후두 및 하인두 암종에서 p53단백 발현과 Espstein-Barr Virus 검출

최영환,도남용,나한조,이도용,노용훈,김완수,최종선 조선대학교 2001 The Medical Journal of Chosun University Vol.26 No.2

Background and Objectives : When p53 cancer suppressor gene, occurs gene deletion or point mutation, malignancy develops by loss of p53 function with abnormal p53 protein. The Epstein-Barr virus (EBV) is the causative agent of certain type of lymphoma and undifferentiated nasopharyngeal carcinoma. However, the role of EBV as a causative factor in other head &eck tumors is not fully elucidated except nasopharyngeal carcinoma. This study, the author examined that p53 expression and detection rate of EBV correlate to development of laryngeal and hypopharyngeal squamous cell carcinoma(SCC) and play the possible role of prognostic indicators. Materials and Methods : Formalin-fixed, paraffin-embedded tissue specimen from 32 cases of larynx and hypopharyngeal squamous cell carcinomas were studied by immunohistochemical staining for p53 and EBV. The results of analysis were compared with clinicopathological parameters. Results : p53 expression was 56.3% (18 cases) and the detection rate of EBV was 43.6% (14 cases) of 32 cases in laryngeal and hypopharyngeal squamous cell carcinomas. p53 expression was correlate with histologic grade (p<05) only. Detection of EBV was not correlate with clinicopathological parmeters. Conclusion : These results suggest p53 expression and the detection of EBV may be related with development of laryngeal and hypopharyngeal squamous cell carcinoma. And, the expression of p53 protein can be used as a prognosticator in laryngeal and hypopharyngeal squamous cell carcinoma under certain limitation.

운동과 사우나시의 발한성분 분석

김영수,김병로 창원대학교 체육과학연구소 1998 경남 체육연구 Vol.3 No.2

본 연구는 운동과 사우나시의 발한성분 중 전해질과 금속이온 농도의 차이를 비교. 분석하여 운동과 사우나의 생리적 효과를 규명하고자 하는 데 그 목적이 있었다. 이를 위하여 C 대학교에 재학중인 운동선수 10명과 비운동선수 10명을 대상으로 트레드밀 운동과 건식 사우나를 실시하여 전해질과 금속 농도를 분석한 결과 다음과 같은 결론을 얻었다. 1. 전해질 중 Na^+, CL^-, K^+, CA²+, Mg^2+, So₄²-의 농도는 운동시가 사우나시 보다 높았다. 2. 전해질 중 Na^+, CL^-, K^+, CA²+, Mg^2+, So₄²-의 농도는 운동선수 집단이 비운동선수 집단 보다 낮았고, CA^²+의 농도는 운동선수와 비운동선수 집단 간에 차이가 없었다. 3. 금속 중 Cu, Mn의 농도는 운동시가 사우나시 보다 높았고, Fe의 농도는 운동시와 사우나시 간에 차이가 없었다. 4. 금속 중 Fe, Mn의 농도는 운동선수 집단이 비운동선수 집단 보다 낮았고, Cu의 농도는 운동선수와 비운동선수 집단 간에 차이가 없었다. 이상의 결론을 종합해 보면, 전반적으로 운동선수 집단이 비운동선수 집단 보다 전해질 및 금속 이온의 농도가 낮게 나타났고 운동시가 사우나시 보다 전해질 및 금속이온의 농도가 높게 나타난 것으로 보아, 평상시 규칙적인 운동이 체온조절과 대사작용 및 발한성분에 긍정적인 영향을 미치는 것으로 생각되며, 운동시가 사우나시에 비해 전해질 및 금속이온이 많이 배출되었다는 점에서 이에 대한 계속적인 반복연구가 필요할 것으로 생각된다.

