환경, 에너지 그리고 순환 : 포스터 ; 대기실태 및 관리 : 서울시 다중이용시설 휘발성유기화합물(VOCs) 분포특성

류인철,이호찬,조현석,이진,전재식,김주형 한국폐기물자원순환학회 2007 2007 환경공동학술대회 Vol.2007 No.1

홍화 추출물이 치주인대세포, 조골세포 활성도에 미치는 영향

류인철,이용무,구영,배기환,정종평,Rhyu, In-Chul,Lee, Yong-Moo,Ku, Young,Bae, Ki-Whan,Chung, Chong-Pyoung 대한치주과학회 1997 Journal of Periodontal & Implant Science Vol.27 No.4

Safflower(Carthamus tinctorius $Linn\acute{e}$ has been traditionally used for the treatment of blood stasis, and Dipsasi Radix has been used as a drug for fracture in Chinese medicine. The purpose of present study was to examine the biologic effects of safflower extract and Disasi radix extracts on the periodontal. ligament cells and osteoblastic cells and on the wound healing of rat calvarial defect. The ethanolic extract of safflower blossom, safflower seed and Dipsasi Radix(125, 250, and 500 ${\mu}g/ml$) were prepared as test group, and PDGF-BB(lOng/ml) and unsafonifiable fraction of Zea Mays L.(125, 250, and 500 ${\mu}g/ml$) were employed as positive control. The effects of each agents on the growth and survival, ALPase activity, expression of PDGF-BB receptor, chemotactic response of PDL cell and ATCC human osteosarcoma MG63 cells in vitro were examined. The tissue regenerative effect of each extracts was evaluated by histomorphometric measuring of newly formed bone on the 8mm defect in rat calvaria after oral administration of 3 different dosages groups : 0.02, 0.1 and 0.35g/kg, per day. It was also employed the same dosages of unsaponifiable fraction of Zea Mays L. as positive controls. Safflower blossom extract, safflower seed extract, and Dipsasi Radix extract stimulate the cellular activity of MG63 cells in concentration range of $125-500{\mu}g/ml$, and safflower bolssom extract and safflower seed extract stimulate also the cellular activity of periodontal ligament cells in concentration range of $250-500{\mu}g/ml$. In activity of ALPase, $250-500{\mu}g/ml$ of safflower blossom extracts showed significant stimulating effects on MG63 cells, and the same concentration range of safflower seed extracts showed significant effect on periodontal ligament cells. In the recovery on PDGF-BB receptor expression which was depressed by $IL-1{\beta}$, $125-250{\mu}g/ml$ of safflower blossom extracts and $250-500{\mu}g/ml$ of safflower seed extracts showed significant increasing effect on MG63 cells, and $500{\mu}g/ml$ of safflower blossom extract and $250-500{\mu}g/ml$ of safflower seed extracts showed significant effect on periodontal ligament cells. In chemotactic response, among all tested group, safflower seed extracts only were chemotactic to MG63 cells and periodontal ligament cells in concentration range of $125-500{\mu}g/ml$. Also in the view of bone regeneration in rat calvarial defect model, the only group that was orally administrated 0.35g/kg, day of safflower seed extract showed significant new bone formation. These results suggested that safflower extracts might have a potential possibilities as an useful drug for adjunct to treatment for regeneration of periodontal defect.

