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      • KCI등재

        State Indicator of Water Quality for Surface Water and Groundwater in Agriculture

        Min-Kyeong Kim,Goo-Bok Jung,An-Sung Ro,Seung-Chul Choi,Won-Il Choi,Eun-Jeong Kim,Jong-Eun Lee,Yeo-Uk Yun,Kab-Cheol Kim,Do-Young Ko,Byeong-Ho Kim,Hyeon-Ji Kim,Sang-Jo Park,Seong-Tae Lee,Jae-Young Heo,S 한국토양비료학회 2015 한국토양비료학회지 Vol.48 No.5

        Indicators of environmental conditions qualitatively and quantitatively describe the state of the environment and natural resources, and the OECD (Organization for Economic Cooperation and Development) has initiated and suggested AEIs (Agri-Environmental Indicators) to assess trends over time of the effects of agriculture on the environment and the effectiveness and efficiency of agricultural and environmental policy measures since 1990’s. This study aims to develop the state indicators of water quality for agricultural water, surface and groundwater, to evaluate the environmental impacts of agricultural activities and policies by qualifying the environmental levels of a nation. Status indicators were calculated according to the agricultural water quality standards of OECD and Korea, and their trends were analyzed over time. Particularly, nitrate(NO₃<SUP>-</SUP>) status indicators of ground water in 2013 were significantly lower than the ones in 2000. Overall, the water quality indicators of surface water in 2013 were higher than the ones in 2012, except for pH and DO. The groundwater quality indicators in 2013 were lower than those of previous years. The optimal management indicators were calculated to assess agricultural surface water and groundwater quality. The findings of this study indicated that the state indicators could play a significant role to establish policies and procedures for managing and conserving water resources. This study also discussed water pollution caused by agricultural and industrial effluents.

      • 돼지 신장세포(PK15)의 이종이식 거부반응에 대한 재조합 IL-18의 영향

        최연실,김은미,김영관,박상연,심정현,윤도영,강형식,명평근 충남대학교 형질전환복제돼지연구센터 2004 논문집 Vol. No.8

        Various cytokines including IL-1β, IL-2, IL-4, IL-10, IL-12p40, IFN-r, and TNF-α have been recently reported to play important roles in both host and donor cell activations during xenotransplantation. Interleukin (IL)-18, a proinflammatory cytokine, can induce IL-4 and IL-13 production in T cells, NK cells, B cells, mast cells, and basophils. IL-18 has the capacity to stimulate innate immunity and both Th1-and Th2- mediated responses, but little is known to involve in the xenograft rejection. In order to investigate the role of IL-18 in xenograft rejection, we transplanted the pig kidney (PK15) cells to C57BL/6 mouse with or without intraperitoneal injection of mouse recombinatant IL-18, It was analyzed the population of T cell, B cell and NK cell in the mice transplanted with PK 15 cells and recombinant IL-18 by flow cytometry. We found that splenic CD3+ T cells were increased in mice injected PKl5 cells with recombinant IL-18. These results suggest that recombinant IL-18 is critical in the xenograft rejection by increasing T cell population of the recipients.

