Black stain을 가진 유치 치태에서 추출한 방선균의 S. mutans에 대한 항생능 평가

박수진,김신,정태성,김재문 大韓小兒齒科學會 2009 大韓小兒齒科學會誌 Vol.36 No.1

본 연구는 black stain을 가진 유치 치태에서 추출한 방선균이 S. mutans에 대해 항생능을 가지는가를 평가하고자 시도되었다. 만 세 유치열기 어린이 중 black stain이 모든 치아에 존재하는 4명의 어린이를 대상으로 치태를 채취한 후, 증균 배양하여 16개의 방선균 균주를 분리해 냈으며, 이들이 생산해내는 물질이 항생능을 가지는지를 paper disc method를 이용하여 확인하여, 다음과 같은 결론을 얻었다. 1. No.1 균주와 No.5 균주에서 S. mutans에 항생능을 보였으며, 특히 No.5 균주는 Oxacillin과 유사한 항생능을 보였다. 2. No.1 균주와 No.5 균주는 일반적으로 항생능 평가시 사용되는 시험균인 Bacillus cereus, Bacillus subtilis에도 항생능을 보였다. 3. PCR을 통해 구강내 대표적인 상주 방선균들을 대상으로 이 균주들의 동정을 시도해본 결과 No.5 균주는 Actinomyces viscosus와 97% 일치하는 것으로 나타났다. The aim of this study is to assess the antibiotic activity of Actinomyces in plaque from black stained primary teeth to Streptococcus mutans. Samples were obtained from four children, 2-6 years of age, who had black stains on all erupted primary teeth. 16 different Actinomyces spp. were isolated, and antibiotic activity test with paper disc method was done. The results were as follows, 1. No.1 and No.5 Actinomyces spp. showed the antibiotic activity to Streptococcus mutans and the activity of No.5 Actinomyces spp. could compete with that of Oxacillin. 2. No.1 and No.5 Actinomyces spp. also exhibited the antibiotic activity to Bacillus cereus, Bacillus subtilis commonly used as experimental bacteria for testing antibiotic activity. 3. For identification of No.1 and No.5 spp., PCR analysis was done. No.5 spp. matched Actinomyces viscosus at 97% level but No.1 spp. didn’t match.

저염분 노출에 따른 감성돔( Acanthopagrus schlegelii ) 아가미의 Na+/K+-ATPase 활성 및 발현

민병화,박미선,명정인,서정수,박정준,노경언,강덕영 한국수산과학회 2015 한국수산과학회지 Vol.48 No.1

We investigated the branchial osmoregulatory response of black sea bream Acanthopagrus schlegelii to short-term (3-48 h) exposure to a hyposaline environment (5 psu). Gill Na+/K+-ATPase (NKA) activity was decreased after 3 h in fish transferred to 5 psu compared to salt water-acclimated (control) fish, but the level of activity returned to that observed in the control fish at 6 h after transfer. NKA activity increased significantly at 24 h after transfer, but it returned to the level observed in the control fish at 48 h after transfer. Immunohistochemical staining revealed that gill NKA was localized to chloride cells. The number of chloride cells tended to change in parallel with NKA activity. Substantial decreases in plasma Na+, Cl-, and osmolality were observed after 12 h of exposure to 5 psu; however, these parameters began to recover to the values detected in the controls at 24 h after transfer. In conclusion, our results suggest that black sea bream are able to adjust their osmoregulatory mechanisms to shift from hypo- to hyperosmoregulation within 6 h of exposure to a hypoosmotic environment.

요추부 퇴행성 추간판의 자기공명 영상소견에 따른 체외배양 수핵세포주의 노화 인자와의 관련성에 대한 연구

윤상훈 ( Sang Hoon Yoon ),임정희 ( Jeong Hee Im ),김기정 ( Ki-jeong Kim ),조병규 ( Byung-kyu Cho ) 국군의무사령부 2018 대한군진의학학술지 Vol.49 No.1

