Colonization of Listeria monocytogenes in potting soils as affected by bacterial community composition, storage temperature, and natural amendment

윤재현,김솔아,심원보,서동철,최송이,이선영,김세리 한국식품과학회 2021 Food Science and Biotechnology Vol.30 No.6

This study aimed to characterize the bacterialcommunity of commercial potting soils with or withoutListeria monocytogenes inoculation at 5–35 C using 16Smetagenomic sequencing and evaluate the effect of naturalamendments on the reduction L. monocytogenes in nonsterilepotting soils. An increase in the expected operationaltaxonomic units of each sample with or without L. monocytogenes was proportional to the increasing storagetemperatures after 5 days. Biodiversity was distinct amongall potting soils for Shannon and inverse Simpson indices,with the highest diversity being observed in a soil samplestored at 35 C for 5 days with L. monocytogenes. Anincrease in richness and diversity of soil bacterial communitystructure positively correlated with less survival ofthe invading L. monocytogenes. Particularly, garlic extractwas demonstrated as a promising soil-amendment substrate,reducing L. monocytogenes by C 4.50 log CFU/g inpotting soils stored at 35 C.

NaCl 및 N.P.K處理別 가는갯능쟁이 無機養分 吸收에 關한 硏究

金寬洙,文溶植 대전대학교 기초과학연구소 1991 自然科學 Vol.2 No.-

NaCl및 N.P.K.處理에 依한 土壤의 無機成分含量變化와 가는갯능쟁이의 養分吸收및 生長에 미치는 影響을 究明하고자 實驗을 통해 얻어진 結果를 要約하면 다음과 같다. NaCl處理量이 增加함에 따라 Pot土壤 硬固度가 높았고 pH가 약간 增加하였으며 pH가 上昇함에 따라 土壤中 T-N, P₂O?, SO₄, Zn 및 Mn含量이 減少된반면 Ca, Cu 및 SiO₂含量은 增加되었다. Pot土壤中 NaCl含量은 植物體中 T-N, Na₂O, Cl, SO₄ 및 Fe吸收量을 增加시킨 반면 P₂O??, K₂O, CaO, Zn 및 SiO₂吸收量은 減少시켰다. 植物體中 Na₂O吸收量이 增加함에 따라 T-N, Cl, Fe 및 Mn吸收는 增加된 반면 P₂O??, K₂O, CaO, MgO 및 Zn 吸收는 低下되었으며 植物體中 Cl吸收量 역시 Na₂O에서와 같은 傾向을 나타냈다. 耐鹽性 程度는 無肥料區에 比해 N.P.K. 處理區에서 增大되었다. 3要素 平均利用率은 無鹽分區에 比해 1.0% NzCl處理區에서 增加되어 N19.7%, P36.0% , K62.2%였다. This study was to explain the influences which affect changes in the content of mineral nutrition, nutrition up take and growth of Atriplex gmelimii in soil with NaCl and N.P.K. treatment. As the amount of NaCl treatment increased, pot soil hardness increased and the pH was increased slightly. As pH increased, the content of T-N, P₂O?, SO₄, Zn and Mn decreased but that of Ca, Cu and SiO₂ increased in the soil. The content of NaCl in pot soil increased the absorption of T-N, Na₂O, Cl, SO₄, and Fe in the plantbody but decreased the amount of absorption of P₂O?, K₂O, CaO, Zn and SiO₂. In the plantbody, as the amount of absorption of Na₂O increased, the absorption of T-N, Cl, Fe and Mn decreased. The amount of absorption of Cl showed the same tendency as Na₂O. The degree of salt tolerance increased in the N.P.K. treatment plot more than in the unfertilizer plot. The average utilization rate of N.P.K. increased more in the 1.9% NaCl treatment plot than in the untreated plot. N was 19.7%, P 3.6% and K 62.2%.