      • KCI등재

        재조합 균주 Bacillus subtilis LKS88에 의한 Streptomyces albus KSM-35 Amylase의 생산조건

        최원진,유도진,이재우,소명환,김영배 한국식품영양학회 1998 韓國食品營養學會誌 Vol.11 No.4

        Streptomyces albus KSM-35의 amylase의 유전자를 Bacillus subtilis LKS88에서 발현시킨 균주를 사용하여 amylase의 생산에 미치는 몇가지 요인들을 조사하였다. 본 실험에 사용한 탄소원들중에서 sodium citrate와 왕겨의 효소 생산량이 가장 높았고, 이들을 1:1의 비율로 혼합하여 첨가하였을 때 효소의 생산량은 대조군으로 사용한 가용성 전분에 비하여 20배 이상 증가하였다. 질소원으로 대두박을 사용하였을 경우 yeast extract의 첨가량을 줄이면서 효소의 생산량을 증가시켰다. 배양을 위한 초기 pH를 60으로 조절하고 SDS를 0.01%첨가하였을 때 대조구조보다 높은 효소 생산량을 나타내었다. Bacillus subtilis LKS88 (pASA240)을 sodium citrate와 왕겨 각각 1.5%, K_2HPO_4 0.66%, yeast extract 0.3%, 대두박 0.7%, SDS 0.01% 및 초기 pH를 6.0으로 조절한 배지에서 37℃에서 배양시 최대 효소 활성은 배양 후 36시간에서 나타났으며 이때 효소 활성은 56.6 U/ml이었다. The effects of culture conditions on the production of amylase expressed by Bacillus subtilis LKS88 with a cloned gene from Streptomyces albus KSM-35 were investigated The production of amylase was increased significantly by using sodium citrate and rice hull as a carbon source. In addition, the use of a mixture of sodium citrate and rice hull(1:1) resulted in increase of enzyme production by 20-fold when compared to that of soluble starch. The soybean meal as the nitrogen source could be partially replaced with yeast extract without changing the enzyme production yield. The amylase production was also increased by adjusting initial pH to 6.0 or by adding 0.01% SDS. Maximum amylase production was observed in the medium containing 1.5% sodium citrate, 1.5% rice hull, 0.7% soybean meal, 0.3% yeast extract, 0.66% K_2HPO_4, 0.05% MgSO_4·7H_2O, 0.08% CaCl_2·2H_2O, 0.01% SDS with initial pH of 6.0. The maximum yield of amylase reached 56.6 U/ml when B. subtilis LKS88(pASA 240) was cultured at 37℃ for 36 hr.

      • 2-Methylpyrazinium Chlorochromate를 이용한 치환 벤질알코올류의 산화반응 속도

        최선도,박영조 三陟大學校 産業科學技術硏究所 2003 産業科學技術硏究論文集 Vol.8 No.1

        2-Methylpyrazinium chlorochromate(2-MPC) was synthesized by the reaction of 2-methylpyrazine with chromium(Ⅵ) trioxide in 6M HCI. 2-MPC structure was verified by IR. EA and ICP. The oxidation of benzyl alcohol using 2-MPC in various solvents showed that the reactivity increased with the increase of the dielectric constant's order: cyclohexen < chloroform < acetone < acetonitrile. In the presence of acid. 2-MPC oxidized the substituted benzyl alcohols in N,N-dimethylformamide. The Hammett reaction constant p was -0.68 at 303K. The observed experimental data was used to rationalize the hydride ion transfer in the rate-determining step

      • Dipyrazinium Trichromate에 의한 알코올류의 산화반응에 대한 메카니즘 연구

        최선도,박영조 三陟大學校 産業科學技術硏究所 2004 産業科學技術硏究論文集 Vol.9 No.1

        Dipyrazinium trichromate[(C₄H₄N₂H)₂Cr₃O_(10)) was synthesized by the reaction of pyrazine with chromium(Ⅵ) trioxide in 6M HC1. dipyrazinium trichromate structure was verified by IR, EA and ICP. The oxidation of benzyl alcohol by dipyrazinium trichromate in various solvents showed that the reactivity increased with the increase of the dielectric constant's order: cyclohexen < chloroform < acetone < acetonitrile. In the presence of acid, dipyrazinium trichromate oxidized the substituted benzyl alcohols in N,N-dimethylformamide. The Hammett reaction constant ρ was -0.69 at 303K. The observed experimental data was used to rationalize the hydride ion transfer in the rate-determining step.

      • 성인의 경부 전면 중앙부위에서 발생한 기형종 1예

        최월용,정효진,채종인,하태인,정상렬,이광재,채병도,최영식 고신대학교 의학부 2004 高神大學校 醫學部 論文集 Vol.19 No.1

        Teratomas are embryonal neoplasms that show characteristics of three germ cell layers(ectoderm, mesoderm, and endoderm). They are distributed in the following regions: sacrococcygeal(57%), gonadal(29%), mediastinal(7%), cervical(3%), retroperitoneal(4%), and intracranial(3%) regions. Non-gonadal treatomas present in infancy whereas gonadal are seen in adolescence. Especially, teratomas that occurred in midline neck of adult are very rare and are confused with thyroglossal duct cysts, cystic hygromas, and thyroid tumors. We report, along with the review of appropriate literature, a case of histopathologically confirmed benign teratoma in the midline neck of a 33 years old woman.

