Effects of salinity on bacterial communities, Maillard reactions, isoflavone composition, antioxidation and antiproliferation in Korean fermented soybean paste (<i>doenjang</i>)

Kim, Mi Jeong,Kwak, Han Sub,Kim, Sang Sook Elsevier 2018 Food chemistry Vol.245 No.-

<P><B>Abstract</B></P> <P>The purpose of this study was to investigate bacterial communities and health-benefit-related parameters in <I>doenjang</I> prepared with various brine concentrations (8, 12, 16, and 20%). Phenolic, flavonoid, melanoidin and isoflavone contents, antioxidation, and antiproliferation of <I>doenjang</I> (AD8, AD12, AD16, and AD20) aged for 3 months were compared with those of initial <I>doenjang</I> (ID8, ID12, ID16, and ID20). The ID8 and AD8 <I>doenjang</I>, made with 8% brine, contained higher phenolics, melanoidins, and isoflavones than those with high salinity. As results of bacterial communities, <I>Enterococcus</I> was a dominant bacterium in most <I>doenjang</I>, while <I>Lactobacillus</I> was predominant in AD8. Trolox equivalent antioxidant capacity (32.5 µmol TE/g dwb) and DPPH radical-scavenging capacity (57 µmol TE/g dwb) values of AD8 were the highest among samples. The <I>EC</I> <SUB>50</SUB> of HT-29 cell proliferation treated with AD8 was 0.47 mg/mL, while <I>EC</I> <SUB>50</SUB> of AD20 was 1.07 mg/mL, indicating stronger antiproliferative activity in low-salinity <I>doenjang</I>.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Salinity of <I>doenjang</I> affected by bacterial communities. </LI> <LI> <I>Lactobacillus</I> was a dominant bacterium in salt-reduced <I>doenjang</I>. </LI> <LI> Salt-reduced <I>doenjang</I> contained more daidzein and genistein than others. </LI> <LI> Salt-reduced <I>doenjang</I> was relatively high in antioxidation and antiproliferation. </LI> </UL> </P>

마늘껍질 70% 에탄올 추출물의 인간 암세포 증식억제 활성

손대열 한국식품저장유통학회 2017 한국식품저장유통학회지 Vol.24 No.2

Ethanol extract of garlic peels (GPE) was investigated for its antiproliferative effects on human cancer cell lines. Human lung cancer cell line A549 treated with 500 μg/mL GPE resulted in the growth inhibition of A549 by 90%. In stomach cancer cell AGS proliferation inhibition activity, GPE showed 45% and 71% inhibition of AGS growth at 1,000 μg/mL and 2,000 μg/mL, respectively. GPE inhibited the growth of the breast cancer cells MCF-7 effectively at low concentration and showed 78% and 90% inhibitions of MCF-7 growth at 200 μg/mL and 500 μg/mL , respectively. GPE showed very significant antiproliferation effect on liver cancer cell line Hep3B and inhibited Hep3B cell growth by 57% at 100 μg/mL, and the inhibition’s rate increased up to 87% at 500 μg/mL. Antiproliferation effect of GPE on colorectal cancer cell HT-29 showed 15% reduction of HT-29 cell growth at 200 μg/mL and the growth rate was reduced in a dose dependent manner up to 1,000 μg/mL. These results indicated that GPE had high antiproliferation effects on breast and liver cancer cell lines at low concentrations (200 μg/mL), and by higher concentrations over 500 μg/mL, GPE inhibited the growth of A549 and HT-29. The results of our study suggested the potential use of garlic peels for use as an excellent antiproliferative substance for human cancer cells.

버섯균사체로 발효시킨 인삼 추출물의 암세포 증식억제 효과

김현영(Hyun Young Kim),정은미(Eun Mi Joung),황인국(In Guk Hwang),정재현(Jae Hyun Jeong),유광원(Kwang Won Yu),이준수(Junsoo Lee),정헌상(Heon Sang Jeong) 한국식품영양과학회 2010 한국식품영양과학회지 Vol.39 No.1

