배추 ‘춘광’의 저장 중 1-MCP 처리효과

홍세진,박남일,김병삼,엄향란 한국원예학회 2018 원예과학기술지 Vol.36 No.2

배추의 수급조절을 위해서는 저온저장이 요구된다. ‘춘광’ 배추의 저장성 향상을 위한 방안으로 저장 중 1-methylcyclopropene (1-MCP) 처리효과를 확인하였다. 수확된 배추는 즉시 실험실로 옮겨와 4개의 그룹(차압예냉, 차압예냉 + 0.03mm LLDPE Liner, 차압예냉 + 2μL·L-1 1-MCP 처리구, 차압예냉 + 2μL·L-1 1-MCP + 0.03mm LLDPE Liner 처리구)으로 분류하여 각각의 처리를 실시한 후 2°C, 95% RH 저온에서 저장하였다. 저장 중 품질변화는 무게 감모율, 가용성 고형물 함량, 경도, 1-aminocyclopropane carboxylic acid oxidase(ACO), ethylene receptor(ETR), 그리고 polygalacturonase(PG) 유전자 발현을 통해 확인하였다. 저장기간 동안 각 실험구의 감모율은 Liner 처리의 유무에 의해서 현저한 차이를 보였다. 반면 1-MCP 처리 유무에 따른 감모율에는 차이가 없었다. 가용성 고형물의 함량은 수확 시에 2.3°Brix였으며, 저장기간 전반에 걸쳐 모든 처리구에서 2.4-3.0°Brix 범위에 포함되었다. 각 처리구들은 통계적으로는 유의적 차이를 보이나 값은 0.6°Brix 내외로 저장기간 동안 가용성 고형물의 증감에 따른 경향도 처리 간에 유사하였다. 배추의 경도는 수확 직후 25.5N이었으며, 저장기간 동안 경도의 증감을 보이나 처리 간에 뚜렷한 경향은 없었다. 1-MCP 처리는 배추를 저장하는 하는 동안 ‘춘광’ 배추의 외관상 품질에는 영향을 미치지 않았으므로 1-MCP가 배추의 숙성에 관여하는 유전자 발현과의 상관성에 대해서 확인하였다. 에틸렌 생합성에 관여하는 ACO와 ETR 유전자들의 발현 정도를 확인하였으나 1-MCP 처리 유무에 따른 유전자 발현의 차이가 보이지 않았다. 또한 세포벽 분해효소인 PG 유전자 발현 정도에도 1-MCP 처리는 유의적 차이가 없었다. 이상의 결과에서 1-MCP 처리는 배추저장에는 효과가 미비하다고 판단된다. 하지만 저장기간 동안 ‘춘광’ 품종 배추의 무게손실을 최소화 하기 위해서는 PE film을 이용한 Liner 처리가 반드시 요구되며 이를 통해 처리 후 9주까지 품질유지가 가능하였다. Kimchi cabbage needs cold storage for regulation of supply and demand. Postharvest application of 1-methylcyclopropene (1-MCP) in the storability of Kimchi cabbage under cold storage condition was investigated. Kimchi cabbage (Brassica campestris L. ‘Choongwang’) was divided four groups, forced air cooling (FAC), FAC + 0.03 mm linear low density polyethylene (LLDPE) Liner, FAC + 2 μL·L-1 1-MCP, and FAC + 2 μL·L-1 1-MCP + 0.03 mm LLDPE Liner. After each treatment Kimchi cabbage was stored at 2°C, 95% RH. Quality parameters were weight loss, soluble solids content (SSC), firmness, and gene expression of 1-aminocyclopropane carboxylic acid oxidase (ACO), ethylene receptor (ETR), and polygalacturonase (PG). Weight loss during storage was showed significant difference by Liner treatment, but was not influenced by 1-MCP treatment. SSC of Kimchi cabbage was 2.3°Brix at harvest and all groups included to 2.4-2.9°Brix range during storage. Although in each group SSC was showed difference during storage, the difference was approximately less than 0.5°Brix. In general, firmness was 25.5 N at harvest and slightly increased or decreased during storage in all groups. However, there was no tendency found among groups. Also ripening related gene expressions, such as ACO, ETR, and PG, were investigated. 1-MCP treatment was not significant difference among all roups about ethylene biosynthesis genes (ACO, ETR) and softening related gene (PG). These data show that 1-MCP treatment was not recommended for Kimchi cabbage storage. However Liner treatments such as PE film necessarily need tominimize weight loss of Kimchi cabbage ‘Choongwang’ during cold storage for 9 weeks.

