하계 실내 및 실외환경의 공기 중 휘발성 유기화합물 농도 및 개인노출

양원호,손부순,박종안,장봉기,박완모,김윤신,어수미,윤중섭,류인철 한국환경과학회 2003 한국환경과학회지 Vol.12 No.9

Volatile organic compounds (VOCs) are present in essentially all natural and synthetic materials from petrol to flowers. In this study, indoor and outdoor VOCs concentrations of houses, offices and internet-cafes were measured and compared simultaneously with personal exposures of each 50 participants in Asan and Seoul, respectively. Also, factors that influence personal VOCs exposure were statistically analyzed using questionnaires in relation to house characteristics, time activities, and health effects. All VOCs concentrations were measured by OVM passive samplers (3M) and analyzed with GC/MS. Target pollutants among VOCs were Toluene, o-Xylene, m/p-Xylene, Ethylbenzene, MIBK, n-Octane, Styrene, Trichloroethylene, and 1,2-Dichlorobenzene. Indoor and outdoor VOCs concentrations measured in Seoul were significantly higher than those in Asan except Ethylbenzene. Residential indoor/outdoor (I/O) ratios for all target compounds ranged from 0.94 to 1.51 and I/O ratios of Asan were a little higher than those of Seoul. Relationship between personal VOCs exposure, and indoor and outdoor VOCs concentrations suggested that time-activity pattern could affect the high exposure to air pollutant. Factors that influence indoor VOCs level and personal exposure with regard to house characteristics in houses were building age, inside smoking and house type. In addition insecticide and cosmetics interestingly affected the VOCs personal exposure. Higher exposure to VOCs might be caused to be exciting increase and memory reduction, considering the relationship between measured VOCs concentrations and questionnaire (p<0.05).

출아효모에서 TAR 클론닝법을 이용한 고등동물의 게놈으로부터 특정 염색체 부위의 분리

박정은,윤영호,이윤주,김광섭,윤세련,안태진,임선희,선우양일 동아대학교 기초과학연구소 2003 基礎科學硏究論文集 Vol.20 No.1

복잡한 게놈 분석에 용이하도록 효모의 인공 염색체(YAC) 클론닝 시스템은 발달되어왔다. YAC은 박테리아의 인공 염색체(BAC)보다 더 큰 단편을 클론닝할 수 있고, 또한 클론된 단편을 쉽게 변형시킬 수 있다. 형질전환과 연계된 재조합법(Transformation-Associated Recombination; TAR)은 게놈 라이브러리를 만들지 않고 직접 게놈 DNA로부터 분리하고자 하는 유전자나 특정 염색체 부분을 클론닝 할 수 있는 방법이다. 이 방법은 spheroplast transformation을 수행하는 동안, 목적으로 하는 유전자의 5' 그리고 3' 염기 배열(hooks)을 지닌 TAR 벡터와 게놈 DNA 사이에서 일어나는 상동성 재조합에 의해 이루어진다. 효모 내의 in vivo 재조합을 이용한 TAR 클론닝법은 복잡한 게놈으로부터 목적의 염색체 부분을 원형 YAC의 형태로서 선택적으로 분리할 수 있다. 그러므로 TAR 클론닝 법은 특정 염색체로부터 YACs을 만드는데 매우 유용하여, 전체 게놈으로부터 특이적 유전자나 유전자의 family를 분리하는데 효과적인 방법으로 사료된다. Yeast artificial chromosome (YAC) cloning systems have advanced the analysis of complex genomes considerably. They permit the cloning of larger fragments than do bacterial artificial chromosome systems, and the cloned material is more easily modified. Transformation-associated recombination (TAR) is a cloning technique that allows specific chromosomal regions or genes to be isolated directly from genomic DNA without prior construction of a genomic library. This technique involves homologous recombination during spheroplast transformation between genomic DNA and a TAR vector that has 5' and 3' gene targeting sequences (hooks). Using in vivo recombination in yeast, TAR cloning selectively isolates, as circular YACs, desired chromosome segments or entire genes from complex genomes. We propose that TAR cloning can provide an efficient means for generating YACs from specific chromosomes and that TAR cloning may be useful for isolating families of genes and specific genes from total genome DNA.

