Autoradiography 법에 의한 흰쥐 뇌의 Serotonin 표지 양상

朱剛 慶北大學校 醫科大學 1983 慶北醫大誌 Vol.24 No.1

총 10마리의 Sprague-Dawley rat의 뇌측실에 직접 10^-5 ^3H-5-HT 14㎕/min를 5분간 주사하여 autoradiography법으로 실험한 결과를 요약하면 다음과 같다. Raphe nucleus 에서 銀粒子가 가장 많이 발견되었으며 다음이 시상하부의 paraventricular nucleus 였고 dorsomedialis 와 suprachiasmaticus 에서도 상당히 나타났다. 대뇌피질에서는 cingulate 와 hi-ppocampus에서 발견되었고 전엽후반부에서도 조금 발견되었으며 나머지 부위는 거의 찾아볼 수 없었다. 이상을 요약해 보면 Serotonin의 뇌에서의 역할은 주로 뇌의 내분비적 역할이나 자율신경조절 역할을 담당하는 신경원에서 대부분 나타났으며 연합운동중추 영역에서 발견된 것은 앞으로 계속적인 연구가 필요하다고 본다. Serotonin was detected in individual neuclei of the rat brain by a autoradiographic method. This amine would be unevenly distributed throughout the areas examined. Sprague-Dawley rat was injected ^3H-5-HT (14㎕/min) for 5 min through intraventricle. Rat brain was fixed by glutaraldehyde and washed in ethanol. The staining was performed by thionin cresyl violet. The result are summerized as follows: Highest silver grain of serotonin was found in nuclei of Raphe. In the hypothalamus, the highest grain was found in paraventricular nucieus. In the cerebral cortex, the auther could find the grain in the cingulate, hippocampus and post frontal Gyrus. So in is assumed that serotonin is connected with the nuclei of the neuroendocrine or automatic system and synergic action system.

탄성 Braid의 특성에 관한 연구(II) ―탄성 Braid의 제조조건에 의한 특성변화―

주강,곽영식,Choo, Kang,Kwag, Young-Sig 한국섬유공학회 1985 한국섬유공학회지 Vol.22 No.4

Dependence of physical properties on manufacturing conditions of cotton elastic braid made of core latex rubber thread were studied experimentally. The results as follows; 1) Breaking strength had little effect on braiding angle($\theta$a), and elongation was proportional to tan $\theta$a. 2) Relation between limiting elongation and strain ratio of core latex rubber thread showed a linear function of which slope coresponds to a draft ratio in manufacturing process(Da). 3) Tensile stress was depended on $\theta$a. 4) Stress relaxation behaviour of the elastic braid satisfied the stress relaxation equation of polymerec elastomers as shown below. ln ft=lnfto-K ln n Where K values, rate of stress relaxation of the elastic braid, can be expresses as; K=(0.1101-0.7763u)k When considered the strain ratio of rubber thread, from this equation, calculation of the stress relaxation rate(k) of rubber thread enables the estimation of tensile stress at any time.

탄성 Braid의 특성에 관한 연구(I) ―탄성 Braid구조의 기하학적 이론―

주강,곽영식,Choo, Kang,Kwag, Young-Sig 한국섬유공학회 1985 한국섬유공학회지 Vol.22 No.4

An equation of structural changes in stretching cotton elastic braid was derived by modeling a structure of cotton elastic braid consisting of latex rubber thread as core material, and the structure changes under manufacturing conditions and the effect of the changes on the physical properties were studied. The results obtained are summarized as follows; 1) The strain ratio of the core rubber thread(u) is dependent upon spiral length ratio(${\beta}$). In other words, the strain ratio is dependent upon manufacturing braiding angle($\theta$a), manufacturing drafting ratio(Da) and diameter ratio of covering yarn to core rubber thread ($\phi$). ${\mu}$ shows large values when $\theta$a is decreased to maintain uniformity approaching D=1, and the strain ratio decreases linearly with increasing tan $\theta$a. and the width decreases significantly and the braiding angle decreases linearly, respectively depending upon the drafting ratio of the braid(DB). 3) Results from comparison of the theoretical equations(4) and (8) for width and braiding angle with the experimental values approach the theoretical equation when drafting ratio of core rubber thread(D) and $\theta$a increase. It is anticipated that this fact may be caused by the effect of ${\beta}$. 4) When the structural changes of the elastic braid are considered on the basis of the conception of coefficients of width change(Kw) and braiding angle change(K$\theta$), Kw is dependent upon $\theta$a and K$\theta$ is independent upon both $\theta$a and $\phi$. 5) When the Da is constant, linear relation between K$\theta$ and Kw/D is established and its slope corresponds to Da, nearly coinciding with the experimental curves in Fig. 16. 6) Estimated equations(12) for the manufacturing braiding angle shows optimum conditions within ranges of $\phi$=0.40 to 0.47 and $\theta$a=20$^{\circ}$ to 25$^{\circ}$.

