Porphyromonas gingivalis, Prevotella intermedia, 그리고 Prevotella nigrescens에서의 hemin 결합 단백질에 대한 연구

김성조,Kim, Sung-Jo 대한치주과학회 2006 Journal of Periodontal & Implant Science Vol.36 No.1

The results of this study confirm that the availability of hemin influences the expression of selected membrane proteins of Porphyromonas gingivalis, Prevotella intermedia, and Prevotella nigrescens. A 30 kDa (heated 24 kDa) hemin-binding protein whose expression is hemin regulated was identified and purified in P. gingivalis. A strong hemin-binding function was found by LDS-PAGE and TMBZ staining when P. gingivalis cells were grown under hemin-limited conditions. A 50 kDa cell envelope associated protein, whose expression is hemin regulated, is considered to be a putative hemin binding protein from P. intermedia and P. nigrescens, respectively. N-terminal amino acid sequence analysis of CNBr-digested 24 kDa hemin binding protein from P. gingivalis revealed that this protein belongs to a new, so far undescribed hemin-binding class of proteins. N-terminal amino acid sequence of a 50 kDa putative hemin binding protein from P. intermedia was identical with Enolase from Streptococcus intermedia. Work is in progress to further characterize the molecular structure of these proteins.

전봉건 시에 나타난 존재인식과 초월 연구 - 시집 『돌』을 중심으로

김성조 한국문예창작학회 2006 한국문예창작 Vol.5 No.1

Bentazon 連用에 의한 沓多年生 雜草의 防除

金成朝 圓光大學校 1982 論文集 Vol.16 No.2

Bnetazon의 效果的인 使用方法을 究明하기 위하여 劑型, 使用時間, 湛水 및 落水條件 等으로 나누어 連 3年間에 걸쳐 單用, 組合 및 混合處理하여 實驗을 實施하였다. 1. Bentazon의 落水條件下 處理는 너도방동산이, 올방개, 올미 等의 多年生雜草에 對하여 固定된 優秀한 防除效果가 있음을 連用實驗에서 認定되었다. 2. Bentazon은 處理年度에 따라 防除效果에 若干의 變動이 있었다. 3. Bentazon粒劑處理는 移秧 15日後 落水處理가 바람직 하였다. 4. Bentazon單劑 1回 處理로서는 피를 비롯한 一年生雜草의 一部를 防除할 수 없으므로 avirosan 4.4/1.1g, 3kg/10a를 移秧 10日 後에 處理하고 뒤이어 移秧25日後에 Bentazon 48% 液劑 300cc/10a의 處理나 10Gk 3g/10a를 處理하는 것이 3年間의 繼續實驗에서 가장 效果的이었다. To establish the effective weed control methods with bentazon(3-isopropyl-1H-2,1,3,-benzothiadizine-(4)3H-one 2,2-dioxide) in paddy rice, the field experiment was conducted at different formulations, different application time, and flooded and drainage conditions by the continuous use of bentazon alone and /or combination and incorporation with other chemicals during three years. 1. The herbicidal activity of bentazon solution on perennials such as Cyperus serotinus Rottb, Sagittaria pygmaea Mig. and Eleocharis kuroguwai Ohwi was very high when was applied once a year from 1977 to 1979 under drainage condition. 2. The weeding effect of bentazon alone was somewhat shift according to the applied years. 3. Bentazon granule was most desirable when applied 15 days after transplanting(DAT) under drainage condition. 4. As a few annuals uncluding barnyard grass (Echinochloa crusgalli Beav.) cannot be controlled by a single treatment of bentazon granule in the experiments of the continuous use over three years, piperophos+dimethametryn [avirosan;n-(0,0-dipropyl-dithiophosphoryl-acetyl)-2-methyl piperizine+2-methylthio-4-(1,2-dimethyl propyl-amino)-6-ethylamino-s-triazine] 4.4/1.1G at 3kg/10a was most effective on annual and perennial weeds at the time of 10 DAT.

