Development of a noninvasive KIM-1-based live-imaging technique in the context of a drug-induced kidney-injury mouse model

Tae-Jun Kwon,Da-Sol Lee,Md. Enamul Haque,Rang-Woon Park,Byungheon Lee,Dongkyu Kim,Yong-Hyun Jeon,Kil-Soo Kim,Sang Kyoon Kim 한국실험동물학회 2021 한국실험동물학회 학술발표대회 논문집 Vol.2021 No.7

The development of reliable methods to diagnose acute kidney injury is essential to allow the adoption of early therapeutic interventions and evaluate their effectiveness. Based on the fact that kidney injury molecule-1 (KIM-1) expression levels in kidneys are markedly upregulated early after a damage event, here we developed a noninvasive KIM- 1-based molecular imaging technique to detect kidney injury. First, we took advantage of a phage-display platform to select small peptides demonstrating a specific high binding affinity to KIM-1. The promising candidate was conjugated with fluorescent probes, and its imaging potential was validated in vitro and in vivo. This peptide, with the sequence CNRRRA, not only showed a high imaging potential in vitro, allowing a strong detection of KIM-1 expressing cells by microscopy and flow cytometry but also generated a strong kidney-specific signal in live-imaging in vivo experiments in the context of a drug-induced kidney-injury mouse model. Our data overall suggest that the CNRRRA peptide is a promising probe to use in the context of in vivo imaging for the detection of KIM-1 overexpression in damaged kidneys.

김정은 체제 외교정책변화의 결정요인 분석: 대북제재와 국내정치 동학

조현용 ( Jo Hyun-yong ),정기원 ( Jung Key-one ),김용호 ( Kim Yong-ho ) 국방대학교 안보문제연구소 2019 국방연구 Vol.62 No.4

이 글은 2018년 정상회담을 계기로 나타난 김정은 외교정책 변화의 결정요인을 분석하고 있다. 보다 구체적으로 북한 외교정책의 변화를 대내외 변화에 대한 정치적 동학을 통해 규명함으로써 북한의 대내정치 과정에서 파생되는 정치변수들에 대한 분석 역시 한반도 비핵화를 둘러싼 국제정치적 동학 못지않게 중요한 작용을 하고 있음을 주장하고 있다. 독재체제의 정권유지와 경제발전에 대한 정치경제이론을 적용하여 이념 중립적 시각에서 정책변화의 동인을 추적하였으며 기존연구들이 간과해 온 국제환경과 국내정치 간의 상호작용 동학에 의한 변화라는 대안적 분석틀을 제시했다. 이 과정을 통해 도출된 외교정책변화의 핵심변수는 대외요인으로 ‘자원권력’, 국내정치요인으로 지도자의 ‘정치적 생존’과 ‘정치체제’로 나타났다. 김정은 정권의 외교정책변화는 경제제재에 의한 북중무역의 붕괴로 촉발된 것으로 분석되며, 내부적으로 지배연합에 대한 통제력 강화, 군부의 정치 영향력 축소, 제도적으로 당-국가체제 확립을 통한 국내정치적 필요라는 변수가 작용한 것으로 분석됐다. The article analyzes the determinants of North Korean foreign policy in the wake of the 2018 summit. The paper argues that his new foreign policy initiative was motivated by his perceived shortage in his co-optation resources resulted from Chinese active participation on international sanction. In particular, Chinese participation in the ban of coal export triggered his policy changes because North Korea’s export of coal has served as the key source of North Korea’s co-optation resource to maintain Kim’s political survival. In so doing, the paper focuses on the interaction between international environment and domestic politics, which previous studies have overlooked. This paper also identifies key variables of North Korea’s foreign policy changes, “resource power” as external factors, and the leader's “political survival” as domestic variable.

