도에 대해서 고찰하였다. 이를 위해 Pekeris 해양 도파관에서 수치실험을 수행하였으며, 그 결과 매개변수 오정합이 음원 위치에서의 바이어스와 상관값의 감소를 발생시켰다. 또한 매개변수 민감도 평가를 통해 배열의 경사와 수심, 해저면 수심과 해저층의 음속이 민감도가 높음을 확인 할 수 있었다. In matched field processing (MEP), the observed acoustic field data is basically correlated with the replica produced by the modeling. therefore the results of source localization and correlation is limited by the mismatch of the environment and sensor location. In this paper. the effects of mismatch in environment and system on the bias in estimating the source location are investigated in the context of source localization. In the Pekeris waveguide, the simulation shows that the mismatches in environment and system, can cause a significant biases in the source localization and a degradation in MFP correlation. Mismatch caused by uncertainties in array tilt and depth, bottom depth, bottom sound speed, etc. causes degradation in source localization performance.

탄산리튬으로부터 수산화리튬 전환을 위하여 탄산리튬의 열분해에 대한 연구

박재은,박민화,서형준,김태성,김대원,김보람,최희락,Park, Jae Eun,Park, Min Hwa,Seo, Hyeong Jun,Kim, Tae Seong,Kim, Dae Weon,Kim, Bo Ram,Choi, Hee Lack 한국결정성장학회 2021 한국결정성장학회지 Vol.31 No.2

Research on the production of lithium hydroxide (LiOH) has been actively conducted in response to the increasing demand for high nickel-based positive electrode materials for lithium-ion batteries. Herein we studied the conversion of lithium oxide (Li2O) through thermal decomposition of lithium carbonate for the production of lithium hydroxide from lithium carbonate (Li2CO3). The reaction mechanism of lithium carbonate with alumina, quartz and graphite crucible during heat treatment was confirmed. When graphite crucible was used, complete lithium oxide powder was obtained. Based on the TG analysis results, reagent-grade lithium carbonate was heat-treated at 700℃, 900℃ and 1100℃ for various time and atmosphere conditions. XRD analysis showed the produced lithium oxide showed high crystallinity at 1100℃ for 1 hour in a nitrogen atmosphere. In addition, several reagent-grade lithium oxides were reacted at 100℃ to convert to lithium hydroxide. XRD analysis confirmed that lithium hydroxide (LiOH) and lithium hydroxide monohydrate (LiOH·H2O) were produced.

능동 소나 체계에서의 표적 탐지거리 예측 알고리즘과 최적 탐지깊이 결정에의 응용

박재은(Jae-Eun Park),김재수(Jae-Soo Kim) 한국해양공학회 1994 한국해양공학회지 Vol.8 No.1

In order to estimate the detection range of a active SONAR system, the SONAR equation is commonly used. In this paper, an algorithm to calculate detection range in active SONAR system as function of SONAR depth and target depth is presented. For given SONAR parameters and environment, the transmission loss for given source is computed for each receiver depth and range. Once the transmission loss and background level are found, signal excess is computed. Using log-normal distribution, signal excess is converted to detection probability at each range. Then, the detection range is obtained by integrating the detection probability as function of range for each depth.<br/> The proposed algorithm have been applied to the case of omni-directional source with center frequency 30Hz for summer and winter sound profiles. It is found that the optimal search depth is the source depth since the detection range increase at source depth where the signal excess is maximized.

제주도 내 스타벅스의 지리 정보를 활용한 카페 입지 유형 탐색

박재은(Jae Eun Park) 경희대학교 학술단체협의회 2021 고황논집 Vol.69 No.-

Gelidium amansii Extract, a Potent α-glucosidase and α-amylase Inhibitor, Alleviates Postprandial Hyperglycemia in Diabetic Mice

Jae-Eun Park(박재은),Jung-Min Kim(김정민),Ji-Sook Han(한지숙) 한국생명과학회 2017 생명과학회지 Vol.27 No.9