상황버섯, 영지버섯 및 노루궁뎅이버섯 균사체로 발효된 인삼추출물이 암세포증식에 미치는 영향을 살펴보기 위하여 위암세포(MKN-45), 대장암세포(HCT116), 유방암세포(MCF-7), 폐암세포(NCIH460), 전립선암세포(PC-3) 및 간암세포(HepG2)에 농도별(0.25~1.5 ㎎/mL)로 처리하여 암세포 성장억제율을 측정하였다. 전립선암 세포에서의 암세포 증식억제 효과는 영지버섯 균사체발효 인삼추출물이 1.5 ㎎/mL 농도에서 3.07%로 가장 낮은 생존율을 나타내었으며, 상황버섯 균사체발효 인삼추출물이 35.05%, 노루궁뎅이 버섯 균사체발효 인삼추출물이 44.29%의 생존율을 보였다. 폐암세포에 대한 세 가지 버섯균사체발효 인삼추출물의 1.5㎎/mL 농도에서 영지버섯 균사체발효 인삼추출물은 5.31%로 우수한 항암활성을 나타낸 반면, 상황버섯 균사체발효 인삼추출물이 53.52%, 노루궁뎅이버섯 균사체발효 인삼추출물이 35.27%의 생존율을 나타내었다. 이러한 결과로부터 영지버섯 균사체로 발효시킨 인삼추출물이 다른 균사체 인삼 발효물보다 다양한 암세포에 대한 성장억제 효과가 우수함을 확인할 수 있었다. This study was conducted to investigate the effects of fermented ginseng extract by mushroom mycelia on antiproliferation of cancer cells. Phellinus linteus, Ganoderma lucidum, and Hericium erinaceum mycelia were inoculated to ginseng. The effects of fermented ginseng extract on antiproliferation of stomach (MKN-45), colon (HCT116), mammary (MCF-7), lung (NCIH460), prostate (PC-3), and liver (HepG2) cancer cells were investigated by MTT assay. Fermented ginseng extract showed significant antiproliferation effects compared with fresh ginseng extract. Fermented ginseng extract by P. linteus, G. lucidum, and H. erinaceum mycelia showed growth-inhibitory effect of 44.50, 17.75 and 43.98% viability at 1.5 ㎎/mL on the MKN-45 cell line, 62.86, 3.73, and 54.55% at 1.5 ㎎/mL on the HCT116 cell line, 41.81, 7.01, and 37.84% at 1.5 ㎎/mL on the MCF-7 cell line, 53.52, 5.31, and 35.27% at 1.5 ㎎/mL on the NCIH460 cell line, 35.05, 3.07, and 44.29% at 1.5 ㎎/mL on the PC-3 cell line, and 59.57, 6.34, and 4.97% at 1.5 ㎎/mL on the HepG2 cell line, respectively. These results indicated that fermented ginseng by G. lucidum mycelium showed the highest antiproliferation effect against various cancer cells.

Effects of curcumin-/boron-basedcompound complexation on antioxidant and antiproliferation activity

김준영,강윤영,김은지,안중훈,목혜정 한국응용생명화학회 2018 Applied Biological Chemistry (Appl Biol Chem) Vol.61 No.4

Simple and reproducible formulation strategies are needed to improve the bio-availability of curcumin. In this study, curcumin was successfully complexed with two boron-based compounds: 2-aminoethyl diphenyl borate (DPBA) and bortezomib (BTZ; Velcade). In reversephase high-performance liquid chromatography, DPBA/ curcumin complexes (DPBA/cur) showed delayed elution times compared to those of free curcumin. The UV–visible absorbance peak of DPBA/cur and BTZ and curcumin complexes (BTZ/cur) appeared redshifted. DPBA complexation has a negligible effect on the antioxidant and antiproliferation properties of curcumin for two types of cancer cells: MCF-7 and A549. Thus, curcumin complexation with boron-based compounds could be a method to enhance in vivo stability without loss of bioactivity (i.e., antioxidant and antiproliferation effects).

Development of iron(II) sulfate nanoparticles produced by hot-melt extrusion and their therapeutic potentials for colon cancer

Koo, Ja Seong,Lee, Song Yi,Azad, Md Obyedul Kalam,Kim, Minju,Hwang, Sung Jun,Nam, Suyeong,Kim, Sungyun,Chae, Byung-Jo,Kang, Wie-Soo,Cho, Hyun-Jong Elsevier 2019 International journal of pharmaceutics Vol.558 No.-

<P><B>Abstract</B></P> <P>Particle size reduction of FeSO<SUB>4</SUB> (iron(II) sulfate, IS) from micron to nano size was achieved by a combination of hot-melt extrusion (HME) processing and the input of Span 80, Tween 80, and poly(ethylene glycol) (PEG) 6000. Conveying, kneading, and extruding steps of the HME process and a decrease in the surface tension by surfactants were introduced to produce FeSO<SUB>4</SUB> nanoparticles (NPs) in an aqueous environment. The FeSO<SUB>4</SUB>-based NPs (ISNPs) in the dispersion were composed of FeSO<SUB>4</SUB>, Span 80, Tween 80, and PEG 6000 and displayed a hydrodynamic size of 350–400 nm (5–50 mg/mL ISNPs concentration range) and a spherical shape. Considering the feeding ratio of FeSO<SUB>4</SUB> (20%, w/w) used for preparing the ISNPs, FeSO<SUB>4</SUB> appears to be wrapped by Span 80, Tween 80, and PEG 6000 according to the results of X-ray photoelectron spectroscopy (XPS) analysis. ISNPs exhibited different thermodynamic properties from those of FeSO<SUB>4</SUB> itself. In colon adenocarcinoma (Caco-2) cells, the ISNPs group exhibited enhanced antiproliferation and apoptosis potentials compared to the FeSO<SUB>4</SUB> group (<I>p</I> < 0.05). Histological staining data of a dissected intestine after oral administration of ISNPs suggest the absence of severe intestinal toxicities compared to the control (no treatment) group. All of these results imply the feasibility of the use of the developed ISNPs for the treatment of colon cancers with oral administration.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Phloretin-loaded fast dissolving nanofibers for the locoregional therapy of oral squamous cell carcinoma

