임신주령과 진통에 따른 임부 혈청 C-Reactive Protein의 동태

이승호,이태형,김병석,이영기,이재열,김종호 영남대학교의과대학 1993 Yeungnam University Journal of Medicine Vol.10 No.2

임신주령과 진통 및 분만의 진행정도에 따른 정상 임부의 혈청 CRP치의 동태를 파악하기 위해서 1992년 3월 1일 부터 1993년 8월 31일 까지 18개월간 영남대학교 의과대학 부속병원 산부인과에서 임신 20주에서 44주 사이의 건강한 임부 521명을 대상으로 혈청 CRP치를 측정하여 임신주령과 진통의 유무, 양막의 파열여부 및 분만의 진행정도에 따라 비교 분석한 결과는 다음과 같다. 1. 혈청 CRP치 0.8㎎/㎗ 이상과 2.0㎎/㎗이상을 나타낸 임부의 빈도는 각각12%(61/521)와 4%(22/521)이었다. 2. 임신주령 37주이하의 만삭전 임부군과 38주이상의 만삭임부군, 양막 파열 임부군과 비파열 임부군, 자궁경관 개대 3㎝이하 임부군과 4㎝이상 임부군의 비교에서 혈청 CRP치 0.8㎎/㎗ 이상과 2.0㎎/㎗ 이상을 나타낸 임부의 빈도는 통계적으로 유의한 차이가 없었다. 3. 진통이 없는 임부군과 진통중인 임부군의 비교에서 혈청 CRP치 0.8㎎/㎗ 이상을 나타낸 임부의 빈도는 각각 5.93%와 13.73%로 통계적으로 유의한 차이(p<0.05)가 있었으나 혈청 CRP치 2.0㎎/㎗ 이상을 나타낸 임부의 빈도는 차이가 없었다. 4. 임신주령 37주 이하의 만삭전 임부군에서 혈청 CRP치 0.8㎎/㎗ 이상을 나타낸 임부의 빈도는 진통중인 임부군이 23.64%로 진통이 없는 임부군의 4.69%보다 현저히 높았으며(p<0.001), 혈청 CRP치 2.0㎎/㎗ 이상을 나타낸 임부의 빈도도 진통중인 임부군과 진통이 없는 임부군이 각각 12.73%와 3.13%로 통계적으로 유의성 있는 차이(p<0.05)가 있었다. 임신주령 38주 이상의 만삭 임부군에서 진통이 없는 임부군과 진통중인 임부군을 비교하여 혈청 CRP치 0.8㎎/㎗ 이상과 2.0㎎/㎗ 이상을 나타낸 임부의 빈도는 통계적으로 유의성 있는 차이가 없었다. 이상 본 연구의 결과를 종합하면 임부의 감염을 조기 예측하는데 있어 기왕에 조기진통 임부에서 임상적 감염의 지표로 이용되고 있는 혈청 CRP치 2.0㎎/㎗은 진통중인 만삭임부에서도 양막파열여부와 분만의 진행정도와 무관하게 유용한 것으로 추정되며, 조기진통 임부의 처치시 혈청 CRP치가 0.8㎎/bl 이상인 경우 임부의 불현성 감염을 의심하여야 할 것으로 생각된다. In order to evaluate the clinical usefulness of maternal serum C-reactive protein measurement in early detection of infectious morbidity at term laboring women, serum C-reactive protein levels were measured in 521 healthy pregnant women; 64 who were not in labor before term, 55 who were in labor before term, 71 who were not in labor at term and 331 who were in labor at term. The frequencies of elevated serum C-reactive protein level were compared in relation to the gestational weeks, the presence or absence of labor, the status of amniotic membranes and the degree of cervical dilation. The obtained results were as follows. 1. The frequencies of women with elevated serum C-reactive protein, 0.8㎎/㎗ or higher and 2.0㎎/㎗ or higher, in 521 health pregnant women were 12% and 4%, respectively. 2. C-reactive protein levels of 0.8㎎/㎗ or higher were more frequent in the group of women in labor than those not in labor(5.93%, vs. 13.73%, p<0.05), but the frequencies of C-reactive protein level of 2.0㎎/㎗ or higher were not statistically different between both groups. The frequencies of C-reactive protein level of 0.8㎎/㎗ or higher and 2.0㎎/㎗ or higher were not statistically different between the groups before term and at term, intact and ruptured membranes, latent phase and active phase of labor, respectively. 3. Before term, C-reactive protein levels of 0.8㎎/㎗ or higher and 2.0㎎/㎗ or higher were more frequent in the group of women in labor than those not in labor(23.64 vs. 4.69, p<0.001 and 12.73% vs. 3.13%, p<0.05, respectively), but those statistical differences were not seen between both group at term. Above results and review of literature suggest that serum C-reactive protein level of 2.0㎎/㎗ or higher may be reliable in early detection of infectious morbidity at term laboring women as well as laboring women before term, and the presence of subclinical infection should be suspected in the laboring women before term with serum C-reactive protein level of 0.8㎎/㎗ or higher.