Pseudomonas sp. LBC505의 섬유소 분해효소 특성

정영철,강신권,김희숙,노종수 진주여자전문대학 1995 論文集 Vol.18 No.-

The Pseudomonas sp. LBC505 isolated from soil, produced cellulolytic enzymes such as carboxymethyl cellulase, avicelase, β-1,4-glucosidase, and xylanase in medium. The cellulase complex exhibited maximal activity at pH6.0-pH6.5, and 50-55℃, and was stable in the range of pH5.0 to 9.0 and up to 65℃ for 30min. The cellulase complex and xylanase activities were completely inhibited in the presense of 1mM HgCl₂ and 1mM AgCl₂. The enzyme activity was stimulated in the presense of 100mM mercaptoethanol, suggesting that sulfhydryl groups play an important role in the catalytic action. The crude cellulolytic enzyme hydrolyze CMC to produced glucose, maltose, maltotriose, maltotetraose, maltopentose and maltohexose by synergistic action of cellulase complex.

단백질 분해효소 생성 호염성세균의 분리 및 효소 생성

정영철,김양우,노종수,강인수 진주여자전문대학 1996 論文集 Vol.19 No.-

For the accelerating fermentation of high salt anchovy using halophilic proteolytic microorganisms, various halotolerant or halophilic protease-producing strains were isolated from 13 types of high salt fermented fish pastes. Among 13 halotolerant and 4 halophilic isolates which produced proteases on PS agar medium containing a different NaCl concentration from 0% to 20%, one halotolerant and protease-producing strain, CK-14 was identified as a member of Bacillus sp., and another halophilic and protease-producing strain , LF-7, a member of Vibrio sp. through fatty acid composition analysis. The NaCl range for growth and protease production of Bacillus sp. CK-14 and Vibrio sp. LF-7 in PS broth was extended from 0% to 12.5%, and from 5% to 20%, with an optimal concentration of 5% and 7.5%, respectively. The optimal temperature and pH for cell growth and protease production of Bacillus sp. CK-14 and Vibrio sp. LF-7 were 30℃ and 35℃, pH range of 8.0 to 8.5 and 6.5 to 9.5, respectively. The maximal protease activity of Bacillus sp. CK-14 was 3200 unit after 6% incubation, and that of Vibrio sp. LF-7 was 3980 unit after 120hr incubation under optimal culture conditions.

PH 4.3에서 재광화 용액의 포화도에 따른 인공 탈회된 법랑질의 동력학적 변화

이지숙,노병덕,신수정,이윤,공형규,이찬영 대한치과보존학회 2009 Restorative Dentistry & Endodontics Vol.34 No.1

법랑질의 재광화에 영향을 주는 요인으로는 PH, 불소 농도, 포화도 등이 있다. 본 연구에서는 유산 완충 탈회용액을 이용하여 법랑질 시편을 인공 탈회시킨 후, pH 4.3에서 포화도를 0.22, 0.30, 0.35로 달리한 세가지 재광화 용액에 10일간 처리하여 나타나는 변화를 편광현미경으로 관찰하여 전체 탈회 깊이와 건전 표층 폭의 변화를 조사하였다. 또한 Image program (Scion Image analyzer)을 이용하여 병소 부위의 평균 mineral density를 측정하여 탈회와 재광화 후 무기질 변화량을 통해 인공 탈회된 법랑질의 동력학적 변화를 관찰하였다. 1 재광화 후 모든 군에서 건선 표층의 폭이 증가하였는데, 포화도가 증가할수록 건전 표층 폭이 증가하였다 (P< .05) 2 재광화 후 mineral density 변화를 관찰한 결과 포화도가 낮은 군에서는 이온의 침착이 병소 전반적으로 일어났으나, 포화도가 높은 군에서는 건전 표층 부위와 표층하 병소의 하층부에서 이온의 침착으로 mineral density 가 증가하였고 표층하 병소의 상층부에서는 탈회가 진행되어 mineral density가 감소하였다. 3 재광화 후 모든 군에서 무기질량이 증가하였고 전체 탈회 깊이도 증가하였으나 각 군간에 통계적 유의차는 없었다. 본 실험에서 인공 탈회된 시편을 pH 4.3인 재광화 용액에 처리시 포화도가 높을수록 건전 표층에서 더 많은 재광화 현상이 일어났고 표층하 병소에서는 재광화 현상이 적게 일어났으며 재광화 후 모든 군에서 전체 탈회 깊이는 증가하였다. The purpose of this study is to observe and compare the dynamic change of artificially demineralized enamel by remineralization solutions of different degrees of saturation at pH 4.3 In this study, 30 enamel specimens were demineralized artificially by lactic acid buffered solution. Each of 10 specimens was immersed in pH 4.3 remineralization solution of three different degrees of saturation (0.22, 0.30, 0.35) for 10 days. After demineralization and remineralization, images were taken by a polarizing microscope (× 100). The density of lesion were determined from images taken after demineralization and remineralization. During remineralization process, mineral deposition and mineral loss occurred at the same time. After remineralization total mineral amount and width of surfacc lesion increased in all groups. The higher degree of saturation was, the more mineral deposition occurred in surface lesion and the amount of mineral deposition was not much in subsurfacc lesion. Total demineralized depth increased in all groups.