Treatment of Acidic Mine Soils: Effects on Heavy Metal Dynamics and Growth of Corymbia Citriodora Var.variegata Seedlings

Yong Liu,Yingqun Ma,Chuxia Lin 한국토양비료학회 2014 한국토양비료학회 학술발표회 초록집 Vol.2014 No.6

Effects of sterilization methods on the survival of pathogenic bacteria in potting soil stored at various temperatures

현정은,이수빈,정도영,김세리,최송이,황인준 한국식품과학회 2023 Food Science and Biotechnology Vol.32 No.1

Fresh food products can be contaminated with pathogenic bacteria in various agricultural environments. Potting soil is sterilized by heat sterilization and then reused. This study evaluated the effects of three sterilization methods (non-sterilized, pasteurized, and sterilized) on the survival of pathogenic bacteria in potting soil during storage for 60 days at 5, 15, 25, and 35 °C. The reduction in Escherichia coli O157:H7, Salmonella Typhimurium, and Staphylococcus aureus in potting soil was higher at higher temperatures (25 and 35 °C) than at lower temperatures (5 and 15 °C). The population of pathogenic bacteria in pasteurized and sterilized potting soil was reduced below the detectable levels within 30 days at 35 °C. In contrast, the population of Bacillus cereus did not change in potting soil during storage for 60 days at all temperatures. These results indicate that sterilization and storage temperature of potting soil are critical factors influencing the survival of pathogenic bacteria.

도시농업용 배양토의 조성과 용기의 형태가 몇 가지 초본 및 목본류의 묘 생육에 미치는 영향

권영휴,김수진,오하경,한상균,김석진 한국인간·식물·환경학회 2016 인간식물환경학회지 Vol.19 No.5

This study was conducted to examine effects of media kinds and container forms for urban agriculture on the growth of herbaceous and woody plants that could be used in construction of urban greenery and landscape gardens. The main objective of the study was to investigate the most efficient culture soil and container form for four herbaceous and one woody plant species. In this study, five different species were examined on three different media kinds [horticultural substrate (HS), decomposed granite (DG), and horticultural substrate + decomposed granite (HS + DG)] with two different container forms [vinyl pot (Ø7.5 cm) and plug tray (20 cells)]. The results indicated that pH values of media of HS and HS + DG were 5.8 and 5.9, respectively while DG was 6.5. HS had the highest electrical conductivity with 0.90 dS・m -1 while HS + DG and DG had 0.39 and 0.08 dS・m -1 , respectively. Especially, most of the species experimented on HS tended to have the highest growth rate in the plant height, the root length, the number of leaves and fresh biomass weight compared to other soil kinds (P<0.05). The growth rate on HS + DG was much higher than DG. Therefore, our results support that HS + DG could be possible to replace with HS. In addition, there had no differences on growth rates between container forms (P>0.05). The use of plug tray could be more applicable than the use of vinyl pot containers, considering growth rates of plants and convenient of shipment on plant production.

가는갯능쟁이 無機養分吸收에 미치는 NaCl 및 肥料의 效科

文溶植,金寬洙 대전대학교 자연과학연구소 1992 自然科學 Vol.3 No.-

가는갯능쟁이에 대한 無機養分吸收 樣相을 밝히고 NaCl과 肥料處理에 依한 土壤의 無機成分 含量變化와 植物의 養分吸收 및 生長에 미치는 影響을 究明하고자 實驗을 實施한 結果를 要約하면 다음과 같다.NaCl을 施用함에따라 T/R比는 減少되었으며 土壤中 Cl含量 增加는 뿌리의 伸長을 增大시켜 根長對 草長比가 增加되었고 Na에서도 Cl가 같은 傾向을 나타냈다. 土壤中 NaCl含量은 植物體中 T-N,Na₂O, Cl, SO₄ 및 Fe 吸收量을 增加시킨 반면 P₂O?, K₂O, CaO, Zn 및 SiO₂吸收量은 減少시켰다. 植物體中 Na₂O 吸收量이 增加함에 따라 T-N, Cl, Fe 및 Zn 吸收는 低下되었으며 植物體中 Cl 吸收量 역시 Na₂O에서와 같은 傾向을 나타냈다. 鹽性程度는 肥料種類에 따라서 相異하였으며 肥料種類間 N,K理容率은 Cl보다 S를 含有한 肥料區에서 낮았다. 生長은 黃酸암모늄區보다 尿素處理區보다 鹽化加里區에서 良好하였으며 石膏處理區에서 가장 良好하였다. This study was carried out on the mineral nutrition absorption of Atriplex gmelinii and to explain the influences which affect changes in the content of miner nutrition, nutrition up take and growth of plants in soil with NaCl and fertilizer treatment. As the NaCl concentration increased the T/R ratio increased. An increased of Cl increased growth in root in pot soil, so the ratio of root length and plant height increased. The same tendency appeared in Na as in Cl. The content of NaCl in pot soil increased the absorption of T-N, Na₂O, Cl, SO₄ and Fe in the plantbody but decreased the amount of absorption of P₂O?, K₂O, CaO, Zn and SiO₂. In the plantbidy, as the amount of absorption of Na₂O increased, the absorption of T-N, Cl, Fe, Mn decreased. The amount of absorption of Cl showed the same tendency as Na₂O. The degree of salt tolerance differed according to the kinds of fertilizer. The utilization rate of N and K dependent on kinds of fertilizer was lower in the fertilized plot which included more S than Cl. The growth of Atriplex gmelinii was better in the urea treatment plot than in the ammonium suphate treatment plot and better in the potassium chloride treatment plot than in the potassium sulfate treatment plot and much better in the gypsum treatment plot.

