Rhizopus nigricans 로부터 원형질체의 분리

김명희,김말남 ( Myung Hee Kim,Mal Nam Kim ) 한국균학회 1994 韓國菌學會誌 Vol.22 No.2

운동강도와 지속시간에 따른 트레드밀 운동이 비만 쥐의 심장근 내 항산화에 미치는 영향

김명희,김용억,윤창륙,유지원,안종모,Kim, Myung-Hee,Kim, Young-Eok,Yoon, Chang-Lyuk,Ryu, Ji-Won,Ahn, Jong-Mo 대한안면통증구강내과학회 2013 Journal of Oral Medicine and Pain Vol.38 No.1

트레드밀 운동이 비만 쥐의 심장근 내 항산화에 어떠한 영향을 주는지 알아보고자 흰쥐에 6주 동안 고지방 식이로 비만을 유발시킨 뒤 운동강도와 적용시간을 달리하여 트레드밀을 3주 동안 적용하였으며, 체중변화, 혈중지질성분, 조직학적 변화, 심장근 내 항산화 효소와 MCP-1(monocyte chemoattractant protein-1) 등의 변화를 연구하였다. 4주령 Sprague Dawley계 수컷 흰쥐 32마리를 사용하여 각각 일반사료를 섭취한 정상군 (normal), 비만 대조군 (control), 비만 쥐에게 고강도 분할운동을 적용한 실험군 I(Experimental I), 비만 쥐에게 중강도 지구력 운동을 적용한 실험군 II (Experimental II)로 나누었다. 실험 결과 첫째, 체중변화는 고강도 분할 운동을 적용한 실험군 I과 중강도 지구력 운동을 적용한 실험군 II는 대조군에 비해 유의한 감소가 있었다 (p<0.001). 특히, 운동을 적용한 1주에서 3주까지 유의한 차이가 있었다. 둘째, 혈중지질성분 수치는 고강도 분할 운동을 적용한 실험군 I과 중강도 지구력 운동을 적용한 실험군 II는 대조군에 비해 총콜레스테롤, 중성지방, 저밀도 지단백질의 유의한 감소, 고밀도 지단백질에서는 유의한 증가가 있었다 (p<0.001). 셋째, 심장근의 조직학적 소견은 심장근에서 아교질 형성과 평활근 섬유의 정렬 상태와 밀집도, 심장근 세포크기가 중강도 지구력 운동 적용한 실험군 II에서 정상군과 비슷한 형태인 근육들이 잘 밀집해 있는 것을 볼 수 있었다. 또한, 섬유아세포, 염증세포들의 침윤 등이 고지방 식이 유발 쥐인 대조군과 고강도 분할 운동을 적용한 실험군 I에서 가장 심하였으며, 중강도 지구력 운동을 적용한 실험군 II는 대조군에 비해 섬유아세포, 염증세포들의 침윤 등이 현저히 감소하였다. 넷째, 심장근의 항산화 효소 SOD(superoxide dismutase), CAT(catalase), GPx(glutathione peroxidase) 수치는 중강도 지구력 운동을 적용한 실험군 II와 고강도 분할 운동을 적용한 실험군 I에서 모두 유의한 증가가 있었다 (p<0.001). 다섯째, 심장근의 항산화 단백질 MCP-1 발현 수치는 고강도 분할 운동을 적용한 실험군 I과 중강도 지구력 운동을 적용한 실험군 II 모두에서 감소가 있었으며 (p<0.01), 특히, 중강도 지구력 운동을 적용한 실험군 II에서는 유의한 감소가 있었다 (p<0.001). 따라서, 중강도 지구력 운동은 고지방 식이로 유발된 비만 쥐의 심장근 내 항산화에 영향을 주어 비만 개선과 산화적 스트레스에 의한 심장근육 손상 감소 등에 효과적인 것으로 나타났다. The aims of this study was to observed an effect of antioxidative in cardiac muscle of high fat diet induced obesity rat by treadmill exercise with intensity and time. Thirty-two Sprauge-Dawley rats which were divided into four group. Normal, Control(high fat diet induced obesity rat), Experimental I(high intensity intermittent exercise in high fat diet induced obesity rat), Experimental II(moderate intensity endurance exercise in high fat diet induced obesity rat). The results of this study were as follows: 1. In change of body weight, the outcome of each group significantly difference compared with control. Also, 1 to 3 weeks significantly different compared with pre valu experimental I and II(p<0.001). 2. In change of lipid profile, the outcome of each group significantly difference compared with control(p<0.001). Difference between experimental I and II is not significantly. 3. In change of antioxidative enzymes(SOD, CAT, GPx) in myocardium, there are significant difference between control and experimental II, and also between control and experimental I(p<0.001). 4. In change of antioxidative protein MCR-1, the outcome of each group significantly difference compared with control(p<0.01). Experimental II was most significantly difference than the other group(p<0.001). The above results suggest that treadmill exercise effectively reduced in fat. It would be considered that moderate intensity endurance exercise has an effects on improved antioxidative enzyme in cardiac muscle of high fat diet induced obesity rat.