지속성 외래 복막투석 환자에서 C반응단백, 맥압과 관상동맥질환의 관계

이정의 ( Jeong Ik Lee ),권순길 ( Soon Kil Kwon ),김상현 ( Sang Hyun Kim ),서장원 ( Jang Won Seo ),장재원 ( Jai Won Chang ),김순배 ( Soon Bae Kim ),이상구 ( Sang Koo Lee ),박정식 ( Jung Sik Park ) 대한신장학회 2003 Kidney Research and Clinical Practice Vol.22 No.1

목적 : 말기신부전으로 투석 중인 환자에서 C반응단백 (C-reactive protein, CRP)은 이환률과 사망률의 주요한 원인으로 알려진 관상동맥질환을 예견하는 지표로 알려져 있으며, 최근 여러 연구에서 맥압의 증가가 정상인에서 심혈관 질환과 사망률의 위험인자로 대두되고 있다. 따라서 본 연구는 복막투석 환자에서 이 두 가지 지표를 함께 측정하여 관상동맥질환의 위험도를 예견하는 지표로서의 가치를 알아보고자 시행되었다. 방법 : 복막투석을 시행하는 환자 중 thallium single photon emision computed tomography (thallium SPECT)를 시행하고 6개월 이상 추적관찰이 가능하였던 총 71명의 환자 (남자 42명, 여자 29명)를 대상으로 하였다. 나이, 성별, 흡연, 당뇨, 고혈압, 맥압, 체질량 지수에 대하여 조사하였으며 혈중 알부민, 총 콜레스테롤, 섬유소원, lipoprotein (a), C반응단백을 검사 하였다. 결과 : Thallium SPECT 양성군과 음성군으로 분류하여 비교한 결과 thallium SPECT 양성군에서 C반응단백, 맥압, 흡연율, 섬유소원의 값이 의의있게 높았다 (p<0.05). 맥압이 1 mmHg 증가시 관상동맥질환의 상대 위험도는 1.017배 (95% 신뢰구간 1.011-1.023), C반응단백이 0.01 mg/dL 증가시 관산동맥질환의 상대 위험도는 1.014배 (95% 신뢰구간 1.008-1.019) 증가하였다. 다증회귀분석 결과 C반응단백과 맥압이 심근 허혈에 영향을 주는 독립인자였다. Log C반응단백과 맥압간에는 양의 상관관계가 있었다. (p<0.01>. 결론 : 복막투석 환자에서 C반응단백과 맥압은 모두 관상동맥 질환을 예측하는데 유용한 지표로 이용할 수 있을 것으로 기대된다. 그러므로 복막투석 환자에서 뚜렷한 원인 없이 지속적으로 C반응단백과 맥압이 상승되어 있는 경우 허혈성 심질환에 대하여 검사가 이루어져야 할 것으로 생각된다. Background : Atheroclerotic vascular disease is the major cause of morbidity and mortality in dialysis patients. C-reactive protein (CRP) appears to be clinically useful in prediction of coronary heart disease. Elevated pulse pressure has been associated with an increased risk of cardiovascular disease among apparently healthy adults. Therefore, we evaluated the association between a persistent elevation of C-reactive protein level, pulse pressure and the presence of ischemic heart disease in patients with continuous ambulatory peritoneal dialysis (CAPD). Methods : A total of 71 CAPD patients (42 males 29 females) who underwent thallium SPECT and followed up more than 6 months were included. We collected the data about age, sex, smoking, diabetes, hypertension, pulse pressure and body mass index. Blood levels of albumin, total cholesterol, fibrinogen, lipoprotein (a) and C-reactive protein were measured. Results : The values of C-reactive protein, pulse pressure, smoking and fibrinogen were significantly higher in thallium SPECT positive group (p<0.05). A 0.01 mg/dL increase in C-reactive protein was associated with a 1.014 increase in the odds of having an elevated risk of coronary artery disease (95% confidence interval 1.008-1.019) and a 1 mmHg increase in pulse pressure was associated with a 1.017 increase in the odds of having an elevated risk of coronary artery disease (95% confidence interval 1.011-1.023). By multivariate logistic regression analysis, C-reactive protein and pulse pressure were independent risk factors for ischemic heart disease. Log CRP level was positively correlated with pulse pressure level (p<0.01). Conclusion : The baseline level of CRP and pulse pressure independently predicts the risk of coronary heart disease in CAPD patients. For patients who have a persistent elevation of CRP and pulse pressure without an apparent cause, we recommend a workup for ischemic heart disease.