      • 한국인 임신부에 있어서 영양소의 역가측정

        최기영,김웅철,박기현,배도환 중앙대학교 의과대학 의과학연구소 1982 中央醫大誌 Vol.7 No.2

        Animal and human studies indicated that inadequate diet for a prolonged period during pregnancy could injure mother, child, or both. In order to prevent this problem, maternal nutyitional status during pregnancy is very important and the evaluation of nutrition during pregnancy have been preceded by many authors in another countries. In Korea, nutrient intake studies of the pregnant women and nutrition surveys in adult by biochemical method have been performed, but there are no data nutrition surveys of Korean pregnant women have been made by biochemical method. In this regard, an exact biochemical titer of the nutrients and the degree of nutrient deficits in Korean pregnant women are needed. In this study, the mean titer of some nutrients and the degree of nutrient deficits by biochemical method in Korean pregnant women were assessed. The objected materials were consisted of 30 cases which had visited the departmant of Obstetrics & Gynecology, Chung Ang University Hospital for antenatal care(32wk-40wk) from March, 1981 to June, 1981. The cases with twin pregnancy, and the cases complicated with materal chronic wasting disease, diabetes, hydroamniosis, hypertensive disorder in pregnancy were excluded in this study. The results were as follows: 1. Mean titer of 6 hours urine execretion of thiamine, riboflavin and N'-methylniacinamide in Korean pregnant women was 44㎍, 466㎍, and 2.33㎎. 2. Mean titer of serum vitamin A, carotene, vitamin C, total protein, calcium, phosphorus, iron and total lipid was 48.1㎍, 154.7㎍, 0.8㎎, 7.0g, 9.8㎎ 3.5㎎, 93㎍, and 578㎎. 3. Percentage of nutrient deficits in Korean pregnant women based on the mean titer of Korean adults was as follow; thiamine-20%, N'-methylniacinamide-7%, total protein-5%, calcuim13%, iron-19%. 4. No cases were deficient in riboflavin, carotene, phosphorus, total lipid and the mean titer of riboflavin and carotene in Korean pregnant women were high compared with thw mean titer of Korean adults.

      • 인터넷 환경상에서 의학정보시스템의 하이퍼미디어 설계

        崔成雲,黃榮道 영남이공대학 1997 論文集 Vol.26 No.-

        The designed demonstration in this study is a part of the heart to be a link of the circulation system of the body in the medical information. It represents the multimedia information on the three-dimensional and audio-visual display with the interlacing and streaming which is connected with the complex media part like the text, video, graphic, audio. animation et tetra with the hyperlink.

      • 돼지 신장세포(PK15)의 이종이식 거부반응에 대한 재조합 IL-18의 영향

        최연실,김은미,김영관,박상연,심정현,윤도영,강형식,명평근 충남대학교 생물공학연구소 2004 생물공학연구지 Vol.10 No.1

        Various cytokines including IL-1β, IL-2, IL-4, IL-10, IL-12p40, IFN-γ, and TNF-α have been recently reported to play important roles in both host and donor cell activations during xenotransplantation. Interleukin (IL)-18, a proinflammatory cytokine, can induce IL-4 and IL-13 production in T cells, NK cells, B cells, mast cells, and basophils. IL-18 has the capacity to stimulate innate immunity and both Th1-and Th2- mediated responses, but little is known to involve in the xenograft rejection. In order to investigate the role of IL-18 in xenograft rejection, we transplanted the pig kidney (PK15) cells to C57BL/6 mouse with or without intraperitoneal injection of mouse recombinatant IL-18. It was analyzed the population of T cell, B cell and NK cell in the mice transplanted with PK 15 cells and recombinant IL-18 by flow cytometry. We found that splenic CD3+ T cells were increased in mice injected PK15 cells with recombinant IL-18. These results suggest that recombinant IL-18 is critical in the xenograft rejection by increasing T cell population of the recipients.

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