순무(Brassica campestris ssp rapa) 뿌리의 화학성분

최연희,김정숙,서지희,이종원,김영섭,유시용,강종성,김영균,김성훈 한국생약학회 2004 생약학회지 Vol.35 No.3

Twelve constituents were isolated from the MeOH extract of the root of Brassica campestris L. ssp rapa. They were identified as linoleic acid methylester (1), palmitic acid (2), b-sitosterol (3), 1-methoxyindole-3-acetonitrile (4), indole-3-acetonitrile (5), linolenic acid (6), goitrin (7), 4-hydroxycinnamyl alcohol (8), coniferyl alcohol (9), p-coumaroylglucose (11) and feruloylglucose (12), on the basis of spectral data respectively.

Molecular Cloning and Sequence Analysis of L-galactono-1,4-lactone Dehydrogenase Gene cDNA from Brassica campestris ssp. chinensis

Xiliang Ren,Xilin Hou 한국원예학회 2006 한국원예학회 기타간행물 Vol.- No.-

Growth and Physiological Responses of Chinese Cabbage and Radish to Long-term Exposure to Elevated Carbon Dioxide and Temperature

CHOIEUN YOUNG,서태철,이상규,조일환,James Stangoulis 한국원예학회 2011 Horticulture, Environment, and Biotechnology Vol.52 No.4

Future forecasts for climate change predict the global mean surface air temperature rise by 1 - 4℃ and double current atmospheric CO2 level before the end of 21 century. Increased atmospheric temperature and CO2 concentration are particularly important concerns for agricultural, horticultural and native plant production. In this study, effects of long-term exposure to elevated temperature and carbon dioxide (CO2) on the growth and physiological responses of 3 cultivars of Chinese radish (Raphanus sativus L.) and 3 cultivars of Chinese cabbage (Brassica campestris L.) were examined. In result,the radishes exposed to elevated CO2 for 90 days after sowing (DAS) resulted in little or no change in the root dry weights and the rate of photosynthesis compared with those grown in ambient levels of CO2. In contrast, long-term exposure to elevated CO2 in cabbage had variable effects on the leaf dry weight. As a result of acclimating to the elevated temperature, the radish ‘Chunha’ had a higher rate of photosynthesis, stomatal conductance and internal CO2 concentration than in the control condition. Furthermore, the long-term exposure to a combination condition of elevated temperature and CO2 increased root dry weights of the radishes ‘Cheongdae’ and ‘Chunha’ more than elevated temperature alone. The combination of elevated CO2and temperature stimulated the growth of roots more than that of shoots in the radish ‘Chunha’, and thus may have led a higher rate of nutrient uptake than other radish cultivars. In contrast, when the cabbage ‘Chun-gwang’ was exposed to a combination of elevated temperature and CO2 for 90 DAS, the leaf dry weight decreased about 3-fold more than that only exposed to elevated CO2 with drastic decreases in stomatal conductance, internal CO2 and photosynthesis rate. When the cabbage ‘Samjin’ was exposed to either elevated temperature alone or both elevated temperature and CO2 for 80 DAS, the decrease in the leaf dry weight was less than that of the other cabbage cultivars. Results indicated that the radish ‘Chunha’ and the cabbage ‘Samjin’ tolerated either elevated temperature alone or combination condition of elevated temperature and CO2 more than other cultivars.

