Effects of Interleukin-1β, Tumor Necrosis Factor-α and Interferon-γ on the Nitric Oxide Production and Osteoclast Generation in the Culture of Mouse Bone Marrow Cells

Kwon, Young-Man,Kim, Se-Won,Ko, Seon-Yle The Korean Academy of Oral Biology 2006 International Journal of Oral Biology Vol.31 No.2

Nitric oxide(NO) is a labile, uncharged, reactive radical that functions as a sensitive mediator of intercellular communication in diverse tissues. It has been reported that NO is produced by osteoblast and these results may suggest that NO is integrally involved in the regulation of osteoclast formation and osteoclast resorption activity by osteoblastic cells. We examined the effect of cytokines on NO release by mouse bone marrow cell. We also examined the effects of cytokokines and sodium nitroprusside(SNP) on the formation of osteoclast-like cell from mouse bone marrow cells in culture. Cytokines stimulated NO production of mouse bone marrow cells, and N-nitro-L-arginine methyl ester, a specific inhibitor of NO synthase, suppressed the cytokine-induced NO production. SNP showed dual action in the generation of osteoclasts. The addition of 30μM SNP inhibited the formation of tartrate resistant acid phosphatase(TRAP)(+) multinucleated cell, whereas lower concentration(3μM) of SNP enhanced it. Althogh the precise action of NO remains to be elucidated in detail, the action of NO in osteoclast generation in our studies seems to be associated, at least in part, with bone matabolism and bone pathophysiology.

1RM 적용에 따른 강도별 웨이트트레이닝 운동이 혈역학 반응과 심근 부담율에 미치는 영향

권만근(Kwon, Man-Geun),김영주(Kim, Young-Joo) 한국체육과학회 2017 한국체육과학회지 Vol.26 No.1

The purpose of this study is to investigate the effect of weight exercise on physiological response according to weight by comparing the hemodynamic response and rate pressure product (RPP) according to weight exercise of each area at 60% and 80% of 1RM. Treadmill and 1RM tests were conducted in healthy 20’s men. Also, the ratio of bench press (BP), deadlift (DL), military press (MP), squat (SQ), and behind press (BNP) at 6 0 and 80% of 1RM according to HR, SBP, RPP, MRPP was measured. At 60% o f 1RM in HR, DL, MP, SQ, and BNP were significantly higher than BP (p<.001, p<.01, p<.001), and SQ was significantly higher than MP(p<.05). In SBP, DL was significantly higher than BP and MP(p<.01, p<.05), and SQ was significantly higher than BP, MP, and BNP(p<.001, p<.001, p<.01). In RPP, BP was significantly lower than DL, SQ, and BNP(p<.001, p<.001, p<.05), and DL was significantly higher than MP(p<.001). SQ was significantly higher than BP, MP, and BNP(p<.001, p<.001, p<.05). In the ratio to MRPP reach, DL was significantly higher than BP(p<.01), and SQ was significantly higher than BP, MP, and BNP(p<.001, p<.001, p<.01). At 80% of 1RM in HR, B P was significantly lower than D L, M P, SQ, a nd BNP(p<.001, p<.001, p<.001, p<.01), and SQ was significantly higher than BNP(p<.05). In SBP, DL and SQ was significantly higher than BP, MP, BNP(p<.001). In RPP, DL was significantly higher than BP, MP, and BNP(p<.001, p<.001, p<.01), and SQ was sigficantly higher than BP, MP, and BNP(p<.001). At MRPP reach ratio, DL was significantly higher than BP, MP, and BNP(p<.001, p<.01, p<.01), and SQ was significantly higher than BP, MP, and BNP(p<.001). In conclusion, when applying 60% and 80% of 1RM of each weight exercise, RPP was clealy increased according to high blood pressure response in DL and SQ, and there was lower heart rate compared with other exercise type in BP. The safe exercise prescription should be necessary when applying weight exercise for patients who need cautions of weight exercise.

장기간 항갑상선제 투여 후 임의 중단 뒤 재복용시 발생한 무과립구증 1 예

김승만;김태영;박수영;배영환;서영경 정은주;김태균;권민정;박정현;이순희 인제대학교 2011 仁濟醫學 Vol.32 No.-

Agranulocytosis, one of the major side effects of antithyroid a gents, probably occurs in less than 0.25% of cases treated with antithyroid agents. It usually occurs within the first several weeks or months after the initiation of therapy, when drug dosage is higher. Most reported cases of agranulocytosis have occurred during the first 3 months of therapy. We describe here a rare case that did not experience the side effect for 6 years of antithyroid agent treatment but presented with agranulocytosis in one month after re-exposure. It suggests that the major side effect such as agranulocytosis can occur in patients who had undergone prior uneventful antithyroid drug therapy.

