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Inducible spy Transcription Acts as a Sensor for Envelope Stress of Salmonella typhimurium
Seon Mi Jeong,Hwa Jeong Lee,Yoon Mee Park,Jin Seok Kim,Sang Dae Lee,Iel Soo Bang 한국축산식품학회 2017 한국축산식품학회지 Vol.37 No.1
Salmonella enterica infects a broad range of host animals, and zoonostic infection threatens both public health and the livestock and meat processing industries. Many antimicrobials have been developed to target Salmonella envelope that performs essential bacterial functions; however, there are very few analytical methods that can be used to validate the efficacy of these antimicrobials. In this study, to develop a potential biosensor for Salmonella envelope stress, we examined the transcription of the S. enterica serovar typhimurium spy gene, the ortholog of which in Escherichia coli encodes Spy (spheroplast protein y). Spy is a chaperone protein expressed and localized in the periplasm of E. coli during spheroplast formation, or by exposure to protein denaturing conditions. spy expression in S. typhimurium was examined by constructing a spy-gfp transcriptional fusion. S. typhimurium spy transcription was strongly induced during spheroplast formation, and also when exposed to membrane-disrupting agents, including ethanol and the antimicrobial peptide polymyxin B. Moreover, spy induction required the activity of regulator proteins BaeR and CpxR, which are part of the major envelope stress response systems BaeS/BaeR and CpxA/CpxR, respectively. Results suggest that monitoring spy transcription may be useful to determine whether a molecule particularly cause envelope stress in Salmonella.
원저 : 당뇨가 없고 정상체중을 가진 사람에서 비알코올성 지방간 질환과 대사성 증후군
김선미 ( Seon Mee Kim ),김정아 ( Jeong A Kim ),한지혜 ( Jee Hye Han ),조경환 ( Kyung Hwan Cho ),윤도경 ( Do Kyung Yoon ) 대한비만학회 2006 The Korean journal of obesity Vol.15 No.1
연구 배경: 비만과 제2형 당뇨병은 비알코올성 지방간 질환 (non-alcoholic fatty liver disease, NAFLD)의 잘 알려진 위험인자이기는 하나 이들과 동반되지 않고 NAFLD가 발생하기도 한다. 이에 본 연구에서는 당뇨병이 없는 정상체중을 가진 사람에서 NAFLD의 임상적 특징을 알아보고 대사증후군과의 관련성을 살펴보고자 한다. 방법: 건강 검진자 중 정상체중을 가지고, 당뇨병이 없으며 간기능이 정상인 NAFLD 환자 (남자 132명, 여자 76명)와 이들과 성별, 연령과 체질량지수를 짝짓기한 정상인을 대상으로 하였다. 이들에서 신체계측, 생화학적 검사와 인슐린 저항성을 측정하여 비교하였고 NAFLD와 관련 있는 대사증후군 요소를 분석하였다. 결과: 남, 여 모두에서 NAFLD군이 대조군에 비해 중성지방, ALT, 인슐린, HOMA-IR이 유의하게 높았고 HDL-콜레스테롤이 유의하게 낮았고 남자에서는 또한 허리둘레, 총콜레스테롤, 공복 혈당, AST가 유의하게 높았다. 단변량 로지스틱 회귀분석 결과 HDL-콜레스테롤 (OR, 95% CI; 1.86, 1.26~2.75), 중성지방 (3.24, 2.13~4.94), 식전 혈당 (2.44, 1.36~4.27), 허리둘레 (1.71, 1.09~2.67), HOMA-IR (4.89, 2.94~8.13), 대사증후군 (3.58, 2.12~6.04)이 NAFLD의 발생과 유의하였으나 다변량 다중 로지스틱 회귀분석을 한 결과 중성지방 (2.35, 1.48~3.66)과 HOMA-IR (1.83, 1.18~2.83)만이 통계적으로 유의하게 NAFLD와 관련이 있었다. 결론: 정상 체중이고 당뇨병이 없는 경우라도 NAFLD가 있다면 인슐린 저항성이 높았고 대사증후군의 여러 요소들과 관련이 있었다. NAFLD은 정상 체중인 경우라도 대사성 질환의 예측인자로 여겨진다. Objective: Obesity and type 2 diabetes are well-known risk factorsof the development of nonalcoholic fatty liver disease (NAFLD) and metabolic syndrome. An association of NAFLD and metabolic syndrome has been suggested. The aims of this study were to evaluate the metabolic significance of NAFLD in nonobese, nondiabetic adults and to assess the independent factors associated with NAFLD. Methods: 132 men and 76 women nonobese (body mass index (BMI) < 23), nondiabetic subjects with NAFLD were studied. The control group consisted of 