Cell Cycle에 있어서 DNA 합성기는 일정한가?

주강,이융창 대한해부학회 1975 Anatomy & Cell Biology Vol.8 No.2

鷄胎器官 成長에 미치는 Testosterone의 效果에 對하여

朱剛,郭外景,金達洙,朴洋奎 慶北大學校 醫科大學 1967 慶北醫大誌 Vol.8 No.2

Although many research have been described previously for the various effects of testosterone on the several embryos, most of these study are based on simple weight gain which is not adequate for evaluation of the various organs statistically. The author intended to determine the effects of testosterone on the weight gain of body weight and various organs of chick embryos. On the 8th and 12th day of incubation, 0.5㎎ and 7.5㎎ of testosterone were injected into chorioallantoic membrane of each incubated egg of two groups, and each main group were divided into three more small groups such as control, 0.5mg injected group and 7.5㎎ injected group. Between the 12th and 18th of incubation day, a daily total of 10 treated eggs was broken for weighing the body weights of chick embryos and six organs―brain, eye ball, heart, lung, liver and kidney. The effects of doses and difference of injection times on growth were analyzed by the Repeated Two Way Lay out Method, and the determination of the significance in the levels of difference was made by the Turkey's Method. The results are summarized as follows: 1. When injected with testosterone injectable aqueous suspension, the chick embryos were observed with significant difference on the growth of organs between the 8th day group and the 12th day group. 2. In the 8th day group, growth retardation appeared in body weight, brain, eye-ball, lung, liver and kidney throughout 7.5㎎ injected group. 3. Generally, the growth inhibition was found on the 12th day group in comparison with control group. 4. In the both 8th day group and the 12th day group, were unique in that the growth increasing appeared hearts in 7.5㎎ injected group. 5. When injected with 7.5㎎ testosterone on the 8th day, kidney were observed with growth inhibition, but on the other hand, 12th day injected group showed growth promotion.

器官抽出物이 腎發生에 미치는 影響에 對하여

朱剛,李善奎,金達洙,朴洋奎 慶北大學校 醫科大學 1966 慶北醫大誌 Vol.7 No.2

The author expect to know whether the growth of the kidney of chick embryo is influenced by the extracts of some organs. As a test material, 560 eggs of White Leghorn were incubated under the condition of temperature,38±1℃ and relative humidity, 75.0±5%. Of these incubated eggs, only 460 of chick embryos are used as experimental materials. Of these experimental materials, 160 are normal group and rate of incubation, 96%. For experiment, the extracts of brain, kidney and liver of the same species of adult White Leghorn were injected in 36th hour and 72nd hour incubated chick embryo. Random selection of 50 of chick embryo were made from above mentioned 6 group of treatment to measure the weight of kidney and the results were obtained as followings. 1) Inhibition of growth is revealed when extracts of brain, liver and kidney of adult White Leghorn were injected in early chick embryo. (P<0.01) 2) Inhibiting effect on growth of chick embryos is greater by extracts of brain than by extracts of liver and kidney. (P<0.01) 3) Inhibiting action of growth of chick embryos by injection of extracts of brain, liver and kidney is greater in 36th hour incubated chick embryos than 72nd hour incubated chick embryos. (P<0.01)

바터 팽대부에 발생한 선암과 대세포 신경내분비암의 충돌종양 1예

주강,김태효,김차영,이상수,김홍준,김현진,정운태,이옥재 대한소화기내시경학회 2011 Clinical Endoscopy Vol.42 No.2

Most tumors affecting Vater’s ampulla are adenocarcinomas, but a neuroendocrine carcinoma in the ampulla of Vater is extremely rare. The coexistence of these two tumors has been reported in only a few cases. Here, we report a rare case of a collision tumor of the ampulla of Vater with an adenocarcinoma and a large cell neuroendocrine carcinoma. 바터 팽대부에 발생하는 악성 종양은 대부분 선암(adenocarcinoma)이며 다른 조직학적 형태의 암 발생은 극히 드물다. 특히 선암과 신경내분비암이 동시에 발생하는 복합종양은 매우 드문 것으로 알려져 있다. 저자들은 최근 상복부불쾌감과 황달을 주소로 내원한 60세 여자에서 바터 팽대부암 의심 하에 유문보존 췌십이지장절제술 후 조직학적으로 선암과 대세포 신경내분비암이 병발한 바터팽대부의 충돌종양을 진단하였다. 복합종양 중 대세포 신경내분비암이 췌장 주위 지방조직으로의 침윤 및 림프절 전이가 있어 수술 후 보조항암요법을 받았던 예를 경험하여 문헌고찰과 함께 보고한다.

