배추 시스테인 단백질 분해 효소 억제 유전자 BCPI-1의 발현과 종자 발아 및 유묘 생장과의 연관성

홍준기,이은영,김정률,양경애,최영주,정우식,김호일,윤대진,이상열,조무제,임체오 Plant molecular biology and biotechnology research 2003 Plant molecular biology and biotechnology research Vol.2003 No.-

Phytocystatins are protein inhibitors of cysteine proteinases of the papain family that have been identified in both monocot and dicot plants. A cDNA encoding a phytocystatin, BCPI-1 (Brassica Cysteine Proteinase Inhibitor-1) has been isolated from Chinese cabbage (Brassica campestris L. ssp. pekinensis) flower buds. Here, we tested whether BCPI-1 transcription is regulated by hormones, and could be involved in regulating cysteine proteinases during and after germination. BCPI-1 was sensitive to exogenous GA_(3) and ABA, which are important factors controlling seed germination, suggesting that the expression of BCPI-1 is hormonally regulated. We introduced a recombinant plasmid containing the full-length BCPI-1 cDNA under the control of cauliflower mosaic virus 35S promoter into rice embryogenic calli using the particle delivery method, and regenerated a number of transgenic rice plants. Constitutively over-expressed BCPI-1 caused changes in overall plant growth and development, including reduced germination and seedling growth. These data support the role of the BCPI-1 in the regulation of endogenous proteinases during both seed germination and subsequent seedling development. Phylocystatins은 papain계열의 cysteine 단백질 분해 효소 활성을 특이적으로 억제하는 억제자로 다양한 식물 종으로부터 분리되었다. 본고에서는, 배추 화아 cDNA library로부터 분리된 phytocystatin인 BCPI-1(Brassica Cysteine Proteinase Inhibitor-1)의 생체 내 기능에 대해 연구하였다. 먼저, BCPI-1 전사체는 발아 조절에 중요한 영향을 미치는 GA₃와 ABA에 의해 예민하게 증가, 혹은 감소되는 반응을 보임으로써, BCPI-1이 식물 호르몬의 영향을 받으며, 특히 발아나 유묘의 생장 조절에 관련이 있음을 알 수 있었다. Particle bombardment 방법을 통하여 BCPI-1을 벼의 배 형성 세포 내에 도입, 재분화 시켜 형질 전환 벼를 생성하였다. CaMV 35S promoter의 조절에 의해 지속적인 BCPI-1 발현을 보이는 형질 전환 벼의 경우, 발아와 유묘 생장이 현저히 지연되었다. 위의 결과들을 바탕으로, BCPI-1이 식물체 내에서 생성되는 cysteine 계열의 단밸질 분해 효소 활성을 조절하여 종자의 발아와 유묘의 생장에 영향을 미치는 것으로 추측한다.

Applying Artificial Neural Network to Fault Diagnosis of Analogue Circuit

Yang, ZhEng Rong,Musgrave, G. 대한전자공학회 1993 ICVC : International Conference on VLSI and CAD Vol.3 No.1

In this paper, we have applied artificial neural networks to fault diagnosis of analogue circuits. The experiments presented have proved that the artificial neural network can be used both for catastrophic faults and parametric faults. We have applied three artificial neural network, models to the fault diagnosis of DC, frequency, parametric, and steady state response. Two important techniques are introduced for the selection of artificial neural network models. These two techniques are: pattern distinction and quantity factor graph.

从《倩女幽魂》到《九层妖塔》

??(Yang Zheng) 중국영화포럼 2016 영화중국 Vol.3 No.1

Since the 1990s, Chinese culture and society resulted in many changes, Chinese Monster films had shown a history of promoting hidden behind the text in context. By combing the period from Ghost Story to Chronicles of the Ghostly Tribe, monster image’s changes in Chinese monster films, this paper argues that the Chinese monster film increasing emphasis on the story of the text and the ideological content of will become the new trend.

Review : Production and Characterization of Monoclonal and Recombinant Antibodies Against Antimicrobial Sulfamethazine

( Zheng You Yang ),( Won Bo Shim ),( Min Gon Kim ),( Kyu Ho Lee ),( Keun Sung Kim ),( Kwang Yup Kim ),( Cheol Ho Kim ),( Sang Do Ha ),( Duck Hwa Chung ) 한국미생물 · 생명공학회 2007 Journal of microbiology and biotechnology Vol.17 No.4

The effect of SiO2 on the performance of inorganic sludge-based structural concretes

Zheng Xun Yang,Na Ra Ha,이종국,Min Sik Jang,황규홍 한양대학교 세라믹연구소 2009 Journal of Ceramic Processing Research Vol.10 No.3

As a relatively new material, geopolymer concrete offers benefits as a construction material for sustainable development. It utilizes waste materials such as recycled concrete sludge, fly ash, etc. It has a very low rate of green house gas emission when compared to ordinary Portland cement. In this study, the components of geopolymer are concrete sludge, metakaolin and water glass. NaOH was used as an alkaline activator. To improve the mechanical properties, silica fume was added as a bonding matrix from 0%-10% to replace part of the concrete sludge, and the specimens were cured in air, then their mechanical properties such as compressive strength and bending strength were measured and their microstructures were investigated.