Poly(alpha-hydroxy acids) 제제 생분해성 차폐막의 치주조직 재생유도능력에 관한 조직학적 장기관찰

류인철,구영,정종평,한수부,최상묵,Rhyu, In-Chul,Ku, Young,Chung, Chong-Pyoung,Han, Soo-Boo,Choi, Sang-Mook 대한치주과학회 1997 Journal of Periodontal & Implant Science Vol.27 No.3

The recent trend of research and development on guided tissue regeneration focuses on the biodegradable membranes, which eliminate the need for subsequent surgical removal. They have demonstrated significant and equivalent clinical improvements to the ePTFE membranes. This study evaluate guided tissue regeneration wound healing in surgically induced intrabony periodontal defects following surgical treatment with a synthetic biodegradable membranes, made from a copolymer of glycolide and lactide, in 8 beagle dogs. After full thickeness flap reflection, exposed buccal bone of maxillary and mandibular canine and premolar was removed surgically mesiodistally and occlusoapically at $6mm{\times}6mm$ in size for preparation of periodontal defects. In experimental sites a customized barrier was formed and fitted to cover the defect. Flap was replaced slightly coronal to CEJ and sutured. Plaque control program was initiated and maintained until completion of the study. In 4, 8, 16 and 24 weeks after surgery, the animals were sacrificed and then undecalcified specimens were prepared for histologic evaluation. Histologic examination indicated significant periodontal regeneration characterized by new connective tissue attachment, cementum formation and bone formation. These membranes showed good biocompatibility throughout experiodontal period. The barriers had been completely resorbed with no apparent adverse effect on periodontal wound healing at 24 weeks. These results implicated that present synthetic biodegradable membrane facilitated guided tissue regeneration in periodontal defect.

수종의 상용 세치제들의 향균 및 향염효과 비교연구

류인철,Rhyu, In-Chul 대한치주과학회 1996 Journal of Periodontal & Implant Science Vol.26 No.2

It is known that some natural extracts from plants have a various range of antimicrobial and antiinflammatory activity. There are lots of clinical trials to develop toothpastes containing natural extracts for prevention of dental caries and gingival inflammation. The purpose of this study was to evaluate antimicrobial and antiinflammatory activity of magnolol containing toothpastes and other commercial toothpastes. Eleven kinds oftoothpastes were used. They include magnolol, sanguinarine, Myrrha, Mori radicis cortex,Cimicifugae rhizoma, sodium fluoride, aminocaprolactic acid etc. Six strains of bacteria were used for this test, ego Porphylomonas gingivalis, Prevotellain-termedia, Actinobacillus actinomy cetemcomitans, Streptococcus mutans, Stretococcus sanguis, and Actinomyces species. Antimicrobial activity was determined by an agar dillution method and a broth microdillution method. Antiinflammatory activity was assessed by the inhibition of $PGE_2$ production from gingival fibroblast with the addition of rHIL-1 and centrifuged solution of toothpastes. Control group was only rHIL-1 additive sample. $PGE_2$ enzyme immunoassay systemfAmersham, In. Buckinghamshire, U.K). $PGE_2$ level was measured by ELISA reader with 450 nm, The results from the study revealed that toothpastes containing natural extracts generally had high antimicrobial and antiinflammatory activity. Especially magnolol containing toothpaste showed higher antimicrobial activity than other toothpastes, and sanguinarine containing toothpaste showed particularly high antimicrobial activity in A. actinomicetemcomitans and A. viscosus. In some degree all toothpastes inhibited $PGE_2$ production, but magnolol containing toothpaste was potent inhibitor of $PGE_2$. Sodium chloride containing toothpaste had also effective result. The results suggested that toothpastes containing natural extracts were promising in plaque control and prevention of dental caries and gingivitis.

생약 추출물이 세포성장 및 cytokine 생산에 미치는 영향

류인철,손성희,정종평,배기환,Ryu, In-Cheol,Son, Seong-Heui,Chung, Chong-Pyoung,Bae, Ki-Hwan 대한치주과학회 1993 Journal of Periodontal & Implant Science Vol.23 No.1