조기양막파열 임부에서의 혈청 C-반응성 단백의 자궁내 감염의 분만 전 진단 및 신생아 이환 예측의 유용성에 관한 연구

최진(J Choi),윤보현(BH Yoon),신희철(HC Shin) 대한산부인과학회 1998 Obstetrics & Gynecology Science Vol.41 No.4

Objective: To investigate the diagnostic and prognostic performance of maternal serum C-reactive protein in patients with preterm premature rupture of membranes (PPROM) as the noninvasive method to detect the intrauterine infection. Study design: At admission, maternal blood was obtained for the determination of C-reactive protein in 120 patients with PPROM. Results: (1) Patients with acute histologic chorioamnionitis had significantly higher median C-reactive protein concentration (p<0.05).; (2) Patients with clinical chorioamnionitis had significantly higher median C-reactive protein concentration (p<0.0005).; (3) According to receiver operating characteristics (ROC) curve analysis, abnormal maternal C-reactive protein cut-off value was determined as more than 0.7 mg/㎗ and delivery outcome and neonatal morbidity were compared. Patients with the value of C-reactive protein more than 0.7 mg/㎗ had frequent premature delivery (p<0.00005), low birth weight (p<0.0001), frequent proven or suspected neonatal sepsis (p<0.05), intraventricular hemorrhage (p<0.05), brochopulmonary dysplasia (p<0.01), and necrotizing enterocolitis (p<0.0005). Conclusion: An elevated maternal serum C-reactive protein concentration identified patients with intrauterine infection and those at risk for adverse perinatal outcome. And the measurement of maternal serum C-reactive protein concentration was a good predictor of intrauterine infection and neonatal morbidity in patients with PPROM.