Objectives: The authors conducted this study to investigate the relationship between signal to noise ratio in T2 weighted signal intensity of intervertebral disc (IVD) on MRI and biomarkers associated with senescence changes. Methods: Five patients (5 male) who underwent open discectomy or posterior lumbar discectomy and fusion for symptomatic herniated nucleus pulposus (NP) were included. The NP specimens were grouped according to a grading system for IVD degeneration based on the preoperative MRI and SNR was measured. The SA-b-gal activity of the NP cells was determined and the percentage of SA-b-gal-positive NP cells were calculated. The telomerase activity was analyzed and the amount of TRAP products was determined by measurement of their absorbance at 450 nm. Results: The mean patient age was 35.2 years (range: 21-52 years, SD ±17.283). There was no significant correlation between every age and SNR (Spearman’s test, r2=0.440, p=0.111). As degenerative changes became more distinct, decrement of SNR showed each senescence marker changed to the same tendency in the patient with age of fifties. The correlation between SNR and SA-b-gal was not statistically significant. However, SNR was slightly correlated with SA-b-gal. (R2 = 0.0404, p = 0.373) Despite the lack of statistical significance, there was a negative correlation between telomerase activity and SNR. (R2 = 0.3103, p = 0.165) In aged group of twenties, the means of the percentages of the SA-b-gal-positive NPCs from the patients aged 21, 21, and 26 years were 63.25%, 42.21%, and 62.27%, respectively, at passage 3 and the means of the percentages of the SA-b-gal-positive NPCs from the patients aged with fifties were 56.97% and 71.84% respectively at passage 3. There was a steady decline in the telomerase activity of the NP cells with advancing passages, irrespective of patient age. However, this tendency did not have statistical significance. (p>0.05) Conclusions: The NP cells in aging discs exhibited characteristic senescent features such as an increased SA-b-gal expression and decreased telomerase activity. SNR is associated with degenerative changes of NP, and as it increases, the number of SA-b-gal positive cells increases and telomerase activity tends to decrease.

Characteristics of Pseudomonas brenneri Isolated from Korean Raspberry

Kyong-Min SEOK,Jeong-Ah YOON,Soo-Hwan YEO,Myoung-Dong KIM 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10

To find unrecorded domestic species, wild-type strains were isolated from Raspberry collected in Daeryong Mountain at Chuncheon. Among the 1,015 isolates, NIB 325 showed antimicrobial activity against Bacillus cereus and identified as Pseudomonas brenneri, which had not been domestically reported. The biochemical characteristics of the strains were examined by using an API 20NE and API ZYM KIT. API ZYM KIT results suggested that NIB 325 strain is active in esterase(C4), unlike the previously reported JNP 2044 strain. Esterase is a valuable enzyme used in amino acid synthesis, steroid conversion, and ester synthesis processes. Esterase activity of P. brenneri NIB 325 was qualitatively observed by the activity-staining method. Further study might be followed by characterization of the enzyme activity and optimization of cultivation conditions.

Molecular Cloning and Characterization of Catechol 2, 3-Dioxygenase Gene from Aniline-Degrading Psseudomonas acidovorans

Lee, Ji-Hyun,Bang, Sung-Ho,Park, Youn-Keun,Lee, Yung-Nok The Microbiological Society of Korea 1992 미생물학회지 Vol.30 No.4

Catechol 2, 3-dioxygenase (C230) catalyses the oxidative ring cleavage of catechol to 2-hydroxymuconic semialdehyde. This is one of the key reactions in the metabolism of the widespresd pollutant aniline. We have cloned a gene encoding C230 from cells of the aniline degrading bacteria, Pseudomonas acidovorance KCTC2494 strain and expressed in E. coli, A 11.3-kilobase Sau3A partial digested DNA fragment from KCTC2494 was cloned into phagemid vector pBluescript and designated as pLP201. The C230 gene was mapped to a 2.8-kb region, and the derection of transcription was determined. The cloned C230 gene contains its own promoter which can be recognized and employed by E. coli transcriptional apparatus. C230 activities of subclones were identified by enzyme assay and activity staining. The T7 RNA promoter/polymerase system and maxicell analysis showed that a polypeptide with Mw of 35 kDa is the C230 gene product.

木材 變色菌 및 表面汚染菌類에 對한 Streptomyces rimosus 代謝物質의 抗菌 效力

姜奎榮,金思翼,吳正壽 동국대학교 산업기술환경대학원 1994 산업기술논총 Vol.2 No.-

The objectives of this study were to evaluate the efficacy of metabolites produced by Streptomyces rimosus in protecting wood against wood staining and mold fungi. The following representative wood staining and mold fungi were selected for evaluate the antifungal activities of metabolites from Streptomyces rimosus : staining fungi - Ceratocystis pilifera, Ceratocystis piceae, Aureobasidium pullulans ; mold fungi - Trichoderma hazianum, Trichoderma viride, Penicillium citrinum, Aspergillus niger. The effect of medium concentration on development of antifungal metabolites from Streptomyces rimosus was examined in plate bioassay against spores of test fungi. The antifungal activity of these metabolites tested using plate bioassay. These results are summarized as follows ; 1. In the plate bioassay, the conidial germination of wood staining fungi was completely inhibited, leaving a clear zone around the paper disc treated with metabolites from Streptomyces rimosus. 2. The most effective antifungal activity showed in Aureobasidium pullulans. 3. The best result of different concentration of metabolites production medium was obtained with the ⅓×concentration in the largest clear-zone area around the paper disc. The next largest clear-zone area occurred with the ½×concentration.