우뭇가사리(Gelidium amansi)는 홍조류로서 우뭇가사리과(Gelidiaceae)에 속한다. 현재까지 우뭇가사리의 항산화 및 항비만 효과 등의 기능들이 연구되었으나 식후 혈당 수치에 미치는 영향에 관한 연구는 부족한 실정이다. 이에 본 연구에서는 Gelidium amansii extract (GAE)가 탄수화물 가수분해 효소(α-glucosidase, α-amylase)에 미치는 억제 효과 및 streptozotocin (STZ)으로 유도된 당뇨병 마우스의 식후고혈당에 미치는 완화효과를 조사하였다. 정상군과 STZ으로 유도된 당뇨병 마우스에 수용성 전분(2 g/kg body weight)을 경구투여한 후 GAE (300 mg/kg body weight) 또는 acarbose (100 mg/kg body weight)를 단독 또는 함께 투여하였다. 혈당은 꼬리채혈을 통해 0, 30, 60, 120분 간격으로 측정하였다. α-glucosidase와 α-amylase에 대한 GAE의 IC50 값은 각각 0.099±0.009 mg/ml와 0.178±0.038 mg/ml의 결과값을 나타내어, 양성대조군인 acarbose보다 더 효과적이었다. STZ으로 유발된 당뇨병 마우스의 식후 혈당 수치는 대조군에 비해 GAE 투여 시 유의적으로 더 낮았다(p<0.05). 또한 GAE 투여는 당뇨병 마우스에서 포도당 반응에 대한 곡선하면적 감소와 관련이 있었다(p<0.05). 이러한 결과는 GAE가 α-glucosidase, α-amylas와 같은 탄수화물 가수분해 효소를 억제함으로써 식후 고혈당을 완화시키는 유용한 천연기능성 식품이 될 것으로 사료된다. Gelidium amansii shows antioxidant and anti-obesity effects; however, the effect on postprandial blood glucose levels is not known. The objective of the present study was to investigate the inhibitory effect of Gelidium amansii extract (GAE) on carbohydrate-digesting enzymes and its ability to alleviate postprandial hyperglycemia in streptozotocin (STZ)-induced diabetic mice. Gelidium amansii was extracted with 80% ethanol and concentrated for use in this study. The α-glucosidase and α-amylase inhibition assays were performed using the colorimetric method. ICR normal and STZ-induced diabetic mice were orally administered GAE (300 mg/kg body weight) or acarbose (100 mg/kg body weight) alone or soluble starch (2 g/kg body weight). Blood samples were taken from the tail vein at 0, 30, 60 and 120 min. Our results indicated that GAE markedly inhibited α-glucosidase and α-amylase activities with IC50 values of 0.099±0.009 mg/ml and 0.178±0.038 mg/ml, respectively, and was a more effective inhibitor than acarbose, the positive control. Further, the postprandial blood glucose levels of STZ-induced diabetic mice in the GAE-administered group were significantly lower than those of control group mice (p<0.05). Moreover, the area under the curves (AUC) significantly decreased with GAE administration in STZ-induced diabetic mice (p<0.05). These results indicate that GAE may be effective in decreasing postprandial blood glucose levels by inhibiting carbohydrate-digesting enzymes such as α-amylase and α-glucosidase. Therefore, GAE could be used as a potential functional food for alleviating postprandial hyperglycemia.

안정화 처리된 폐광산 토양의 생태기능상태 평가를 위한 효소활성도 및 비소호흡유전자의 적용

박재은(Jae Eun Park),이병태(Byung-Tae Lee),이상우(Sang Woo Lee),김순오(Soon-Oh Kim),손아정(Ahjeong Son) 大韓環境工學會 2017 대한환경공학회지 Vol.39 No.5

폐광산은 방치된 광미 등으로 인하여 주변환경에 복합적인 중금속 오염을 야기한다. 이를 방지하기 위하여 국내에서는 1990년대 중반부터 석회석 등의 안정화제와 복토를 이용한 안정화 공법을 기반으로 토양개량사업이 시행 중이다. 복원된 토양의 상부에서는 작물의 재배로 인해 중금속이 고정된 안정화 층이 지화학적 변화를 겪게 되며 이에 따른 중금속의 용출 및 이동이 가능하므로 토양개량사업을 마친 토양에 대한 질 평가 등의 사후 관리는 반드시 필요하다. 토양의 질 평가를 하기 위해서는 이화학적 분석 또는 생물학적인 분석을 개별적으로 하기보다는 이들을 결합한 종합적인 분석이 필요하며 이를 통해 토양의 생태기능상태(ecological functional state)를 평가할 수 있다. 본 연구에서는 대상시료로 경상북도 봉화군 풍정 광산, 전라남도 광양시 점동 광산, 충청남도 서산시 서성 광산 인근 안정화 처리 토양과 안정화 처리가 되지 않은 오염, 비오염 토양을 선정하였다. 토양의 이화학적 성질인 pH, CEC, LOI와 중금속 농도를 측정하였고, 미생물 효소활성도와 비소환원유전자를 정량하였다. 다변량 통계분석을 바탕으로 모든 데이터를 분석하여 토양 생태기능상태를 평가하였다. 안정화 심도 토양과 상부복토, 하부오염토 간의 상관관계를 확인한 결과, 안정화 심도 토양에서 중금속의 농도가 높게 측정되었다. 그리고 풍정광산에서는 안정화 처리 심도 토양이 하부오염토와 유사한 특성을, 점동, 서성 광산에서는 안정화 심도 토양이 상부복토와 유사한 특성을 나타내었는데 이는 점동, 서성 광산 주변 상부복토의 생태기능상태가 좋지 않을 수 있음을 시사한다. Heavy metals leaching from closed mines have been causing severe environmental problems in nearby soil ecosystems. Mine reclamation in Korea has been recently implemented based on the heavy metal immobilization (a.k.a., stabilization). Since the immobilization temporarily fixes the heavy metals to the soil matrix, the potential risk of heavy metal leaching still exists. Therefore the appropriate monitoring and the related policies are required to safeguard the soils, where all the cultivations occur. The current monitoring methods are based on either heavy metal concentration or simple toxicity test. Those methods, however, are fragmented and hence it is difficult to evaluate the site in an integrated manner. In this study, as the integrated approach, ecological functional state evaluation with a multivariate statistical tool was employed targeting physiochemical soil properties, heavy metal concentrations, microbial enzymatic activity, and arsenic respiratory reductase gene quantity. Total 60 soil samples obtained from three mines (Pungjeong, Jeomdong, Seosung) were analyzed. As a result, the stabilized layer soil and lower layer soil have shown the similar pattern in Pungjeong mine. In contrast, Jeomdong and Seosung mine have shown the similarity between the stabilized layer soil and the cover layer soil, indicating the possible contamination of the cover layer soil.