Dehydrogenative Polymerization of Hydrosilanes to Silicon Polymers

김명희,이준,모수용,김종현,우희권,Kim, Myoung-Hee,Lee, Jun,Mo, Soo-Yong,Kim, Jong-Hyun,Woo, Hee-Gweon The Basic Science Institute Chosun University 2010 조선자연과학논문집 Vol.3 No.2

Hydrosilanes possessing reactive Si-H bond are used in synthesizing various types of polysilanes by dehydrocoupling under the influence of various organometallic promoters. Catalytic Si-Si/Si-O coupling of hydrosilanes with hydrosilanes, alcohols, and lactones to silicon-based polymers are described in this article as selective examples of our recent research developments. These silicon-containing polymers can be used as a precursor to prepare useful functional materials for fabricating electronic devices.

뇌혈관 환자에 대한 간호진단 적용 분석

김명희 대구보건대학 1996 대구보건대학 論文集 Vol.16 No.-

간호진단의 실무적용을 증진시키고 간호의 질을 높이기 위해 대구시내에 소재한 Y의료원 신경계 변동에 입원한 환자 102명에게 적용된 간호진단수 총 109개를 NANDA회의에서 채택된 간호진단명 105개에 준하여 분석한 결과 환자 1인당 평균1개를 사용하였으며 총 사용 NANDA 간호진단명은 9개로 대상자 102명중 가장 많이 사용된 간호 진단은 안위의 변화 44.0%, 불안 19.3%, 부적절한 개인적 대응 12.8%로서 높게 나타났다. 본 연구의 결과 적용된 간호진단의 종류 및 빈도가 매우 낮았으며 간호진단에 대한 사정도구개발의 연구와 간호사들에게 지속적인 보수교육이 강화되어야 한다고 생각한다. This research was done for the improvement of practical appliance of Ng. diagnosis and for the elevation of nursing guality(level). I analized the Ng. diagnosis with 109 diagnosis used by NANDA conference from 102 patients who were admitted for Neuro Surgery Ward of Y medical Center which is in Taegu. Ther general characteristics of the patients are as follows ; by sex, men were 57 cases(55.9%) and women were 45 cases(45.1%). In age, 26~29 were I case(1.0%), 30~39 were 10 cases(9.8%), 40~49 were 30 cases(29.4%), 50~59 were 32 cases(31.4%), 60~69 were 26 cases(25.5%), and over 70 were 3 cases (2.9%). In diagnosis of the patients, Cerebral Infarction were 52 cases(50.9%), Intracerebral Hemorrhage were 20 cases(19.6%), Subarachnoid Hemorrhage were 12 cases(11.8%), Epidural Hemorrhage were 7 cases(6.9%), Subdural Hemorrhage were 5 cases(4.9%), Aneurysm were 4 cases(3.9%), Cerebrovascular Accident were 2 cases(2.0%). I used all 109 Ng. diagnoses which means one person used about 1.0 of NANDA'S diagnoses. All used NANDA'S was 9 diagnoses. The most popular diagnoses were alteration in Comfort(44.0%), Anxiety(19,3%), Ineffective individual Coping(12.8%) in 102 cases. Bae showed ineffective air way clearance(48.4%), impaired Physical mobility, ineffective Breathing pattern, Knowledge deficit, All of them were 41.9% in her diagnosis analysis research of Open Heart Surgery Patients. Kang showed self care deficit(27.7%), skin integrity(23.4%), tissue perfusion, alteration in cerebral area(16.4%), and high risk for infection(0.6%), in her diagnosis analysis research of brain disease patients. Park(1987) showed the representative diagnosis of high risk for injury, altered nutrition less than body reguirements, knowledge dificit, sleep pattern disturbance, and fluid volume excess, in her evaluations, of the original and revealational research rate of five diagnosis in patients with CAAH or CAVH. When we consider the specifics of each patient we can determine how the Ng assessment reveals that patients problems differ according to their disease. Signs and symptoms are one thing, and nursing requests are another. As the specific response-formal analysis of 9 Ng. Diagnoses in which the most popular diagnoses were Emotion(33.4%), Exchanging(22.2%), Moving(12.0%), choosing(11.1%) Knowing(11.1%) but Communicating and Relating, Valuing, Perceiving were not applied.