The native connective tissue attachment of the periodontium is known to be a complex consisting of gingival fibroblasts, periodontal ligament cells, gingival epithelial cells, cementum, alveolar bone and extensive extracellular matrix (collagen, glycoprotein and proteoglycans). The purpose of this study was to evaluate the effects of natural extracts on DNA, collagen and protein synthesis and inhibition of cytokine production in the gingival and periodontal ligament fibroblasts and gingival epithelial cells. Healthy gingival tissue was obtained from orthodontic treatment patients, and gingival epithelial cells, gingival fibroblasts and periodontal ligament cells were isolated and cultured from the samples. After treated with Ginseng protein, Pluronic F-68, Scutellariae Radix, centella asiatica, PDGF, IGF, DNA synthesis, total protein and collagen synthesis, and cytokine production of gingival epithelial cell, gingival fibroblast and periodontal ligamentcells were measured. MTT method for DNA synthesis, Peterkofsky and Dingerman method for total protein and collagen synthesis, and IL-1 ELISA kit for cytokine production were used. The proliferation of epithelial cells was enhanced in Centella asiatica, Ginseng protein, Pluronic F-68 and Scutellariae Radix. The activities of PDL cells were increased in PDGF, IGF, and Pluronic F-68. Higher collagen synthesis was observed in Scutellariae Radix and total protein synthesis was increased in Scutellariae Radix and PDGF. The inhibitory effects on IL-1, IL-6, $TNF-{\alpha}$ were observed in all exrracts.

SSM과 중소상인 상생 협력 방안의 연구

류인철,강한수,최용석 한국지역발전학회 2012 지역발전연구 Vol.12 No.1

본 연구는 최근 우리나라의 SSM 출점실태 파악과 2010년 국회에서 통과된 SSM 개정법을 검토하여 상생협력 방안을 제시하였는데 다음과 같다. 첫째, 법률적 보호 장치마련이 우선적으로 이루어져야 한다. 현재 일선 자치단 체와 대형마트간의 영업규제를 둘러싼 대립이 뚜렷한 해결책을 찾지 못하자 법 으로 이를 강제하려는 움직임이 현실화되고 있다. 상위 법률이 강화되고, 일선 자치단체들의 관련 조례도 법률적 허점을 보완한다면 대형마트 등의 영업시간 제한은 법률적으로 가능해진다. 둘째, 지역상권의 자구노력이 필요하다. 지역상권의 자구노력 방안으로는 시설 의 현대화 지원, 소비자와 호흡하는 서비스 창출, 차별화 정책, 상인들의 의식개 선과 더불어 지역주민들의 의식변화도 필요, 경쟁력 제고를 위한 상인의 역할과 의지, 전통시장 미를 살리는 것 등이다. 셋째, 대형마트의 자발적 휴무가 필요하다. 법과 조례 등 강제성으로 휴무를 하 는 것보다 대형마트의 자발적인 휴무가 필요하다. 넷째, 대형마트가 지역사회에 공헌해야 한다. 수백억대의 연간매출을 올리는 대형마트 가 지역사회에 장학사업, 사회봉사사업, 지역 문화예술지원 사업, 지역상 품 판로확대 사업 등의 방법으로 공헌해야 한다. 다섯째, 대형마트의 사회적 책임 수용이 필요하다. 일선 자치단체와 사회단체 등을 중심으로 대형마트를 대상으로 지역주민 정규직 고용, 지역 업체 입점, 지 역 중소기업 제품구매, 현금 매출액 지역은행 예치, 대형마트 현지법인화, 연간 총매출의 일정액 지역 환원, 지역상권 활성화 부담금 강제부과 등 상생방안이 논의되고 있으나, 이를 법률적으로 시행하기는 어려운데, 결국, 대형마트 등이 사회적 책임 차원에서 이를 수용하지 않으면 실행은 불가능하다. 여섯째, 현지 법인화가 추진되어야 한다. 법인화가 추진되면 기업경영에 필요한 모든 요소를 독립적으로 수행하기 때문에 인재채용, 협력업체 육성, 지역상품 판로확대 면에서 지역에 기여하는 바가 클 수밖에 없다. 또한 지역자금의 역외 유출 방지와 지역 농수산물 구입, 지역인재 채용 등으로 지역경제 활성화에 큰 도움이 될 것이며, 지역자금의 역외유출 방지와 지역 농수산물 구입, 지역인재 채용 등으로 지역경제 활성화에 큰 도움이 될 것이다. 이상과 같이 현재 대형마트와 재래시장 사이의 갈등은 끝이 보이지 않는다. 대 형마트 규제가 속속 법제화되고 있지만, 대형마트측은 법원소송까지 제기하며 거세게 반발하고 있는 형국이다. 재래시장과 소상공인들은 신규입점 및 영업시 간 규제에도 불구하고, 추가 규제를 요구하고 있다. 과연 대형마트와 중소상인과 상생 협력하는 방법은 규제보다는 서로 공생할 수 있는 방안을 모색하는 것이 가장 중요하다.