서향의 삽목번식 방법과 실내도입을 위한 광, 토양에 관한 연구

노나영(Na Young Ro),고호철(Ho Chul Ko),허온숙(On-Sook Hur),강만정(Man-Jung Kang),오세종(Se-Jong Oh),허윤찬(Yun-Chan Huh) (사)한국생물환경조절학회 2011 생물환경조절학회지 Vol.20 No.4

본 실험은 자생식물인 서향의 실내 분화로의 도입을 위해 대량 번식 및 광, 토양 조건 구명 실험을 수행하였다. 서향의 대량 번식을 위해 적합한 삽목 상토, 발근촉진제의 농도, 삽목 시기를 찾는 실험을 하였고, 실내로 도입하기 위해 인공광 처리(100, 1000, 2500lux)와 분화상토처리(원예용 혼합상토(바로커), 펄라이트 + 원예상토(1 : 1), 송이 + 원예상토(1 : 1), 마사토 + 원예상토(1 : 1), 모래 + 원예상토(1 : 1))를 하였다. 삽목 상토 선발하기 위해 펄라이트, 버미큘라이트, 펄라이트와 버미큘라이트 혼용(1 : 1), 원예용상토 처리를 한 결과에서는 펄라이트와 버미큘라이트 혼용(1 : 1)처리 구에서 발근율이 93.3% 가장 우수하였다. 발근촉진제를 농도별로는 루톤과 IBA 100ppm 처리에서 뿌리 생육이 가장 양호하였다. 녹지삽 시기는 7월에 하는 것이 발근율이 96.7%로 우수하였다. 화분 이식 후 1000lux 이상의 광 조건에서 생육이 가장 좋았으며, 분화용 상토로는 제주 송이와 원예용 상토를 혼합한 토양에서 생육이 가장 좋았다. This research was done to identify the mass propagation method in winter daphne (Daphne odora Thunb) using its softwood cuttings and to investigate its plant characters established at different light and soil conditions as an indoor plant. Cuttings from winter daphne were taken and grown in different treatment consisted of rooting media (perlite, vermiculite, perlite + vermiculite (1 : 1) and commercial horticulture media soil), indole butyric acid (IBA) hormone concentrations (0, 100, 500, 1000 ppm and Rootone) and date of cutting. Transplants were grown at different light intensities (100, 1000, 2500 lux and control) and growing media. Results showed that cuttings grown in perlite + vermiculite (1 : 1) gave higher percentage (100%) rooting. Cuttings treated with Rootone and IBA 100 ppm showed good rooting growth and cutting taken in June, 25 gave the highest rooting (96.7%). The best plant growth obtained at 1000 lux (56~60 μ㏖ㆍm?²ㆍs?¹) followed by 2500 lux (125~130 μ㏖ㆍm?²ㆍs?¹) and scoria mixed with commercial horticulture media soil showed better growth of transplants.