Growth and Physiological Responses of Chinese Cabbage to Different Light Intensity Until Leafy Head Formation

In-Chang Son,Kyung Hwan Moon,Eun Young Song,Seung Hwan Wi,Hyeong-Ho Seo,Young Eel Moon,Vangimalla Reddy,Jinyoung Yang,Richard Sicher,Soonja Oh 한국원예학회 2018 원예과학기술지 Vol.36 No.2

The effects of light on the growth, leaf morphology, physiological responses, and quality of Chinese cabbage (Brassica campestris L. ssp. pekinensis) were examined at five light intensity levels (200-1,000 μmol·m<SUP>-2</SUP>·s<SUP>-1</SUP>) to determine the optimal light intensity for the production of high-quality cabbage heads. In each light treatment, peak irradiance values at midday were 200 (I), 400 (II), 600 (III), 800 (IV), and 1,000 (V) μmol·m<SUP>-2</SUP>·s<SUP>-1</SUP>. Fresh and dry weights of plant shoots significantly increased with light intensity; leaf number and area were positively correlated with light intensity, although the total and mean leaf area for plants in group IV exceeded that of group V. The leafy head formation was observed in high light intensity (800 and 1,000 μmol·m<SUP>-2</SUP>·s<SUP>-1</SUP>), but not in the low light intensity conditions. Total photosynthetic capacity in Chinese cabbages grown under 800 μ mol·m<SUP>-2</SUP>·s<SUP>-1</SUP> increased due to the greater leaf area of the plants, even though the photosynthetic rate was lower than that measured under 1,000 μmol·m<SUP>-2</SUP>·s<SUP>-1</SUP>. In addition, the values of F<SUB>v</SUB>/F<SUB>m</SUB> indicated that Chinese cabbage was mildly stressed under 1,000 μmol·m<SUP>-2</SUP>·s<SUP>-1</SUP>. We recommend a light intensity of 800 μmol·m<SUP>-2</SUP>·s<SUP>-1</SUP> for the production of high-quality Chinese cabbage. We hope that this study can help emphasize the importance of light intensity when maximizing the economic benefits of Chinese cabbage.

Can Commercial Bee Attractants Influence in Increasing Bee Pollination and Productivityof Mustard, Brassica campestris L.?

V. Sivaram,K. V. Jayaramappa 한국양봉학회 2013 韓國養蜂學會誌 Vol.28 No.2

Honeybees are considered as most efficient pollinators in the ecosystem and utilization of bees as pollinators for various agri-horticultural crops is of great significance in increasing crop productivity through pollination. The different concentrations of Bee-Q and Fruit boost<SUP>TM</SUP>? was evaluated to understand the honeybee visitation pattern of target crop for improving pollination efficiency. The bee visitations on Mustard flowers were observed for two weeks followed by estimation of seed yield. The observations indicate that, Bee-Q at 12.5g/l and Fruit boost at 0.75ml/l of Mustard plots significantly attracted highest number of bee foragers than the control plots. The present investigation suggests that the bee attractants increase marginal percentage of bee visitation, seed yield and germination percentage of Mustard.

Characterization of a stamen-specific cDNA encoding a novel plant defensin in Chinese cabbage

Park, Hyeong-Cheol,Kang, Yun-Hwan,Chun, Hyun-Jin,Koo, Ja-Choon,Cheong, Yong-Hwa,Kim, Cha-Young,Kim, Min-Chul,Chung, Woo-Sik,Kim, Jong-Cheol,Yoo, Jae-Hyuk,Koo, Yoon-Duck,Koo, Sung-Chul,Lim, Chae-Oh,Lee Plant molecular biology and biotechnology research 2002 Plant molecular biology and biotechnology research Vol.2002 No.-

We isolated a stamen-specific cDNA, BSD1(Brassica stamen specific plant defensin 1)that encodes a novel plant defensin peptide in Chinese Chinese cabbage (Brassica campestris L. ssp. pekinensis). Plant defensins are antimicrobial peptides containing eight highly conserved cysteine residues linked by disulfide bridges. In BSD1, the eight cysteine residues and a glutamate residue at position 29 are conserved whereas other amino acid residues of the plant defensins consensus sequence are substituted. BSD1 transcripts accumulate specifically in the stamen of developing flowers and its level drops as the flowers mature. The recombinant BSD1 produced in Escherichia coli showed antifungal activity against several phytopathogenic fungi. Furthermore, constitutive over-expression of the BSD1 gene under the control of the cauliflower mosaic virus (CaMV) 35S promoter conferred enhanced tolerance against the Phytophthora parasitica in the transgenic tobacco plants.

