Overexpression of the MYB transcription factor MYB28 or MYB99 confers hypersensitivity to abscisic acid in arabidopsis

Yong-Tao Yu,Zhenwu Zhu,Kai Lu,Chao Bi,Shan Liang,Xiao-Fang Wang,Da-Peng Zhang 한국식물학회 2016 Journal of Plant Biology Vol.59 No.2

The phytohormone abscisic acid (ABA) plays a vital role in plant development and response to abiotic stress, and many components in ABA signaling remain to be discovered to fully understand the highly complex ABA signaling network. Here we reported that over-expression of MYB28 or MYB99, two R2R3 MYB transcription factors in Arabidopsis thaliana, confers hypersensitivity to exogenous ABA during seed germination, cotyledon greening and early seedling growth. However, both myb28-1 and myb99-1 knockout mutants exhibit wild-type ABA phenotypes most likely due to a functional redundancy of the multiple MYB members. Real-time PCR analysis shows that over-expression of the MYB28 or MYB99 changes expression of a subset of ABA-responsive genes. Collectively, these findings suggest that MYB28 and MYB99 play important, positive roles in ABA signaling during seed germination and early seedling growth.

On the norm of the operator $aI+bH$ on $L^p(\mathbb R)$

Yong Ding,Loukas Grafakos,Kai Zhu 대한수학회 2018 대한수학회보 Vol.55 No.4

We provide a direct proof of the following theorem of Kalton, Hollenbeck, and Verbitsky \cite{HKV}: let $H$ be the Hilbert transform and let $a,b$ be real constants. Then for $1<p<\infty$ the norm of the operator $aI+bH$ from $L^p(\mathbb R)$ to $L^p(\mathbb R)$ is equal to $$ \bigg(\max_{x\in \mathbb R}\frac{|ax-b+(bx+a)\tan \frac{\pi}{2p}|^p+|ax-b-(bx+a)\tan \frac{\pi}{2p}|^p}{|x+\tan \frac{\pi}{2p}|^p+|x-\tan \frac{\pi}{2p}|^p} \bigg)^{\frac 1p}. $$ Our proof avoids passing through the analogous result for the conjugate function on the circle, as in \cite{HKV}, and is given directly on the line. We also provide new approximate extremals for $aI+bH$ in the case $p>2$.

ON THE NORM OF THE OPERATOR aI + bH ON L^p(ℝ)

Ding, Yong,Grafakos, Loukas,Zhu, Kai Korean Mathematical Society 2018 대한수학회보 Vol.55 No.4

We provide a direct proof of the following theorem of Kalton, Hollenbeck, and Verbitsky [7]: let H be the Hilbert transform and let a, b be real constants. Then for 1 < p < ${\infty}$ the norm of the operator aI + bH from $L^p(\mathbb{R})$ to $L^p(\mathbb{R})$ is equal to $$\({\max_{x{\in}{\mathbb{R}}}}{\frac{{\mid}ax-b+(bx+a){\tan}{\frac{\pi}{2p}}{\mid}^p+{\mid}ax-b-(bx+a){\tan}{\frac{\pi}{2p}}{\mid}^p}{{\mid}x+{\tan}{\frac{\pi}{2p}}{\mid}^p+{\mid}x-{\tan}{\frac{\pi}{2p}}{\mid}^p}}\)^{\frac{1}{p}}$$. Our proof avoids passing through the analogous result for the conjugate function on the circle, as in [7], and is given directly on the line. We also provide new approximate extremals for aI + bH in the case p > 2.