132 healthy men and 76 women who were matched to the NAFLD group in terms of age, sex and BMI. All the subjects had negative serologic findings for hepatitis B and C viruses and had an alcohol intake less than 40 g/day in men and 20 g/day in women. A standard interview, anthropometrics, a biochemical study, and abdominal ultrasonography were conducted. Results: Triglyceride, ALT, insulin and HOMA-IR were higher in subjectswith NAFLD than control group in both sexes, but waist circumference, total cholesterol, fasting blood sugar and AST were significantly higher only in men group. HDL-cholesterol (OR, 95% CI; 1.86, 1.26~2.75), triglyceride (3.24, 2.13~4.94), fasting blood sugar (2.44, 1.36~4.27), waist circumference (1.71, 1.09 ~2.67), HOMA-IR (4.89, 2.94~8.13), and metabolic syndrome (3.58, 2.12~6.04) were related to development of NAFLD by univariate logistic regression analysis. With multiple logistic regression analysis, the odds ratios of having the NAFLD was (2.33, 1.48~3.66) for triglycerixde and (1.83, 1.18~2.83) for HOMA-IR. Conclusions: NAFLD is closely associated with metabolic disorders, even in nonobese, nondiabetic subjects. NAFLD can be considered an early predictor of metabolic disorders.
B 세포에서 Protein Kinase C-β와 Protein Kinase C-δ에 의해 매개된 세포막 CD27 발현 감소
이선영 ( Seon Yeong Lee ),민준기 ( Jun Ki Min ),조미라 ( Mi La Cho ),문영미 ( Young Mee Moon ),김경운 ( Kyoung Woon Kim ),민소연 ( So Youn Min ),조영규 ( Young Gyu Cho ),윤종현 ( Chong Hyeon Yoon ),박성환 ( Sung Hwan Park ),김호연 대한류마티스학회 2006 대한류마티스학회지 Vol.13 No.1
Objective: CD27 is a member of the tumor necrosis factor receptor (TNFR) superfamily and is expressed on T, B, and NK cells. The signaling via CD27 plays pivotal roles in T-T and T-B interaction. CD27 is a useful marker in assessing the number of circulation B cells and B cell subsets because it permits one step identification of the major B cell compartments, CD27- naive and CD27+ memory B cells as well as CD27high plasma cells. We have analyzed the mechanisms underlying the regulation of CD27 expression. Methods: Isolation B cells and Raji cells were cultured with PMA. The levels of cell surface CD27 and CD 27 mRNA were analyzed by FACs staining and RT-PCR. Raji cells were cultured with phorbol 12-myristate 13-acetate (PMA), with or without pretreated shedding inhibitor BB3103 and TAPI-1. sCD27 was measured in culture supernatant by ELISA. Cell lysates were analyzed for PKC isotype activation by Western blot. We used PKC inhibitor Ly379196 and rottlen. Results: Membrane expression of CD27 was down-regulated after PMA stimulation without cytotoxic effect in B cells. Furthermore, PMA treatment could directly reduce CD27 mRNA without intermediate protein synthesis in B cells. In contrast, PMA treatment induced soluble form of CD27 (sCD27), which was shed from the cell surface and was found in PMA treatment B cell culture supernatant. PMA-induced sCD27 proteins were decreased with shedding inhibitor BB3103 and TAPI-1. PMA-induced down regulation of CD27 expressions were quenched with protein kinase C (PKC) inhibitor Staurosporin, PKC-β inhibitor rottlerin and PKC-δinhibitor Ly379196. Conclusion: These data suggest that PMA-induced activation of PKC plays a crucial role in down-regulation of the expression of the CD27 and up-regulation of the shedding of the CD27 in human B cells.