소아살모넬라장염에서 C-Reactive Protein 농도의 유용성

서정완 梨花女子大學校 醫科大學 醫科學硏究所 1997 EMJ (Ewha medical journal) Vol.20 No.1

1993년 10월에서 1996년 10월까지 장염으로 이대목동병원에 입원하여 살모넬라장염과 로타바이러스장염으로 진단된 환아 67명을 대상으로 임상증상과 C-Reactive protein, ESR, 혈액백혈구수 등을 비교 분석하여 다음과 같은 결론을 얻었다. 1) 장염으로 입원한 환아 67명 중 로타바이러스 장염군은 40명, 살모넬라 장염군 27명이었다. 로타바이러스 장염군의 평균연령이 유의하게 어렸다. 입원기간, 증상 발현 후 입까지의 기간, 입원당시의 체온, 구토횟수, 대변횟수도 두 군간의 유의한 차이가 없었다. 2) 탈수상태를 반영하는 입원시 소변의 비중은 로타바이러스장염군에서 유의하게 다소 높았다. 간기능수치는 두 군간에 유의한 차이가 없었다. 3) 살모넬라장염군에서도 대변내 잠혈반응은 14례(51.8%), 대변도말검사에서 백혈구는 19례(70.4%)에서만 검출되어 살모넬라장염과 로타바이러스장염을 감별하기 어려웠다. 상기도감염, 세균성감염, 열성결련 등의 동반질환이 있었던 증례는 로타바이러스장염군 19례(47.5%), 살모넬라장염군 7례(25.9%)로 로타바이러스장염군에서 동반증상이 유의하게 많았다. 4) 급성상 반응물질인 적혈구 침강소도(ESR), CRP(C-reactive protein)의 평균치는 살모넬라장염군에서 유의하게 높았다. ESR은 민감도에 비해 특이도가 지나치게 낮아 유용하지 않았다. 살모넬라장염에서 민감도는 C-RP≥0.5mg/dL에서 88.5%, 특이도는 C-RP≥0.8mg/dL에서 87.5%가 가장높았다. C-RP≥0.8mg/dL에서 민감도와 특이도를 같이 고려할 때 cut-off치로 C-RP≥0.8mg/dL가 가장 적당하였다. C-RP의 정량분석은 살모넬라장염을 조기에 진단하는데 유용하였다. Objectives : This study was performed to evaluate the usefulness of quantitative measurement of C-reactive protein(C-RP) and ESR in the differentiation between Rotaviral and Salmonella gastroenteritis in childern. Methods : On admission quantitative C-RP measurement by nephelometry, stool culture for slmonella and shigella, stool latex agglutination test for rotavirus(slidex rota-kit 2), stool occult blood, stool WBC and CBC with ESR were performed. Results : Of sixty seven children, 40(59.7%) children had Rotaviral gastroenteritis, and 27(40.3%) had Salmonella gastroenteritis. The C-RP levels and ESR of the patients with Salmonella gastroenteritis differed significantly from the Rotaviral gastroenteritis. At the level of 0.8 mg/dL, C-RP gave the best balance of sensitivity(84.6%) and specificity(87.5%). Conclusion : The quantitative measurement of C-RP levels may be a useful tool for predicting Salmonella gastroenteritis in children.

일반인구에서 자살사고와 혈장 C반응단백 농도의 연관성 : 국민건강영양조사

이정안,박선철,김경미,이봉주,박성우,서미경,이정구 대한우울조울병학회 2018 우울조울병 Vol.16 No.1

Objectives : The purpose of this study is to inquire into the relationship between the association of suicidal ideation and the plasma C-reactive protein level in the general population. Methods : This study selected a total of 5,090 subjects who have responded to the survey item on suicidal ideation status, and received the plasma C-reactive protein test, as a research subject using the 2015 data of the National Health and Nutrition Survey. This study conducted a covariance analysis by correcting a potential influence of demographic and hematological factors. Besides, this study intended to define the cut-off value of the optimum plasma C-reactive protein level, which once identified can distinguish between a subject with and without suicidal ideation using the model of the decision tree. Results : The Plasma C-reactive protein level of a subject having suicidal ideation was noted as being significantly higher than the one having no suicidal ideation in the covariance analysis (p=0.046). In addition, the proper cut-off value of the plasma C-reactive protein level between the subjects with and without suicidal ideation was found to be 1.30 mg/L (p=0.003). Conclusion : It is noted that the high plasma C-reactive protein level showed a significant correlation with individuals with noted suicidal ideation. In addition, this study has significance in that it presented that the plasma C-reactive protein concentration has the possibility as an accurate and significant marker of suicidal ideation in the general population.