호알칼리성 Bacillus sp.F204와 Bacillus sp.K17의 원형질체 융합

성낙계,노종수,박석규,정영철 순천대학교 기초과학연구소 1990 基礎科學硏究誌 Vol.1 No.-

To develop cellulase and xylanase-producing strain by protoplast fusion, alkalophilic Bacillus sp. F204 and K17 were treated with NTG(N-methyl-N'-nitro-N-nitrosoguanidine) and isolated antibiotics resistant strains of S20 (Km', Cm') and G70(Str'). The frequency of protoplast formation was about 95% when cells of mid-log phase were treated with 200㎍/㎖ lysozyme at 37℃ for 30-45 minutes. Under addition of 0.4-0.5M sodium succinate, 0.5% casamino acid, 1.5% polyvinylpyrrolidone, 25mM MgCl_2 and 50mM CaCl_2 to the regeneration medium, the regeneration frequency of Bacillus sp. F204 and K17 was 24.9% and 26.2%, respectively. the fusion frequency was 6.6×10^-6 in the presence of 30% polyethylene glycol 6000 containing 50mM Ca^++ at 45℃ for 5 minutes. Cellulase complex and xylanase activities of fusant were compared with parental strains.

온도자극이 충전된 치질에 미치는 영향

김재곤,노용관,이영수,양정숙,백병주 大韓小兒齒科學會 1999 大韓小兒齒科學會誌 Vol.26 No.2

본 연구에서는 수복에 따른 치질 내부 구조물의 열적변화를 평가하기 위하여 와동이 형성된 인공 치아모형에 아말감 충전, 아말감과 스테인레스 스틸관 수복, 금인레이와 금관수복, ZOE 이장 및 아말감 충전등으로 수복한 후 온도변화를 관찰하였다. 각 실험군에 4℃와 60℃로 2초와 4초 동안 자극을 가한 후 치아내부의 온도분포를 2차원적으로 분석하였고, 치수 중앙부에서 외측으로 층을 형성하여 3차원적인 해석을 시행한 결과, 다음과 같은 결론을 얻었다. 1. 4℃의 자극이 2초간 가해진 아말감 충전 경우, 아말감 충전과 스테인레스 스틸관을 장착한 경우에서는 1℃ 내외의 차이를 보였으며, 치수와 상아질 경계면에서 3초 경과시에 29℃까지 급격한 온도 하강을 보였으며 9초 후에 25℃에 도달하였다. 또한 금으로 수복한 경우에서는 3초 후 25℃까지 하강하여 그 온도를 유지하였으며, ZOE 이장 경우에서는 최저온도가 4℃ 이상 높게 나타났다. 2. 4℃ 자극을 4초간 가한 경우에서는 9초 후에서 2초 자극시보다 2-3℃ 낮았으며, 금 수복 경우에는 5초 후에 21℃의 최저온도를 보인 후 점차 회복되었다. 3. 아말감 충전 및 스테인레스 스틸관을 같이 장착한 경우에 60℃, 2초간 자극에는 3초 후 40℃의 온도로서 상승하였으나 9초 후에 30℃로 하강 하였으며, 금 수복에서는 2초 후 41℃까지 온도가 상승하였고, 9초 후 28℃를 보여 13℃의 차이가 있었다. ZOE 이장 경우에는 온도차이가 5℃로서 안정된 양상을 보였다. 4. 60℃, 4초 자극에 대한 아말감 충전 및 스테인레스 스틸관을 같이 장착한 경우에서 5초 후 42℃, 9초후 35℃를 보였으나 금 수복 경우에서는 3초 후 49℃, 9초 후 31℃의 온도변화를 나타내었다. 5. 3차원 분석에서 치수 중앙부에서 멀어질수록 온도변화가 심하였다. The dental structure substituted by restorative materials may produce discomfort resulting from hot or cold stimuli. To investigate the effects of this stimuli on the human teeth, thermal analysis was carried out by calculation of general heat conduction equation in a modeled tooth using numerical method. The method has been applied to axisymmetric and two-dimensional model, analyzing the effects of constant temperature 4℃ and 60℃. That thermal shock was provided for 2 seconds and 4 seconds, respectively and recovered to normal condition of 20℃ until 10 seconds. The thermal behavior of tooth covered with a crown of gold or stainless steel was compared with that of tooth without crown. At the same time, the effects of restorative materials(amalgam, gold and zinc oxide-eugenol(ZOE)) on the temperature of PDJ(pulpo-dentinal junction) has been studied. The geometry used for thermal analysis so far has been limited