Clinical Features of Waldenstrom Macroglobulinemia in Korea
( Soo Mee Bang ),( Sook Ryun Park ),( Se Hoon Park ),( Eun Kyung Cho ),( Sung Soo Yoon ),( Dong Bok Shin ),( Jae Hoon Lee ),( Seon Yang Park ),( Byoung Kook Kim ),( Noe Kyeong Kim ) 대한내과학회 2004 The Korean Journal of Internal Medicine Vol.19 No.3
원저 : 백서에서 유도한 지방간에서 Peroxisome Proliferator-Activated Receptor-δ의 치료 효과
김선미 ( Seon Mee Kim ),윤도경 ( Do Kyoung Yoon ),양범석 ( Beom Seok Yang ),조경환 ( Kyung Hwan Cho ) 대한비만학회 2005 The Korean journal of obesity Vol.14 No.1
연구배경: 백서에 에탄올 식이와 고지방 식이를 시켜 알코올성 지방간과 비알코올성 지방간을 유발하고 이들에 peroxisome proliferators-activated receptor δ(PPAR-δ)를 투여하여 PPAR-δ 작용제가 백서의 지방간에서 병리학적 소견과 혈액의 생화학적 소견에 어떤 영향을 주는지 알아보고자 하였다. 방법: 대조군, 고지방 식이를 시킨 군, 고지방 식이를 하면서 PPAR-δ 작용제인 L-165041로 치료한 군, 에탄올 식이 Background: Author used the rat model of alcoholic and nonalcoholic liver disease in which feeding dietary with ethanol and high fat result in the development of fatty liver, necrosis, and inflammation. Author used PPAR-δ agonist in study group. Author in
Yi, Seh-Yoon,Han, Seon-Kyu,Park, Mee-Kyung,Yoo, Young-Sook The Korean Society of Toxicogenomics and Toxicopro 2006 Molecular & cellular toxicology Vol.2 No.2
Sphingolipid metabolites regulate many aspects of cell proliferation, differentiation, and apoptosis. In the present study, we have assessed the effects of the novel phytosphingosine derivative, N-acetylphytospingosine (NAPS), on the depigmentation of murine B16F10 melanoma cells, and have also attempted to identify the possible signaling pathway involved, in comparison with $C_{2}-ceramide$. NAPS and $C_{2}-ceramide$ both inhibited the growth of the B16F10 cells in a dose-dependent manner. Melanin content and tyrosinase activity were significantly reduced in response to treatment with NAPS and $C_{2}-ceramide$ at concentrations in a range between $1-5\;{\mu}M$. However, the levels of tyrosinase mRNA, as well as the levels of tyrosinase related protein-1 (TRP-1) and tyrosinase related protein-2 (TRP-2) genes and the level of tyrosinase protein remained unaffected by treatment with either NAPS or $C_{2}-ceramide$. We also attempted to determine the signaling pathway exploited by NAPS and $C_{2}-ceramide$. Interestingly, the phosphorylation of Akt/PKB at serine 473 by NAPS was reduced at the 5 minute mark, whereas $C_{2}-ceramide$ induced the phosphorylation of Akt/PKB at serine 473. Finally, Akt/PKB activity in the NAPS-treated cells was elevated in comparison with the untreated cells. LY294002, a specific PI3-K inhibitor which is located upstream of Akt/PKB, inhibited the phosphorylation of Akt/PKB, but induced an increase in melanin synthesis. These results suggest that the activation of Akt/PKB at serine 473 is related with the suppression of melanin production in the B16F10 mouse melanoma cells. Therefore, the mechanisms exploited by NAPS and $C_{2}-ceramide$ responsible for the depigmentation of B16F10 cells were concluded to involve the inhibition of melanosomal tyrosinase activity.