Molecular Chaperonic Function of C-Reactive Protein Induced by Heating in HT-29 Human Colon Carcinoma Cells

Lee, Soo-Young,Jung, Hyun-Jung,Kim, Hyun-Soo,Lee, Seung-Chul,Lee, Si-Back,Joe, Jae-Hoon,Kim, You-Mie Korean Society for Biochemistry and Molecular Biol 2000 Journal of biochemistry and molecular biology Vol.33 No.5

The effects of heat shock, or all-trans retinoic acid, on the expression of the C-reactive protein mRNA in the HT-29 human colon carcinoma cells, as well as the functional role of the C-reactive protein as a molecular chaperone, were studied. The expression level of the C-reactive protein mRNA in the HT-29 cells was increased time-dependently when exposed to heat-shock, and dose-dependently when treated with all-trans retinoic acid. The activities of transglutaminase C and K in the HT-29 cells were significantly increased when treated with all-trans retinoic acid. The C-reactive protein prevented thermal aggregation of the citrate synthase and stabilized the target enzyme, citrate synthase. The C-reactive protein promoted functional refolding of the urea-denatured citrate synthase up to 40-70%. These results suggest that the C-reactive protein, which is induced in human colon carcinoma cells, when heated or treated with all-trans retinoic acid has in a part functional activity of the molecular chaperone.

The prognostic value of procalcitonin, C-reactive protein and cholesterol in patients with an infection and multiple organ dysfunction

Siarhei Anatolevich Tachyla,Alexey Viktorovich Marochkov,Artur Leonidovich Lipnitski,Yulia Gennadevna Nikiforova 대한마취통증의학회 2017 Korean Journal of Anesthesiology Vol.70 No.3

Background: To establish the prognostic value of procalcitonin, C-reactive protein and cholesterol levels for mortality in patients with an infection and multiple organ dysfunction. Methods: A prospective case-control study was performed, including 67 patients admitted to the intensive care unit with an infection and multiple organ dysfunction in whom cholesterol, procalcitonin, and C-reactive protein levels were measured on admission and during the course of treatment. Results: The associations between in-hospital mortality and procalcitonin, C-reactive protein, and cholesterol levels were analyzed. Logistic regression analysis showed that cholesterol (odds ratio [OR], 1.858; 95% CI, 1.170–2.949; P = 0.009) and C-reactive protein (OR, 4.408; 95% CI, 2.019–9.624; P < 0.001) levels were predictors of mortality. A receiver operating characteristic curve analysis yielded an area under the curve (AUC) of 0.774 and 95% CI of 0.693–0.855 (P < 0.001) for C-reactive protein, an AUC of 0.66 and 95% CI of 0.535–0.784 (P = 0.019) for procalcitonin, and an AUC of 0.654 and 95% CI of 0.593–0.715 (P < 0.001) for cholesterol as predictors of mortality. When combined with the bioscore system for mortality, these markers yielded an AUC of 0.845 and 95% CI of 0.770–0.921 (P < 0.001), with sensitivity of 89.1% and specificity of 83.1%. Conclusions: The combination of procalcitonin, C-reactive protein, and cholesterol levels in a single scoring system yielded high predictive value for mortality.

건강증진센터를 내원한 성인에서 혈청 페리틴과 고감도 C-반응 단백과의 관련성

오은실,신현식,이지원,임지애,이덕철,이혜리 대한가정의학회 2008 Korean Journal of Family Medicine Vol.29 No.12