흰쥐 腦에서의 Cholecystokinin Octapeptide의 分布

김성조,홍해숙,주강 慶北大學校 醫科大學 1986 慶北醫大誌 Vol.27 No.1

흰쥐 뇌에서 cholecystokinin-octapeptide(CCK-8)의 분포를 관찰하기위하여 간접면역형광법을 행하여 관찰해 본 결과 다음의 많은 부위에서 CCK-8양성세포 및 양성섬유를 관찰할 수 있었다. 대뇌피질 : 신피질, 전후핵, 이상엽, 후내야, 해마형성에서 공히 CCK-8양성세포 및 양성섬유가 분포하였다. 중격야 : CCK-8양성섬유가 외측중격핵, n. of accumbens, 분계조의 기저핵, 브로카의 대각핵에서 관찰되었다. 선조체 : n. caudatus putamen에서 CCK-8양성섬유가 존재하였다. 간뇌 : 시상의 경우 전내측시상핵, n. reuniens, n. periventricularis rotundocellularis, 외측슬상체, 내측슬상체에서 CCK-8양성섬유가 관찰되었고 시상하부의 경우 CCK-8양성세포가 시색상핵, 실방핵, 배내측시상하부핵 및 유두상핵에 존재하였고 양성섬유는 내측시속전야, 배내측시상하부 및 복내측시상하부에 분포하였다. 편도체 : CCK-8양성세포는 외측, 기저 및 피질편도핵에서 관찰되었고 양성섬유는 내측, 외측, 기저, 피질편도핵에서 관찰되었다. 중뇌 : 흑질, 복측피개야, n. linea rostralis, 중심회백질, n. parabrachialis colliculi posterioris, 하구 및 외측모대핵에서 CCK-8양성세포가 관찰되었고 양성섬유의 경우 각간핵, n. linea rostralis, 복측피개야, 흑질, 상구, 중심회백질, n. parabrachialis colliculi posterioris, parabigeminal n., 하구, 외측모대핵 및 배측봉선핵에서 관찰되었다. 후뇌 : CCK-8양성세포는 n. parabrachialis lateralis, n. of group O, 고속핵, 최후야 및 삼차신경척수로핵에서 분포하였고 양성섬유는 교봉선핵, 교핵, n. parabrachialis lateralis, 청반핵, n. parolivaris superior, 와우신경핵, 고속핵, 미주신경배측핵, inferior olivary complex, 박속핵, 설상속핵, 삼차신경척수로핵 및 망향체의 일부등에서 존재하였다. In order to investigate the distribution of immunoreactive cholecystokinin octapeptide (CCK-8) containing neurons in the brain of the rat, brains of the rats perfused with fixative were extirpated, frozen sectioned and processed for indirect immunofluorescent technique. The results obtained were as follows. Cortex CCK-8 containing cells and fibers were found in the neocortex, anterior olfactory nucleus (n.) piriform cortex, entorhinal cortex and hippocampal formation. Septal Area CCK-8 containing fibers were present in the lateral septal n., n. of accumbens, basal n. of stria terminalis and n. of diagonal band. Corpus Striatum CCK-8 containing fibers were demonstrated in the n. caudatus putamen. Diencephalon In the anterior medial thalamic n., n. reuniens, n. periventricularis rotundocellularis, lateral geniculate body and medial geniculate body, CCK-8 containing fibers were found. In the hypothalamus, CCK-8 containing cells were present in the supraoptic n., paraventricular n., dorsomedial hypothalamic n. and supramammillary n., and fibers were observed in the medical preoptic n., dorsomedial hypothalamic n. and ventro medical hypothalamic n. Amygdaloid Complex CCK-8 containing cells were distributed in the lateral, basal and cortical n., and fibers were present in the medial, lateral basal and cortical n.. Mid Brain CCK-8 containing cells were found in the ventral tegmental area, substantia nigra, n. linea rostralis, substantia grisea centeralis, n. parabrachialis colliculi posterioris, inferior colliculus and lateral lemmiscus, and fibers were distributed in the interpeduncular n., n. linearostralis, ventral tegmental area, substantia nigra, superior colliculus. substantia gricea centralis, n. parabrachialis colliculi posterioris, parabigeminal n., inferior colliculus, lateral lemniscus, and n. raphe dorsalis. Pons, Medulla Oblongata CCK-8 containing cells were detected in the n. parabrachialis lateralis, n. of group O, n. tractus solitarii, area postrema and n. tractus spinalis nervi trigemini, and fibers were found in the n. centralis superior, pontine n., n. parabrachialis lateralis, locus coeruleus, n. Parolivaris superioris, ocochlear n., n. tractus solitarii, dorsal n. of vagus nerve, inferior olivary complex, n. gracilis, n. cuneatus, n. tractus spinalis nervi trigemini and reticular formation.