흡수성 차폐막의 치주조직 재생에 혈소판유래 성장인자가 미치는 영향

류인철,배규현,설양조,구영,이승진,한수부,최상묵,정종평,Rhyu, In-Chul,Bae, Kyoo-Hyun,Seol, Yang-Jo,Ku, Young,Lee, Seung-Jin,Han, Soo-Boo,Choi, Sang-Mook,Chung, Chong-Pyoung 대한치주과학회 1999 Journal of Periodontal & Implant Science Vol.29 No.3

The ultimate objective of periodontal treatment is to stop disease progression and to regenerate destroyed periodontal tissues and thereby regain normal function. Growth factors are naturally found polypetides which stimulate many cellular activities pertaining to wound healing by acting as signal molecule in controlling cell movement, proliferation, and matrix production. Platelet derived growth factor (PDGF) is 28,000-35,000 Da molecular weight dimeric protein with 2 long positively charged polypeptide chains connected by sulfide bonds. The purpose of this study is to evaluate histologically the initial guided tissue regeneration in a periodontal defect f a beagle dog treated with a biodegradable membrane formed with polylactic acid (poly-L-lactic acid) and polyglycolic acid loaded with 200ng/$cm^2$ platelet derived growth factor. 2 beagle dogs were used in he experiment. $5mm{\times}6mm$ alveolar bone defect was formed in upper and lower canines and third premolars and a reference notch was placed. PDGF-BB non-containing membrane was used as control. Each defect was randomly assigned to the test roup or the control group. The dogs were sacrificed 3 weeks after membrane placement. Toluidine blue and multiple staining was done for histological analysis. In the 3 week specimen in the control group, no new one formation could be seen. Small amount f bone resorption below the notch could be seen. In the notch, loose connective tissue with infiltration of inflammatory cells could be seen. Also thin discontinuous new cementum could be seen and the membrane still retained its structure. Where PDGF-BB containing membrane was used, new bone formation could be seen in the notch at weeks and also continuous thin cementum could be seen. PDL cells were observed between new bone and new cementum and some were attached to bone and cementum. These results suggest that new bone and cementum formation seen when PDGF-BB loaded membrane was used was due to inhibition of downgrowth of epithelial cells and also due to continuous release of the growth factor. Further study on the resorption characteristics of the membrane nd the release characteristics of the PDGF-BB is necessary. Also, development of a membrane easier to use clinically is necessary.

골유도재생술식의 이해와 적용

류인철 대한치과의사협회 2005 대한치과의사협회지 Vol.43 No.12

Tixocortol pivalate를 함유한 Chlorhexidine이 치은연하 치태세균 및 치은 각화에 미치는 영향

류인철,손성희,정종평,Rhyu, In-Cheol,Son, Seong-Heui,Chung, Chong-Pyoung 대한치주과학회 1985 Journal of Periodontal & Implant Science Vol.15 No.1

서울시 다중이용시설 휘발성유기화합물(VOCs) 분포특성

류인철,이호찬,조현석,이진,전재식,김주형 대한환경공학회 2007 대한환경공학회 학술발표논문집 Vol.2007 No.12