Nam, Suyeong,Lee, Song Yi,Cho, Hyun-Jong Elsevier 2017 JOURNAL OF COLLOID AND INTERFACE SCIENCE - Vol.508 No.-

<P><B>Abstract</B></P> <P>Fast dissolving nanofiber (NF) composed of poly(vinyl alcohol) (PVA) and <SMALL>D</SMALL>-α-tocopheryl polyethylene glycol succinate (TPGS) was developed for locoregional delivery of phloretin to oral cancers. PVA/TPGS/phloretin NF with 321nm mean diameter and >90% drug entrapment efficiency was fabricated by an electrospinning method. Transformation of drug from crystalline to amorphous state was identified by solid-state studies. NF structure was changed to nanoparticles after its dispersing in the aqueous medium. PVA/TPGS/phloretin NF exhibited fast wetting property and smaller hydrodynamic size of dispersion, compared with PVA/phloretin NF. The amphiphilic property of TPGS also contributed to the improved drug release from PVA/TPGS/phloretin NF. The anticancer activities of phloretin, <I>via</I> the inhibition of glucose uptake into the cancer cells, in NFs were assessed in YD-9 cells (oral squamous cell carcinoma from buccal cheek). The antiproliferation efficacy of PVA/TPGS/phloretin NF was significantly higher than that of phloretin solution and PVA/phloretin NF (<I>p</I> <0.05). Higher apoptotic events were also observed in PVA/TPGS/phloretin NF group rather than phloretin solution and PVA/phloretin NF groups (<I>p</I> <0.05). All these results support that PVA/TPGS/phloretin NF can be a promising fast dissolving formulation for the treatment of oral cancers.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Attenuated Proliferation and Migration of Rat Aortic Smooth Muscle Cells into Epigallocatechin-3-O-gallate-Loaded Collagen Matrices

한동욱,박종철,임혜련,조한희,Kazuaki Matsumura,백현숙,이미희,우연이,현성휴 한국생체재료학회 2006 생체재료학회지 Vol.10 No.1

The migration of vascular smooth muscle cells (VSMCs) from the tunica media to the subendothelial region is a key event in the development and progression of atherosclerosis and post-angioplasty vascular remodeling. The abnormal growth of VSMC also plays an important role in vascular diseases, including arteriosclerosis and restenosis after angioplasty. Many in vitro assays have shown that ()-epigallocatechin-3-O-gallate (EGCG) has antiproliferative effects on various cells. In this study, the proliferation of rat aortic SMCs (RASMCs) with serum stimulation were investigated into EGCG-loaded collagen matrices (EGCG-CM). Also, the effect of EGCG-CM was examined on the migration of serum-stimulated RASMCs. RASMCs were primarily cultured from rat aorta and then characterized by using immunocytochemical analysis with -smooth muscle actin antibody. EGCG treatment to RASMCs showed a dose-dependent decrease in cell viability as increase in its concentration. EGCG-CM was fabricated by freeze-drying at -40oC and then loaded with EGCG. The proliferation of RASMCs cultured into EGCG-CM was significantly (p < 0.05) attenuated in spite of serum induction (reduction by about 30% after 3 d of cultures and 39% after 5 d). While RASMCs migrated toward CM in response to serum and populated it with essentially uniform ingrowth, EGCG-CM considerably blocked the cell migration after 3 d of incubation. These results suggest that reduction in the proliferation and migration of RASMCs induced by serum may be mediated through the inhibitory effects of EGCG released from CM

기생초 꽃 추출물의 생리활성

황인국(In Guk Hwang),김현영(Hyun Young Kim),신소림(So Lim Shin),이철희(Cheol Hee Lee),이준수(Junsoo Lee),장금일(Keum Il Jang),정헌상(Heon Sang Jeong) 한국원예학회 2010 원예과학기술지 Vol.28 No.5