두통을 주소증으로 하여 입원한 환자 120예에 대한 임상적 고찰

권경만 ( Kyoung Man Kwon ),김광록 ( Koang Lok Kim ),신윤리 ( Yoon Ri Shin ),박선영 ( Sun Young Park ),박동일 ( Dong Il Park ) 경락경혈학회 2012 Korean Journal of Acupuncture Vol.29 No.4

Objectives: The Purpose of this study is to investigate clinical characteristics with 120 patients who have sufferred from headache and were treated. Methods: We classified 120 patients into several groups by International Headache Society(IHS) classification and evaluated the effects of oriental medical therapy on headache. Results: 1. According to the statistics, women patients were more than men. And 50s has highest number of headache patients. 2. In classification by IHS, tension type headache were highest. 3. 24.2% of patients had entered Department of Internal Medicine, Colleage of Oriental Medicine Dongeui Univ for treatment within six month of onset. 4. According to the statistics, headache of whole part has highest number of headache patients. 5. The most number of associated symptoms were nausea, vomiting, dyspepsia etc. 6. The most number of headache patient`s past history were hypertension. 7. In classification by oriental medical differentiation of symptom and signs, the rate of blood deficiency were highest. 8. 64.2% of patients said they were satisfied with treatment, reporting half the frequency of headache or better from before treatment. Conclusions: In conclusion, the present results suggest that oriental medical therapy has effects on headache.

중국 길림성 조선족의 민족 정체감에 관한 연구

이호영,이동근,신승철,김만권 大韓神經精神醫學會 1992 신경정신의학 Vol.31 No.2

The present study examined the structure and patterns of ethnic identity in a sample of Korean-Chinese parents and their adolescents residing in the Jilin Province in China. The ethnic identity of these participants(total :148 parents:100 adolesents:48) includes two major components : Korean identity and Chinese identity. Each of these components was measured in three different modalities : behavioral, affective and cognitive. The results are summarized as follow ; 1) Generational comparisons between Korean identity scores of parents and adolescents indicated similarities in the modalities of cognitive and behavioral identities. Both groups shared a Korean orientation manifested in their high level of Korean behavior and cognition. However, both groups also shared a relatively high level of Chinese identity. In summary, dual identity appeared to be preserved over time. The parents scored higher than the adolescents in their affective modality of Korean identity. Chinese identity was scored higher in the adolescents than korean identity in their affective modality. The generational comparisons indicated that the adolescents were acculturated in their affect than their parents. 2) Comparison of men and women in the strength of identity' component showed higher cognitive scores among the men. in Korean identity, compared with Chinese identity. Men were more identified with "Chinese" and "Korean" in the affective component of their identity.

法人稅平準化 測定에 관한 硏究

權義晩,韓永熙,鄭柔錫 군산대학교 산업개발연구소 1997 産業開發硏究 Vol.16 No.-

This paper investigates the problems in the measurement regarding corporate income tax smoothing. Past studies generalyy based conclusions on the degree or frequency of apparent tax smoothing behavior identified among sample firms. This investigated two problems in the past studies. The first problem is that much of what appears as tax smoothing may occur "naturally" i.e., without any conscious intent by management to regulate fluctuations in its exemption codes. The second problem lies in the measurement ration of tax smoothing, which may overestimate the fluctuations is corporate income tax. In sum, this study proposed a general methods which taked the possibilities naturally tax smoothing by management and provided the method exactly to measure the smoothness.

보너스보상계획과 이익조절에 관한 연구

권의만,한영희 군산대학교 산업개발연구소 1998 産業開發硏究 Vol.17 No.-

Using confidential accounting data of executive-specific short-term bonus plans, we presents the extent to which executives manipulate earnings to maximize the present value of bonus plan payments. As such, this paper extends the works of other, Healy(1985). Like Healy, we find essential model evidence consistent with the hypothesis that managesr manipulate earnings downwards when their bonuses are at their maximum. Unlike Healy, we find essential model no evidence that managers manipulate earnings downwards when earnings are below the minimum ecessary to receive any bonus.