PLD 법으로 제작한 In₂O₃-ZnO 박막의 광학적 및 전기적 특성

신현호(Hyun Ho Shin),한정우(Jung Woo Han),강성준(Seong Jun Kang),윤영섭(Yung Sup Yoon) 대한전자공학회 2008 電子工學會論文誌-SD (Semiconductor and devices) Vol.45 No.7

본 연구에서는 펄스 레이저 법으로 200 mTorr 의 산소 분압에서 기판 온도를 200 ℃ 에서 600 ℃ 까지 변화시켜 가며, quartz 기판 위에 In₂O₃-ZnO 박막을 제작하여 광학적 및 전기적 특성을 조사하였다. XRD 측정을 통해 In₂O₃-ZnO 박막이 다결정 상태인 것을 알 수 있었으며, 기판 온도가 500 ℃ 로 증가함에 따라 35.5° 부근의 In₂O₃(400) 피크는 감소한 반면 30.6°부근의 In2O3 (222) 피크는 증가했다. 박막의 표면을 AFM 으로 조사한 결과, round type 의 결정립들이 관찰되었으며 표면 거칠기 값은 500 ℃ 에서 제작한 박막에서 가장 낮은 값 (6.15 ㎚) 을 나타내었다. 모든 In₂O₃-ZnO 박막이 가시광 영역에서 평균 82 % 이상의 투과율을 보였다. 또, 500 ℃ 에서 제작한 In2O3-ZnO 박막에서 가장 높은 캐리어 농도 (2.46×10<SUP>20</SUP> ㎝<SUP>-3</SUP>) 값과 가장 낮은 비저항 (1.36×10<SUP>-3</SUP> Ω㎝) 값을 나타내었다. In this study, In₂O₃-ZnO thin films are prepared on quartz substrates by the pulsed laser deposition and their optical and electrical properties are investigated as the function of substrate temperatures (200 ~ 600 ℃) at the fixed oxygen pressure of 200 mTorr. The XRD measurement shows that polycrystalline In₂O₃-ZnO thin films are formed. In the XRD measurement, the intensity of the (400) In₂O₃ peak at 35.5° decreases and that of the (222) In₂O₃ peak at 30.6° increases with the increase substrate temperature up to 500 ℃. From the result of AFM measurement, the morphology of In₂O₃-ZnO thin films are observed as round-type grains. The lowest surface roughness (6.15 ㎚) is obtained for the In₂O₃-ZnO thin film fabricated at 500 ℃. The optical transmittance of In₂O₃-ZnO thin films are higher than 82 % in the visible region. The maximum carrier concentration of 2.46×10<SUP>20</SUP> ㎝<SUP>-3</SUP> and the minimum resistivity of 1.36×10<SUP>-3</SUP> Ω㎝ are obtained also for the In₂O₃-ZnO thin film fabricated at 500 ℃.

갑상선 선지방종 1예

김형일,홍순원<SUP>1<.SUP>,김은경<SUP>2<.SUP>,윤종호<SUP>3<.SUP>,박정수,Hyoung-Il Kim,M.D.,Soon Won Hong,M.D.<SUP>1<.SUP>,Eun Kyung Kim,M.D.<SUP>2<.SUP>,Jong Ho Yoon,M.D.<SUP>3<.SUP> and Cheong Soo Park,M.D. 대한갑상선-내분비외과학회 2004 The Koreran journal of Endocrine Surgery Vol.4 No.1

Adenolipoma of the thyroid gland, also called thyrolipoma, is an extremely rare neoplasm characterized by encapsulated or tumorous mass of admixtures of mature adipose tissue and thyroid tissue. Only a few scattered cases have been reported in English literatures. It should be differentiated with encapsulated papillary carcinoma, follicular neoplasm and liposarcoma. We report an additional case of an adenolipoma of the thyroid gland. (Korean J Endocrine Surg 2003;4:51-54)

동종 아킬레스건을 이용한 경경골 단일 후방 십자 인대 재건술과 이중 다발 후방 십자 인대 재건술의 임상적, 방사선학적 결과 비교 : 최소 2년 추시

윤여승(Yeo-seung Yoon),김두섭(Doo-sup Kim),전종세(Jong-se Jeon),강동현(Dong-hyun Kang),이창호(Chang-ho Lee) 대한정형외과학회 2010 대한정형외과학회지 Vol.45 No.4

