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소화효소가 Helicobacter pylori의 증식과 공포화독소 생성에 미치는 영향
홍대식,강경희,장명웅 고신대학교(의대) 고신대학교 의과대학 학술지 2001 고신대학교 의과대학 학술지 Vol.16 No.1
Background : Global high prevalence rate of Helicobacter pylori infection is well-known fact. However, a large discrepancies exist between the diagnostic data obtained by the PCR and culture method, because the coccoid form of H.pylori which represent the majority form in the biological samples are not viable in the standard BHI broth with 5% horse serum. The component parts of saliva and gastric juice acted as neither stimulator nor inhibitor to the growth of H.pylori remain unclear. Objective : The purpose of this study was to evaluate the influence of amylase, pepsin, mucin, and lysozyme on the growth and vacuolating toxin production and titer of H.pylori in vitro. Materials and Methods : H.pylori 13,39,46,59,72,94,98 and 125 strains were isolated from patients with gastric disease. The digestive enzymes were inoculated in the 5% horse serum-BHI broth following final concentration of amylase (A; 0.20ug/ml), pepsin (P; 0.25ug/ml), mucin (M; 2,5ug/ml), and lysozyme (L; 1.5ug/ml). The vacuolating toxin titers of the culture supernatant were evaluated in the monolayer culture of RK-13 cells. Results : The morphologic conversion from bacillary to horse-shoe to coccoid forms of H.pylori was observed in the BHI broth containing 5% horse serum on day 5 culture. Half of H.pylori was changed from bacillary to coccoidal form on day 7 culture, most population of H.pylori was changed to coccoidal form on day 10 culture, and almost all H.pylori was changed to coccoidal form on day 14 culture. The growth of H.pylori 72, and 94 strains was increased in BHI broth containing each enzyme such as amylase, pepsin, mucin and mixture of two or three enzymes on day 5 and 7 culture. The growth of H.pylori 94 strains was increased in BHI broth containing each enzyme such as amylase, pepsin, mucin, and mixture of two or three enzymes on day 10 and 14 culture. The vacuolating toxin production of H.pylori strains was not influenced by the addition of amylase, pepsin, mucin, and lysozye or mixture of two or three enzymes. The neutralization of vacuolating toxin of H.pylori was not affected by the addition of amylase, pepsin, mucin, and lysozyme or mixture of two or three enzymes. Conclusion : We found that coccoid forms of H.pylori were viable in the standard 5% horse serum BHI media in the presence of amylase, pepsin, mucin, and lysozyme. The present study demonstrates that gastric juice of the stomach supports the viability of H.pylori and that oral route of infection is a plausible hypothesis. This work also demonstrates that gastric environment may not provide a selective advantage for the toxigenic strains of H.pylori.
홍대기(Hong, Dae-Ki),조태경(Cho Tae-kyung),박병수(Park Byoung-soo) 한국산학기술학회 2008 한국산학기술학회논문지 Vol.9 No.5
최근 국내대학 전반에 걸쳐서 공과대학의 우수 지원자가 점차로 감소되고 있는 상황이며 고등학교 수학 교육과정의 부실화에 따른 신입생의 학력저하에 대한 우려 또한 제기되고 있다. 이러한 위기상황에 대응하여 상명대학교에서는 공학교육의 발전 및 혁신을 위해 공학교육 혁신센터를 운영하고 있다. 공학교육 혁신센터는 공학교육 혁신을 위한 장가계획의 일환으로 수학 분야의 교육 강화를 위한 여러 가지 방안을 논의하여 노출하고 있다. 본 논문에서는 상명대학교에서 논의하여 도출한 수학 교육 강화방안을 제시한다. 이러한 결과들은 상명대학교 공과대학과 유사한 환경에 처해있는 다른 학교들의 수학 교육 강화를 위한 참고자료로 활용될 수 있을 것이다. Recently, the number of applicants for the engineering college is gradually decreased all over the nation. Additionally, the concerns of fall of freshman mathematical ability are raised due to the incomplete high school education. Therefore, the local universities endeavor in order to improve the quality of mathematics education, and study the countermeasure against the crisis. As part of these efforts, Sang-Myung University founds the innovation center for engineering education. The center derives several ideas in order to enhance the mathematics education quality. In this paper, the enhancement plans for the mathematics education in Sang-Myung University are proposed. Results in this paper will be helpful for other university to enhance the mathematics education as reference materials.
홍대영,백광제,이경룡,권운용,박준석,어은경,오범진,이미진,이성우,서주현,노형근,김준식,Hong, Dae-Young,Baek, Kwang-Je,Lee, Kyeong-Ryong,Kwon, Woon-Yong,Park, Joon-Seok,Eo, Eun-Kyung,Oh, Bum-Jin,Lee, Mi-Jin,Lee, Sung-Woo,Suh, Joo-Hyun,Roh, 대한임상독성학회 2007 대한임상독성학회지 Vol.5 No.1
Purpose: Owing to organophosphate-pyrethroid mixtures are widely used, suicidal or unintentional poisoning is common. But there have been relatively few reports of poisoning. The purpose of this study is to evaluate the difference of the severity and toxicity between organophosphate-pyrethroid mixtures poisoning and single organophosphate poisoning. Methods: From August 2005 to July 2006, 65 patients presented with organophosphate-pyrethroid mixtures poisoning to emergency medical center. Date were gathered by report form it was drawn up. Results: 65 patients were enrolled in 28 hospitals and their mean age was $56.5{\pm}16.2$ years old. The most common cause of poisoning was suicide, in 52 cases(80%). Chlorpyrifos-cypermethrin, malathion-esfenvalerate were the most frequent chemicals involved, and the mean ingestion amount was 135.4ml. The most common symptom of the patients was nausea/vomiting, in 16 patients. The average GCS score was 13. The mean ICU stay was $4.4{\pm}5.2$ days, and mortality was 3.1%. Conclusion: The severity and toxicity of organophosphate-pyrethroid mixture poisoning were lower than that of single organophosphate poisoning.