기생초 꽃의 생리활성을 평가하여 천연물 유래 기능성 소재를 개발하기 위하여 에탄올 추출물 및 용매분획물의 항산화활성, ACE 저해활성, α-glucosidase 억제능, 항염활성 및 항암활성을 측정하였다. 에탄올 추출물의 DPPH radical 소거활성(IC??)과 ABTS radical 소거활성은 각각 0.100㎎ · mL?¹ 및 3.427 AEAC였으며, 용매분획물 중 ethyl acetate 분획물이 각각 0.034㎎ · mL?¹ 및 15.785AEAC으로 가장 높았으며, ACE 저해활성 및 α-glucosidase 억제활성도 각각 40.96% 및 0.125㎎ · mL?¹로 ethyl acetate 분획물이 우수하였다. 또한 에탄올 추출물, chloroform 및 ethyl acetate 분획물에서 세포독성 없이 효과적으로 NO의 생성을 억제하였고, 대장암 세포주에 대한 에탄올 추출물, n-hexane, chloroform 및 ethyl acetate 분획물의 IC??값은 각각 0.208, 0.041, 0.142 및 0.107㎎ · mL?¹으로 우수한 증식억제효과를 보였다. 이러한 결과를 종합하여 볼 때, 기생초 꽃의 우수한 생리활성을 이용한 기능성 소재로의 활용이 가능 할 것으로 판단된다. This study was conducted to evaluate the antioxidant activity, Angiotensin I Converting Enzyme (ACE) inhibitory activity, α-glucosidase inhibitory activity, nitrate synthesis inhibitory activity, and antiproliferation inhibitory effect on ethanol extract and its solvent fractions of Coreopsis tinctoria Nutt. Ethyl acetate fraction was the strongest at 1,1-diphenyl-2-picryl hydrazyl (DPPH) (IC?? = 0.100 ㎎ · mL?¹) and 2,2‘-Azino-bis-(3-ethylbenozothiazoline-6-sulfonic acid) (ABTS) (15.785 ㎎ AA eq · 10 ㎎?¹) radical scavenging activity, ACE (40.96% at 1 ㎎ · mL?¹), and α-glucosidase (IC?? = 0.125 ㎎ · mL?¹) inhibitory effect among the solvent fractions. Nitrate synthesis inhibitory activity of ethanol extract, chloroform fraction, and ethyl acetate fraction effectively inhibited NO formation in a dose-dependent manner without the cytotoxic effect. Ethanol extract and its solvent fractions inhibited growth of HCT-116 colon cancer cells in a dose-dependent manner. n-Hexane fraction showed the highest antiproliferation inhibitory effect of 0.041 ㎎ · mL?¹ among fractions.

소목(Caesalpinia sappan L.)에서 분리한 Brazilin의 항산화 활성과 구강상피암 KB 세포주의 증식억제효과

전미애 ( Mi Ae Jeon ),권현정 ( Hyun Jung Kwon ),김용현 ( Yong Hyun Kim ),한국일 ( Kook Il Han ),정하나 ( Ha Na Chung ),정성균 ( Sung Kyun Chung ),방인석 ( In Seok Bang ),한만덕 ( Man Deuk Han ) 한국치위생과학회 2010 치위생과학회지 Vol.10 No.5

In Vitro Antiproliferative and Apoptosis Inducing Activity of Bibimbap on HT-29 Human Colorectal Adenocarcinoma Cells

Tae Eun Guon,정하숙 한국식품영양학회 2022 韓國食品營養學會誌 Vol.35 No.6

The present study was designed to investigate the antiproliferative activity and molecular mechanisms of Bibimbap in HT-29 human colorectal adenocarcinoma cells. Bibimbap extract inhibited the proliferation of HT-29 cells by 50% at a concentration of 10.1±0.17 mg/mL for 48 h. The population of live cells decreased slightly, and the morphology changed with a reduction in cell volume (pyknosis) with Bibimbap. Treatment with 5 mg/mL of Bibimbap resulted in slight cell shrinkage. Furthermore, as the Bibimbap dose increased to 10 mg/mL, these characteristics were more evident, and HT-29 cells exhibited partial detachment by staining with the DNA-binding dye Hoechst 33342. Flow cytometric analysis by Annexin V and PI double staining showed that Bibimbap increased the levels of apoptosis. Analysis of the mechanism of these events showed that Bibimbap-treated cells exhibited a mitochondria-dependent apoptotic pathway through the modulation of caspase-3, caspase-8, caspase-9, and poly-ADP ribose polymerase, as well as Bax and Bcl-2 expression in dose- and time-dependent manners. Consequently, Bibimbap exerts a significant antiproliferative effect on HT-29 human colorectal adenocarcinoma cells. Key words: Bibimbap, antiproliferation, HT-29, human colorectal adenocarcinoma cells, mitochondrial apoptosis, Korean dish