Background: More attention is given to oxidative hypothesis which causes atherosclerosis to be recognized as inflammatory response. The relationship between serum ferritin which catalyzes lipid peroxidation and high sensitivity C-reactive protein which reflects vascular inflammation was investigated among adults in a health promotion center. Methods: The study group consisted of 297 men and women (men 86, women 211) who visited the health promotion center of a hospital in Seoul to have a health checkup from October 1, 2004 to April 1, 2005. These subjects answered the questionnares and were measured in the following; blood tests, brachial-ankle pulse wave velocity and several anthropometric measurements. Statistical analysis was performed on 111 subjects after exclusion of those subjects who were taking antihypertensive agents or antidiabetic agents, and who had acute inflammatory diseases, acute liver diseases, anemia, and who had a WBC ≥11,000×103/mm3 or a serum ferritin ≥200 ug/L or a ABI (Ankle Brachial Index) <0.9. Results: The average serum ferritin concentration of men against women was 132.57±43.12 ng/ml to 78.23±38.10 ng/ml which means that men have about 1.7 times as high concentration than women (P<0.001). Serum ferritin was significantly correlated with high sensitivity C-reactive protein (r=0.332). Even in multiple stepwise regression analysis, there was a independent relationship between serum ferritin and high sensitivity C-reactive protein (β=0.138, P=0.010). When we analyzed with distinction of sex, this relationship in women was constant (β=0.131, P=0.031), but serum ferritin in men just showed the trend of correlation with BMI (β=9.510, P=0.059). Conclusion: There is a significant relationship between the increase of serum ferritin and high sensitivity C- reactive protein in healthy women; furthermore, studies in men need to be confirmed. 연구배경: 최근 죽상경화를 염증반응으로 인식하면서 이를 일으키는 산화가설에 주목하고 있다. 이에 건강증진센터를 내원한 성인을 대상으로 지질 과산화를 촉매하는 것으로 생각되어지는 혈청 페리틴과 혈관염증을 반영하는 대표적인 인자인 고감도 C-반응 단백과의 관련성을 알아보고자 한다. 방법: 2004년 10월 1일부터 2005년 4월 1일까지 일개 종합병원 건강증진센터 20세 이상 수진자 297명을 대상으로 설문조사를 시행하고 신체계측, 혈액 검사, 혈압 및 상완-발목 맥파속도를 측정하였다. 그 중 고혈압, 고지혈증, 당뇨병으로 치료중인 환자, 백혈구 11,000×103/mm3이 넘거나, 혈청 페리틴 200 ug/L이 넘는 경우, 급성 감염성 질환자, 고감도 C-반응 단백이 10 mg/L를 초과하는 경우, 급성 간 질환자, 빈혈 해당자 및 ABI (Ankle Brachial Index) 0.9 미만인 군을 제외한 111명을 연구대상으로 하였다. 결과: 혈청 페리틴 농도는 남자에서 131.57±43.12 ng/ml, 여자에서 78.23±38.10 ng/ml로 남자가 여자보다 약 1.7배 높았다(P<0.001). 혈청 페리틴은 상관분석에서 체질량지수(r=0.227), 고밀도지단백(r=−0.189) 및 고감도 C-반응 단백(r=0.332)과 상관성을 보였으며, 단계적 다중회귀분석에서도 고감도 C-반응단백과 독립적인 관련성이 있었다(β=0.138, P=0.010). 또한 성별을 나누어 분석하였을때, 여성에서 혈청 페리틴은 여전히 고감도 C-반응 단백과 독립적인 관련성이 지속되었으나 (β=0.131, P=0.031), 남성에서는 체질량 지수만이 혈청 페리틴과 상관성을 보이는 정도였다(β=9.510, P=0.059). 결론: 건강한 성인 여성에서 혈청 페리틴의 증가는 심혈관계 질환의 예측인자인 고감도 C-반응 단백과 독립적인 관련성을 보였으며, 남성에 대해 결론을 내리기 위해서는 추후 연구가 필요하다.

Feeding Soy Protein Concentrates with Low and High Isoflavones Alters 9 and 18 Weeks Serum Isoflavones and Inflammatory Protein Levels in Lean and Obese Zucker Rats

Wei Li,Nathan C. Twaddle,Beverly Spray,Bernice Nounamo,Behjatolah Monzavi-Karbassi,Reza Hakkak 한국식품영양과학회 2023 Journal of medicinal food Vol.26 No.2

Soy's anti-inflammatory properties contribute to the health benefits of soy foods. This study was designed to investigate the bioavailability of soy isoflavones and whether the isoflavone content of soy protein concentrate diet would affect serum inflammatory proteins in an obese (fa/fa) Zucker rat model. Six-week-old male lean (L) and obese (O) Zucker rats were fed a casein control diet (C), soy protein concentrate with low isoflavones (SPC-LIF), or soy protein concentrate with high isoflavones (SPC-HIF) (7 rats/dietary group) before being killed at 9 and 18 weeks. Serum samples were analyzed for isoflavones and inflammatory proteins. At both time points, serum total (aglycone + conjugates) genistein, daidzein, and equol concentrations were significantly higher in L-SPC-HIF and O-SPC-HIF groups compared with L-SPC-LIF and O-SPC-LIF groups, respectively, and were not detectable in either L-C or O-C groups. At week 9, serum C-reactive protein (CRP) concentration was significantly lower in O-SPC-HIF group compared with O-C and O-SPC-LIF group, whereas proteins tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) levels did not differ between any groups. At week 18, serum CRP levels in both O-SPC-HIF and O-SPC-LIF groups were significantly lower compared with the O-C group. TNF-α level was higher in the O-SPC-LIF group compared with both O-C and O-SPC-HIF groups, whereas IL-6 levels were not different between any groups. Taken together, feeding Zucker rats SPC-LIF and SPC-HIF diets led to different serum isoflavone concentrations in both L and O Zucker rats and altered CRP and TNF-α levels in obese Zucker rats compared with controls.