to two-dimensional as well as axisymmetric tooth models. But the general restorative tooth forms a cross shaped cavity which is no longer two-dimensional and axisymmetric. Therefore, in this study, the three-dimensional model was developed to investigate the effect of shape and size of cavity. This three-dimensional model might be used for further research to investigate the effects of restorative materials and cavity design on the thermal behavior of the real shaped tooth. The results were as follows: 1. When cold temperature of 4℃ was applied to the surface of the restored teeth with amalgam for 2 seconds and recovered to ambient temperature of 20℃, the PDJ temperature decreased rapidly to 29℃ until 3 seconds and reached to 25℃ after 9 seconds. This temperature decreased rather slowly with stainless steel crown, but kept similar temperature within 1℃ differences. Using the gold as a restorative material, the PDJ temperature decreased very fast due to the high thermal conductivity and reached near to 25℃ but the temperature after 9 seconds was similar to that in the teeth without crown. The effects of coldness could be attenuated with the ZOE situated under the cavity. The low thermal conductivity caused a delay in temperature decrease and keeps 4℃ higher than the temperature of other conditions after 9 seconds. 2. The elapse time of cold stimuli was increased also until 4 seconds and recovered to 20℃ after 4 seconds to 9 seconds. The temperature after 9 seconds was about 2-3℃ lower than the temperature of 2 seconds stimuli, but in case of gold restoration, the high thermal conductivity of gold caused the minimum temperature of 21℃ after 5 seconds and got warm to 23℃ after 9 seconds. 3. The effects of hot stimuli was also investigated with the temperature of 60℃. For 2 seconds stimuli, the temperature increased to 40℃ from the initial temperature of 35℃ after 3 seconds of stimuli and decreased to 30℃ after 9 seconds in the teeth without crown. This temperature was sensitive to surface temperature in the teeth with gold restoration. It increased rapidly to 41℃ from the initial temperature of 35℃ after 2 seconds and decreased to 28℃ after 9 seconds, which showed 13℃ temperature variations for 9 seconds upon the surface temperature. This temperature variations were only in the range of 5℃ by using ZOE in the bottom of cavity and showed maximum temperature of 37℃ after 3 seconds of stimuli. 4. In case of 4 seconds hot stimuli, the temperature increased 42℃ after 5 seconds and decreased 35℃ after 9 seconds in the teeth without crown. But in gold restoration, showed 49℃ after 3 seconds and 31℃ after 9 seconds, temperature variation was up to 18℃. Temperature variation of ZOE base case was only 5℃. 5. In three-dimensional analysis, we could find higher and lower thermal distribution pattern at the outer layer.