Prevotella intermedia와 Prevotella nigrescens의 세균내독소에 대한 연구;화학적 분석 및 면역생물학적 활성 평가

김성조,Kim, Sung-Jo 대한치주과학회 2004 Journal of Periodontal & Implant Science Vol.34 No.2

The purpose of this study was to assess some biological activities of lipopolysaccharides (LPSs) from P. intermedia and P. nigrescens. LPS was prepared by the standard hot phenol-water method. NO production was assayed by measuring the accumulation of nitrite in culture supernatants. $TNF-{\alpha}$ production was determined by enzyme-linked immunosorbent assay. Western blot analysis of iNOS and analysis of reverse transcription (RT)-PCR products were carried out. LPS from P. intermedia demonstrated higher KDO content than those from two stains of P. nigrescens. LPSs from P. intermedia and P. nigrescens were mitogenic for spleen cells of BALB/C mouse. The present study clearly shows that LPSs from P. intermedia and P. nigrescens fully induced iNOS expression and NO production in RAW264.7 cells in the absence of other stimuli. Moreover, LPSs from P. intermedia and P. nigrescens clearly induced $TNF-{\alpha}$ production in RAW264.7 cells. The biological activities of LPS from P. intermedia was found to be comparable to those of P. nigrescens LPS. The ability of LPSs from P. intermedia and P. nigrescens to promote the production of NO and $TNF-{\alpha}$ may be important in the pathogenesis of inflammatory periodontal disease.

「SUPERCOMPUTING」특집을 내면서

김성조,유관종,유승화 한국정보과학회 1991 정보과학회지 Vol.9 No.4

논 土壤中 重金屬含量이 水稻의 生育, 收量 및 吸收에 미치는 影響

金成朝 圓光大學校 1983 論文集 Vol.17 No.2

農業環境汚染物質 特히 土壤吳染物質의 하나인 重金屬元素 Cd, Pb, Cr, Cu, Zn, Mn, Ni 等을 土壤에 處理濃度를 달리하여 湛水條件下에서 水稻를 栽培한 Pot에 의한 土耕試驗을 實施하여 生育狀況, 數量, 植物體內에 吸收程度 等을 調査한 結果는 다음과 같다. 1. 處理濃度 增加에 따라 供試한 重金屬들에 의한 水稻 生育抑制現象이 增大하였고, 特히 Cr과 Ni에서 그 現象이 뚜렷하였다. 2. 水稻生育抑制에 미치는 重金屬元素들의 影響은 Ni(??)Cr>Cu(??)Cd>Pb>Zn =Mn順으로 높았다. 3. 處理濃度 30ppm에서 收量減少 程度는 Ni>Cr>Cu>Pb>Cd의 순이었고, Zn과 Mn구의 收量은 對照區와 비슷하였다. 4. 25%의 收量減少를 나타낸 重金屬元素의 處理濃度는 Ni : 12ppm, Cr : 30ppm, Cu : 60ppm, Pb : 100ppm區였고, Cd, Zn, 및 Mn 處理區에서는 25% 收量減少 濃度를 찾기 어려웠다. 5. 植物體中에 吸收된 重金屬含量은 根 > 莖葉 > 玄米의 順이었으나, Mn만은 例外的으로 莖葉 > 根 > 玄米의 順이었다. To investigate the effects of heavy metals on the growth and yield of rice and to measure the amounts of heavy metals in rice plant (Oryza sativa var. 'Milryang No.15'), the pot experiments were conducted under the flooded paddy soils treated with various concentrations of heavy metals (Cd, Pb, Cr, Cu, Zn, Mn, Ni). The results obtained were as follows : 1. According to the increase of the concentration of all the tested heavy metals, the growth of rice plant was decreased and especially the trends was appeared to be clear in Cr and Ni treatments. 2. The inhibition effect of heavy metals on the growth of rice plant was in the order of Ni(?>?)Cr>Cu(??)Cd>Pb>Zn=Mn. 3. At the concentration of 30ppm of each heavy metal, the order of yield decrement was Ni>Cr>Cu>Pb>Cd. The yield in the plots treated with Zn and Mn was similar to the control plot. 4. 25% yield decrement showed in the concentration of 12ppm Ni, 30ppm Cr, 60 ppm Cu and 100ppm Pb, respectively. 5. The amounts of heavy metals absorbed by rice plant were generally in the order of root>stem and leaf>brown rice, but was stem and leaf>root>brown rice in case of Mn treatment.