Dose-dependent Effects of Dietary Folate on Aortic Relaxation and Hepatic C-reactive protein Levels in C57BL/6 Mice

Eunhee Kong(공은희),Syeda T. Hasan(하산시다),Hyeran Jang(장혜란),Ella M. Zimmerly(짐머리엘라),Sang-Woon Choi(최상운),Mohsen Meydani(메이다니모슨) 한국생명과학회 2015 생명과학회지 Vol.25 No.8

혈관내피세포 기능장애는 동맥경화증 발생의 초기단계이다. 비타민B군(B6, B12, 엽산)은 혈관항상성에 중요한 인자이다. 이들 비타민B군이 결핍되면 혈관항상성에 변화가 생겨 심혈관질환을 유발한다. 비타민B군 중 엽산은 내피세포에서 산화질소 항상성에 중요한 역할을 한다. 동맥경화증에 관련된 섭취엽산의 용량의존적 효과를 알기위해, C57BL/6 쥐의 대동맥이완과 간 C반응단백질 수준을 연구하였다. 본 연구는 총 54마리의 C57BL/6 쥐를 서양식이군과 대조식이군으로 나누고, 각각은 다시 엽산섭취용량(0.2, 2, 8mg/kg)에 따라 3형태의 하위집단으로 나누었다. 18개월 동안의 식이섭취후, 양군의 8mg/kg 엽산섭취용량에서는 대동맥 이완반응을 전혀 나타내지 않았고 양군의 차이도 없었다(p<0.05). 모든 엽산섭취용량(0.2, 2, 8mg/kg)에서 간 C반응단백질 수준은 대조식이군보다 서양식이군에서 더 높았다(p=0.035). 양군의 간 C반응단백질 수준은 0.2 mg/kg 엽산섭취용량에서 가장 낮았다(p<0.05). 결론적으로, C57BL/6 쥐에서 식이에 관계없이 0.2 mg/kg 엽산섭취용량만으로도 대동맥의 이완반응을 유도하고 간 C반응단백질 수준을 낮춤으로써 동맥경화증을 충분히 예방할 수 있다. Endothelial dysfunction is an initial step in atherosclerosis. B vitamins (B6, B12, and folate) are important contributing factors to vascular homeostasis. Deficiencies in these B vitamins induce cardiovascular diseases by altering vascular homeostasis. Folate plays important roles in nitric oxide homeostasis in the endothelium. To determine the dose-dependent effect of dietary folate on atherosclerosis, we studied aortic relaxation and hepatic C-reactive protein (CRP) levels in C57BL/6 mice. In this study, a total of 54 male C57BL/6, 8-wk old mice were split into 2 dietary groups (control and Western style diet). Each diet group was divided into 3 subgroups according to dietary folate dosage (0.2, 2, and 8 mg/kg). After 18 months, the relaxation response seen in aortic rings from mice fed 0.2 or 2 mg folate/kg in both diet groups. However, the aortic relaxation response was not seen and no differences were observed in mice fed 8mg folate/kg in either diet group (p<0.05). Hepatic CRP levels at all folate dosages (0.2, 2, 8 mg folate/kg) were higher in the groups fed a Western style diet than in mice fed a control diet (p=0.035). CRP levels were lower in mice fed 0.2 mg folate/kg than in mice fed 2 or 8 mg folate/kg in both diet groups (p<0.05). These results indicate that in C57BL/6 mice 0.2 mg folate/kg may be enough to prevent atherosclerosis by inducing the relaxation responses of the aorta and by reducing levels of hepatic CRP, regardless of dietary style.