캐릭터 연동 온라인 3D 게임을 위한 실시간 모션캡쳐 시스템 개발

이준호,유영기,노병욱,이수진 선문대학교 ·중소기업기술지원연구소 2002 선문공대 연구/기술 논문집 Vol.7 No.1

최근들어 3차원 가상현실의 발전과 더불어 동작을 트랙킹하는 모션캡쳐는 엔터테인먼트, 의학, 스포츠, 교육, 산업등에 다양하게 널리 사용되어지고 있다. 특히 엔터테인먼트분야의 애니메이션과 게임등에서는 캐릭터의 생성에 모션캡쳐 기술을 사용하는데 기존에는 마우스, 조이스틱, 미디 슬라이더(Midi Sliders) 및 기타 장치들을 사용하여 캐릭터를 직접 연기시키는 방법을 사용하였으나 모션캡쳐에 의한 방법은 이전에 볼 수 있었던 그 어떤 방법보다도 자연스러운 움직임을 만들어낼 수 있다. 본 연구에서는 캐릭터를 연동한 온라인 3D 게임을 실현하기 위하여 광센서를 이용한 저가이면서도 빠른 샘플링 빈도를 갖는 모션캡쳐 시스템을 개발하고자 한다. Motion trackings are being used in part of the entertainment, medical, sports, education and industry with the development of 3D virtual-reality. In particular, the technology is used for animation and games as a part of entertainment. We can make very smooth motion that was never realized before, if we use the technology instead of using mouse, joystick, midi-sliders and so on for making some characters. In this research, we have developed a very low priced and fast sampling system using optic sensors for capturing the motion.

위험평가(Risk Assessment)에 의한 업종별 위험(Risk)의 구분

박동욱,박덕묵,정광수,윤충식,김태형,노영만,이경남,이송권,김현욱 한국산업위생학회 2003 한국산업보건학회지 Vol.13 No.2

A total of 514 workplaces in 17 industries were inbestigated through walk-through survey. These industries were all small companies with less than five workers and didn't have work environmental measurement data. These industries were ranked by risk that was evaluated by combination of toxicity and possibility of exposure to chemical hazardous agents produced at operations of industry. Risk Index(RI) was qualitatively combined by the combination between hazard and potential of exposure to chemical hazardous agents. Industries that were regarded as having the highest risk were wood and products of wood, chemicals and chemical products, basic metals, other vehicles, trailers and semi-trailers, and fumiture manufacturing. These industries were found to have operations with higher risk than other industries. This study found that more attentions should be paid to these industries with that more attentions should be paid to these industries with high risk.

Reconstruction of the Electron Density Profile in O-mode Ultrashort Pulse Reflectometry using a Two-dimensional Finite Difference Time Domain

Roh, Young-Su The Korean Institute of IIIuminating and Electrica 2013 조명·전기설비학회논문지 Vol.27 No.7

The two-dimensional finite difference time domain algorithm is used to numerically reconstruct the electron density profile in O-mode ultrashort pulse reflectometry. A Gaussian pulse is employed as the source of a probing electromagnetic wave. The Gaussian pulse duration is chosen in such a manner as to have its frequency spectrum cover the whole range of the plasma frequency. By using a number of numerical band-pass filters, it is possible to compute the time delays of the frequency components of the reflected signal from the plasma. The electron density profile is reconstructed by substituting the time delays into the Abel integral equation. As a result of simulation, the reconstructed electron density profile agrees well with the assumed profile.