Pallet Unit MAP 포장과 플라즈마 복합처리가 봄배추의 선도 연장에 미치는 효과

이혜옥(Hye-Ok Lee),이영주(Young-Joo Lee),김지영(Ji-Young Kim),김병삼(Byeong-Sam Kim) 한국원예학회 2018 원예과학기술지 Vol.36 No.2

This study investigated the effects of the application of pallet unit modified atmosphere packaging (MAP) and plasma on the freshness of spring Kimchi cabbage ‘Chungwang’. Kimchi cabbage were cultivated and harvested in Pyeongchang, Gangwondo, Korea, placed in plastic boxes, and precooled at 8°C for 24 hours. Kimchi cabbages were then kept in pallet units and stored in one of two low temperature conditions at 1 ± 0.5°C; one with a plasma generator installed and the other without plasma (control). Kimchi cabbages pretreated after harvest were divided into four groups and placed into boxes covered with one of four types of packaging: PVC wrap, LDPE film (Ø 20 mm punched), newspaper, or no packaging. Kimchi cabbages in the control group lost 13% of their weight during the storage period, while those in all three of the treatment groups lost less than 7% during the same period, thus demonstrating suppressed weight loss. Kimchi cabbages in the control group exhibited 10-19% trimming loss during the storage period. The plasma-treated group without any covering lost less than the control group, thus it is presumed that plasma treatment somewhat affects storage. The plasma-treated group covered with LDPE film after applying moisture absorption paper lost less than 9% until 9 weeks after storage. In the 12th week after storage, it showed about 13% trimming loss the lowest of all the treated groups. As a result of thise sensory evaluation, most of the treated groups lost their marketability at 3-6 weeks after storage, but the treated group covered with LDPE film after applying moisture absorption paper and stuffing the product scored more than 7 points - 6 points is the minimum acceptable score for cabbage suitable for making kimchi, thus this proves that spring Kimchi cabbage can be stored for a long time. We conclude that the combination of precooling, moisture absorption paper, packaging with LDPE film in a pallet unit, and the use of plasma, can extend the storability of spring Kimchi cabbage.

춘화, 온도와 토양건조 기간에 따른 배추의 생장 및 수량

이상규(Sang Gyu Lee),이희주(Hee Ju Lee),김성겸(Sung Kyeom Kim),최장선(Chang Sun Choi),박성태(Sung Tae Park),장윤아(Yoon Ah Jang),도경란(Kyung Ran Do) 한국원예학회 2015 원예과학기술지 Vol.33 No.6

This study was carried out to determine the effects of vernalization, temperature, and soil water deficit (SD) on mesophyll cells, growth, and yield of Chinese cabbage (Brassica campestris L). The palisade parenchyma and spongy tissues of Chinese cabbage were observed under full irrigation and two weeks of SD treatment. These cells were severely collapsed by four weeks SD treatment. The SD treatment had the greatest influence on the growth of Chinese cabbage among the tested treatment factors (vernalization, temperature, and SD), growth significantly decreased by severe drought treatment (four weeks SD treatment). In addition, the relative growth rate, unit leaf rate, leaf area ratio, specific leaf area, and leaf weight ratio were significantly affected by SD treatment; however, other individual factors and their combined treatments did not influence the analyzed growth parameters. The yield under vernalization after high temperature and full irrigation treatments was 3,056 ㎏/10 a, which was the greatest among all the tested treatments, while four-week SD treatment significantly reduced the yield. Head formation of Chinese cabbage was not altered under SD treatment, and vernalization treatments did not induce bolting. Our results indicated that collapsing mesophyll cells and reduced growth and yield were induced by SD treatment. Thus we suggest that optimal irrigation system should be install to avoid or overcome crippling drought conditions in the open field.

Variation in Bioaccumulation of Chinese Cabbage according to the Concentration and Frequency of Tetracycline Exposure

Hadjer Chohra,Young-Nam Kim,Mi Sun Cheong,Yong Bok Lee 한국토양비료학회 2022 한국토양비료학회 학술발표회 초록집 Vol.2022 No.10