위암 환자의 복강내 투여를 위한 Activated Charcoal-Alginate Bead 제형으로부터 Mitomycin C의 용출 거동

이진호,최선웅,서중기,김동민,정경수,오정연,김진향,노승무,민병무,김용백,김창식,박근성,강대영,송규상,양준묵,조준식,정현용,김학용,인현빈 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

Locoregional recurrence is the most common type of recurrence in surgical operation of gastric adenocarcinoma, and peritoneal dissemination is one of the most difficult problems in advanced gastric adenocarcinoma treatment. Because the peritoneal cavity is the most common site of the first recurrence after gastric cancer resection, intraperitoneal chemotherapy seems a logical choice for cancer chemotherapy. In this study, Mitomycin C (MMC)-activated charcoal (CH)-alginate (ALG) beads were prepared by the mixtures of CH particles adsorbed with MMC as an anti-cancer drug and aqueous alginate solution. The alginate is recognized as biodegradable, nontoxic, and biocompatible. The release of MMC from the beads in 0.1 M Tris buffer was stable and continuous until about 1 week. The MMC-CH-ALG beads can be applied in the peritoneal cavity for intraperitoneal chemotherapy since they provide a good adhesiveness on the tissue and controlled release pattern of the drugs.

위암환자의 복강내에 투여한 Mitomycin C-Carbon Particle의 Mitomycin 용출에 관한 연구

노승무,조영훈,정경수,오정연,김진향,양준묵,강대영,송규상,조준식,최선웅,이진호,민병무,김용백,김창식,박근성,인현빈,정현용,김학용 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

Locoregional recurrence is the most common type of recurrence in surgical operation of gastric adenocarcinoma, and peritoneal dissemination is one of the most difficult problems in advanced gastric adenocarcinoma treatment. Because the peritoneal cavity is the most common site of the first recurrence after gastric cancer resection, intraperitoneal chemotherpy seems a logical choice for cancer chemotherapy. The Mitomycin C(MMC) adsorbed by the activated charcoal particles(CH) is relatively released when the drug concentration surrounding the carbon particles becomes low in the peritoneum of the peritoneal cavity. For the intraperitoneal chemotherapy on the advanced gastric adenocarcnoma, mitomycin C adsorbed on activated carbon particles was administered in the peritoneal cavity just before abdominal wall closure. The closed drainage tubes were inserted in the peritoneal cavity and clamped for tuo hours after completion of operation. MMC concentrations were serially measured in peritoneal fluid, plasma and urine at 2hour, 48 hour, 72 hour and 168 hour following its administration in order to study the efficacy of the MMC-CH as a drug delivery system. There were minimal toxicities in born marrow, liver, and gastrointestinal system after intraperitoneal MMC-CH administration. The data of this study suggested that MMC-CH may have a somewhat more beneficial effect than surgery alone when administered in optimal dose and schedules, but the MMC concentration of the peritoneal fluid was not sufficient to eradicate remnant cancer cells, and effective duration of maintenance was only below 24 hours in the peritoneal fluid and plasma.

강변여과에서 파일럿규모 수평집수관의 수두분포 연구

정재민(Jae Min Jeong),박재영(Jae Young Park),이종진(Jong Jin Lee),김용운(Yong Woon Kim),김승현(Seung Hyun Kim) 大韓環境工學會 2013 대한환경공학회지 Vol.35 No.5

강변여과용 수평집수관을 모사하는 파일럿 규모의 모래통 실험을 수행하였으며, 다양한 출구유속에 대한 수평집수관에서의 수두분포를 구하였다. Kim24)이 수행한 실험실 규모의 수평집수관에서의 손실수두에 Kim25)이 개발한 이론 즉, 작은 규모의 수평집수관에서의 손실수두로부터 큰 집수관의 손실수두를 계산하는 방법을 적용하여 본 연구의 관측치와 비교함으로써 Kim25)의 이론이 타당함을 알 수 있었다. 또한, 이들의 이론을 적용하여 본 연구에서의 관측치로부터 실규모 집수관에서의 수두분포를 구하는 방안을 예를 들어 설명함으로써 강변여과의 수평집수관 설계방법을 제시하였다. A pilot-scale sand-box experiment simulating a lateral of collector well used in riverbank filtration was performed, and hydraulic head distributions along the lateral were measured according to the various drawdowns at the well. The results of this experiment were compared to the predicted values obtained using a theory previously developed by Kim25) in order for its validation. This theory predicts the head loss in a large-scale lateral given the loss in a small-scale one, and to have actual values for comparison, the results from a lab-scale experiment previously performed by Kim24) were employed. Comparing the experimental values to the extrapolated value indeed confirmed the validity of the theory. A procedure to determine the hydraulic head distribution of a practical-scale lateral was also presented applying the theory to the experimental result of this study in an effort to show the process of lateral design for riverbank filtration.