이하선에서 발견된 MALT 림프종 1례

김명희,최종중,안홍근,박중수,김연수,Kim, Myeong Hee,Choi, Jong Joong,Ahn, Hong Geun,Park, Joong Su,Kim, Yeon Soo 대한두경부종양학회 2019 대한두경부 종양학회지 Vol.35 No.1

Mucosa-associated lymphoid tissue (MALT) lymphoma has specific clinical and pathologic features. The most common site MALT lymphomas is the stomach; however, it can also occur in other organs, such as the salivary glands. MALT lymphoma is rare, but its prognosis is good. A 32-year-old man visited Konyang university hospital with parotid mass. Superficial partial parotidectomy was performed to exclude lymphoid neoplasms. IgH gene rearrangement analysis of the surgical specimen led to the diagnosis of MALT lymphoma. The patient underwent esophagogastroduodenoscopy, positron emission tomography-computed tomography, and whole-body bone scan. Regional or distant metastasis was not observed on staging workup. The patient underwent postoperative radiation therapy, there has been no recurrence of MALT lymphoma to date. Here, we report this rare case of parotid MALT lymphoma that was treated with surgery and postoperative radiation therapy.

림프절 전이가 동반된 진행성 위암 환자에서 수술 전 항암요법으로 시행한 FOLFOX 치료로 완전 관해를 보인 1례

김명희,정현용,문희석,성재규,강선형,김주석,Myung Hee Kim,Hyun Yong Jeong,Hee Seok Moon,Jae Kyu Sung,Sun Hyun Kang,Ju Seok Kim 대한소화기암연구학회 2018 Journal of digestive cancer reports Vol.6 No.1

국소적인 진행성 위암의 근치적인 치료는 수술이 첫 번째 방법이다. 하지만 위암을 진단받은 환자 중에서 약 30% 만이 수술이 가능한 진행성 위암으로 진단을 받으며 그 중 약 40-60% 만이 R0 절제술이 가능하다. 저자들은 위 주위 림프절과 복강 림프절, 장막을 침범한 진행성 위암을 진단받은 환자를 3주간의 수술 전 항암화학요법으로 치료하였고, 근치적인 목적으로 위 절제술을 시행하였다. 조직병리검사에서 완전히 괴사된 종양 조직을 관찰할 수 있었고, 수술 후 현재까지 3차례의 추가적인 전신 항암화학요법을 시행하고 있으며, 추적 복부 전산화단층촬영에서 재발을 시사하는 소견을 보이지 않았다.

Bacillus brevis CD162 Cyclodextrin Glycosyltransferase의 정제 및 특성

김명희,임영희,오태광,손천배,Kim, Myung-Hee,Lim, Young-Hee,Oh, Tae-Kwang,Sohn, Cheon-Bae 한국응용생명화학회 1997 Applied Biological Chemistry (Appl Biol Chem) Vol.44 No.4

The cyclodextrin glycosyltransferase (CGTase, EC 3.2.1.19) from Bacillus brevis CD162 was purified by precipitating with ammonium sulfate, DEAE-Sepharose CL-6B column chromatography and Sephadex G-150 column chromatography. The molecular mass and pI of the purified enzyme were estimated to be 74,000 and 6.3 by SDS-PAGE and isoelectric focusing, respectively. The purified enzyme was clearly identified as the CGTase by zymogram after SDS-PAGE. The optimum pH and temperature for the enzyme activity were 8.0 and $55^{\circ}C$, respectively. The enzyme was stable at the range of pH $5.5{\sim}9.0$, and up to $50^{\circ}C$. The amino acid sequence from the $NH_2-terminal$ of the purified CGTase was Ser-Val-Thr-Asn-Lys-Val-Asn-Tyr-Ser-Lys-Asp-Val-Ile-Tyr-Gln. The yields of the products from starch as the substrate were 1.3% for ${\alpha}-$, 33.9% for ${\beta}-$, and 9.7% for ${\gamma}-cyclodextrin$. Bacillus brevis CD162가 생산하는 cyclodextrin glycosyltransferase (CGTase)를 ammonium sulfate 침전, DEAE-Sephadex CL-6B 및 Sephadex G-150 컬럼 크로마토그래피를 수행하여 분리정제하였다. 정제된 CGTase는 분자량이 약 74,000, 등전점은 약 6.3인 단백질이었고, 정제된 단백질을 SDS-PAGE한 후 변성된 단백질을 재활성시켜 zymogram을 수행한 결과 cyclodextrin glycosyltransferase임을 확인할 수 있었다. 이 효소의 최적활성 pH와 온도는 각각 8.0과 $55^{\circ}C$이었으며, pH $5.5{\sim}9.0$과 $50^{\circ}C$까지 안정한 활성을 보였다. 또한, CGTase의 $NH_2$-말단 부위의 아미노산서열은 Ser-Val-Thr-Asn-Lys-Val-Asn-Tyr-Ser-Lys-Asp-Val-Ile-Tyr-Gln 이었으며, 전분으로부터 cyclodextrin으로의 전환률을 분석한 결과, ${\alpha}$-cyclodextrin은 1.3%, ${\beta}$-cyclodextrin은 33.9% ${\gamma}$-cyclodextrin은 9.7% 이었다.