보철 및 Implant와 관련된 치주과 영역의 처치

김성조 대한치과의사협회 1996 대한치과의사협회지 Vol.34 No.10

Allium 속의 Cd 및 Zn 흡착력

김성조,백승화,문광현 한국식품영양학회 1996 韓國食品營養學會誌 Vol.9 No.3

본 연구는 Allium속의 중금속 흡착력을 구명하고자 식물체의 식용부위별, 시료의 입자별, 중금속의 농도별, 용액의 온도별, pH별 실험 결과이다. Allium속에 의한 중금속의 흡착 량은 Allium속의 종류에 관계없이 Cd이 Zn보다 훨씬 많았다. 대파, 쪽파는 입자가 클 수록 Cd 흡착이 잘 되었으나 Zn은 입자별 차이가 나타나지 않았다. 농도별 중금속 흡착력은 용액중의 농도가 높을수록 증가하였으나 증가율은 일정하지 않았다. 온도에 따른 중금속의 흡착력은 온도 증가에 따라 증가하였으나, 예외적으로 달래는 Cd 흡착량이 감소하였다. pH에 따른 중금속의 흡착량은 두 금속 모두 큰 영향을 받지 않았다. 중금속의 흡착에 미치는 Allium속 성분함량간의 관계는 함황 아미노산과 비타민 B_2에서 유의성이 인정되었다. The study was performed under the various conditions, such as the edible parts and particle sizes of Allium, the concentrations, the temperatures, and the pH of heavy metal solutions to investigate their adsorption capacity of heavy metals by genus Allium. The adsorption amount of Cd by Allium in the aqueous solution was apparently higher than that of Zn by them. The larger the particle size of welsh onion and shallot was, the higher the adsorption of Cd was. But the adsorption ratio was not different. As the temperature increased, the amount of heavy metal adsorption increased in general, but the adsorption of Cd by wild garlic, Zn by garlic decreased. Adsorption of Cd and Zn to Allium was not affected by the various pH. The correlation between the amount of components in edible parts of Allium and that of adsorption of heavy metals was significantly high in amino acids containing sulfhydryl radical(-SH) vitamin B_2.

결명자 첨가식이가 흰쥐의 체내 카드뮴 축적에 미치는 영향

김성조,백승화,허종욱,김운성,이주돈,강경원,박성혜,한종현,정성윤,이승현 동아시아식생활학회 2002 東아시아食生活學會誌 Vol.12 No.6

The purpose of this study is to investigate the effect of raw Cassia tora L. powder added-diets on reducing cadmium accumulation in rats. The experimental animals were Sprague-Dawley family(♂ , 4 weeks) which was classified into normal group CN, compared group CS, Cd-added group C1 and groups C2, C3. C4 in which 0.5, 1.0 and 1.5% of the Cassia tora L. powder are added, respectively. The growth rate and food efficiency ratio, and the amounts of accumulated cadmium in rats for 8 weeks were measured and analyzed. The results are as follows; 1. The rates of weight gain decreased in the order of C3>C2>C4>Cn>Cs>C1 groups, and C1 group to which only cadmium water had been fed was the lowest among them. The correlation between groups C1 and C3 was significantly different at the 1% level. 2. Food efficiency ratio(FER) decreased in the order of C3>C2>Cs>Cn>C4>C1, and the FERs of C3, C2, CS, CN and C4 are greater than that of C1 by 22.87, 19.89, 18.54, 14.20 and 13.17%, respectively. 3. As fix the Cassia tora L. powder-added groups, the amounts of cadmium accumulated in organs and tissues that is, the brain, heart, spleen, liver, lungs, testicles, kidney, femoral muscle and leg bones were 0.45±0.04 to 0.83±.04, 1.68±0.02 to 2.16±0.02, 3.26±0.05 to 4.62±0.27, 37.82±0.09 to 47.71±0.73, 1.07±0.10 to 1.66±0.04, 1.04±0.06 to 1.24±0.08, 36.79±0.20 to 39.61±0.53, 0.87±0.02 to 1.00±0.02 and 0.65±0.17 to 1.27±0.06 ㎍/g respectively. 4. The accumulated Cd content for C4 was the lowest among Cassia tora L. powder-added groups. When the results for C4 are compared with those for C1, it is observed that each cadmium content accumulated in the brain, heart, spleen, liver, lungs, testicles, kidney, femoral muscle and leg bones is dropped by 49.03, 22.56, 36.02, 35.75, 41.75, 36.20, 37.00, 22.77 and 56.67%, respectively. On the other hand, the accumulated Cd content increased in the order of brain <leg bones <femoral muscle <testicles <lungs <heart <spleen <kidney <liver. 5. The average Cd accumulation rate in organs and tissues relative to the total Cd intake was 7.14% for C1, while they are 4.91, 4.81 and 4.509a for Cassia tora L. powder-added groups C2, C3 and C4, respectively. 6. The Cd content accumulated in the hair for C1 was the highest, and those for Cassia tora L. powder-added groups gradually increased until 6 weeks and decreased after that. 7. The Cd content accumulated in the feces for C4 was the highest, and those for the groups other than C3 and C4 gradually decreased until 6 weeks and increased after that.