목적: 동종 아킬레스건을 이용하여 경경골 단일 후방 십자 인대 재건술과 이중 다발 후방 십자 인대 재건술을 시행하고 임상적. 방사선학적 결과를 비교하였다. 대상 및 방법: 2000년 2월부터 2007년 6월까지 본원에서 후방 십자 인대 재건술을 시행하고 2년 이상 추시가 가능하였던 41예 중단일 후방 십자 인대 재건술 22예(53.7%), 이중 다발 후방 십자 인대 재건술 19예(46.3%)를 대상으로 하였다. 임상결과는 Lysholm 슬관절 점수, IKDC scale 방사선학적 결과는 Telos<SUP>®</SUP> 후방 전위 검사를 시행하여 비교하였다. 결과: 단일 후방 십자 인대 재건술 군은 Lysholm 점수가 술 전 평균 52.7±3.9점 최종 추시 시 평균 85.4±4.7점, IKDC scale은 술 전 grade C8예(36.3%), D 14예(63.6%), 술 후 grade A 4예(18.2%), B 14예(63.6%), C 4예(18.2%) 보였으며, 이중 다발 후방 십자 인대 재건술군은 Lysholm 점수가 술 전 평균 50.4±7.4점, 최종 추시 시 평균 87.5±5.12점 IKDC scale은 술 전 grade C 4예(21.1%), D 15예(78.9%). 최종 추시 시 grade A 1예(5.3%), B 15예(78.9%), C 3예(15.8%)였다. 최종 추시 시 Telos<SUP>®</SUP> 후방 전위 검사는 단일 후방 십자 인대 재건술군은 술 전 평균 14.6±2.52 ㎜ (10-19 ㎜), 최종 추시 시 평균 5.06±2.07 ㎜ (3.04-7.43 ㎜), 이 중 다발 후방 십자 인대 재건술 군에서는 술 전 평균 16.5±3.84 ㎜ (12-22 ㎜), 최종 추시 시 평균 4.04±1.48 ㎜ (2.18-7.20)㎜로 임상적. 방사선적 평가상 두 군 모두 술 전보다 의미있게 향상되었으나(p=0.001) 두 군 간의 통계학적 유의성은 없었다(p=0.458). 결론: 저자들은 이중 다발 후방 십자 인대 재건술을 시행하고 임상적 결과 및 방사선학적 검사의 결과를 단일 후방 십자 인대 재건술을 시행한 군과 비교하였으나 단기 추시 상에서는 두 군 간의 유의한 차이는 보이지 않아 장기 추시상의 결과 비교가 필요하리라 생각된다. Purpose: We performed transtibial single and double bundle reconstruction of the posterior cruciate ligament using the alia-achilles tendon and compared the results of single bundle reconstruction and double bundle reconstruction both clinically and radiologically. Materials and Methods: This study included 41 cases of posterior cruciate ligament reconstruction between February 2000 and June 2007 that had data available for at least 2 years of follow-up. Twenty-two cases (53.7%) underwent single bundle reconstruction and 19 cases (46.3%) underwent double bundle reconstruction. Clinical results were analyzed by Lysholm score and IKDC standards scale, and the radiologic results were analyzed by the Telos<SUP>®</SUP> posterior translation test. Results: The average Lysholm score at last follow-up was 85.4±4.7 in the single bundle group and 87.5±5.1 in the double bundle group. IKDC scores were grade A in 4 (18.2%). grade B in 14 (63.6%) and grade C in 4 (18.2%) in the single bundle group; they were 1 (15.3%), 15 (78.9%) and 3 (15.8%) in the double bundle group. Telos<SUP>®</SUP> posterior translation test scores were 5.06 ㎜ (3.04-7.43 ㎜) in the single bundle group, and 4.04 ㎜ (2.18-7.20 ㎜) in the double bundle group. There was significant improvement in clinical and radiological scores in both groups (p<0.001). However, these differences were not statistically significant (p>0.05). Conclusion: Further study with longer term follow-up is needed.

부갑상선 낭종의 진단적 접근 및 치료

윤종호<SUP>1<.SUP>,최선호,남기현,장항석,박정수,Jong Ho Yoon,<SUP>1<.SUP>,Sun Ho Choi,Kee-Hyun Nam,Hang-Seok Chang,and Cheong Soo Park 대한갑상선-내분비외과학회 2004 The Koreran journal of Endocrine Surgery Vol.4 No.2

순국선열을 기리는 소극장 계획 연구

윤춘섭(Yoon, Choon-Sup),양정호(Yang, Jeong-Ho) 대한건축학회 2016 대한건축학회 학술발표대회 논문집 Vol.36 No.2

The aim of this study is to commemorate the souls of patriots who sacrificed their lives for the independence of the country under the regime of Japanese rule in early 20<SUP>th</SUP> century. For this purpose, a small theater was proposed to commemorate them. The program of theater includes drama, education and exhibition. To emphasize the significance of the theater, the location was decided to be adjacent to Seodaemun prison and a neighborhood called Okbaraji, in which the families of patriotic prisoners had lived to support them.

Comparative proteomic analysis of the insulin-induced L6 myotube secretome

Yoon, Jong Hyuk,Yea, Kyungmoo,Kim, Jaeyoon,Choi, Yoon Sup,Park, Sehoon,Lee, Hyeongji,Lee, Chang Sup,Suh, Pann-Ghill,Ryu, Sung Ho WILEY-VCH Verlag 2009 Proteomics Vol.9 No.1