Biomechanical Evaluation of 2 Endoscopic Spine Surgery Methods for Treating Lumbar Disc Herniation: A Finite Element Study

Yang Zou,Shuo Ji,Hui Wen Yang,Tao Ma,Yue Kun Fang,Zhi Cheng Wang,Miao Miao Liu,Ping Hui Zhou,Zheng Qi Bao,Chang Chun Zhang,Yu Chen Ye 대한척추신경외과학회 2024 Neurospine Vol.21 No.1

Objective: This study aimed to evaluate the effects of 2 endoscopic spine surgeries on the biomechanical properties of normal and osteoporotic spines. Methods: Based on computed tomography images of a healthy adult volunteer, 6 finite element models were created. After validating the normal intact model, a concentrated force of 400 N and a moment of 7.5 Nm were exerted on the upper surface of L3 to simulate 6 physiological activities of the spine. Five types of indices were used to assess the biomechanical properties of the 6 models, range of motion (ROM), maximum displacement value, intervertebral disc stress, maximum stress value, and articular protrusion stress, and by combining them with finite element stress cloud. Results: In normal and osteoporotic spines, there was no meaningful change in ROM or disc stress in the 2 surgical models for the 6 motion states. Model N1 (osteoporotic percutaneous transforaminal endoscopic discectomy model) showed a decrease in maximum displacement value of 20.28% in right lateral bending. Model M2 (unilateral biportal endoscopic model) increased maximum displacement values of 16.88% and 17.82% during left and right lateral bending, respectively. The maximum stress value of L4–5 increased by 11.72% for model M2 during left rotation. In addition, using the same surgical approach, ROM, maximum displacement values, disc stress, and maximum stress values were more significant in the osteoporotic model than in the normal model. Conclusion: In both normal and osteoporotic spines, both surgical approaches were less disruptive to the physiologic structure of the spine. Furthermore, using the same endoscopic spine surgery, normal spine biomechanical properties are superior to osteoporotic spines.

Three-Dimensional Elastic Stress Fields of Finite Thickness Plates with Elliptical Hole

Yang, Zheng,Cho, Chong Du,Su, Ting Ya,Kim, Chang Boo,Beom, Hyeon Gyu Trans Tech Publications, Ltd. 2007 Key Engineering Materials Vol.353 No.-

<P>Based on detailed three-dimensional finite element analyses, elastic stress and strain field of ellipse major axis end in plates with different thickness and ellipse configurations subjected to uniaxial tension have been investigated. The plate thickness and ellipse configuration have obvious effects on the stress concentration factor, which is higher in finite thickness plates than in plane stress and plane strain cases. The out-of-plane stress constraint factor tends the maximum on the mid-plane and approaches to zero on the free plane. Stress concentration factors distribute ununiformly through the plate thickness, the value and location of maximum stress concentration factor depend on the plate thickness and the ellipse configurations. Both stress concentration factor in the middle plane and the maximum stress concentration factor are greater than that under plane stress or plane strain states, so it is unsafe to suppose a tensioned plate with finite thickness as one undergone plane stress or plane strain. For the sharper notch, the influence of three-dimensional stress state on the SCF must be considered.</P>

Time-oriented Perception of Environmental design

Zheng Shu Yang,Li Yan 국민대학교 동양문화디자인연구소 2010 Journal of Oriental Culture&Design Vol.2 No.1

Time-oriented Perception of Environmental design is based on the foundation of time and space. Following then key perception of environmental esthetics, design puts emphasis on the time-oriented experience. Because of the unique understanding of time and space in Eastem culture, Chinese traditiona1 architecture is the manifestation of this perception.

Impact of co-firing lean coal on NOx emission of a large-scale pulverized coal-fired utility boiler during partial load operation

Yang Zheng,Xiaotao Gao,Changdong Sheng 한국화학공학회 2017 Korean Journal of Chemical Engineering Vol.34 No.4

The present work aimed at combustion optimization of a 1,000MW tower-type ultra-supercritical boiler co-firing a lean coal with bituminous coals for reducing NOX emission particularly at low load operations. Historic operation data were systematically analyzed to investigate the characteristics of NOX emission. Through comparing between lean coal co-firing and sole bituminous coal firing, it was confirmed that, besides the big difference in quality and combustion characteristics of the lean coal from the bituminous coals, the excess air ratio in main combustion zone had a significant effect on NOX emission. Keeping the ratio at properly lower level achieved lower NOX emissions at low load operations. Based on the analyses, in-situ tests successfully brought NOX emissions of co-firing down close to 300mg/m3 at the load of 700MW, demonstrating the effectiveness of combustion optimization for controlling NOX emissions at partial load operation of co-firing.

Multivariate Analysis of the Prognosis of 37 Chondrosarcoma Patients

Yang, Zheng-Ming,Tao, Hui-Min,Ye, Zhao-Ming,Li, Wei-Xu,Lin, Nong,Yang, Di-Sheng Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.4

Objective: The current study aimedto screen for possible factors which affect prognosis of chondrosarcoma. Methods: Thirty seven cases were selected and analyzed statistically. The patients received surgical treatment at our hospital between December 2005 and March 2008. All of them had complete follow-up data. The survival rates were calculated by univariate analysis using the Kaplan-Meier method and tested by Log-rank. ${\chi}^2$ or Fisher exact tests were carried out for the numeration data. The significant indexes after univariate analysis were then analyzed by multivariate analysis using COX regression model. Based on the literature, factors of gender, age, disease course, tumor location, Enneking grades, surgical approaches, distant metastasis and local recurrence were examined. Results: Univariate analysis showed that there were significant differences in Enneking grades, surgical approaches and distant metastasis related to the patients' 3-year survival rate after surgery (P<0.001). No significant difference was not found in gender, age, disease course, tumor location or local recurrence (P>0.05). Multivariate analysis showed that Enneking grade (P=0.007) and surgical approaches (P=0.010) were independent factors affecting the prognosis of chondrosarcoma, but distant metastasis was not (P=0.942). Conclusion: Enneking grades, surgical approaches and distant metastasis are risk factors for prognosis of chondrosarcoma, among which the former two are independent factors.