Self‐assembly of covalent porphyrin compound and its enhanced electrochemiluminescence performance

Wen-Kai Zhu,Wen-Rong Cai,Zhen-Zhi Yin,Ming-Jie Cheng,Kong Yong 대한화학회 2022 Bulletin of the Korean Chemical Society Vol.43 No.12

A novel Zn-coordination covalent porphyrin assembly (TCPP-BZA-Zn) is designed. The assembly structure is synthesized through the amidation reac- tion between the porphyrin terminal carboxyl group and the amino group of benzylamine (BZA), and further assembled through π–π stacking. In particular, the inherently ordered structure of TCPP-BZA-Zn with Zn as the catalytic active center endows the porphyrin assembly structure with several obvious advantages, such as high ion transport properties and high electrocatalytic per- formance. In the presence of hydrogen peroxide as a co-reaction reagent, TCPP-BZA-Zn/GCE showed excellent ECL behavior. The amplification phenome- non of ECL was further studied by cyclic voltammetry and the corresponding mechanism was proposed. Based on TCPP-BZA-Zn, an electrochemilumines- cence sensor was constructed for copper ion detection. The ECL intensity of the sensor shows a good linear relationship with the concentration of copper ion in the range of 10 nM–1 mM, and the detection limit is 1.3 nM.

Degradation kinetics of vitamins in premixes for pig: effects of choline, high concentrations of copper and zinc, and storage time

Yang Pan,Wang Hua Kai,Zhu Min,Li Long Xian,Ma Yong Xi 아세아·태평양축산학회 2021 Animal Bioscience Vol.34 No.4

Objective: The present work was undertaken to evaluate the effects of storage time, choline chloride, and high concentrations of Cu and Zn on the kinetic behavior of vitamin degradation during storage in two vitamin premixes and four vitamin-trace mineral (VTM) premixes. Methods: Two vitamin premixes (with or without 160,000 mg/kg of choline) were stored at 25°C and 60% humidity. Besides, four VTM premixes were used to evaluate the effects of choline (0 vs 40,000 mg/kg) and trace minerals (low CuSO4+ZnO vs high CuSO4+ZnO) on vitamin stability in VTM premixes stored in room, and the VTM premixes were stored in room temperature at 22°C. Subsamples from each vitamin and VTM premix were collected at 0, 1, 2, 3, 6, and 12 months. The retention of vitamin A (VA), vitamin D3 (VD3), vitamin E (VE), vitamin K3 (VK3), vitamin B1 (VB1), vitamin B2 (VB2), vitamin B3 (VB3), vitamin B5 (VB5), and vitamin B6 (VB6) in vitamin premixes and VTM premixes during storage was determined. The stability of vitamins in vitamin premixes and VTM premixes was determined and reported as the residual vitamin activity (% of initial) at each sampling point. Results: The effect of choline on VK3 retention was significant in vitamin premixes (p<0.05). The negative effect of storage time was significant for the retentions of VD3, VK3, VB1, VB2, VB5, and VB6 in vitamin premix (p<0.05). For VTM premixes, negative effect of storage time was significant (p<0.05) for the losses of vitamin in VTM premixes. Choline and high concentrations of Cu and Zn significantly increased VA, VK3, VB1, and VB2 loss during storage (p<0.05). The supplementation of high concentrations of Cu and Zn significantly decreased the concentrations of VD3 and VB6 (p<0.05) in VTM premixes at extended storage time. Conclusion: The maximum vitamin stability was detected in vitamin and VTM premixes containing no choline or excess Cu and Zn. The results indicated that extended storage time increased degradation of vitamin in vitamin or VTM premixes. These results may provide useful information for vitamin and VTM premixes to improve the knowledge of vitamin in terms of its stability. Objective: The present work was undertaken to evaluate the effects of storage time, choline chloride, and high concentrations of Cu and Zn on the kinetic behavior of vitamin degradation during storage in two vitamin premixes and four vitamin-trace mineral (VTM) premixes.Methods: Two vitamin premixes (with or without 160,000 mg/kg of choline) were stored at 25°C and 60% humidity. Besides, four VTM premixes were used to evaluate the effects of choline (0 vs 40,000 mg/kg) and trace minerals (low CuSO₄+ZnO vs high CuSO₄+ZnO) on vitamin stability in VTM premixes stored in room, and the VTM premixes were stored in room temperature at 22°C. Subsamples from each vitamin and VTM premix were collected at 0, 1, 2, 3, 6, and 12 months. The retention of vitamin A (VA), vitamin D₃ (VD₃), vitamin E (VE), vitamin K₃ (VK₃), vitamin B₁ (VB₁), vitamin B₂ (VB₂), vitamin B₃ (VB₃), vitamin B₅ (VB₅), and vitamin B₆ (VB₆) in vitamin premixes and VTM premixes during storage was determined. The stability of vitamins in vitamin premixes and VTM premixes was determined and reported as the residual vitamin activity (% of initial) at each sampling point.Results: The effect of choline on VK₃ retention was significant in vitamin premixes (p<0.05). The negative effect of storage time was significant for the retentions of VD₃, VK₃, VB₁, VB₂, VB₅, and VB₆ in vitamin premix (p<0.05). For VTM premixes, negative effect of storage time was significant (p<0.05) for the losses of vitamin in VTM premixes. Choline and high concentrations of Cu and Zn significantly increased VA, VK₃, VB₁, and VB₂ loss during storage (p<0.05). The supplementation of high concentrations of Cu and Zn significantly decreased the concentrations of VD₃ and VB₆ (p<0.05) in VTM premixes at extended storage time.Conclusion: The maximum vitamin stability was detected in vitamin and VTM premixes containing no choline or excess Cu and Zn. The results indicated that extended storage time increased degradation of vitamin in vitamin or VTM premixes. These results may provide useful information for vitamin and VTM premixes to improve the knowledge of vitamin in terms of its stability.