돼지감자로부터 invertase 의 정제 및 특성 연구

김명희,전덕영 ( Myung Hee Kim,Deok Young Jhon ) 생화학분자생물학회 1988 BMB Reports Vol.21 No.4

The invertase (EC 3.2.1.26), which catalyzes the hydrolysis of sucrose, has been purified 71-fold from Jerusalem artichoke tuber. Electrophoresis showed that the native enzyme is consisted of single polypeptide chain of 86,000 daltons. Optimum pH and temperature for the enzyme action were pH 7.0 and 40℃, respectively. The enzyme exhibited high hydrolytic activity to sucrose, but no activity on inulin. The enzyme had an apparent Km of 24.7 mM for sucrose.

담수어중의 (淡水魚中) 총수은함량에 (總水銀含量) 관한 연구 (1) - 수종의 (數種) 금강하류 (錦江下流) 담수어에 (淡水魚) 대하여 -

김명희,박성배 ( Myung Hee Kim,Sung Bae Park ) 한국하천호수학회 1981 생태와 환경 Vol.14 No.3·4

This study was performed to investigate the present contamination level of total mercury in freshwater fishes. The samples were collected at kangkyung area located in Kum river from May to July in 1890. Experimental subjects were fifty seven case from seven kinds of fishes, and dissected into four parts; muscle, bone, liver and gill. Contents of total mercury in the parts were determined by mercury analyzer. The results were as follows: 1. In the muscle tissue, mean Contents of total mercury was 0.351ppm; the highest was 0.522±0.038ppm in shake head; the lowest was 0.207±0.022ppm in eel. 2. The order of the mean value of total mercury contents in four parts were muscle>liver>bone>gill, respectively. 3. Food chain was a factor which affected the concentration of total mercury in the fishes. In the most case, carnivorous fish had higher level of total mercury than omnivorous. 4. In the relationship between size and mercury concentration, mercury level tended to increase with size, age.

입원환자의 의료보장형태에 따른 가정간호 이용의사에 대한 연구

김명희,조은지,박형숙,강인순,Kim, Myung-Hee,Cho, Eun-Ji,Park, Hyoung-Sook,Kang, In-Soon 한국가정간호학회 2005 가정간호학회지 Vol.12 No.2

Purpose: This study is a descriptive research which is designed to investigate hospitalized patients' intent to use home care nursing according to the types of medical security. Method: This researcher surveyed 236 patients who were hospitalized at B medical center located in Busan,. Data were collected from Sep. 1 to Nov. 30, 2005 using a questionnaire survey, medical records, face-to-face interviews and observations. Collected data were analyzed in terms of frequency, percentage, mean and standard deviation through $x^2$-test and t-test under SPSS WIN 10.0 Program. Result: Out of the total subjects, 59.3% were medical aid clients and the remaining 40.7%, health insurance ones. The hospitalized period and frequency of the former group were 38.0 days and 4.0 times, respectively, while those of the latter, 37.7 and 3.4. When home care nursing clients were examined using a given classification device, it was found that out of the total 236 subjects, 205(86.9%) were needed to receive home care nursing, 121, medical aid and the other 84, health insurance. 24.0% of medical aid clients heard about home care nursing ever before, lower than 39.3% of health insurance clients. 43.8% of the former clients said cost for home care nursing was high while, 47.6% of the latter group responded expense for the nursing intervention was low. 30.6% of medical aid clients had intent to use home care nursing, lower than 47.6% of health insurance clients. 71.7% of those patients whose monthly income was 99 million won or below had no intent to use home care nursing, higher than 62.5% of those who were 100 million or over in monthly income(p<.05). 76.4% of those clients who had no nursing provider intented to use home care nursing, higher than those who had nursing provider(p<.05). Concerning contents of home care nursing, 85.1% of medical aid clients needed education, training and counseling while, 77.4% of health insurance aids wanted medication and injection. Conclusion: In conclusion, the use of home care nursing by medical aid clients should be promoted through improving conditions for home care nursing in terms of expense, family and residence and making public relations about activities and contents of the home care nursing.