해마 theta리듬과 pyramidal neuron의 세포내 특성과의 상관관계

권오흥,조진화,남순현,김현정,최병주,김영진,이만기 大韓小兒齒科學會 1998 大韓小兒齒科學會誌 Vol.25 No.4

Eledctrophysiological phenomena of pyramidal cells in the CAI area of the dorsal hippocampus were recorded from and filled with neurobiotin in anesthetized rats. The electropharmacological properties of membrane as well as the cellular-synaptic generation of rhythmic slow activity (theta)were examined. The intracellular response characteristics of these pyramidal cells were distinctly different from responses of inteneurons. Pyramidal cells had a high resting membrane potential, a low input resistance, and a large amplitude action potential. A afterhyperpolarization was followed a single action potential. Most of pyramidal cells did not display a spontaneous firing. Pyramidal cells displayed weak inward rectification and anodal break excitation. The slope of the frequency-current relation was 53.4 Hz/nA for the first interspike interval and 15.9 Hz/nA for the last intervals, suggesting the presence of spike frequency adaptation. Neurobiotin-filled neurons showed pyramidal morphology. Cells were generally bipolar dendritc processes ramifying in stratum lacunosum-moleculare, radiatum, and oriens. Commissural stimulation discharged pyramidal cells, followed by excitatory and inhibitory postsynaptic potentials (EPSPs and IPSPs). The frequency of theat-related membrane potential oscillation was voltage-independent in pyramidal neurons. At strong depolarization levels (less than 30 mV) pyramidal cells emitted sodium spike oscillation, phase-locked to theta. The observations provide direct evidence that theta-related rhythmic hyperpolarization of principal cells is brought by the rhythmically discharging interneurons. Furthermore, the findings in which interneurons were also paced by rhythmic inhibitory postsynaptic potentials during theta suggest that they were periodically hyperpolarized by their GABAergic septal afferents.

연 X-선 현미경을 이용한 금 나노입자 세포영상

권영만,김한경,김경우,김선희,윤홍화,천권수,강성훈,박성훈,정선관,윤권하 韓國電子顯微鏡學會 2008 Applied microscopy Vol.38 No.3

연 x-선 현미경은 ‘물의 창’ 영역(2.3~4.4 nm)의 파장을 이용하여, 수십 nm의 분해능으로 세포를 파괴하지 않고 살아있는 상태에서 세포의 내부구조를 관찰할 수 있어 가시광선현미경과 전자현미경을 단점을 보완하는 특징을 갖는 세포 생물학 연구에 적합한 현미경이다. 그러나 기존 연 x-선 현미경은 광원으로 방사선 가속기를 이용하기 때문에 사용이 제한적이었다. 이에, 본 연구에서는 2.88 nm의 연 x-선을 광원으로 사용하는 소형 연 x-선 현미경을 이용하여, 내포작용에 의해 금 나노입자를 포획한 HT1080과 MDA-MB 231 세포의 영상을 약 60 nm 분해능으로 획득하였다. 금 나노입자의 세포에 대한 독성을 제거하기 위하여 폴리에틸렌 글리콜을 캡핑하였고, 2.88 nm 파장의 연 x-선에 대하여 충분한 조영효과로 인하여 세포영상에서 뚜렷한 대조도를 나타내었다. 내포작용에 의해 액포에 포함되어 있는 다양한 크기의 금 나노입자 군집을 확인하였으며, 세포내부의 액포의 분포상태도 관찰할 수 있었다. 따라서 고분해능을 가진 소형 연 x-선 현미경을 이용하여 금 나노입자를 세포내의 미세기관이나 특정 단백질에 표지하면 연 x-선에 대한 조영효과의 증가에 의하여 더욱 유용한 정보를 획득할 수 있을 것으로 생각한다. A compact soft x-ray microscope operated in the ‘water window’ wavelength region (2.3~4.4 nm) was used for observing cells with nano-scale spatial resolution. To obtain cellular imaging captured with colloidal gold nanoparticles using a compact soft x-ray microscope. The colloidal gold nanoparticles showed higher contrast and lower transmission more than 7 times than that of cellular protein on the soft x-ray wavelength region. The structure and thickness of the cell membrane of the Coscinodiscus oculoides (diatome) and red blood cells were seen clearly. The gold nanoparticles within the HT1080 and MDA-MB 231 cells were seen clearly on the soft x-ray microscopy. The gold nanoparticles were aggregated within vesicles by endocytosis.

국내에서 생장한 Kenaf의 해부학적 특성

권영만,황원중,권성민,조준형,이명구,김남훈 江原大學校 森林科學硏究所 2005 Journal of Forest Science Vol.21 No.-

국내에서 재배한 kenaf의 해부학적 특성을 광학현미경법으로 조사하였다. Kenaf의 수피부에는 인피섬유, 사부방사조직, 피층유세포 등이 존해하였고, 목질부는 도관, 목섬유, 방사조직 등으로 구성되어 있었다. 도관은 고립관공과 2~3개가 방사방향으로 복합한 복합관공이 많이 존재하였고, 방사조직은 평복, 직립, 방형세포가 모두 분포하고 있었다. 접선단면에서 방사조직은 단열 및 다열방사조직으로 구성되었다. 수피비의 인피섬유층은 생장기간과 함께 증가하였다. Anatomical properties of Kenaf cultivated in Korea was investigated using light microscopy. Bast fiber, phloem ray and cortex parencyma cell were observed in bast, and vessel, wood fiber and rat in core. A lot of solitary and multiple radial pores in core existed. The cell type of ray parenchyma in radial section was procumbent, upright and square sell. Uniseriate and multiseriate rays existed in tangential section. The layer of bast fiber in bast increased with in creasing the growth period.