Activity Staining of Glutathione Peroxidase after Two-dimensional Gel Electrophoresis

고창원,박병철,박성구,이도희,조사연,오구택,강성만 한국분자세포생물학회 2004 Molecules and cells Vol.18 No.3

We have developed a method for rapid activity staining of proteins with glutathione peroxidase (GPx) activity after 2-D gel electrophoresis. After separating proteins extracted from yeast, or mouse red blood cells, by two-dimensional gel electrophoresis, SDS was removed and the gel was submerged in a Tris-HCl buffer containing glutathione and hydrogen peroxide, followed by incubation with 3-(4,5-dimethylthiazol-2-yl)- 2,5-diphenyltetrazolium bromide (MTT) and phenazine methosulfate (PMS). After this proteins with GPx activity appeared as clear zones on a purple background. This relatively simple activity staining method could be useful for rapid screening of proteins with GPx activity in cell extracts.

Xanthomonas sp. YL-37의 Alkaline Protease 유전자의 클로닝

이대희,김수경,이승철,윤병대,황용일 한국산업미생물학회 1995 한국미생물·생명공학회지 Vol.23 No.2

생분해성의 세제를 개발하기 위해 alkaline protease 생산성 균주인 Xanthomonas sp. YL-37에서 분리한 염색체 DNA와 plasmid pUC9를 이용하여 대장균에서의 gene bank를 작성하여, 배지상에서 alkaline protease 생산성 균주를 분리하였다. Agarose gel 전기영동과 제한효소지도로부터 약 2.7 kb의 alkaline protease 구조유전자가 포함된 DNA 단편이 pUC9 내에 클로닝된 것을 확인하였다. 배양상등액을 이용한 SDS-PAGE 상에서의 activity staining assay 결과로 alkaline protease가 세포외로 분비됨을 확인하였으며 분자량은 약 64 kDa으로 추정되었다. 생산된 alkaline protease 활성은 20℃, 30℃, 40℃ 에서 차이를 보이지 않았다. 이들 결과로부터 클로닝된 plasmid를 함유하는 대장균에서 생산된 alkaline protease는 Xanthomonas sp. YL-37의 alkaline protease와 같음을 알 수 있었다. For the purpose of developing a new biodegradable detergent, we have isolated a gene encoding wide-range temperature applicable alkaline protease from Xanthomonas sp. YL-37 (Lee et al., 1994, Kor. J. Appl. Microbio. Biotechnol.) An alkaline protease gene was isolated from the gene bank that was prepared from the chromosomal DNA of Xanthomonas sp. YL-37. From the results of agarose gel electrophoresis and a restriction enzyme mapping, a 2.7 kb DNA fragment containing the alkaline protease gene was inserted in the plasmid pUC9. Extracellular activity of a clone having alkaline protease gene was detected on SDS-polyacrylamide gel with activity staining assay. The molecular weight of alkaline protease was determined to be about 64 kDa from 11% SDS-PAGE analysis. Alkaline protease activity, produced from E. coli which harboring the plasmid, showed no difference at reaction temperature 20, 30 and 40℃, respectively. This result showed that alkaline protease produced from E. coli harboring the plasmid was apparently the same as that of Xanthomonas sp.YL-37.

Thermal Inactivation of Myrosinase from White Mustard Seeds

Ko, Young Hwan,Lee, Ran The Korean Society of Food and Nutrition 2021 韓國食品營養學會誌 Vol.34 No.1

Myrosinases (thioglucosidases) catalyze the hydrolysis of a class of compounds called glucosinolates, of which the aglycones show various biological functions. It is often necessary to minimize the loss of myrosinase activity during thermal processing of cruciferous vegetables. Myrosinase was isolated from a popular spice, white mustard (Sinapis alba), and its thermal inactivation kinetics was investigated. The enzyme was extracted from white mustard seeds and purified by a sequential processes of ammonium sulfate fractionation, Concanavalin A-Sepharose column chromatography, and gel permeation chromatography. At least three isozymes were revealed by Concanavalin A-Sepharose column chromatography. The purity of the major myrosinase was examined by native polyacrylamide gel electrophoresis and on-gel activity staining with methyl red. The molecular weight of the major enzyme was estimated to be 171 kDa. When the consecutive step model was used for the thermal inactivation of the major myrosinase, its inactivation energy was 44.388 kJ/mol for the early stage of destruction and 32.019 kJ/mol for the late stage of destruction. When the distinct two enzymes model was used, the inactivation energy was 77.772 kJ/mol for the labile enzyme and 95.145 kJ/mol for the stable enzyme. The thermal inactivation energies lie within energy range causing nutrient destruction on heating.

Characterization of Endochitosanases-Producing Bacillus cereus P16

( Yu Young Jo ),( Kyu Jong Jo ),( Yu Lan Jin ),( Woo Jin Jung ),( Ju Hee Kuk ),( Kil Yong Kim ),( Tae Hwan Kim ),( Ro Dong Park ) 한국미생물생명공학회 2003 Journal of microbiology and biotechnology Vol.13 No.6