경희대학교 평화의 전당 노래비 디자인

김동찬,김호겸,민창기,김태현,김용욱 경희대학교 디자인연구원 2003 예술· 디자인학연구 Vol.6 No.1

The purpose of this study is to design a basic draft of a monumental inscription with a poem of a peace for stirring up a peaceful mind. The inscription is expected to be a place for arousing a peaceful mind to visitors. Moreover, this place can be a place for culture and rest, which uplifts the image and phase of Kyunghee University to both.

아세클로페낙 연질캡슬(클란자 에스 연질캡슬)의 개발

용철순,이경희,최진석,박병주,정세현,김용일,박상만,배명수,김귀자,김영식,유창훈,강성룡,유봉규,이종달,최한곤 한국약제학회 2004 Journal of Pharmaceutical Investigation Vol.34 No.1

To develop and aceclofenac soft capsule, four preparations with various solubilizers were prepared and their dissolution test was carried out. Among four preparations tested, a preparation with ethanolamine was selected a formula of aceclofenac soft capsule (Clanza S^(™), since it showed the fastest dissolution rate. Bioequivalence of aceclofenac tablet, Airtal^(™)(Dae-Woong Pharmaceutical Co., Ltd.) and aceclofenac soft capsule, Clanza S^(™)(Korea United Pharmaceutical Co., Ltd.) was evaluated according to the guideline of KFDA. Fourteen normal male volunteers (age 20-25 years old) were divided into two groups and a randomized 2×2 cross-over study was employed. After oral administration of one tablet or capsule containing 100 ㎎ of aceclofenac, blood ws taken at predetermined time intervals and the concentration of aceclofenac in plasma wa determined with an HPLC method under UV detector. The pharmacokinetic parameters (C_(max), T_(max) and AUC_(t)) were calculated and ANOVA was utilized for the statistical analysis of parameters using logarithmically transformed AUC_(t), C_(max) and T_(max) between Airtal tablet and Clanza soft capsule were 2.89%, 0.18% and 43.0%, respectively. There were no sequence effects between two formulations in these parameters. The 90% confidence intervals using logarithmically transformed data were within the acceptance range of log(0.8) to log(1.250(e.g.log(0.81) - log(1.23) and log(0.89) - log(1.14)) for AUC_(t) and C_(max), respectively. Thus, the criteria of the KDFA guidelines for the equivalence was satisfied, indicating that Clanza S^(™) soft capsule is bioequivalent to Airtal^(™) tablet.

유도 '되치기 본'의 개선 방안에 관한 연구

김의환,김도준,김규수,김관현,김종달,최종삼,조용철,박순진,윤익선,안병근,정 훈,김미정,한성철 龍仁大學校 武道硏究所 1999 武道硏究所誌 Vol.10 No.1

