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PCR을 이용한 해외 유입 설사질환 원인 세균성 병원체의 분포 및 특성 연구
전세미,김준영,이하림,손민영,박미선,이복권,김성한 대한임상미생물학회 2009 Annals of clinical microbiology Vol.12 No.4
Background: The incidence of infectious diarrheal disease in Korea has decreased over the past decade, but traveler's diarrhea (TD) is increasing in frequency. We therefore investigated the distribution of the causative agents of TD. Methods: A total of 132 rectal swab specimens were acquired from TD patients who entered the country via Gimhae International Airport. The specimens were screened for 12 bacterial pathogens by real- time PCR, and target pathogens were isolated from the PCR positive specimens using conventional microbiological isolation methods. Results: A total of 93 specimens (70.5%) showed positive PCR screening results, and of these specimens, nine species and 50 isolates (37.9%), including Vibrio parahaemolyticus (18 isolates) and ETEC (17 isolates), were isolated. No specimens were PCR positive for Listeria monocytogenes or Campylobacter jejuni, and no pathogenic Bacillus cereus were isolated. Conclusion: Even though viruses and EAEC were not included as target pathogens, the high isolation rate of these pathogens in this study provides indirect evidence that most cases of pathogen-negative TD are caused by undetected bacterial agents. Furthermore, our study results confirm the effectiveness of real-time PCR-based screening methods. This study is the first report in Korea to demonstrate that ETEC and V. parahaemolyticus are the major causative pathogens of TD, and this knowledge can be used to help treat and prevent TD.
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전세경(Jeon Se Kyung),박정윤(Park Jung Yoon),김유정(Kim Yu Jeong) 한국실과교육학회 2000 한국실과교육학회지 Vol.13 No.3
This study has analyzed how the teacher education program is constructed and managed to promote the prospective elementary teachers’ expertise on Practical Arts Education in National University of Education. Especially, the research was focused on whether and how the students in National University of Education have been appropriately learning the content knowledges and teaching methods for each domain of Elementary Practical Arts through the Practical Arts Education course. 282 senior students enrolled nationwidely in National University of Education were employed as research subjects and a questionnaire was administered to them just before the graduation for the present study. The study results were analyzed by finding the frequencies, means, and percentiles. The research findings have shown that (1) the study of content knowledge in each domain of Elementary Practical Arts was not covered in balanced fashion, (2) most Practical Arts Education courses have dealt dominantly with only theories without vivid experiment in all domains of Elementary Practical Arts except computer domain, and (3) the teaching methods for Elementary Practical Arts were mostly neglected.
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