A Pleiotropic Phenotype is Associated with Altered Endogenous Hormone Balance in the Developmentally Stunted Mutant (dsm1)

Hui-Fang Zhao,Kai Qiu,Guo-Dong Ren,Yong Zhu,Ben-Ke Kuai 한국식물학회 2010 Journal of Plant Biology Vol.53 No.1

A developmentally stunted mutant (dsm1) of Arabidopsis, isolated from an EMS mutant screen, had a pleiotropic phenotype, including repressed germination,retarded growth, delayed flowering, and impaired fertility. Additionally, dsm1 had a lifespan of approximately 160 days, which was more than twice the lifespan of the wild type (Col-0). Fine morphological and anatomical characters, such as the shoot apical meristem, root apical meristem, seed shape, and seed surface, were obviously altered in dsm1. We found that both abscisic acid and zeatin riboside levels were significantly greater in dsm1 than in Col-0 at all stages of development, while the levels of indole-3-acetic acid and gibberellins varied by age. The expressions of some abscisic acid-related genes were higher in dsm1 than in Col-0. These data indicate that DSM1 may play a general role in plant growth and development.

Two New Species in the Family Cunninghamellaceae from China

( Heng Zhao ),( Jing Zhu ),( Tong-kai Zong ),( Xiao-ling Liu ),( Li-ying Ren ),( Qing Lin ),( Min Qiao ),( Yong Nie ),( Zhi-dong Zhang ),( Xiao-yong Liu ) 한국균학회 2021 Mycobiology Vol.49 No.2

The species within the family Cunninghamellaceae are widely distributed and produce important metabolites. Morphological studies along with a molecular phylogeny based on the internal transcribed spacer (ITS) and large subunit (LSU) of ribosomal DNA revealed two new species in this family from soils in China, that is, Absidia ovalispora sp. nov. and Cunninghamella globospora sp. nov. The former is phylogenetically closely related to Absidia koreana, but morphologically differs in sporangiospores, sporangia, sporangiophores, columellae, collars, and rhizoids. The latter is phylogenetically closely related to Cunninghamella intermedia, but morphologically differs in sporangiola and colonies. They were described and illustrated.