Uniconazole의 처리농도 및 방법이 참나리의 생장과 개화에 미치는 영향

정정학,권순태 한국화훼산업육성협회 2005 화훼연구 Vol.13 No.1

생물반응기를 통해 대량증식된 사계성 딸기 ‘고하’ 소식물체의 순화용기에 따른 생육특성과 묘생산 경제성

이종남,김혜진,김기덕,임주성,임학태,용영록 한국원예학회 2012 원예과학기술지 Vol.30 No.4

본 실험은 생물반응기를 통해 대량증식된 사계성 딸기 조직배양묘의 적정 순화용기를 선발하기 위해 실시하였다. 순화용기는 플라스틱투명용기, 플러그 트레이, I-포트 및 흑색비닐포트 등 4가지를 사용하였다. 토양함수율 10%이상 지속일수는 플라스틱 투명용기가 5일, 흑색비닐포트가 3-4일, 플러그 트레이 2일, I-포트가 1일 순이었다. 생존율은 흑색비닐포트가 80%, 플라스틱투명용기가 70%, I-포트가 55% 및 플러그 트레이가 15% 순으로 나타났다. 또한 생육량은 플라스틱 투명용기가 가장 왕성하였고, 흑색비닐포트와 플러그 트레이는 비슷하였으며, I-포트가 가장 저조하였다. 런너 발생은 흑색비닐포트가 주당 3.3개로 가장 많았으며, 플라스틱투명용기가 2.9개, 플러그 트레이가 1.6개 및 I-포트가 1.2개 순으로 나타났다. 총 생산비용은 흑색비닐포트가 44,405,300원/10a로 I-포트의 51,064,700원/10a보다 6,659,400원/10a이 더 절감되었으며, 총 생산주수도 흑색비닐포트가 102,718주/10a로 I-포트의 72,452.9주/10a보다 30,265.1주/10a 더 생산되었다. 또한 주당 생산비용은 흑색비닐포트가 432.3원으로 I-포트의 704.8원에 비해 272.5원의 감소하였다. 따라서 생물반응기를 통해 대량증식된 사계성 딸기 조직배양묘의 순화용기는 흑색비닐포트가 적당하였다. This study was conducted to find out the optimum container for increasing acclimatization rate of in vitro mass propagated plantlets of Ever-bearing strawberry (Fragaria × ananassa Duch.) via bioreactor. Four types of containers were used such as transparent plastic container (TPC), plug tray (PT), I-pot (IP), and black vinyl pot (BVP). Number of date maintaining soil water content above 10% was five days in TPC, three to four days in BVP, two days in PT, and one day in IP. Survival rate of plantlets was 80% in BVP, 70% in TPC, 55% in IP, and 15% in PT. In TPC, growth increment of plantlets was the greatest among all the tested containers and the lowest in IP. Numbers of runner per plant were 3.3 in BVP, 2.9 in TPC, 1.6 in PT, and 1.2 in IP. Total cost was 44,405,300 won/10 a in BVP, resulting in reducing more 6,659,400 won/10 a than IP’s (51,064,700 won/10 a). Around 102,718 plants/10 a were produced by using BVP, suggesting that 30,265.1 plants/10 a more could be produced than IP (72,452.9 plants/10 a). Production cost per plant was 432.3 won in BVP, resulting in reducing 272.5 won than IP’s (704.8 won). As a result, BVP was appropriate for acclimatization of in vitro plantlets through bioreactor system.

The Effect of Livestock and Food Waste Compost on Rice Yield and Nutrient Utilization Efficiency according to Different Nitrogen Fertilizer Treatments Rates

Song Rae Cho,Ye-Jin Lee,Chan-Wook Lee,Byeong-Geun Hyun,Yo-Sung Song,Chul-Hyun Ryu,Yang Min Kim,Seul-Bi Lee 한국토양비료학회 2021 한국토양비료학회지 Vol.54 No.4

The amount of livestock manure generated was 1,532 hundred tons/day as of 2019, and it increased by about 11% over 10 years. It has been raised as a cause of soil and water pollution due to the continuous overspray of livestock compost on farmland. It is essential to calculate an appropriate amount of livestock compost that can replace chemical fertilizers in order to manage local environment-friendly nutrients without problems caused by the use of livestock compost. In addition, food waste is mostly agricultural products-derived materials, and it is necessary to establish a comprehensive management system for agricultural cycle use. In this study, to confirm the compost mixed with livestock and food waste as an alternative to chemical fertilizer, comparison of the basic replacement of chemical fertilizer and livestock, the replacement of the mixed compost including food waste, the replacement of the total nitrogen amount of livestock compost, and the replacement of the total nitrogen amount of the mixed compost. Experiments were conducted to confirm the effect of each compost on crop production. Commercially available livestock compost (_L) and mixed compost (food waste and live-stock)(_M) were used as the material for the experiment. Treatments were control, NPK, 5N_L, 5N_M, 7.5N_L, 7.5N_M, 9N_L, 9N_M. The comparison between livestock compost and mixed compost, mixed compost has 30% high crop productivity. The nitrogen use efficiency was shown as 7.5N_M > 5N_M > 5N_L > NPK > 7.5N_L > 9N_M > 9N_L. For high yield and nutrient uptake, fertilizer partial replacement (55.6% or 83.3% than total replacement) will be more efficient than 100% total nitrogen requirement replacement.