Sump 증후군에 의한 간농양과 위장으로 자발성 농양배액

주강,하창윤,임병렬,신민기,이창민,장현정,민현주,김태효,정운태,이옥재 대한내과학회 2008 대한내과학회 추계학술대회 Vol.2008 No.-

정박아 및 정신분열증환자의 Dermatoglyphics 상의 특징

주강,이인환,손은익 대한해부학회 1978 Anatomy & Cell Biology Vol.11 No.2

Cortisone의 鷄胎器官成長에 미치는 影響에 對하여

朱剛 慶北大學校 醫科大學 1967 慶北醫大誌 Vol.8 No.1

This study is to observe the effects of cortisone on the weight gains of body and various organs of chick embryo, out of which growth and relative growth formula has been derived. On the 8th and the 12th days of incubation, 0.5㎎ and 2.0㎎ of cortisone were injected into the chorioallantoic membrane of each incubated egg of two groups. Between the 12th and 18th of Incubation days, a daily total of 10 treated eggs was broken for weighing the body weights of chicks embryos and six organs-brain, eye ball, heart, lung, liver, and kidney. The effects of doses and difference of injection times on growth were analyzed by the Repeated Two Way Layout Method, and the determination of the significance in the levels of difference was made by the Turkey's Method. The formulas applied to analyzing the changes in growth are: y=a+bt+ct^2, coefficient of relative growth (α) by y=bxα. y= growth quantity of organs; t= specific date of incubation period, x= body weight; a,b,c=constants. The results are summarized as follows: (1) When injected with cortisone acetate, the chick embryos were observed with remarkable growth inhibition(p<0.01). (2) The growth inhibition appeared to be much stronger with the group of 2.0㎎ dosage than that of 0.5㎎ dosage. No noticeable differences were observed on the liver of the 8th day group, and on the kidneys of the 12th day group. (3) Generally, the growth inhibition was found stronger on the 8th day group than on the 12th (p<0.01), but in brain and eye balls, no inhibitory difference depending on the injection days was observed. Lungs were unique in that the growth inhibition appeared in the 12th day group (p<0.01). (4) Growth rate: In the 8th day group, the growth rate was observed to be increased in body weight, lungs, liver, and kidneys throughout the two injected groups- 0.5㎎ and 2.0㎎: brain and heart, however, showed the increase only in 2.0㎎ injected group. The growth rate was observed decreased in eye balls of two injected groups; the decrease of brain and heart were in 0.5㎎ injected groups. In the 12th day group, the increase in growth rate was shown in the followings: body weight, eye balls and lungs of 0.5㎎ injected group, body weight, eye balls and kidneys of 2.0㎎ injected group, the other organs except the increased ones showed decrease in all injected groups. (5) Specific Growth Rate: (a) In the 8th day group: 1) increase was observed only in lungs, 2) decrease was in all organs except lungs. (b) In the 12th day group: 1) increase eye balls of 2.0㎎ injected group; kidneys of 2.0㎎ group. 2) decrease was in the other organs except the above mentioned. (6) The Growth Gradient: The growth centers appeared as follows: (a) In the 8th day group: lungs of all two injected groups, on the 18th day of incubation; liver of 2.0㎎ injected group, on the 15th; kidneys of 0.5㎎ injected group, 17th day of incubation; the others all appeared on the 12th day. (b) In the 12th day group: brain, heart and liver of 0.5㎎ injected group had the growth centers on the 12th incubation day, and the others had them on the 18th incubation day. (7) The Coefficient of Relative Growth: The coefficient of relative growth was observed as follows: (a) In the 8th day group: the liver of two injected group, and kidneys of 0.5㎎ injected group were of positive allometry; the others showed negative. (b) In the 12th day group: liver of two injected groups and heart and kidneys of 2.0㎎ injected group were positive, while the others were negative.