CONTINUOUS DEPENDENCE PROPERTIES ON SOLUTIONS OF BACKWARD STOCHASTIC DIFFERENTIAL EQUATION

Fan, Sheng-Jun,Wu, Zhu-Wu,Zhu, Kai-Yong 한국전산응용수학회 2007 Journal of applied mathematics & informatics Vol.24 No.1

The existence theorem and continuous dependence property in $"L^2"$ sense for solutions of backward stochastic differential equation (shortly BSDE) with Lipschitz coefficients were respectively established by Pardoux-Peng and Peng in [1,2], Mao and Cao generalized the Pardoux-Peng's existence and uniqueness theorem to BSDE with non-Lipschitz coefficients in [3,4]. The present paper generalizes the Peng's continuous dependence property in $"L^2"$ sense to BSDE with Mao and Cao's conditions. Furthermore, this paper investigates the continuous dependence property in "almost surely" sense for BSDE with Mao and Cao's conditions, based on the comparison with the classical mathematical expectation.

The Effect of Transformation on the Virulence of Streptococcus pneumoniae

Zhang Xue-Mei,Yin Yi-Bing,Zhu Dan,Chen Bao-De,Luo Jin-Yong,Deng Vi-Ping,Liu Ming-Fang,Chen Shu-Hui,Meng Jiang-Ping,Lan Kai,Huang Yuan-Shuai,Kang Ge-Fei The Microbiological Society of Korea 2005 The journal of microbiology Vol.43 No.4

Although pneumococcus is one of the most frequently encountered opportunistic pathogen in the world, the mechanisms responsible for its infectiveness have not yet been fully understood. In this paper, we have attempted to characterize the effects of pneumococcal transformation on the pathogenesis of the organism. We constructed three transformation-deficient pneumococcal strains, which were designated as Nos. 1d, 2d, and 22d. The construction of these altered strains was achieved via the insertion of the inactivated gene, comE, to strains 1, 2 and 22. We then conducted a comparison between the virulence of the transformation-deficient strains and that of the wild-type strains, via an evaluation of the ability of each strain to adhere to endothelial cells, and also assessed psaA mRNA expression, and the survival of hosts after bacterial challenge. Compared to what was observed with the wild-type strains, our results indicated that the ability of all of the transformation-deficient strains to adhere to the ECV304 cells had been significantly reduced (p < 0.05), the expression of psaA mRNA was reduced significantly (p < 0.05) in strains 2d and 22d, and the median survival time of mice infected with strains Id and 2d was increased significantly after intraperitoneal bacterial challenge (p < 0.05). The results of our study also clearly indicated that transformation exerts significant effects on the virulence characteristics of S. pneumoniae, although the degree to which this effect is noted appears to depend primarily on the genetic background of the bacteria.

The Effect of Transformation on the Virulence of Streptococcus pneumoniae

Xue-Mei Zhang,Yi-Bing Yin,Dan Zhu,Bao-De Chen,Jin-Yong Luo,Yi-Ping Deng,Ming-Fang Liu,Shu-Hui Chen,Jiang-Ping Meng,Kai Lan,Yuan-Shuai Huang,Ge-Fei Kang 한국미생물학회 2005 The journal of microbiology Vol.43 No.4

Although pneumococcus is one of the most frequently encountered opportunistic pathogen in the world, the mechanisms responsible for its infectiveness have not yet been fully understood. In this paper, we have attempted to characterize the effects of pneumococcal transformation on the pathogenesis of the organism. We constructed three transformation-deficient pneumococcal strains, which were designated as Nos. 1d, 2d, and 22d. The construction of these altered strains was achieved via the insertion of the inactivated gene, comE, to strains 1, 2 and 22. We then conducted a comparison between the virulence of the transformation-deficient strains and that of the wild-type strains, via an evaluation of the ability of each strain to adhere to endothelial cells, and also assessed psaA mRNA expression, and the survival of hosts after bacterial challenge. Compared to what was observed with the wild-type strains, our results indicated that the ability of all of the transformation-deficient strains to adhere to the ECV304 cells had been significantly reduced (p < 0.05), the expression of psaA mRNA was reduced significantly (p < 0.05) in strains 2d and 22d, and the median survival time of mice infected with strains 1d and 2d was increased significantly after intraperitoneal bacterial challenge (p < 0.05). The results of our study also clearly indicated that transformation exerts significant effects on the virulence characteristics of S. pneumoniae, although the degree to which this effect is noted appears to depend primarily on the genetic background of the bacteria.