<P>Emerging evidence has revealed an endocrine function for skeletal muscle; in fact, certain anti-inflammatory cytokines are secreted only from contractile skeletal muscle. However, the skeletal muscle secretome as a whole is poorly characterized, as is how it changes in response to extracellular stimuli. Herein, we sought to identify and characterize the members of the skeletal muscle secretome, and to determine which protein secretion levels were modulated in response to insulin stimulation. To conduct these studies, we treated differentiated L6 rat skeletal muscle cells with insulin or left them untreated, and we comparatively analyzed the proteins secreted into the media. We fractionated this conditioned media using offline RP HPLC, digested the fractionated proteins, and analyzed the resulting peptides with LC-ESI-MS/MS. We identified a total of 254 proteins, and by using three different filtering methods, we identified 153 of these as secretory proteins. Fourteen proteins were secreted at higher levels under insulin stimulation, including several proteins known to be highly secreted in metabolic diseases; 19 proteins were secreted at lower levels under insulin stimulation. These result not only pinpointed several previously unknown, insulin induced, secretory proteins of skeletal muscle, it also described a novel approach for conditioned secretome analysis.</P>

Anti-inflammatory Effects of Ethanolic Extracts from Codium fragile on LPS-Stimulated RAW 264.7 Macrophages via Nuclear Factor kappaB Inactivation

Yoon, Ho-Dong,Jeong, Eun-Ji,Choi, Ji-Woong,Lee, Min-Sup,Park, Myoung-Ae,Yoon, Na-Young,Kim, Yeon-Kye,Cho, Deuk-Moon,Kim, Jae-Il,Kim, Hyeung-Rak The Korean Society of Fisheries and Aquatic Scienc 2011 Fisheries and Aquatic Sciences Vol.14 No.4

Bacterial lipopolysaccharide (LPS) induces expression of pro-inflammatory cytokines and enzymes producing nitric oxide (NO) and prostaglandins (PGs) in immune cells. This process is mediated by the activation of nuclear factor kappaB (NF-${\kappa}B$). In this study, we investigated the anti-inflammatory characteristics of Codium fragile ethanolic extract (CFE) mediated by the regulation of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) using LPS-stimulated murine macrophage RAW 264.7 cells. CFE significantly inhibited LPS-induced NO and $PGE_2$ production in a dose-dependent manner and suppressed the expression of iNOS and COX-2 proteins in LPS-stimulated RAW 264.7 cells with no cytotoxicity. Pro-inflammatory cytokines, such as interleukin (IL)-$1{\beta}$, IL-6, and tumor necrosis factor-${\alpha}$, were significantly reduced by treatment of CFE in LPS-stimulated RAW 264.7 cells. CFE inhibited the promoter activity of (NF)-${\kappa}B$ in LPS-stimulated macrophages. Treatment with CFE suppressed translocation of the NF-${\kappa}B$ p65 subunit by preventing proteolytic degradation of inhibitor of ${\kappa}B-{\alpha}$. These results indicate that the CFE-mediated inhibition of NO and $PGE_2$ production in LPS-stimulated RAW 264.7 cells is mediated through the NF-${\kappa}B$-dependent transcriptional downregulation of iNOS and COX-2, suggesting the potential of CFE as a nutraceutical with anti-inflammatory activity.

The Study on Validity for Estimation of Maximal Oxygen Uptake through PWC%HRR during Arm Ergometer Exercise

Yoon, Ho-Sup,Ryoo, Byung-Kwan,Jung, Deog-Jo,Park, Sung-Tae,Kang, Hyun-Joo,Kim, Eun-Hye 한국체육무용국제교류학회 2000 한국체육무용국제교류학회지 Vol.6 No.-

The purpose of this study was to evaluate the validity for estimation of maximal oxygen uptake through PWC%HRR during Arm Ergometer Exercise. The subjects of this study were 20 healthy males(21.2± 2077yrs) college students. Resting heart rate of subjects were recorded by EKG for 5min. the maximal oxygen uptake was determined until subjects were exhausted. The first stage was performed 50rpm, 20W, and then was increased by 10W every 3 minutes on the arm ergometer. HRmax was determined by a numerical formula of HRmax = 210_age. PWC%HRR was assessed by relative equation (Y=bX+a) of HR and VO2 observed in range 120 ≤ R ≥ 160 during the exercise test. Range of relative percentage was between 50% and 85%, comparison factor was examined among PWC%HRR, %VO2R and %VO2max. The results of this study are as follows : 1) Result of the regression analysis among %HRR, %VO2R and %VO2max, %HRR was equivalent to %VO2R rather than VO2max. 2) No significant difference was observed among PWC%HRR, %VO2R and %VO2max in the all intensity, But the PWC%HRR was closer to %VO2R rather than VO2max (P<.05). 3) No significant difference was the actual VO2max and the estimation value by PWC%HRR = %VO2R in the all intensity, but the estimation value by PWC%85HRR was the most similar to the actual measurement and the correlation analysis was the most correlated at the estimation value by PWC%60HRR. It was concluded that if the satisfactory VO2max was not found in Arm Ergometer exercise test but heart rate corresponded to 60%HRR, VO2max can be estimated by a series of equation.