The purpose of this study was to reform practically a Korean Judo's Doechigi-Bon(Forms of Counterattack, judo's Gaeshi no Kata, Judo's Uradori no Kata, Judo's Gonosen no Kata) that was established in 1955 Korea, according to changing of techniques by Judo's modernization, in order to have Judo's carefulness and systematic diffusion. Reform procedure of Judo's Doechigi-Bon was 1st stage, Questionnaire survey 303 judokas, 2nd stage, Technical seminar by judo experts(12 judo professor) 4 times, 3rd stage, wording report for reform, 4rd stage, Discussion and judgement of Teaching and Judgement commission of Korean judo Association(KJA), 5th stage, Public hearing for reform in KJA, 6th stage, Report and decision of board of directors in KJA, 7th stage, public publication of Judo News(No.53) in KJA. Basic principle of reform of Judo's Doechigi-Bon were as table 1. Table 1. Basic principle of reform of Judo's Doechigi-Bon ------------------------------------------------------------------------------- Items Reformed Key Points of Judo's Doechigi-Bon ------------------------------------------------------------------------------- 1. Structure 1) Grand classification : Classified by 3 parts(1,2,3Gyo) 2)Medium classification : Te waza, Goshi waza, Ashi waza devided per each part(Gyo) 3)Sub-classification : Classified five techniques per each part(Gyo) 2. Contents 1) Selected established techniques as possible 2) Considered rationalty and overlapping of counterattack techniques 3. Decision of Conterattack techniques 1) Refered to results of Basic Questionnaire survey 2) Priority to decisions of Judo expert technical seminar -------------------------------------------------------------------------------- Reformed Korean Judo's Doechigi-Bon(Forms of Counterattack techniques-KJA, 1999) are as follows: 1. Gyo; ① Uki otoshi -> Uchi mata ② Harai goshi -> Harai goshi gaeshi ③ O soto gari -> O soto gake ④ Ko uchi gari -> Sasae tsurikomi ashi ⑤ O uchi gari -> Ko soto gari 2. Gyo; ① Ippon seoi nage -> Okuri eri jime ② Tsuri domi goshi -> Uki waza ③ Okuri ashi harai -> Okuri ashi harai ④ Ko soto gari -> Tai otoshi ⑤ Hiza guruma -> Hiza guruma 3. Gyo; ① Kata guruma -> Sumi gaeshi ② Tai otoshi -> Ko soto gari ③ Hane goshi -> Harai tsurikomi ashi ④ Uchi mata -> Tai otoshi ⑤ Tomoe nage -> O uchi gari

자기효능감과 수업동기 및 학교생활적응간의 관계 탐색

김용래,유효현 弘益大學校 人文科學硏究所 2002 人文科學 Vol.10 No.-

The purpose of this examination was to find out the relationship among secondary school learners' self-efficacy, instructional motivation and school life-related coping. And in order to achieve the purpose of this examination sampled middle/high school learners in Seoul and other areas. The instruments used for the data collection were 「Self-efficacy Scale」 (Kim, A-Young, 1997), 「School Learning Motivation」 (Kim, Yong-Rae, 2000) and 「School Adjustment Scale」 (Kim, Yong-Rae, 2000). The collected data analysis were performed using SPSS/WIN(11.0V) program, and analysis Mean score(M), Standard Deviation(SD), Zero Order Correlation(r) and Multi-Regression Analysis All statistic values were tested by t-test and F-test in the significant level of p<05. And as for the major findings of this examination, 'self-efficacy', 'instructional motivation' and 'school life-related coping' were significantly positive correlation. And 'self-efficacy' and 'instructional motivation' were significantly positive influences on Korea secondary school learners' 'school life-related coping' respectively.

다단계 임계치를 이용한 패턴 디지털 워터마킹

김현환,김용민,김두영 東亞大學校 附設 情報通信硏究所 1999 情報通信硏究所論文誌 Vol.7 No.1

Digital Watermarking is a research area which aims at hiding secret information in digital multimedia contents such as image, audio, and video. In this paper, we propose a new watermaking method with visually recongnizable pattern into the digital image using wavelet transform, spread spectrum method and multilevel threshold value in considering the wavelet coefficients. The watermark information can be extracted by subtracting wavelet coefficients between the origitnal image and orginal image and the watermarked image. The esperimental results show that the prosposed technique is invisible watermarking and robust to JPEG lossy compression, resizing, masking, and filtering.